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Cell Applications Inc hcaecs
Hcaecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcaecs/product/Cell Applications Inc
Average 86 stars, based on 1 article reviews
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hcaecs - by Bioz Stars, 2022-07
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    Cell Applications Inc hcaec
    Increased cellular <t>binucleation</t> by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells <t>(HCAEC).</t> (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.
    Hcaec, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcaec/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcaec - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    Cell Applications Inc hcaecs
    Increased cellular <t>binucleation</t> by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells <t>(HCAEC).</t> (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.
    Hcaecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcaecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcaecs - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    Cell Applications Inc human coronary artery endothelial cells hcaecs
    Increased cellular <t>binucleation</t> by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells <t>(HCAEC).</t> (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.
    Human Coronary Artery Endothelial Cells Hcaecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronary artery endothelial cells hcaecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronary artery endothelial cells hcaecs - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

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    Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.

    Journal: bioRxiv

    Article Title: Comprehensive multi-omics study of the molecular perturbations induced by simulated diabetes on coronary artery endothelial cells

    doi: 10.1101/2021.01.06.425584

    Figure Lengend Snippet: Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.

    Article Snippet: For selected experiments (binucleation analysis), HCAEC (55 years old Caucasian male, history of T2DM for > 5 years) were purchased from Cell Applications, Inc. and subjected to the same conditions as BCAEC but using MesoEndo Growth Medium (Cell Applications, Inc.) to induce proliferation.

    Techniques: Immunofluorescence, Staining, Fluorescence, Imaging, Software