Journal: BMC Cancer

Article Title: Reduced repair of 8-hydroxyguanine in the human breast cancer cell line, HCC1937

doi: 10.1186/1471-2407-6-297

Figure Lengend Snippet: Expression of antioxidants in HCC1937 and MCF-7 relative to AG11134 . Western Blot analysis of SOD1, SOD2 and catalase showing their levels in the two breast cancer cell lines compared to the normal mammary epithelial cell line, AG11134. β-actin was used as a loading control. The gels are representatives of three independent analyses in each case.

Article Snippet: HCC1937 is a primary ductal breast carcinoma cell line that was purchased from American Type Culture Collection (ATCC Manassas, VA).

Techniques: Expressing, Western Blot

Journal: Cancers

Article Title: Small Molecule RBI2 Disrupts Ribosome Biogenesis through Pre-rRNA Depletion

doi: 10.3390/cancers15133303

Figure Lengend Snippet: Pre-rRNA levels rapidly decreasing in cancer cell lines after treatment with RBI2. RT-qPCR was carried out in ( A ) MDA-MB-231, ( B ) HCC1937, and ( C ) A375 cancer cell lines to probe their sensitivity to treatment with RBI2. IC90 concentrations, as determined by cell growth, were used for all treatments. Lines represent linear data interpolation. n = 3 for all cell lines, and error bars represent standard deviation with respect to the mean.

Article Snippet: Alamar blue standard curves and cell viability assays were created and performed, respectively, for the MDA-MB-231 and HCC1937 cell lines (purchased from ATCC) exactly as previously described for A375 cell lines [ ].

Techniques: Quantitative RT-PCR, Standard Deviation

Journal: Cancers

Article Title: Small Molecule RBI2 Disrupts Ribosome Biogenesis through Pre-rRNA Depletion

doi: 10.3390/cancers15133303

Figure Lengend Snippet: Pol I occupancy is unaltered in cancer cell lines after RBI2 treatment. ( A ) MDA-MB-231, ( B ) HCC1937, and ( C ) A375 cell lines were tested with respect to Pol I occupancy over the different regions of the rDNA after 120 min of treatment with DMSO (control, grey bars) or [IC90] RBI2 (green bars). GAPDH was used as a negative control, as Pol I does not transcribe mRNA coding genes. n = 3; error bars represent standard deviation for the mean. Statistically significant differences ( p < 0.05) are noted with and asterisk (*). All differences between the treated and untreated samples were insignificant, with the exception of a small decrease in the promoter region in MDA-MB-231 cells.

Article Snippet: Alamar blue standard curves and cell viability assays were created and performed, respectively, for the MDA-MB-231 and HCC1937 cell lines (purchased from ATCC) exactly as previously described for A375 cell lines [ ].

Techniques: Negative Control, Standard Deviation

Journal: BioMed Research International

Article Title: RNA Sequencing Analysis Reveals Interactions between Breast Cancer or Melanoma Cells and the Tissue Microenvironment during Brain Metastasis

doi: 10.1155/2017/8032910

Figure Lengend Snippet: Xenograft models of brain metastasis and primary breast cancer. (a) For models of brain metastasis, human breast cancer (231-Luc, HER2-60, and HER2-90) or melanoma (MeWo, WM3734) cell lines (or PBS as a control) were injected into the left ventricle of female immunodeficient mice. For models of primary breast cancer, human 231-Luc or HCC1937 cells were orthotopically injected into mammary fat pads. (b) Hematoxylin-eosin staining of mouse brain tissue with metastases formed by 231-Luc, MeWo, or WM3734 cells. Arrows indicate human metastatic cancer cells. Scale bars, 200 μ m.

Article Snippet: Human breast cancer cell line HCC1937 cells were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium supplemented with 10% FBS.

Techniques: Injection, Staining

Journal: Molecular Cancer

Article Title: Transduction of E2F-1 TAT fusion proteins represses expression of hTERT in primary ductal breast carcinoma cell lines

doi: 10.1186/1476-4598-7-28

Figure Lengend Snippet: Immunocytochemistry images of HCC1937 cells . Immunocytochemistry images of HCC1937 cells transduced with Tat fusion proteins for 24 hours. (A) Cells treated with 2 μM E2F-1/TatHA. (B) Cells treated with 2 μM E132/TatHA. (C) Cells treated with 2 μM TatHA control peptide. Images were captured using the 40× objective on the Bio-Rad MRC 1024 Laser Scanning Confocal Microscope.

Article Snippet: Primary infiltrating ductal breast carcinoma cell lines HCC1937 (ATCC number: CRL-2336) and HCC1599 (ATCC number: CRL-2331) were obtained from the American Type Culture Collection (Manassas, VA).

Techniques: Immunocytochemistry, Transduction, Microscopy

Journal: Molecular Cancer

Article Title: Transduction of E2F-1 TAT fusion proteins represses expression of hTERT in primary ductal breast carcinoma cell lines

doi: 10.1186/1476-4598-7-28

Figure Lengend Snippet: Fold repression of hTERT gene expression in breast cancer cell lines- TatHA control . Fold changes in hTERT gene expression compared to TatHA control in breast cancer cell lines. HCC1937 and HCC1599 cells were treated for 24 hours with 2 μM Tat fusion proteins. RNA was isolated and RT-qPCR performed as described under Materials and Methods. The asterisks (*) indicate statistically significant fold changes in gene expression. A significant 3.5-fold repression of hTERT gene expression was observed in HCC1937 cells treated with E2F-1/TatHA proteins in comparison to treatment with TatHA control proteins (p < 0.0024). Repression of hTERT in HCC1937 cells treated with E132/TatHA proteins versus the TatHA control proteins was not statistically significant (p < 0.6484). A 3.3-fold repression of hTERT was observed in HCC1599 cells treated with E2F-1/TatHA proteins versus the TatHA control protein (p < 0.0001). hTERT gene expression was slightly increased (1.15-fold increase) in HCC1599 cells treated with E132/TatHA proteins versus the TatHA control proteins (p < 0.0102). The results are means +/- SEM of three separate experiments (HCC1937, n = 17; HCC1599, n = 18).

Article Snippet: Primary infiltrating ductal breast carcinoma cell lines HCC1937 (ATCC number: CRL-2336) and HCC1599 (ATCC number: CRL-2331) were obtained from the American Type Culture Collection (Manassas, VA).

Techniques: Expressing, Isolation, Quantitative RT-PCR

Journal: Theranostics

Article Title: A nano-liposome formulation of the PARP inhibitor Talazoparib enhances treatment efficacy and modulates immune cell populations in mammary tumors of BRCA-deficient mice

doi: 10.7150/thno.36281

Figure Lengend Snippet: Pharmacokinetics and pharmacodynamics of NanoTLZ. A. Mice bearing orthotopic BRCA-mutated human HCC1937 xenografts were injected with 1 mg/kg NanoTalazoparib (i.v.). Plasma drug concentrations were detected via HPLC. A two-compartment model was fit to the plasma data using PKSolver. B. Tumor PAR levels were detected via ELISA. ****, p<0.0001 for all time points compared to control C. Brca1 Co/Co ;MMTV-Cre;p53 +/- mice (N=4) were injected with a single dose of Cy5-encapsulated nanoparticles, and the fluorescent signal was detected using a IVIS spectrum imaging system 24 hrs after injection. Major organs were dissected after in vivo imaging (left) and biodistribution of nanoparticles in these organs was detected (right). Blue arrows point to tumors. Representative images are shown. D. W780 cells were treated with 5% empty nanoparticles (vehicle) or Cy5-encapsulated nanoparticles for 1-2 hrs. Fluorescent Cy5 signal was detected with a fluorescence microscope. 200x magnification.

Article Snippet: An orthotopic xenograft model of human BRCA-mutated breast cancer was established via injection of 5x10 6 HCC1937 cells (ATCC, Manassas, VA; cells were cultured in RPMI1640 with 10% FBS and 1% Pen/Strep) in the mammary fat pad of female NCr-nu/nu mice.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Imaging, In Vivo Imaging, Fluorescence, Microscopy

Journal: International Journal of Biological Sciences

Article Title: Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1 -associated Mammary Tumor Formation

doi: 10.7150/ijbs.28142

Figure Lengend Snippet: Estrogen induces proliferation of BRCA1-deficient mammary gland epithelial cells. (A) Protein expression patterns in MCF7 cells treated with different concentration of E2; β-actin was detected as a loading control. MCF7 cells were transfected with control siRNA or siRNA targeting BRCA1 , and treated with the indicated concentrations of E2 (B) for the indicated durations (C), after which survival was estimated using the MTT assay. The numbers represent mean values ± SD. (D) Whole-mount and (E) H&E staining of abdominal mammary glands from 2-month-old Brca1 co/co MMTV-cre and age-matched wild-type ( Brca1 co/co ) female mice treated with vehicle or E2. The panels at right are magnifications of the boxed areas in the adjacent panels (E). Scale bars: 200 μm.

Article Snippet: MCF7 cells (wild-type BRCA1 ) and BRCA1 -mutant HCC1937 cells were obtained from the American Type Culture Collection.

Techniques: Expressing, Concentration Assay, Transfection, MTT Assay, Staining

Journal: International Journal of Biological Sciences

Article Title: Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1 -associated Mammary Tumor Formation

doi: 10.7150/ijbs.28142

Figure Lengend Snippet: Estrogen reduces the ability to sense DNA damage and alters G1/S phase transition in BRCA1 -knockdown MCF7 cells. (A) MCF7 cells transfected with control siRNA or siRNA targeting BRCA1 were irradiated (0.3 Gy) in the absence or presence of E2 (100 nM), then immunostained for γ-H2AX foci (red) and counterstained with DAPI to detect nuclei (blue). (B). Foci numbers and signal accumulation per cell are shown in the histogram (* P < 0.05, ** P < 0.01). (C) MCF7 cells transfected with control siRNA or siRNA targeting BRCA1 were irradiated in the absence or presence of E2 (100 nM), and DNA breakage was measured using Komet software (Andor Technology). (D) Olive tail moments of comet assays were calculated from at least 30 cells for each condition. (E) Representative histograms showing the DNA content of MCF7 cells. MCF7 cells transfected with control siRNA or siRNA targeting BRCA1 were treated with E2 (100 nM) for 1 day and analyzed by flow cytometry with propidium iodide staining. (F) Percentages of cells in each phase of the cell cycle are shown.

Article Snippet: MCF7 cells (wild-type BRCA1 ) and BRCA1 -mutant HCC1937 cells were obtained from the American Type Culture Collection.

Techniques: Sublimation, Transfection, Irradiation, Software, Flow Cytometry, Staining

Journal: International Journal of Biological Sciences

Article Title: Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1 -associated Mammary Tumor Formation

doi: 10.7150/ijbs.28142

Figure Lengend Snippet: Estrogen increases the rigidity of mammary glands in Brca1 -mutant mice. (A) Branch software (ver. 1.1) was used to estimate the total length (open) and branch numbers (filled) of ducts between the lymph node and end tip in mammary glands of 12-month-old Brca1 co/co MMTV-Cre mice in the absence or presence of E2 (1.7 mg) for 90 days. (B) The density of the mammary gland was significantly increased in E2-treated mice compared with control mice (** P < 0.01). (C) Representative whole-mount stainings of mammary glands from 12-month-old Brca1 co/co MMTV-Cre mice in the absence or presence of E2 for 90 days. (D) Histological analysis of mammary glands in the absence or presence of E2-beads. (E) Levels of PCNA and cyclin D1 in dense mammary glands were examined in the absence or presence of E2-beads by immunohistochemical analysis. Scale bars: 100 μm.

Article Snippet: MCF7 cells (wild-type BRCA1 ) and BRCA1 -mutant HCC1937 cells were obtained from the American Type Culture Collection.

Techniques: Mutagenesis, Software, Immunohistochemical staining

Journal: International Journal of Biological Sciences

Article Title: Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1 -associated Mammary Tumor Formation

doi: 10.7150/ijbs.28142

Figure Lengend Snippet: Inhibition of estrogen signaling by fulvestrant treatment reduces mammary tumor formation in Brca1 co/co MMTV-cre mice. (A) Kaplan-Meier curves showing tumor-free survival of Brca1 co/co MMTV-cre mice. Nine-month-old Brca1 co/co MMTV-cre mice were treated with vehicle (n = 14) or fulvestrant (n = 14) every other week for 5 months. Nine of 14 (64%) vehicle-treated mice and five of 14 (36%) fulvestrant-treated mice spontaneously developed palpable mammary tumors during this period. (B) Protein expression patterns and (C) histological analysis of tumors from vehicle- and fulvestrant-treated mice. Areas highlighted by dotted lines represent necrotic regions. Scale bars: 100 μm.

Article Snippet: MCF7 cells (wild-type BRCA1 ) and BRCA1 -mutant HCC1937 cells were obtained from the American Type Culture Collection.

Techniques: Inhibition, Expressing

Journal: International Journal of Biological Sciences

Article Title: Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1 -associated Mammary Tumor Formation

doi: 10.7150/ijbs.28142

Figure Lengend Snippet: Fulvestrant treatment decreases the rigidity of mammary glands in Brca1 -mutant mice. (A) Branch software (ver. 1.1) was used to estimate the total length (circles) and branch numbers (triangles) of ducts between the lymph node and end tip in mammary glands of 14-month-old non-tumor-bearing mice. (B) The density of the mammary gland was significantly lower in fulvestrant-treated mice than in vehicle-treated mice (* P < 0.05, ** P < 0.01). (C) Representative whole-mount stainings of non-tumor-bearing mammary glands from 14-month-old Brca1 co/co MMTV-Cre mice treated with vehicle or fulvestrant for 5 months. (D) Histological analysis of mammary glands from control and fulvestrant-treated mice. Areas highlighted by boxes (left panels) were further analyzed by H&E staining, immunohistochemistry, and trichrome staining.

Article Snippet: MCF7 cells (wild-type BRCA1 ) and BRCA1 -mutant HCC1937 cells were obtained from the American Type Culture Collection.

Techniques: Mutagenesis, Software, Staining, Immunohistochemistry

Journal: International Journal of Biological Sciences

Article Title: Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1 -associated Mammary Tumor Formation

doi: 10.7150/ijbs.28142

Figure Lengend Snippet: Inhibition of estrogen signaling fails to suppress the growth of Brca1 -mutant breast tumors. (A) Protein expression patterns in BRCA1-deficient HCC1937 cells treated with E2 (100 nM); β-actin was detected as a loading control. (B) HCC1937 cells were treated with the indicated concentrations of FBS, with or without E2 (100 nM), and survival was estimated using the MTT assay. (C) Overview of the allograft model and fulvestrant treatments. Eighteen spontaneously developed mammary tumors were collected from Brca1 co/co MMTV-Cre mice and transplanted into nude mice. Corresponding tumors grown under mock conditions were compared with those treated with fulvestrant. After tumors in any mouse implanted with the same original tumor reached 3 cm 3 , all mice implanted with that tumor were sacrificed and examined. (D) Graph shows calculated RTVs (RTV of treated tumor/RTV of control tumor X 100) for tumors treated with fulvestrant. (E) Analysis of fulvestrant response-associated biomarkers. Heat map shows selected downregulated and upregulated genes according to the responsiveness to fulvestrant (| R | > 0.6, P < 0.05). Tumor samples are sorted with respect to the fulvestrant response to highlight the correlation between response and gene expression. Highly correlated genes are ranked high in the heat map. Top-ranked genes only were selected for presentation (see Supplementary Tables and 2 for the full gene list). (F and G) Functional GO enrichment analysis of genes that were positively correlated (F) and negatively correlated (G) with fulvestrant responsiveness. A node is an enriched GO term (Bonferroni corrected P < 0.05), and two associated terms connected by a line share many responsive genes (Cohen's kappa score > 0.4). The node label with the highest significance among associated terms is colored.

Article Snippet: MCF7 cells (wild-type BRCA1 ) and BRCA1 -mutant HCC1937 cells were obtained from the American Type Culture Collection.

Techniques: Inhibition, Mutagenesis, Expressing, MTT Assay, Functional Assay