hcov hku1  (ATCC)


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    ATCC hcov hku1
    Hcov Hku1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1  (ATCC)


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    ATCC hcov hku1
    Hcov Hku1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1 c n5  (ATCC)


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    ATCC hcov hku1 c n5
    Hcov Hku1 C N5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1 c n5  (ATCC)


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    ATCC hcov hku1 c n5
    Hcov Hku1 C N5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1 c n5  (ATCC)


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    ATCC hcov hku1 c n5
    Hcov Hku1 C N5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1 s  (ATCC)


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    ATCC hcov hku1 s
    A. Serum IgG binding titer against RBD (B.1.351). B. Comparison of serum neutralizing titers against VSV-SARS-CoV-2 Wu and B.1.351 at M1. Black horizontal line represents the mean, and the dashed line indicates no reduction. C. Frequency of Wu-specific and Wu+B.1.351 CR clonotypes in the RBD-pulldown. The total number of clonotypes is included at the center of each plot. D. Relative abundance of Wu+B.1.351 CR clonotypes in the RBD-pulldown. Relative abundance is calculated using the proteomics-based quantification of each antibody clonotype in serum. Mean values are represented as horizontal lines with the error bars indicating SD. E. Correlation between serum IgG binding titers to RBD (Wu) scaled by abundance of Wu+B.1.351 CR antibodies based on the proteomics data and serum IgG binding titers to RBD (B.1.351). F. Frequency of Wu-specific and Wu+B.1.351 CR antibody clonotypes that are S–RBD or iso– RBD. The error bars indicate SD. G. Serum IgG binding titer against <t>HKU1</t> S. H. Summary of <t>HKU1</t> <t>S</t> cross-reactive antibodies. For A and G , all samples run in technical duplicate, and the error bars indicate SD.
    Hcov Hku1 S, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Characterization of SARS-CoV-2 Convalescent Patients’ Serological Repertoire Reveals High Prevalence of Iso–RBD Antibodies"

    Article Title: Characterization of SARS-CoV-2 Convalescent Patients’ Serological Repertoire Reveals High Prevalence of Iso–RBD Antibodies

    Journal: bioRxiv

    doi: 10.1101/2023.09.08.556349

    A. Serum IgG binding titer against RBD (B.1.351). B. Comparison of serum neutralizing titers against VSV-SARS-CoV-2 Wu and B.1.351 at M1. Black horizontal line represents the mean, and the dashed line indicates no reduction. C. Frequency of Wu-specific and Wu+B.1.351 CR clonotypes in the RBD-pulldown. The total number of clonotypes is included at the center of each plot. D. Relative abundance of Wu+B.1.351 CR clonotypes in the RBD-pulldown. Relative abundance is calculated using the proteomics-based quantification of each antibody clonotype in serum. Mean values are represented as horizontal lines with the error bars indicating SD. E. Correlation between serum IgG binding titers to RBD (Wu) scaled by abundance of Wu+B.1.351 CR antibodies based on the proteomics data and serum IgG binding titers to RBD (B.1.351). F. Frequency of Wu-specific and Wu+B.1.351 CR antibody clonotypes that are S–RBD or iso– RBD. The error bars indicate SD. G. Serum IgG binding titer against HKU1 S. H. Summary of HKU1 S cross-reactive antibodies. For A and G , all samples run in technical duplicate, and the error bars indicate SD.
    Figure Legend Snippet: A. Serum IgG binding titer against RBD (B.1.351). B. Comparison of serum neutralizing titers against VSV-SARS-CoV-2 Wu and B.1.351 at M1. Black horizontal line represents the mean, and the dashed line indicates no reduction. C. Frequency of Wu-specific and Wu+B.1.351 CR clonotypes in the RBD-pulldown. The total number of clonotypes is included at the center of each plot. D. Relative abundance of Wu+B.1.351 CR clonotypes in the RBD-pulldown. Relative abundance is calculated using the proteomics-based quantification of each antibody clonotype in serum. Mean values are represented as horizontal lines with the error bars indicating SD. E. Correlation between serum IgG binding titers to RBD (Wu) scaled by abundance of Wu+B.1.351 CR antibodies based on the proteomics data and serum IgG binding titers to RBD (B.1.351). F. Frequency of Wu-specific and Wu+B.1.351 CR antibody clonotypes that are S–RBD or iso– RBD. The error bars indicate SD. G. Serum IgG binding titer against HKU1 S. H. Summary of HKU1 S cross-reactive antibodies. For A and G , all samples run in technical duplicate, and the error bars indicate SD.

    Techniques Used: Binding Assay, Comparison

    A. Binding specificity and affinity measured by ELISA. EC 50 values for each mAb to prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1.351), RBD (B.1.351), prefusion-stabilized S (B.1.1.529), and HKU1 S are shown. All assays were performed in triplicate. B-C. Neutralization activity of mAbs against pseudotyped VSV SARS-CoV-2 (Wu) ( B ) and VSV-SARS-CoV-2 (B.1.351) ( C ). CR3022, a non-neutralizing mAb was used as a negative control. The error bars indicate SD. D-F. Antibody-dependent cellular phagocytosis (ADCP) ( D ), FcγRIIIa activation (ADCC) ( E ), and antibody-dependent complement deposition (ADCD) ( F ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. REGN10987 IgG2σ was used as a null Fc-mediated effector function control. The dotted lines indicate the no antibody control.
    Figure Legend Snippet: A. Binding specificity and affinity measured by ELISA. EC 50 values for each mAb to prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1.351), RBD (B.1.351), prefusion-stabilized S (B.1.1.529), and HKU1 S are shown. All assays were performed in triplicate. B-C. Neutralization activity of mAbs against pseudotyped VSV SARS-CoV-2 (Wu) ( B ) and VSV-SARS-CoV-2 (B.1.351) ( C ). CR3022, a non-neutralizing mAb was used as a negative control. The error bars indicate SD. D-F. Antibody-dependent cellular phagocytosis (ADCP) ( D ), FcγRIIIa activation (ADCC) ( E ), and antibody-dependent complement deposition (ADCD) ( F ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. REGN10987 IgG2σ was used as a null Fc-mediated effector function control. The dotted lines indicate the no antibody control.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Activity Assay, Negative Control, Activation Assay

    Antibody-dependent cellular phagocytosis ( A ), FcγRIIIa activation ( B ), and antibody-dependent complement deposition ( C ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. The dotted line indicates the no antibody control, and REGN10987 IgG2σ was used as a null Fc-mediated effector function control.
    Figure Legend Snippet: Antibody-dependent cellular phagocytosis ( A ), FcγRIIIa activation ( B ), and antibody-dependent complement deposition ( C ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. The dotted line indicates the no antibody control, and REGN10987 IgG2σ was used as a null Fc-mediated effector function control.

    Techniques Used: Activation Assay, Activity Assay

    hcov hku1 c n5  (ATCC)


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    ATCC hcov hku1 c n5
    Hcov Hku1 C N5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1 c n5  (ATCC)


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    ATCC hcov hku1 c n5
    Hcov Hku1 C N5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1 c n5  (ATCC)


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    ATCC hcov hku1 c n5
    Hcov Hku1 C N5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1  (ATCC)


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    ATCC hcov hku1
    Hcov Hku1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov hku1  (ATCC)


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    ATCC hcov hku1
    <t> Human coronavirus </t> in vitro properties.
    Hcov Hku1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Broad-spectrum coronavirus antiviral drug discovery"

    Article Title: Broad-spectrum coronavirus antiviral drug discovery

    Journal: Expert Opinion on Drug Discovery

    doi: 10.1080/17460441.2019.1581171

     Human coronavirus  in vitro properties.
    Figure Legend Snippet: Human coronavirus in vitro properties.

    Techniques Used: In Vitro

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    ATCC hcov hku1 s
    A. Serum IgG binding titer against RBD (B.1.351). B. Comparison of serum neutralizing titers against VSV-SARS-CoV-2 Wu and B.1.351 at M1. Black horizontal line represents the mean, and the dashed line indicates no reduction. C. Frequency of Wu-specific and Wu+B.1.351 CR clonotypes in the RBD-pulldown. The total number of clonotypes is included at the center of each plot. D. Relative abundance of Wu+B.1.351 CR clonotypes in the RBD-pulldown. Relative abundance is calculated using the proteomics-based quantification of each antibody clonotype in serum. Mean values are represented as horizontal lines with the error bars indicating SD. E. Correlation between serum IgG binding titers to RBD (Wu) scaled by abundance of Wu+B.1.351 CR antibodies based on the proteomics data and serum IgG binding titers to RBD (B.1.351). F. Frequency of Wu-specific and Wu+B.1.351 CR antibody clonotypes that are S–RBD or iso– RBD. The error bars indicate SD. G. Serum IgG binding titer against <t>HKU1</t> S. H. Summary of <t>HKU1</t> <t>S</t> cross-reactive antibodies. For A and G , all samples run in technical duplicate, and the error bars indicate SD.
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    A. Serum IgG binding titer against RBD (B.1.351). B. Comparison of serum neutralizing titers against VSV-SARS-CoV-2 Wu and B.1.351 at M1. Black horizontal line represents the mean, and the dashed line indicates no reduction. C. Frequency of Wu-specific and Wu+B.1.351 CR clonotypes in the RBD-pulldown. The total number of clonotypes is included at the center of each plot. D. Relative abundance of Wu+B.1.351 CR clonotypes in the RBD-pulldown. Relative abundance is calculated using the proteomics-based quantification of each antibody clonotype in serum. Mean values are represented as horizontal lines with the error bars indicating SD. E. Correlation between serum IgG binding titers to RBD (Wu) scaled by abundance of Wu+B.1.351 CR antibodies based on the proteomics data and serum IgG binding titers to RBD (B.1.351). F. Frequency of Wu-specific and Wu+B.1.351 CR antibody clonotypes that are S–RBD or iso– RBD. The error bars indicate SD. G. Serum IgG binding titer against HKU1 S. H. Summary of HKU1 S cross-reactive antibodies. For A and G , all samples run in technical duplicate, and the error bars indicate SD.

    Journal: bioRxiv

    Article Title: Characterization of SARS-CoV-2 Convalescent Patients’ Serological Repertoire Reveals High Prevalence of Iso–RBD Antibodies

    doi: 10.1101/2023.09.08.556349

    Figure Lengend Snippet: A. Serum IgG binding titer against RBD (B.1.351). B. Comparison of serum neutralizing titers against VSV-SARS-CoV-2 Wu and B.1.351 at M1. Black horizontal line represents the mean, and the dashed line indicates no reduction. C. Frequency of Wu-specific and Wu+B.1.351 CR clonotypes in the RBD-pulldown. The total number of clonotypes is included at the center of each plot. D. Relative abundance of Wu+B.1.351 CR clonotypes in the RBD-pulldown. Relative abundance is calculated using the proteomics-based quantification of each antibody clonotype in serum. Mean values are represented as horizontal lines with the error bars indicating SD. E. Correlation between serum IgG binding titers to RBD (Wu) scaled by abundance of Wu+B.1.351 CR antibodies based on the proteomics data and serum IgG binding titers to RBD (B.1.351). F. Frequency of Wu-specific and Wu+B.1.351 CR antibody clonotypes that are S–RBD or iso– RBD. The error bars indicate SD. G. Serum IgG binding titer against HKU1 S. H. Summary of HKU1 S cross-reactive antibodies. For A and G , all samples run in technical duplicate, and the error bars indicate SD.

    Article Snippet: Briefly, 500,000 1 μm yellow-green fluorescent microspheres (Thermo, F8813) were covalently conjugated with recombinant SARS-CoV-2 S, SARS-CoV-2 RBD, SARS-CoV-2 S (B.1.351), SARS-CoV-2 RBD (B.1.351), and hCoV HKU1 S and incubated for 3 hr with diluted recombinant mAbs (3-fold dilution, 5 μg/mL to 6.8 ng/mL) and 20,000 cells from the human monocytic THP-1 cell line (ATCC, TIB-202).

    Techniques: Binding Assay, Comparison

    A. Binding specificity and affinity measured by ELISA. EC 50 values for each mAb to prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1.351), RBD (B.1.351), prefusion-stabilized S (B.1.1.529), and HKU1 S are shown. All assays were performed in triplicate. B-C. Neutralization activity of mAbs against pseudotyped VSV SARS-CoV-2 (Wu) ( B ) and VSV-SARS-CoV-2 (B.1.351) ( C ). CR3022, a non-neutralizing mAb was used as a negative control. The error bars indicate SD. D-F. Antibody-dependent cellular phagocytosis (ADCP) ( D ), FcγRIIIa activation (ADCC) ( E ), and antibody-dependent complement deposition (ADCD) ( F ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. REGN10987 IgG2σ was used as a null Fc-mediated effector function control. The dotted lines indicate the no antibody control.

    Journal: bioRxiv

    Article Title: Characterization of SARS-CoV-2 Convalescent Patients’ Serological Repertoire Reveals High Prevalence of Iso–RBD Antibodies

    doi: 10.1101/2023.09.08.556349

    Figure Lengend Snippet: A. Binding specificity and affinity measured by ELISA. EC 50 values for each mAb to prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1.351), RBD (B.1.351), prefusion-stabilized S (B.1.1.529), and HKU1 S are shown. All assays were performed in triplicate. B-C. Neutralization activity of mAbs against pseudotyped VSV SARS-CoV-2 (Wu) ( B ) and VSV-SARS-CoV-2 (B.1.351) ( C ). CR3022, a non-neutralizing mAb was used as a negative control. The error bars indicate SD. D-F. Antibody-dependent cellular phagocytosis (ADCP) ( D ), FcγRIIIa activation (ADCC) ( E ), and antibody-dependent complement deposition (ADCD) ( F ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. REGN10987 IgG2σ was used as a null Fc-mediated effector function control. The dotted lines indicate the no antibody control.

    Article Snippet: Briefly, 500,000 1 μm yellow-green fluorescent microspheres (Thermo, F8813) were covalently conjugated with recombinant SARS-CoV-2 S, SARS-CoV-2 RBD, SARS-CoV-2 S (B.1.351), SARS-CoV-2 RBD (B.1.351), and hCoV HKU1 S and incubated for 3 hr with diluted recombinant mAbs (3-fold dilution, 5 μg/mL to 6.8 ng/mL) and 20,000 cells from the human monocytic THP-1 cell line (ATCC, TIB-202).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Activity Assay, Negative Control, Activation Assay

    Antibody-dependent cellular phagocytosis ( A ), FcγRIIIa activation ( B ), and antibody-dependent complement deposition ( C ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. The dotted line indicates the no antibody control, and REGN10987 IgG2σ was used as a null Fc-mediated effector function control.

    Journal: bioRxiv

    Article Title: Characterization of SARS-CoV-2 Convalescent Patients’ Serological Repertoire Reveals High Prevalence of Iso–RBD Antibodies

    doi: 10.1101/2023.09.08.556349

    Figure Lengend Snippet: Antibody-dependent cellular phagocytosis ( A ), FcγRIIIa activation ( B ), and antibody-dependent complement deposition ( C ) activity of mAbs against SARS-CoV-2 prefusion-stabilized S (Wu), RBD (Wu), prefusion-stabilized S (B.1351), and HKU1 S. The dotted line indicates the no antibody control, and REGN10987 IgG2σ was used as a null Fc-mediated effector function control.

    Article Snippet: Briefly, 500,000 1 μm yellow-green fluorescent microspheres (Thermo, F8813) were covalently conjugated with recombinant SARS-CoV-2 S, SARS-CoV-2 RBD, SARS-CoV-2 S (B.1.351), SARS-CoV-2 RBD (B.1.351), and hCoV HKU1 S and incubated for 3 hr with diluted recombinant mAbs (3-fold dilution, 5 μg/mL to 6.8 ng/mL) and 20,000 cells from the human monocytic THP-1 cell line (ATCC, TIB-202).

    Techniques: Activation Assay, Activity Assay