Journal: Biomedicines

Article Title: Identification of Key Genes and Pathways in Genotoxic Stress Induced Endothelial Dysfunction: Results of Whole Transcriptome Sequencing

doi: 10.3390/biomedicines10092067

Figure Lengend Snippet: Heatmaps of differentially expressed genes (DEGs) identified in ( A ) Human Coronary Artery Endothelial Cells (HCAEC) and ( B ) Human Internal Thoracic Endothelial Cells (HITAEC) exposed to mitomycin C (MMC) in comparison with the non-exposed control (only those having FDR p -value < 0.05 are presented in the heatmaps). Top panel—the results of hierarchical sample clustering (C1, C2, C3, and C4—the control group, T1, T2, T3, and T4—the treatment group). Left panel—the results of hierarchical DEGs clustering (DEGs labeling based on Ensembl annotation). Bottom panel—the absolute fold change.

Article Snippet: Cryovials containing 5 × 10 5 primary Human Coronary Artery Endothelial Cells (HCAEC) and Human Internal Thoracic Endothelial Cells (HITAEC) (Cell Applications Inc., San Diego, CA, USA) were thawed; cells were transferred into fibronectin-coated 75 cm 2 culture flasks containing 15 mL of Human MesoEndo Cell Growth Medium (Cell Applications Inc., San Diego, CA, USA) and cultured in the MCO-18AIC CO 2 Incubator (Sanyo Electric Co., Ltd., Japan) at 37 °C, 5% CO 2 in humidified conditions.

Techniques: Labeling

Journal: Biomedicines

Article Title: Identification of Key Genes and Pathways in Genotoxic Stress Induced Endothelial Dysfunction: Results of Whole Transcriptome Sequencing

doi: 10.3390/biomedicines10092067

Figure Lengend Snippet: The differentially expressed genes (DEGs) identified in Human Coronary Artery Endothelial Cells (HCAEC) and Human Internal Thoracic Endothelial Cells (HITAEC) exposed to mitomycin C (MMC) in comparison with the non-exposed control after applying the cut-off criterion (FDR p -value < 0.05, absolute fold change ≥ 2).

Article Snippet: Cryovials containing 5 × 10 5 primary Human Coronary Artery Endothelial Cells (HCAEC) and Human Internal Thoracic Endothelial Cells (HITAEC) (Cell Applications Inc., San Diego, CA, USA) were thawed; cells were transferred into fibronectin-coated 75 cm 2 culture flasks containing 15 mL of Human MesoEndo Cell Growth Medium (Cell Applications Inc., San Diego, CA, USA) and cultured in the MCO-18AIC CO 2 Incubator (Sanyo Electric Co., Ltd., Japan) at 37 °C, 5% CO 2 in humidified conditions.

Techniques:

Journal: Biomedicines

Article Title: Identification of Key Genes and Pathways in Genotoxic Stress Induced Endothelial Dysfunction: Results of Whole Transcriptome Sequencing

doi: 10.3390/biomedicines10092067

Figure Lengend Snippet: Quantity of differentially expressed genes (DEGs) identified in Human Coronary Artery Endothelial Cells (HCAEC) exposed to mitomycin C (MMC) in comparison with the non-exposed control in functional groups of biological processes according to Gene Ontology enrichment analysis.

Article Snippet: Cryovials containing 5 × 10 5 primary Human Coronary Artery Endothelial Cells (HCAEC) and Human Internal Thoracic Endothelial Cells (HITAEC) (Cell Applications Inc., San Diego, CA, USA) were thawed; cells were transferred into fibronectin-coated 75 cm 2 culture flasks containing 15 mL of Human MesoEndo Cell Growth Medium (Cell Applications Inc., San Diego, CA, USA) and cultured in the MCO-18AIC CO 2 Incubator (Sanyo Electric Co., Ltd., Japan) at 37 °C, 5% CO 2 in humidified conditions.

Techniques: Functional Assay

Journal: Biomedicines

Article Title: Identification of Key Genes and Pathways in Genotoxic Stress Induced Endothelial Dysfunction: Results of Whole Transcriptome Sequencing

doi: 10.3390/biomedicines10092067

Figure Lengend Snippet: Distribution of differentially expressed genes (DEGs) identified in Human Coronary Artery Endothelial Cells (HCAEC) exposed to mitomycin C (MMC) in comparison with the non-exposed control between functional groups of biological processes according to Gene Ontology enrichment analysis.

Article Snippet: Cryovials containing 5 × 10 5 primary Human Coronary Artery Endothelial Cells (HCAEC) and Human Internal Thoracic Endothelial Cells (HITAEC) (Cell Applications Inc., San Diego, CA, USA) were thawed; cells were transferred into fibronectin-coated 75 cm 2 culture flasks containing 15 mL of Human MesoEndo Cell Growth Medium (Cell Applications Inc., San Diego, CA, USA) and cultured in the MCO-18AIC CO 2 Incubator (Sanyo Electric Co., Ltd., Japan) at 37 °C, 5% CO 2 in humidified conditions.

Techniques: Functional Assay, Transduction, Cell Surface Receptor Assay, Migration