hcn2  (Alomone Labs)


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    Structured Review

    Alomone Labs hcn2
    HCN channels are present on GABAergic terminals in the mPFC. (A) Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrate the cells in layers 5–6 of the mPFC. Scale bar: 40 µm. (B,C) Single-plane confocal images showing the HCN1-ir (B1), <t>HCN2-ir</t> (B2), HCN4-ir (B3), and GAD65-ir (C1-C3) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). (D) Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bar: 20 µm.
    Hcn2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex"

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    Journal: Biology Open

    doi: 10.1242/bio.058840

    HCN channels are present on GABAergic terminals in the mPFC. (A) Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrate the cells in layers 5–6 of the mPFC. Scale bar: 40 µm. (B,C) Single-plane confocal images showing the HCN1-ir (B1), HCN2-ir (B2), HCN4-ir (B3), and GAD65-ir (C1-C3) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). (D) Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bar: 20 µm.
    Figure Legend Snippet: HCN channels are present on GABAergic terminals in the mPFC. (A) Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrate the cells in layers 5–6 of the mPFC. Scale bar: 40 µm. (B,C) Single-plane confocal images showing the HCN1-ir (B1), HCN2-ir (B2), HCN4-ir (B3), and GAD65-ir (C1-C3) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). (D) Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bar: 20 µm.

    Techniques Used: Staining, Marker, Labeling

    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layers 5–6 of mPFC. (A–C) Microscopic confocal images showing HCN1-ir (A), HCN2-ir (B), and HCN4-ir (C) locate in PV-ir interneuron in layers 5–6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bars: 20 µm. (D) High-magnification confocal microscopy images showing that HCN1-ir localize in the soma (d1) and along neurite (d2-d3) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite (d1) is digitally magnified for a better view of neurite in (d2) and (d3), respectively. Scale bars: 20 µm in (d1) and 1 µm in (d2) and (d3). (E) High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digitally magnified for a better view of neurite in (e2). Scale bars: 20 µm in (e1) and 1 µm in (e2).
    Figure Legend Snippet: HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layers 5–6 of mPFC. (A–C) Microscopic confocal images showing HCN1-ir (A), HCN2-ir (B), and HCN4-ir (C) locate in PV-ir interneuron in layers 5–6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bars: 20 µm. (D) High-magnification confocal microscopy images showing that HCN1-ir localize in the soma (d1) and along neurite (d2-d3) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite (d1) is digitally magnified for a better view of neurite in (d2) and (d3), respectively. Scale bars: 20 µm in (d1) and 1 µm in (d2) and (d3). (E) High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digitally magnified for a better view of neurite in (e2). Scale bars: 20 µm in (e1) and 1 µm in (e2).

    Techniques Used: Expressing, Staining, Labeling, Confocal Microscopy

    2) Product Images from "Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex"

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    Journal: Biology Open

    doi: 10.1242/bio.058840

    HCN channels are present on GABAergic terminals in the mPFC. (A) Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrate the cells in layers 5–6 of the mPFC. Scale bar: 40 µm. (B,C) Single-plane confocal images showing the HCN1-ir (B1), HCN2-ir (B2), HCN4-ir (B3), and GAD65-ir (C1-C3) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). (D) Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bar: 20 µm.
    Figure Legend Snippet: HCN channels are present on GABAergic terminals in the mPFC. (A) Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrate the cells in layers 5–6 of the mPFC. Scale bar: 40 µm. (B,C) Single-plane confocal images showing the HCN1-ir (B1), HCN2-ir (B2), HCN4-ir (B3), and GAD65-ir (C1-C3) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). (D) Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bar: 20 µm.

    Techniques Used: Staining, Marker, Labeling

    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layers 5–6 of mPFC. (A–C) Microscopic confocal images showing HCN1-ir (A), HCN2-ir (B), and HCN4-ir (C) locate in PV-ir interneuron in layers 5–6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bars: 20 µm. (D) High-magnification confocal microscopy images showing that HCN1-ir localize in the soma (d1) and along neurite (d2-d3) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite (d1) is digitally magnified for a better view of neurite in (d2) and (d3), respectively. Scale bars: 20 µm in (d1) and 1 µm in (d2) and (d3). (E) High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digitally magnified for a better view of neurite in (e2). Scale bars: 20 µm in (e1) and 1 µm in (e2).
    Figure Legend Snippet: HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layers 5–6 of mPFC. (A–C) Microscopic confocal images showing HCN1-ir (A), HCN2-ir (B), and HCN4-ir (C) locate in PV-ir interneuron in layers 5–6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bars: 20 µm. (D) High-magnification confocal microscopy images showing that HCN1-ir localize in the soma (d1) and along neurite (d2-d3) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite (d1) is digitally magnified for a better view of neurite in (d2) and (d3), respectively. Scale bars: 20 µm in (d1) and 1 µm in (d2) and (d3). (E) High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digitally magnified for a better view of neurite in (e2). Scale bars: 20 µm in (e1) and 1 µm in (e2).

    Techniques Used: Expressing, Staining, Labeling, Confocal Microscopy

    3) Product Images from "Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex"

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    Journal: bioRxiv

    doi: 10.1101/2021.05.23.445332

    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).
    Figure Legend Snippet: HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).

    Techniques Used: Expressing, Staining, Labeling, Confocal Microscopy

    HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.
    Figure Legend Snippet: HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.

    Techniques Used: Staining, Marker, Labeling

    4) Product Images from "Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex"

    Article Title: Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex

    Journal: bioRxiv

    doi: 10.1101/2021.05.23.445332

    HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).
    Figure Legend Snippet: HCN channels are present in soma and neurite of parvalbumin-expressing basket cells in layer 5-6 of mPFC. A-C. Microscopic confocal images showing HCN1-ir ( A ), HCN2-ir ( B ), and HCN4-ir ( C ) locate in PV-ir interneuron in layer 5-6 of mPFC. Double stained with HCN channels (red) and PV (green). Arrowheads indicate double-labeled cells. Scale bar, 20 μm D. High-magnification confocal microscopy images showing that HCN1-ir localize in the soma ( d1 ) and along neurite ( d2-d3 ) of PV-ir interneuron. Silhouette frame 1 and 2 in neurite ( d1 ) is digital magnified for better view of neurite in ( d2 ) and ( d3 ), respectively. Scale bars, 20 μm in ( d1 ) and 1 μm in ( d2 ) and ( d3 ). E. High-magnification confocal microscopy images showing that HCN2-ir localize in the soma and along neurite of PV-ir interneuron. Silhouette frame in neurite (e1) is digital magnified for better view of neurite in (e2). Scale bars, 20 μm in (e1) and 1 μm in (e2).

    Techniques Used: Expressing, Staining, Labeling, Confocal Microscopy

    HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.
    Figure Legend Snippet: HCN channels are present on GABAergic terminals in the mPFC. A. Low-magnification confocal images showing double stained with HCN channels (red) and GAD65 (green), a GABAergic terminal marker. The squares illustrating the cells in layer 5-6 of the mPFC. Scale bar, 40 μm B and C. Single-plane confocal images showing the HCN1-ir ( B1 ), HCN2-ir ( B2 ), HCN4-ir ( B3 ), and GAD65-ir ( C1-C3 ) at high magnification. GAD65-ir appears in punctuate structures distributed in the neuropil, as well as around unlabeled pyramidal cell soma (C1-C3). E. Merging of the paired images (B1 and C1), (B2 and C2), and (B3 and C3) shows that the puncta of GAD65-ir surround the cell bodies of HCN1-ir (B1), HCN2-ir (B2), and HCN4-ir (B3) cells. Partially overlapping areas of red (HCN) and green (GAD65) profiles showing yellow. The arrowheads indicate double-labeled cells. Scale bars represent 20 μm.

    Techniques Used: Staining, Marker, Labeling

    5) Product Images from "Characterization of small fiber pathology in a mouse model of Fabry disease"

    Article Title: Characterization of small fiber pathology in a mouse model of Fabry disease

    Journal: eLife

    doi: 10.7554/eLife.39300

    Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p
    Figure Legend Snippet: Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p

    Techniques Used: Expressing, Mouse Assay

    6) Product Images from "Characterization of small fiber pathology in a mouse model of Fabry disease"

    Article Title: Characterization of small fiber pathology in a mouse model of Fabry disease

    Journal: eLife

    doi: 10.7554/eLife.39300

    Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p
    Figure Legend Snippet: Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p

    Techniques Used: Expressing, Mouse Assay

    7) Product Images from "Characterization of small fiber pathology in a mouse model of Fabry disease"

    Article Title: Characterization of small fiber pathology in a mouse model of Fabry disease

    Journal: eLife

    doi: 10.7554/eLife.39300

    Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p
    Figure Legend Snippet: Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p

    Techniques Used: Expressing, Mouse Assay

    8) Product Images from "Characterization of small fiber pathology in a mouse model of Fabry disease"

    Article Title: Characterization of small fiber pathology in a mouse model of Fabry disease

    Journal: eLife

    doi: 10.7554/eLife.39300

    Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p
    Figure Legend Snippet: Expression, function, and phenotypic reflection of hyperpolarization-activated cyclic nucleotide-gated ion channels in α-galactosidase A deficient mice. ( A ) Boxplots show the results of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene expression in dorsal root ganglia (DRG) of young (3 months) and old (≥12 months) wildtype (WT) and α-galactosidase A deficient (GLA KO) mice. No intergroup difference was found. ( B–E ) Photomicrographs illustrate immunoreactivity of antibodies against HCN2 in DRG of young and old WT and GLA KO mice; ( F ) shows the result of quantification. Old GLA KO and WT mice showed greated HCN2 immunoreactivity compared to young littermates (p

    Techniques Used: Expressing, Mouse Assay

    9) Product Images from "Adenosine and dopamine oppositely modulate a hyperpolarization‐activated current I h in chemosensory neurons of the rat carotid body in co‐culture"

    Article Title: Adenosine and dopamine oppositely modulate a hyperpolarization‐activated current I h in chemosensory neurons of the rat carotid body in co‐culture

    Journal: The Journal of Physiology

    doi: 10.1113/JP274743

    Localization of HCN4 and HCN2 subunits in tissue sections of rat petrosal ganglia In A , confocal immunofluorescence labelling revealed that HCN4 immunoreactivity (HCN4‐ir) is present in many petrosal neurons, and co‐localizes with several neurons expressing tyrosine hydroxylase (TH)‐ir ( B ), a marker for chemoafferent neurons; merged images are shown in C . Note co‐localization of TH‐ir and HCN4‐ir in several neurons in boxed area ( n = 3). In D , many petrosal neurons are positive for HCN2‐ir, but there is no evidence for a significant co‐localization with TH‐ir in the boxed region ( E and F ; n = 3). In control experiments omission of the primary antibody, or preincubation with blocking peptide (see Methods), resulted in the absence of all immunostaining ( n ]
    Figure Legend Snippet: Localization of HCN4 and HCN2 subunits in tissue sections of rat petrosal ganglia In A , confocal immunofluorescence labelling revealed that HCN4 immunoreactivity (HCN4‐ir) is present in many petrosal neurons, and co‐localizes with several neurons expressing tyrosine hydroxylase (TH)‐ir ( B ), a marker for chemoafferent neurons; merged images are shown in C . Note co‐localization of TH‐ir and HCN4‐ir in several neurons in boxed area ( n = 3). In D , many petrosal neurons are positive for HCN2‐ir, but there is no evidence for a significant co‐localization with TH‐ir in the boxed region ( E and F ; n = 3). In control experiments omission of the primary antibody, or preincubation with blocking peptide (see Methods), resulted in the absence of all immunostaining ( n ]

    Techniques Used: Immunofluorescence, Expressing, Marker, Blocking Assay, Immunostaining

    10) Product Images from "Differential expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits during hippocampal development in the mouse"

    Article Title: Differential expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits during hippocampal development in the mouse

    Journal: Molecular Brain

    doi: 10.1186/s13041-015-0103-4

    Expression of HCN2 in PV-positive cells of the hippocampus at P21. (A-B, D-F, H-J, L-N, P) : The images show HCN2 labeling in different fields of the hippocampus and the DG. (A, E, I) : Note that HCN2 expression was more prominent in the sl-m and sp of the CA, particularly in CA3. Interestingly, the alveus did not show HCN2 expression at this developmental stage. In addition, the sl-m and sp of the CA3 (I) presented stronger labeling than in the CA1 (A) and the CA2 (E) . Most PV-immunopositive cells located in the sp of the CA1 (A, B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and hilus of the DG (M, N, P) co-expressed HCN2. A - B , D - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN2 in PV-positive cells of the hippocampus at P21. (A-B, D-F, H-J, L-N, P) : The images show HCN2 labeling in different fields of the hippocampus and the DG. (A, E, I) : Note that HCN2 expression was more prominent in the sl-m and sp of the CA, particularly in CA3. Interestingly, the alveus did not show HCN2 expression at this developmental stage. In addition, the sl-m and sp of the CA3 (I) presented stronger labeling than in the CA1 (A) and the CA2 (E) . Most PV-immunopositive cells located in the sp of the CA1 (A, B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and hilus of the DG (M, N, P) co-expressed HCN2. A - B , D - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.

    Techniques Used: Expressing, Labeling, Staining

    Expression of HCN subunits in the hippocampus at P0 and P7. (A-1) : At P0, the expression of HCN1 was strong in the alveus and so , sp , sl , and sr of the CA, and in the GCL of the dentate gyrus (DG). (E-1) : HCN2 expression at P0 was observed in the alveus , sp , sl , and sr of the CA, and in the GCL of the DG. (I-1) : HCN4 showed a similar pattern of expression to HCN1 at P0. Note that migrating cells (yellow arrows) from the ventricular zone (vz, white arrows) expressed all HCN isoforms, but the expression of HCN1 and HCN2 subunits was more prominent than that of HCN4. At P7, immunolabeling for HCN1 (A-2) , HCN2 (E-2) , and HCN4 (I-2) was observed in the sp and slm of the CA, and in the gcl of the DG. HCN1 (B, C, D) and HCN2 (F, G, H) subunits were expressed in neuronal somata, but not in astrocytes. Labeling for HCN4 was observed in the slm , as well as in the border of the slm with the molecular layer ( ml ) of the DG. (B, F, J) : Double immunofluorescence with GFAP showed that most GFAP-positive astrocytes were also immunolabeled with HCN4 in the slm, and in the border of the ml in the DG. B , C , D : yellow arrows indicate HCN1 labeling, white arrows indicate GFAP labeling. F , G , H : yellow arrows indicate HCN2 labeling. J , K , L : white arrow indicates an astrocyte double-labeled for HCN4 and GFAP. Abbreviations: GCL (or gcl), granule cell layer; sl, stratum lucidum; slm, stratum lacunosum moleculare; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN subunits in the hippocampus at P0 and P7. (A-1) : At P0, the expression of HCN1 was strong in the alveus and so , sp , sl , and sr of the CA, and in the GCL of the dentate gyrus (DG). (E-1) : HCN2 expression at P0 was observed in the alveus , sp , sl , and sr of the CA, and in the GCL of the DG. (I-1) : HCN4 showed a similar pattern of expression to HCN1 at P0. Note that migrating cells (yellow arrows) from the ventricular zone (vz, white arrows) expressed all HCN isoforms, but the expression of HCN1 and HCN2 subunits was more prominent than that of HCN4. At P7, immunolabeling for HCN1 (A-2) , HCN2 (E-2) , and HCN4 (I-2) was observed in the sp and slm of the CA, and in the gcl of the DG. HCN1 (B, C, D) and HCN2 (F, G, H) subunits were expressed in neuronal somata, but not in astrocytes. Labeling for HCN4 was observed in the slm , as well as in the border of the slm with the molecular layer ( ml ) of the DG. (B, F, J) : Double immunofluorescence with GFAP showed that most GFAP-positive astrocytes were also immunolabeled with HCN4 in the slm, and in the border of the ml in the DG. B , C , D : yellow arrows indicate HCN1 labeling, white arrows indicate GFAP labeling. F , G , H : yellow arrows indicate HCN2 labeling. J , K , L : white arrow indicates an astrocyte double-labeled for HCN4 and GFAP. Abbreviations: GCL (or gcl), granule cell layer; sl, stratum lucidum; slm, stratum lacunosum moleculare; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. Scale bars = 20 μm.

    Techniques Used: Expressing, Immunolabeling, Labeling, Immunofluorescence

    Expression of HCN2 in PV-positive cells of the hippocampus at P56. (A-B, D, E-F, H-J, L-N, P) : Most PV-immunopositive cells located in the CA1 (A-B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and DG (M, N, P) were labeled with HCN2. (A, E, I) : HCN2 expression was most prominent in the sl-m of the CA1 (A) , as well as in the sp of the CA2 (E) and CA3 (I) . Interestingly, at this stage the alveus showed HCN2 labeling, contrasting with its expression pattern at P21. A - B , D , E - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN2 in PV-positive cells of the hippocampus at P56. (A-B, D, E-F, H-J, L-N, P) : Most PV-immunopositive cells located in the CA1 (A-B, D) , CA2 (E, F, H) , CA3 (I, J, L) , and DG (M, N, P) were labeled with HCN2. (A, E, I) : HCN2 expression was most prominent in the sl-m of the CA1 (A) , as well as in the sp of the CA2 (E) and CA3 (I) . Interestingly, at this stage the alveus showed HCN2 labeling, contrasting with its expression pattern at P21. A - B , D , E - F , H - J , L - N , P : white arrows indicate PV-positive/HCN2-positive cells. C , G , K , O : DAPI staining. Scale bars = 20 μm.

    Techniques Used: Expressing, Labeling, Staining

    Expression of HCN subunits in the hippocampal region at E14.5. HCN1 (A) , HCN2 (D) , and HCN4 (G) immunolabeling was more evident in the intermediate zone (iz) than in the ventricular zone (vz). In the hippocampal iz, labeling for HCN2 and HCN4 subunits was higher than for HCN1. B , E , H : DAPI staining in the nucleus. C , F , I : merged images of HCN immunolabeling and DAPI stain. Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN subunits in the hippocampal region at E14.5. HCN1 (A) , HCN2 (D) , and HCN4 (G) immunolabeling was more evident in the intermediate zone (iz) than in the ventricular zone (vz). In the hippocampal iz, labeling for HCN2 and HCN4 subunits was higher than for HCN1. B , E , H : DAPI staining in the nucleus. C , F , I : merged images of HCN immunolabeling and DAPI stain. Scale bars = 20 μm.

    Techniques Used: Expressing, Immunolabeling, Labeling, Staining

    Expression of HCN subunits in doublecortin (DCX)-positive cells of the dentate gyrus at P21. (A-L) : These images show our results on double immunofluorescence for HCN subunits and DCX. In the DCX-immunopositive subgranular zone we did not find labeling for HCN1 (A-D) , HCN2 (E-H) , or HCN4 (I-L) . Scale bars = 20 μm.
    Figure Legend Snippet: Expression of HCN subunits in doublecortin (DCX)-positive cells of the dentate gyrus at P21. (A-L) : These images show our results on double immunofluorescence for HCN subunits and DCX. In the DCX-immunopositive subgranular zone we did not find labeling for HCN1 (A-D) , HCN2 (E-H) , or HCN4 (I-L) . Scale bars = 20 μm.

    Techniques Used: Expressing, Immunofluorescence, Labeling

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    Alomone Labs polyclonal rabbit rb anti hcn2
    Alteration in HCN channel expression in thalamus following general demyelination. ( A , B ) Immunofluorescence staining of the VB complex (horizontal thalamic sections, 40 μm) comparing the expression of <t>HCN4</t> (A, in red, <t>rb-anti-HCN4,</t> 1:200, Alomone) and <t>HCN2</t> (B, in red, rb-anti-HCN4, 1:200, Alomone) channels between control C3H/HeJ and Day1. The purified ms-anti- neurofilament antibody (SMI312, pan axonal, 1:200, BioLegend) was used as an axonal marker (SMI312, in green). Cell nuclei were stained with DAPI (in blue). ( C , D ) Bar graphs comparing the intensity of the fluorescence signal (using integrated fluorescence intensity values) for SMI312 (C and D upper traces) and HCN4 and HCN2 (lower traces) between the control C3H/HeJ and Day1. ( E ) Immunofluorescence staining of VB in control C3H/HeJ and Day1 with antibodies against TRIP8b (ms-anti-(constant) TRIP8b, 1:50, NeuroMab, in green) and phosphorylated TRIP8b (rb-α-pS237 antibody, 1:100, YenZym). ( F ) Representative bar graph comparing the intensity of the fluorescence signal for total TRIP8b and pS237 between the two groups indicating a significant reduction for phosphorylated TRIP8b, pS237, on Day1. Scale bars indicate 100 μm. VPL, VPM, and TRN stand for ventral-posterior medial, ventral-posterior lateral, and thalamic reticular nucleus, respectively.
    Polyclonal Rabbit Rb Anti Hcn2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti hcn2 antibody
    Effect of chronic training on HCN1, KCN2 and HCN4 protein expression determined by immunocytochemical techniques in sedentary and trained dog. Bar diagram on top left indicates that the relative density of dog cardiomyocytes with HCN4 immunolabelling obtained from the trained group (n=30 cells/6 dogs) is significantly increased compared to that measured in the sedentary group (n=30 cells/6 dogs). Original immunofluorescent images are shown on the left . Bottom panels indicate lack of effect of chronic training on the relative density of dog cardiomyocytes with HCN1 and <t>HCN2</t> immunolabelling (n=30 – 30 cells/6 – 6 dogs for sedentary and trained groups, respectively). Figure 7–Source Data 1 Effect of chronic training on HCN4 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 2 Effect of chronic training on HCN1 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 3 Effect of chronic training on HCN2 protein expression determined by immunocytochemical technique in sedentary and trained dogs.
    Anti Hcn2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alteration in HCN channel expression in thalamus following general demyelination. ( A , B ) Immunofluorescence staining of the VB complex (horizontal thalamic sections, 40 μm) comparing the expression of HCN4 (A, in red, rb-anti-HCN4, 1:200, Alomone) and HCN2 (B, in red, rb-anti-HCN4, 1:200, Alomone) channels between control C3H/HeJ and Day1. The purified ms-anti- neurofilament antibody (SMI312, pan axonal, 1:200, BioLegend) was used as an axonal marker (SMI312, in green). Cell nuclei were stained with DAPI (in blue). ( C , D ) Bar graphs comparing the intensity of the fluorescence signal (using integrated fluorescence intensity values) for SMI312 (C and D upper traces) and HCN4 and HCN2 (lower traces) between the control C3H/HeJ and Day1. ( E ) Immunofluorescence staining of VB in control C3H/HeJ and Day1 with antibodies against TRIP8b (ms-anti-(constant) TRIP8b, 1:50, NeuroMab, in green) and phosphorylated TRIP8b (rb-α-pS237 antibody, 1:100, YenZym). ( F ) Representative bar graph comparing the intensity of the fluorescence signal for total TRIP8b and pS237 between the two groups indicating a significant reduction for phosphorylated TRIP8b, pS237, on Day1. Scale bars indicate 100 μm. VPL, VPM, and TRN stand for ventral-posterior medial, ventral-posterior lateral, and thalamic reticular nucleus, respectively.

    Journal: Cerebral Cortex (New York, NY)

    Article Title: Modulation of pacemaker channel function in a model of thalamocortical hyperexcitability by demyelination and cytokines

    doi: 10.1093/cercor/bhab491

    Figure Lengend Snippet: Alteration in HCN channel expression in thalamus following general demyelination. ( A , B ) Immunofluorescence staining of the VB complex (horizontal thalamic sections, 40 μm) comparing the expression of HCN4 (A, in red, rb-anti-HCN4, 1:200, Alomone) and HCN2 (B, in red, rb-anti-HCN4, 1:200, Alomone) channels between control C3H/HeJ and Day1. The purified ms-anti- neurofilament antibody (SMI312, pan axonal, 1:200, BioLegend) was used as an axonal marker (SMI312, in green). Cell nuclei were stained with DAPI (in blue). ( C , D ) Bar graphs comparing the intensity of the fluorescence signal (using integrated fluorescence intensity values) for SMI312 (C and D upper traces) and HCN4 and HCN2 (lower traces) between the control C3H/HeJ and Day1. ( E ) Immunofluorescence staining of VB in control C3H/HeJ and Day1 with antibodies against TRIP8b (ms-anti-(constant) TRIP8b, 1:50, NeuroMab, in green) and phosphorylated TRIP8b (rb-α-pS237 antibody, 1:100, YenZym). ( F ) Representative bar graph comparing the intensity of the fluorescence signal for total TRIP8b and pS237 between the two groups indicating a significant reduction for phosphorylated TRIP8b, pS237, on Day1. Scale bars indicate 100 μm. VPL, VPM, and TRN stand for ventral-posterior medial, ventral-posterior lateral, and thalamic reticular nucleus, respectively.

    Article Snippet: Sections were washed three times for 10 min in PBS and incubated for 2 h in blocking solution (10% normal goat serum, 3% bovine serum albumin (BSA), 0.3% Triton-X100 in PBS) followed by 48 h of incubation at 4 °C with the following primary antibodies: polyclonal rabbit (rb)-anti-HCN2 (1:200), rb-anti-HCN4 (1:200; Alomone Labs), rb-anti-NeuN (neuronal specific marker, 1:1000; Abcam), mouse purified (ms)-anti-neurofilament marker (pan axonal, cocktail, SMI312; 1:200, BioLegend) and ms-anti-Parvalbumin (PV235, 1:500; Swant).

    Techniques: Expressing, Immunofluorescence, Staining, Purification, Marker, Fluorescence

    Human lymphatic vessels predominantly express HCN2. (a) RT‐PCR analysis of HCN2 (229 base pairs) and HCN3 (589 bp) amplified from thoracic duct (TD) and mesenteric lymphatic vessels (ML); HCN1 (597 bp) and HCN4 (232 bp) were consistently amplified from the control human RNA, dorsal root ganglia (DRG) and heart, respectively, while all lymphatic samples were negative. Samples are presented pairwise as reverse transcriptase positive (+) followed by reverse transcriptase negative (−). HCN2 immunoreactivity (red fluorescence, left panels) was observed in smooth muscle cells of (b) human thoracic duct and (c) mesenteric lymphatic vessels. (d) Antibody specificity was confirmed by the absence of staining when the antibody was preincubated with peptide control (green fluorescent nuclear stain). Scale bars denote 50 μm (top) and 100 μm (middle and bottom), * indicates lumen, and right panels present differential interference contrast (DiC) images of the same section.

    Journal: Physiological Reports

    Article Title: Positive chronotropic action of HCN channel antagonism in human collecting lymphatic vessels). Positive chronotropic action of HCN channel antagonism in human collecting lymphatic vessels

    doi: 10.14814/phy2.15401

    Figure Lengend Snippet: Human lymphatic vessels predominantly express HCN2. (a) RT‐PCR analysis of HCN2 (229 base pairs) and HCN3 (589 bp) amplified from thoracic duct (TD) and mesenteric lymphatic vessels (ML); HCN1 (597 bp) and HCN4 (232 bp) were consistently amplified from the control human RNA, dorsal root ganglia (DRG) and heart, respectively, while all lymphatic samples were negative. Samples are presented pairwise as reverse transcriptase positive (+) followed by reverse transcriptase negative (−). HCN2 immunoreactivity (red fluorescence, left panels) was observed in smooth muscle cells of (b) human thoracic duct and (c) mesenteric lymphatic vessels. (d) Antibody specificity was confirmed by the absence of staining when the antibody was preincubated with peptide control (green fluorescent nuclear stain). Scale bars denote 50 μm (top) and 100 μm (middle and bottom), * indicates lumen, and right panels present differential interference contrast (DiC) images of the same section.

    Article Snippet: Thereafter, tissue was permeabilized with 0.25% Triton X‐100 in PBS for 10 min following by blocking with 2% bovine serum albumin (BSA) in PBS for 20 min. Tissue was incubated overnight in a dark, humidified chamber at 4°C with 1:50 rabbit polyclonal anti‐HCN2 (APC‐030‐AR; Alomone, Israel) preconjugated to ATTO‐594 fluorophore.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Fluorescence, Staining

    Effect of chronic training on HCN1, KCN2 and HCN4 protein expression determined by immunocytochemical techniques in sedentary and trained dog. Bar diagram on top left indicates that the relative density of dog cardiomyocytes with HCN4 immunolabelling obtained from the trained group (n=30 cells/6 dogs) is significantly increased compared to that measured in the sedentary group (n=30 cells/6 dogs). Original immunofluorescent images are shown on the left . Bottom panels indicate lack of effect of chronic training on the relative density of dog cardiomyocytes with HCN1 and HCN2 immunolabelling (n=30 – 30 cells/6 – 6 dogs for sedentary and trained groups, respectively). Figure 7–Source Data 1 Effect of chronic training on HCN4 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 2 Effect of chronic training on HCN1 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 3 Effect of chronic training on HCN2 protein expression determined by immunocytochemical technique in sedentary and trained dogs.

    Journal: bioRxiv

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibilty in a canine model of elite exercise

    doi: 10.1101/2022.07.13.499876

    Figure Lengend Snippet: Effect of chronic training on HCN1, KCN2 and HCN4 protein expression determined by immunocytochemical techniques in sedentary and trained dog. Bar diagram on top left indicates that the relative density of dog cardiomyocytes with HCN4 immunolabelling obtained from the trained group (n=30 cells/6 dogs) is significantly increased compared to that measured in the sedentary group (n=30 cells/6 dogs). Original immunofluorescent images are shown on the left . Bottom panels indicate lack of effect of chronic training on the relative density of dog cardiomyocytes with HCN1 and HCN2 immunolabelling (n=30 – 30 cells/6 – 6 dogs for sedentary and trained groups, respectively). Figure 7–Source Data 1 Effect of chronic training on HCN4 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 2 Effect of chronic training on HCN1 protein expression determined by immunocytochemical technique in sedentary and trained dogs. Figure 7–Source Data 3 Effect of chronic training on HCN2 protein expression determined by immunocytochemical technique in sedentary and trained dogs.

    Article Snippet: After the incubation period, cells were labelled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID:AB_2756744), anti-Kv4.3 (Alomone, #APC-017, RRID:AB_2040178), anti-HCN1 (Alomone, #APC-056, RRID:AB_2039900), anti-HCN2 (Alomone, #APC-030, RRID:AB_2313726) and anti-HCN4 (Alomone, #APC-052, RRID:AB_2039906) primary antibody diluted to 1:50.

    Techniques: Expressing