hcmv ad169 strain  (ATCC)


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    ATCC hcmv ad169 strain
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Ad169 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells"

    Article Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-8-85

    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain AD169 in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Figure Legend Snippet: Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain AD169 in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.

    Techniques Used: Activity Assay, Recombinant, Purification, Chromatography, Infection, Staining, Inhibition, Concentration Assay, Software

    hcmv ad169  (ATCC)


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    ATCC hcmv ad169
    Hcmv Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcmv strains ad169  (ATCC)


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    ATCC hcmv strains ad169
    Hcmv Strains Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ad169 hcmv strain  (ATCC)


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    ATCC ad169 hcmv strain
    Ad169 Hcmv Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcmv strain ad169 atcc vr 538d  (ATCC)


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    ATCC hcmv strain ad169 atcc vr 538d
    Hcmv Strain Ad169 Atcc Vr 538d, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcmv standard strain ad169  (ATCC)


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    ATCC hcmv standard strain ad169
    Hcmv Standard Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcmv standard strain ad169  (ATCC)


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    ATCC hcmv standard strain ad169
    Hcmv Standard Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcmv strain ad169  (ATCC)


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    ATCC hcmv strain ad169
    Hcmv Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcmv strain ad169  (ATCC)


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    ATCC hcmv strain ad169
    Hcmv Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcmv ad169 strain  (ATCC)


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    ATCC hcmv ad169 strain
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Ad169 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells"

    Article Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-8-85

    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain AD169 in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Figure Legend Snippet: Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain AD169 in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.

    Techniques Used: Activity Assay, Recombinant, Purification, Chromatography, Infection, Staining, Inhibition, Concentration Assay, Software

    hcmv strain ad169  (ATCC)


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    ATCC hcmv strain ad169
    Hcmv Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hcmv ad169 strain
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Ad169 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hcmv ad169
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hcmv strains ad169
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Strains Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ad169 hcmv strain
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Ad169 Hcmv Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hcmv strain ad169 atcc vr 538d
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Strain Ad169 Atcc Vr 538d, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hcmv standard strain ad169
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Standard Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hcmv strain ad169
    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain <t>AD169</t> in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.
    Hcmv Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcmv strain ad169/product/ATCC
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    Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain AD169 in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.

    Journal: BMC Biotechnology

    Article Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells

    doi: 10.1186/1472-6750-8-85

    Figure Lengend Snippet: Neutralizing activity of natural (■) and recombinant (○) purified 26A1 IgG . The 26A1 mAb was purified from serum-free supernatants by protein A chromatography. The neutralizing activity of HCMV infectivity was then tested either with the laboratory strain AD169 in HELF (panel A) or with the clinical isolate VR1814 in HUVEC (panel B). The effect of 26A1 IgG on HCMV infectivity was measured by staining the HCMV IEA by indirect immunoperoxidase. Five fields (HPF 20×) were counted per well from triplicate wells. Percentage of inhibition of viral infectivity was calculated using the following formula: [(means ± s.d. of IEA + nuclei of HCMV infected cells – means ± s.d. of IEA + nuclei of 26A1-treated HCMV infected cells/means ± s.d. of IEA + nuclei of HCMV infected cells) ×100]. Results represent the means ± s.d. of 3 independent experiments. Plaque reduction assay (panel C) shows the neutralizing activity of natural and recombinant 26A1 mAb against HCMV AD169 (1,000 pfu/rxn) in HELF. The values were plotted as a function of IgG concentration, and the concentrations of IgG required to inhibit viral infection by 50% (IC 50 ) were calculated by linear regression using GraphPad Prism (v. 4.0) software.

    Article Snippet: The effect on HCMV infectivity was determined by an HCMV microneutralization assay based on the evaluation of the ability of clone supernatants to interfere on the infectivity of HCMV AD169 strain (a laboratory strain from ATCC, cod.

    Techniques: Activity Assay, Recombinant, Purification, Chromatography, Infection, Staining, Inhibition, Concentration Assay, Software