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Cell Applications Inc hcaec
Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells <t>(HCAEC).</t> ( A ) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 µg/mL in HBSS). White arrows indicate binucleated cells. ( B ) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG + HI) versus control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS FLoid Cell Imaging Station with a fixed × 20 air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG normal glucose, HG high glucose, HI high insulin.
Hcaec, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcaec/product/Cell Applications Inc
Average 86 stars, based on 4 article reviews
Price from $9.99 to $1999.99
hcaec - by Bioz Stars, 2022-08
86/100 stars

Images

1) Product Images from "Multi-omics study identifies novel signatures of DNA/RNA, amino acid, peptide, and lipid metabolism by simulated diabetes on coronary endothelial cells"

Article Title: Multi-omics study identifies novel signatures of DNA/RNA, amino acid, peptide, and lipid metabolism by simulated diabetes on coronary endothelial cells

Journal: Scientific Reports

doi: 10.1038/s41598-022-16300-5

Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). ( A ) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 µg/mL in HBSS). White arrows indicate binucleated cells. ( B ) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG + HI) versus control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS FLoid Cell Imaging Station with a fixed × 20 air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG normal glucose, HG high glucose, HI high insulin.
Figure Legend Snippet: Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). ( A ) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 µg/mL in HBSS). White arrows indicate binucleated cells. ( B ) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG + HI) versus control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS FLoid Cell Imaging Station with a fixed × 20 air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG normal glucose, HG high glucose, HI high insulin.

Techniques Used: Immunofluorescence, Staining, Fluorescence, Imaging, Software

2) Product Images from "Comprehensive multi-omics study of the molecular perturbations induced by simulated diabetes on coronary artery endothelial cells"

Article Title: Comprehensive multi-omics study of the molecular perturbations induced by simulated diabetes on coronary artery endothelial cells

Journal: bioRxiv

doi: 10.1101/2021.01.06.425584

Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.
Figure Legend Snippet: Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.

Techniques Used: Immunofluorescence, Staining, Fluorescence, Imaging, Software

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    Cell Applications Inc human coronary artery endothelial cells hcaecs
    Exercise increases ABCA1‐mediated cholesterol efflux and improves the anti‐inflammatory properties of HDLs. ABCA1‐specific cholesterol efflux at baseline and after moderate‐ and high‐intensity exercise. Chinese hamster ovary <t>cells</t> with tetracycline‐inducible expression of <t>human</t> ABCA1 were loaded with 3 H‐cholesterol and incubated for 4 hours in the absence or presence of apoB‐depleted serum as described in the Methods. The percentage of efflux was calculated as radioactivity in the medium relative to the total radioactivity (cells+medium). ABCA1‐specific cholesterol efflux was calculated as the difference in efflux between cells incubated with and without tetracycline and normalized to a standard sample of apoB‐depleted serum (n=51) ( A ). ICAM‐1 and VCAM‐1 expression in TNF‐α activated <t>HCAECs</t> at baseline and after moderate‐ and high‐intensity ( B and C ). HCAECs were preincubated for 16 hours with ultracentrifugally isolated HDLs at baseline and after moderate‐ and high‐intensity exercise, then activated for 5 hours with TNF‐α. Expression of ICAM‐1 (n=19) ( B ) and VCAM‐1 (n=19) ( C ) was quantified by flow cytometry. Data represent the mean±SEM of triplicate measurements. Repeated measures ANOVA was conducted with Bonferroni post hoc pairwise comparison testing for multiple comparisons. *** P
    Human Coronary Artery Endothelial Cells Hcaecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronary artery endothelial cells hcaecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronary artery endothelial cells hcaecs - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    86
    Cell Applications Inc hcaec
    Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells <t>(HCAEC).</t> ( A ) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 µg/mL in HBSS). White arrows indicate binucleated cells. ( B ) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG + HI) versus control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS FLoid Cell Imaging Station with a fixed × 20 air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG normal glucose, HG high glucose, HI high insulin.
    Hcaec, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcaec/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcaec - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    86
    Cell Applications Inc hcaecs
    Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells <t>(HCAEC).</t> ( A ) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 µg/mL in HBSS). White arrows indicate binucleated cells. ( B ) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG + HI) versus control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS FLoid Cell Imaging Station with a fixed × 20 air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG normal glucose, HG high glucose, HI high insulin.
    Hcaecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcaecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcaecs - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    Image Search Results


    Exercise increases ABCA1‐mediated cholesterol efflux and improves the anti‐inflammatory properties of HDLs. ABCA1‐specific cholesterol efflux at baseline and after moderate‐ and high‐intensity exercise. Chinese hamster ovary cells with tetracycline‐inducible expression of human ABCA1 were loaded with 3 H‐cholesterol and incubated for 4 hours in the absence or presence of apoB‐depleted serum as described in the Methods. The percentage of efflux was calculated as radioactivity in the medium relative to the total radioactivity (cells+medium). ABCA1‐specific cholesterol efflux was calculated as the difference in efflux between cells incubated with and without tetracycline and normalized to a standard sample of apoB‐depleted serum (n=51) ( A ). ICAM‐1 and VCAM‐1 expression in TNF‐α activated HCAECs at baseline and after moderate‐ and high‐intensity ( B and C ). HCAECs were preincubated for 16 hours with ultracentrifugally isolated HDLs at baseline and after moderate‐ and high‐intensity exercise, then activated for 5 hours with TNF‐α. Expression of ICAM‐1 (n=19) ( B ) and VCAM‐1 (n=19) ( C ) was quantified by flow cytometry. Data represent the mean±SEM of triplicate measurements. Repeated measures ANOVA was conducted with Bonferroni post hoc pairwise comparison testing for multiple comparisons. *** P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Moderate‐ and High‐Intensity Exercise Improves Lipoprotein Profile and Cholesterol Efflux Capacity in Healthy Young Men

    doi: 10.1161/JAHA.121.023386

    Figure Lengend Snippet: Exercise increases ABCA1‐mediated cholesterol efflux and improves the anti‐inflammatory properties of HDLs. ABCA1‐specific cholesterol efflux at baseline and after moderate‐ and high‐intensity exercise. Chinese hamster ovary cells with tetracycline‐inducible expression of human ABCA1 were loaded with 3 H‐cholesterol and incubated for 4 hours in the absence or presence of apoB‐depleted serum as described in the Methods. The percentage of efflux was calculated as radioactivity in the medium relative to the total radioactivity (cells+medium). ABCA1‐specific cholesterol efflux was calculated as the difference in efflux between cells incubated with and without tetracycline and normalized to a standard sample of apoB‐depleted serum (n=51) ( A ). ICAM‐1 and VCAM‐1 expression in TNF‐α activated HCAECs at baseline and after moderate‐ and high‐intensity ( B and C ). HCAECs were preincubated for 16 hours with ultracentrifugally isolated HDLs at baseline and after moderate‐ and high‐intensity exercise, then activated for 5 hours with TNF‐α. Expression of ICAM‐1 (n=19) ( B ) and VCAM‐1 (n=19) ( C ) was quantified by flow cytometry. Data represent the mean±SEM of triplicate measurements. Repeated measures ANOVA was conducted with Bonferroni post hoc pairwise comparison testing for multiple comparisons. *** P

    Article Snippet: Human coronary artery endothelial cells (HCAECs) (Cell Applications Inc., San Diego CA) were grown to 90% confluence in 5% CO2 in Meso Endo Growth Medium (Cell Applications) and seeded onto 24‐well tissue culture plates (5×104 cells/well) in Meso Endo Growth Medium (500 µL).

    Techniques: Expressing, Incubation, Radioactivity, Isolation, Flow Cytometry

    Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). ( A ) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 µg/mL in HBSS). White arrows indicate binucleated cells. ( B ) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG + HI) versus control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS FLoid Cell Imaging Station with a fixed × 20 air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG normal glucose, HG high glucose, HI high insulin.

    Journal: Scientific Reports

    Article Title: Multi-omics study identifies novel signatures of DNA/RNA, amino acid, peptide, and lipid metabolism by simulated diabetes on coronary endothelial cells

    doi: 10.1038/s41598-022-16300-5

    Figure Lengend Snippet: Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). ( A ) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 µg/mL in HBSS). White arrows indicate binucleated cells. ( B ) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG + HI) versus control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS FLoid Cell Imaging Station with a fixed × 20 air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG normal glucose, HG high glucose, HI high insulin.

    Article Snippet: For selected experiments (binucleation analysis), HCAEC (55 years old Caucasian male, history of T2DM for > 5 years) were purchased from Cell Applications, Inc. and subjected to the same conditions as BCAEC but using MesoEndo Growth Medium (Cell Applications, Inc.) to induce proliferation.

    Techniques: Immunofluorescence, Staining, Fluorescence, Imaging, Software

    Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.

    Journal: bioRxiv

    Article Title: Comprehensive multi-omics study of the molecular perturbations induced by simulated diabetes on coronary artery endothelial cells

    doi: 10.1101/2021.01.06.425584

    Figure Lengend Snippet: Increased cellular binucleation by simulated diabetes in bovine coronary artery endothelial cells (BCAEC) and human coronary artery endothelial cells (HCAEC). (A) Representative immunofluorescence micrographs showing the localization of the von-Willebrand factor (vWf, 1:400, 3% BSA in PBS) in fixed and permeabilized cells. The nuclei were stained using the dye Hoechst 33258 (2 μg/ml in HBSS). White arrows indicate binucleated cells. (B) Quantification of binucleated cells in HCAEC and BCAEC under simulated diabetes (HG+HI) vs. control (NG) group. Fluorescence images were taken in at least three random fields per condition using an EVOS ® FLoid ® Cell Imaging Station with a fixed 20x air objective. Image analysis was performed by ImageJ software (version 2.0.0). Abbreviations: NG, normal glucose; HG, high glucose; HI, high insulin.

    Article Snippet: For selected experiments (binucleation analysis), HCAEC (55 years old Caucasian male, history of T2DM for > 5 years) were purchased from Cell Applications, Inc. and subjected to the same conditions as BCAEC but using MesoEndo Growth Medium (Cell Applications, Inc.) to induce proliferation.

    Techniques: Immunofluorescence, Staining, Fluorescence, Imaging, Software