Journal: BMC Cancer

Article Title: Reduced repair of 8-hydroxyguanine in the human breast cancer cell line, HCC1937

doi: 10.1186/1471-2407-6-297

Figure Lengend Snippet: Expression of antioxidants in HCC1937 and MCF-7 relative to AG11134 . Western Blot analysis of SOD1, SOD2 and catalase showing their levels in the two breast cancer cell lines compared to the normal mammary epithelial cell line, AG11134. β-actin was used as a loading control. The gels are representatives of three independent analyses in each case.

Article Snippet: HCC1937 is a primary ductal breast carcinoma cell line that was purchased from American Type Culture Collection (ATCC Manassas, VA).

Techniques: Expressing, Western Blot

Journal: BMC Cancer

Article Title: RAD6 inhibition enhances paclitaxel sensitivity of triple negative breast cancer cells by aggravating mitotic spindle damage

doi: 10.1186/s12885-022-10119-z

Figure Lengend Snippet: Lack of association between BRCA1 status and centrosome number and paclitaxel (PTX) response. A TNBC cell lines were fixed and immunostained with anti-pericentrin antibody. Nuclei were counterstained with DAPI. Bar, 10 µm. B Centrosome number quantification. Approximately 75 cells in four to six fields were scored for centrosome number. Data are shown as mean ± S.E.M from at least 2 independent experiments. Bars, 10 µm. C PTX sensitivities of BRCA1 wild type MDA-MB-231, MDA-MB-468, and BRCA1 mutant SUM1315 and HCC1937 TNBC cell lines measured by MTT assays. Results are expressed as mean ± S.D. of three independent experiments

Article Snippet: BRCA1 wild type MDA-MB-468 and MDA-MB-231, and BRCA1 mutant HCC1937 TNBC cells were purchased from ATCC (Manassas, VA).

Techniques: Mutagenesis

Journal: BMC Cancer

Article Title: RAD6 inhibition enhances paclitaxel sensitivity of triple negative breast cancer cells by aggravating mitotic spindle damage

doi: 10.1186/s12885-022-10119-z

Figure Lengend Snippet: SMI#9 synergistically enhances paclitaxel (PTX) sensitivity in BRCA1 wild type and mutant TNBC cell lines . MDA-MB-468 ( A-C ) and HCC1937 ( D-F ) cells were treated with PTX alone at the indicated concentrations or along with 0.75 µM SMI#9 and proliferating cells measured by MTT assays. C, F Combination indices (CI) were calculated with the CompuSyn software where a CI > 1 indicates antagonism, CI < 1 indicates synergy, and CI = 1 indicates an additive effect. Results are expressed as mean ± S.E.M from three independent experiments. * P < 0.05, ** P < 0.005, **** P < 0.00005

Article Snippet: BRCA1 wild type MDA-MB-468 and MDA-MB-231, and BRCA1 mutant HCC1937 TNBC cells were purchased from ATCC (Manassas, VA).

Techniques: Mutagenesis, Software

Journal: BMC Cancer

Article Title: RAD6 inhibition enhances paclitaxel sensitivity of triple negative breast cancer cells by aggravating mitotic spindle damage

doi: 10.1186/s12885-022-10119-z

Figure Lengend Snippet: RAD6 inhibition induces multinucleation and giant nuclei formation . MDA-MB-468 ( A-C ) and HCC1937 ( D-F ) cells were treated with the indicated doses of paclitaxel (PTX) for 24 h, and 100 viable cells were replated as single cells in quadruplicates post PTX treatment. Colonies were allowed to form and were then either left untreated or treated with 0.75 µM SMI#9. A , D Colony forming efficiency. A ** P < 0.005 and one-way ANOVA, P = 0.022; D * P < 0.01, ** P < 0.005 and one-way ANOVA, P = 0.0002. B, E Quantitation of colonies containing giant or multinucleated abnormal cells. Results are expressed as mean ± S.E.M. of replicates. E One-way ANOVA, P = 0.038. C, F Representative phase contrast images (long arrow, multinucleated cells; short arrow, cell with giant nucleus). Scale bars, 20 μM

Article Snippet: BRCA1 wild type MDA-MB-468 and MDA-MB-231, and BRCA1 mutant HCC1937 TNBC cells were purchased from ATCC (Manassas, VA).

Techniques: Inhibition, Quantitation Assay

Journal: BMC Cancer

Article Title: RAD6 inhibition enhances paclitaxel sensitivity of triple negative breast cancer cells by aggravating mitotic spindle damage

doi: 10.1186/s12885-022-10119-z

Figure Lengend Snippet: Paclitaxel and SMI#9 decrease 1N3R TAU isoform and modulate cyclin B1 protein levels differently in paclitaxel-sensitive and intrinsically resistant TNBC cells . Western blot analysis of the indicated proteins in MDA-MB-468 ( A, B ) and HCC1937 ( C-E ) cells treated with PTX, SM#9 or SMI#9 + PTX at the indicated nanomolar concentrations. E Cyclin B1 analysis in MG132 treated HCC1937 cells. B, D Protein levels were normalized to β-actin using Image J software

Article Snippet: BRCA1 wild type MDA-MB-468 and MDA-MB-231, and BRCA1 mutant HCC1937 TNBC cells were purchased from ATCC (Manassas, VA).

Techniques: Western Blot, Software

Journal: BMC Cancer

Article Title: RAD6 inhibition enhances paclitaxel sensitivity of triple negative breast cancer cells by aggravating mitotic spindle damage

doi: 10.1186/s12885-022-10119-z

Figure Lengend Snippet: Paclitaxel and SMI#9 induce accumulation of tetraploid cells in S and G2/M phases . DNA content analyzed by flow cytometry after 24 and 48 h of treatment with PTX (5 nM for MDA-MB-468 and 10 nM for HCC1937 cells), SMI#9 (0.75 µM) or a combination of SMI#9 with the corresponding PTX dose. A, B MDA-MB-468 and ( C, D ) HCC1937 cells. B, D Percentage of cells in diploid and tetraploid G1, S and G2/M phases were estimated after analysis using Modfit software

Article Snippet: BRCA1 wild type MDA-MB-468 and MDA-MB-231, and BRCA1 mutant HCC1937 TNBC cells were purchased from ATCC (Manassas, VA).

Techniques: Flow Cytometry, Software

Journal: BMC Cancer

Article Title: RAD6 inhibition enhances paclitaxel sensitivity of triple negative breast cancer cells by aggravating mitotic spindle damage

doi: 10.1186/s12885-022-10119-z

Figure Lengend Snippet: SMI#9 induces monopolar mitotic spindles . MDA-MB-468 ( A, B ) and HCC1937 ( C, D ) TNBC cells were treated with 2.5 or 10 nM PTX, respectively, 0.75 µM SMI#9 or SMI#9 + PTX and analyzed for TAU (green) and α-tubulin (red) expression/localization by immunofluorescence staining. Bar, 10 µm. Enlarged images of α-tubulin stained mitotic spindles are shown. B, D Quantitation of total TAU positive cells and those with monopolar mitotic spindles. Results are expressed as mean ± S.E.M of replicates. B * P < 0.05, C * P < 0.01

Article Snippet: BRCA1 wild type MDA-MB-468 and MDA-MB-231, and BRCA1 mutant HCC1937 TNBC cells were purchased from ATCC (Manassas, VA).

Techniques: Expressing, Immunofluorescence, Staining, Quantitation Assay