hcaecs  (Cell Applications Inc)

Journal: bioRxiv

Article Title: Comprehensive multi-omics study of the molecular perturbations induced by simulated diabetes on coronary artery endothelial cells

doi: 10.1101/2021.01.06.425584

Figure Lengend Snippet: Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.

Article Snippet: For selected experiments (binucleation analysis), HCAEC (55 years old Caucasian male, history of T2DM for > 5 years) were purchased from Cell Applications, Inc. and subjected to the same conditions as BCAEC but using MesoEndo Growth Medium (Cell Applications, Inc.) to induce proliferation.

Techniques: Immunofluorescence, Staining, Fluorescence, Imaging, Software