Journal: Infectious Agents and Cancer

Article Title: Is human cytomegalovirus associated with breast cancer progression?

doi: 10.1186/1750-9378-8-12

Figure Lengend Snippet: Quality control for breast carcinomas and fibroadenomas. Multiplex PCR for GADPH. ( A ) Lines 1−9 and 19−26 breast carcinomas; 10−18 fibroadenomas. ( B ) Lines 27, 37−45 breast carcinomas; 19−30, 46 and 47 fibroadenomas. Base pairs (bp); control positive (+), HCMV strain AD-169 (ATCC® VR-583); control negative (−), DNA white blood cells from healthy individual (blood donor) and reaction mix without DNA.

Article Snippet: Positive control was done with HCMV strain AD-169 (ATCC VR-583).

Techniques: Multiplex Assay

Journal: Bioinformation

Article Title: Functional characterization of novel mutations in UL54 of ganciclovir resistant HCMV strain using structural analysis

doi:

Figure Lengend Snippet: The amplified products of region IV of the specimens along with others specimens detected positive to HCMV. Agarose Gel Electrophoretogram of PCR amplified product targeting Human cytomegalovirus UL54 gene of Region IV. Round II applied on Clinical Specimens. Some of the specimens positive for the region IV along with sample 2256 is shown. Lanes NC2: Negative Control - II round NC1: Negative Control - I round PC: Positive Control DNA extract from Human cytomegalovirus AD 169 MWM: Molecular weight marker 100 bp Ladder. Specimens positive for UL54 partial region: 2526, 4474, 4869, 1650, 4069

Article Snippet: DNA extracted from HCMV AD 169 (ATCC VR 538) was used as a positive control.

Techniques: Amplification, Agarose Gel Electrophoresis, Negative Control, Positive Control, Molecular Weight, Marker

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Inhibition activity of dendrimers against HCMV AD−169 . Plaque reduction assays were performed in MRC-5 cells for 6 days treatments. MRC-5 cells were pre-treated for 1 h at 37 ºC with increased concentrations of G1-S4, G2-S16 and G2-S24P PCDs (1, 5 and 10 µM) and infected with 60 PFU at 37 ºC. Plaques were counted after 6 days with a methylene blue dye. Percentage of infection is represented as the number of plaques in treatments in regard of the control of infection. CI, Control of Infection; NT, non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Inhibition, Activity Assay, Infection

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Representative images of HCMV AD - 169 plaques in treatments with G2-S16 dendrimer. a Optical and b confocal representative images of MRC-5 cells. Briefly, MRC-5 cells were plated and treated with G2-S16 dendrimer (10 µM) for 1 h and infected with 60 PFU for 2 h at 37 ºC. After 6 days incubation a MRC-5 cells were dyed with methylene blue and images were taken on a DMIL Leica microscope at 4X augment. b MRC-5 cells were treated with HCMVpp65 ALEXA FLUOR® 488 Antibody Conjugated for viral particles visualization, phalloidin ALEXA FLUOR® 555 Conjugated and DAPI for membrane and nucleus localization. NT: non-treated; CI: control of infection

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Infection, Incubation, Microscopy

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Activity of PCDs at cell membrane-virus attachment. Plaque reduction assays were performed in MRC-5 cells for 6 days treatments. MRC-5 cells were pre-cooled at 4 ºC and pre-treated for 1 h at 4 ºC with increased concentrations of G1-S4, G2-S16 and G2-S24P PCDs (1, 5 and 10 µM) and infected with 60 PFU at 4 ºC. Plaques were counted after 6 days with a methylene blue dye. Percentage of infection is represented as the number of plaques in treatments in regard of the control of HCMV AD−169 infection. CI: Control of Infection NT = non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Activity Assay, Infection

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Virucidal activity of G2-S16 or G2-S24P dendrimers against HCMV AD−169 . Plaque reduction assays were performed in MRC-5 cells for 6 days treatments. G2-S16 or G2-S24P dendrimer at 10 µM was incubated with 60 PFU for 2 h at 37 ºC. By several centrifugations at 12,000 RPM virus was pelleted, resuspended in fresh DMEM and added to MRC-5 cells for plaque reduction assays. Plaques were counted after 6 days with a methylene blue dye. Percentage of HCMV AD−169 infection is represented as the number of plaques in treatments in regard of the control of infection. Triton X-100 was used as a positive control of virus membrane disruption. CI: Control of Infection, NT: non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Activity Assay, Incubation, Infection, Positive Control

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Time of addition of G2-S16 or G2-S24P dendrimer and their combinations with ganciclovir. Plaque reduction assays were performed in MRC-5 cells for 6 days. MRC-5 cells were seeded, infected with 60 PFU and treated at different time points (0, 24, 48, 72 and 96 h post-infection) with either a ganciclovir, G2-S16 dendrimer and G2-S16 dendrimer + ganciclovir combination b ganciclovir, G2-S24P dendrimer, and G2-S24P + ganciclovir. Plaques were counted after 6 days with a methylene blue dye. Percentage of HCMV AD−169 infection is represented as the number of plaques in treatments in regard of the control of HCMV AD−169 infection. CI: Control of infection, NT: non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Infection