hbv dna pcr fragment  (Roche)


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    Structured Review

    Roche hbv dna pcr fragment
    ( a ) Schematic representation of the intramolecular ligation technique to obtain the preCore and YMDD Pol motif close to each other. ( 1 ) The circle in a continuous line represents the complete <t>HBV</t> genome (3200 bp). The semicircles in discontinuous lines represent the first (first <t>PCR,</t> 2700 bp) and the nested (2194 bp for genotype A2 sequences and 2155 bp for genotype D sequences) PCR products. ( 2 ) The product obtained after intramolecular ligation and primers used to sequence the circular molecule ( Table 2 ) are indicated in the figure. ( b ) Agarose gel obtained after intramolecular digestion. Two markers (M-I and M-II) were loaded in the gel; band lengths are shown at the left side of the image. Two samples (S1 and S2) were loaded and four bands were detected; band lengths are indicated at the right side of the image. A band with an apparent size of 1700 bp was the circular molecule, with the preCore and YMDD Pol motif close to each other. Band B was the nested PCR product without ligation, and band D were dimers of nested PCR products without ligation. Band C corresponded to the ligated dimers. B , C and D bands were not used, because the main interest was to analyze regions from the same genome. ( c ) Sequence of the circular construct including the preCore region, YMDD polymerase motif (in box), and HindIII sequence. PreCore (1, 2, 14, 15, 28 and 29) and Core ( 1 ) codons analyzed in the text are also enclosed in boxes.
    Hbv Dna Pcr Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    hbv dna pcr fragment - by Bioz Stars, 2020-09
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    Related Products / Commonly Used Together

    t4 dna ligase

    Images

    1) Product Images from "Ultra-deep pyrosequencing analysis of the hepatitis B virus preCore region and main catalytic motif of the viral polymerase in the same viral genome"

    Article Title: Ultra-deep pyrosequencing analysis of the hepatitis B virus preCore region and main catalytic motif of the viral polymerase in the same viral genome

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkr451

    ( a ) Schematic representation of the intramolecular ligation technique to obtain the preCore and YMDD Pol motif close to each other. ( 1 ) The circle in a continuous line represents the complete HBV genome (3200 bp). The semicircles in discontinuous lines represent the first (first PCR, 2700 bp) and the nested (2194 bp for genotype A2 sequences and 2155 bp for genotype D sequences) PCR products. ( 2 ) The product obtained after intramolecular ligation and primers used to sequence the circular molecule ( Table 2 ) are indicated in the figure. ( b ) Agarose gel obtained after intramolecular digestion. Two markers (M-I and M-II) were loaded in the gel; band lengths are shown at the left side of the image. Two samples (S1 and S2) were loaded and four bands were detected; band lengths are indicated at the right side of the image. A band with an apparent size of 1700 bp was the circular molecule, with the preCore and YMDD Pol motif close to each other. Band B was the nested PCR product without ligation, and band D were dimers of nested PCR products without ligation. Band C corresponded to the ligated dimers. B , C and D bands were not used, because the main interest was to analyze regions from the same genome. ( c ) Sequence of the circular construct including the preCore region, YMDD polymerase motif (in box), and HindIII sequence. PreCore (1, 2, 14, 15, 28 and 29) and Core ( 1 ) codons analyzed in the text are also enclosed in boxes.
    Figure Legend Snippet: ( a ) Schematic representation of the intramolecular ligation technique to obtain the preCore and YMDD Pol motif close to each other. ( 1 ) The circle in a continuous line represents the complete HBV genome (3200 bp). The semicircles in discontinuous lines represent the first (first PCR, 2700 bp) and the nested (2194 bp for genotype A2 sequences and 2155 bp for genotype D sequences) PCR products. ( 2 ) The product obtained after intramolecular ligation and primers used to sequence the circular molecule ( Table 2 ) are indicated in the figure. ( b ) Agarose gel obtained after intramolecular digestion. Two markers (M-I and M-II) were loaded in the gel; band lengths are shown at the left side of the image. Two samples (S1 and S2) were loaded and four bands were detected; band lengths are indicated at the right side of the image. A band with an apparent size of 1700 bp was the circular molecule, with the preCore and YMDD Pol motif close to each other. Band B was the nested PCR product without ligation, and band D were dimers of nested PCR products without ligation. Band C corresponded to the ligated dimers. B , C and D bands were not used, because the main interest was to analyze regions from the same genome. ( c ) Sequence of the circular construct including the preCore region, YMDD polymerase motif (in box), and HindIII sequence. PreCore (1, 2, 14, 15, 28 and 29) and Core ( 1 ) codons analyzed in the text are also enclosed in boxes.

    Techniques Used: Ligation, Polymerase Chain Reaction, Sequencing, Agarose Gel Electrophoresis, Nested PCR, Construct

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    Roche hbv dna pcr fragment
    ( a ) Schematic representation of the intramolecular ligation technique to obtain the preCore and YMDD Pol motif close to each other. ( 1 ) The circle in a continuous line represents the complete <t>HBV</t> genome (3200 bp). The semicircles in discontinuous lines represent the first (first <t>PCR,</t> 2700 bp) and the nested (2194 bp for genotype A2 sequences and 2155 bp for genotype D sequences) PCR products. ( 2 ) The product obtained after intramolecular ligation and primers used to sequence the circular molecule ( Table 2 ) are indicated in the figure. ( b ) Agarose gel obtained after intramolecular digestion. Two markers (M-I and M-II) were loaded in the gel; band lengths are shown at the left side of the image. Two samples (S1 and S2) were loaded and four bands were detected; band lengths are indicated at the right side of the image. A band with an apparent size of 1700 bp was the circular molecule, with the preCore and YMDD Pol motif close to each other. Band B was the nested PCR product without ligation, and band D were dimers of nested PCR products without ligation. Band C corresponded to the ligated dimers. B , C and D bands were not used, because the main interest was to analyze regions from the same genome. ( c ) Sequence of the circular construct including the preCore region, YMDD polymerase motif (in box), and HindIII sequence. PreCore (1, 2, 14, 15, 28 and 29) and Core ( 1 ) codons analyzed in the text are also enclosed in boxes.
    Hbv Dna Pcr Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbv dna pcr fragment/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbv dna pcr fragment - by Bioz Stars, 2020-09
    85/100 stars
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    91
    Roche full length hbv dna fragment
    Susceptibilities to TDF of ETV‐resistant clones identified in this study. <t>HBV</t> molecular clones derived from patients A and B were transfected into HepG2 cells, and TDF was administered to the transfected cells at the indicated concentrations. (A, B) HBV replication and its reduction by TDF administration were assessed by measuring the intracellular core‐particle‐associated HBV <t>DNA</t> using real‐time PCR. (C, D) Dose‐response curves were drawn with the obtained data. The amount of core‐particle‐associated HBV DNA without TDF treatment was defined as the untreated control, and percentages of the amount of core‐particle‐associated HBV DNA at the indicated concentrations of TDF were calculated. The results are shown as the means ± SD.
    Full Length Hbv Dna Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length hbv dna fragment/product/Roche
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    full length hbv dna fragment - by Bioz Stars, 2020-09
    91/100 stars
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    Image Search Results


    ( a ) Schematic representation of the intramolecular ligation technique to obtain the preCore and YMDD Pol motif close to each other. ( 1 ) The circle in a continuous line represents the complete HBV genome (3200 bp). The semicircles in discontinuous lines represent the first (first PCR, 2700 bp) and the nested (2194 bp for genotype A2 sequences and 2155 bp for genotype D sequences) PCR products. ( 2 ) The product obtained after intramolecular ligation and primers used to sequence the circular molecule ( Table 2 ) are indicated in the figure. ( b ) Agarose gel obtained after intramolecular digestion. Two markers (M-I and M-II) were loaded in the gel; band lengths are shown at the left side of the image. Two samples (S1 and S2) were loaded and four bands were detected; band lengths are indicated at the right side of the image. A band with an apparent size of 1700 bp was the circular molecule, with the preCore and YMDD Pol motif close to each other. Band B was the nested PCR product without ligation, and band D were dimers of nested PCR products without ligation. Band C corresponded to the ligated dimers. B , C and D bands were not used, because the main interest was to analyze regions from the same genome. ( c ) Sequence of the circular construct including the preCore region, YMDD polymerase motif (in box), and HindIII sequence. PreCore (1, 2, 14, 15, 28 and 29) and Core ( 1 ) codons analyzed in the text are also enclosed in boxes.

    Journal: Nucleic Acids Research

    Article Title: Ultra-deep pyrosequencing analysis of the hepatitis B virus preCore region and main catalytic motif of the viral polymerase in the same viral genome

    doi: 10.1093/nar/gkr451

    Figure Lengend Snippet: ( a ) Schematic representation of the intramolecular ligation technique to obtain the preCore and YMDD Pol motif close to each other. ( 1 ) The circle in a continuous line represents the complete HBV genome (3200 bp). The semicircles in discontinuous lines represent the first (first PCR, 2700 bp) and the nested (2194 bp for genotype A2 sequences and 2155 bp for genotype D sequences) PCR products. ( 2 ) The product obtained after intramolecular ligation and primers used to sequence the circular molecule ( Table 2 ) are indicated in the figure. ( b ) Agarose gel obtained after intramolecular digestion. Two markers (M-I and M-II) were loaded in the gel; band lengths are shown at the left side of the image. Two samples (S1 and S2) were loaded and four bands were detected; band lengths are indicated at the right side of the image. A band with an apparent size of 1700 bp was the circular molecule, with the preCore and YMDD Pol motif close to each other. Band B was the nested PCR product without ligation, and band D were dimers of nested PCR products without ligation. Band C corresponded to the ligated dimers. B , C and D bands were not used, because the main interest was to analyze regions from the same genome. ( c ) Sequence of the circular construct including the preCore region, YMDD polymerase motif (in box), and HindIII sequence. PreCore (1, 2, 14, 15, 28 and 29) and Core ( 1 ) codons analyzed in the text are also enclosed in boxes.

    Article Snippet: Optimal results were obtained with 0.5 ng/µ1 of the HBV DNA-PCR fragment, and 1 U of T4 DNA ligase (Roche Diagnostics GmbH).

    Techniques: Ligation, Polymerase Chain Reaction, Sequencing, Agarose Gel Electrophoresis, Nested PCR, Construct

    Susceptibilities to TDF of ETV‐resistant clones identified in this study. HBV molecular clones derived from patients A and B were transfected into HepG2 cells, and TDF was administered to the transfected cells at the indicated concentrations. (A, B) HBV replication and its reduction by TDF administration were assessed by measuring the intracellular core‐particle‐associated HBV DNA using real‐time PCR. (C, D) Dose‐response curves were drawn with the obtained data. The amount of core‐particle‐associated HBV DNA without TDF treatment was defined as the untreated control, and percentages of the amount of core‐particle‐associated HBV DNA at the indicated concentrations of TDF were calculated. The results are shown as the means ± SD.

    Journal: Hepatology Communications

    Article Title: Resistance mutations of hepatitis B virus in entecavir‐refractory patients

    doi: 10.1002/hep4.1022

    Figure Lengend Snippet: Susceptibilities to TDF of ETV‐resistant clones identified in this study. HBV molecular clones derived from patients A and B were transfected into HepG2 cells, and TDF was administered to the transfected cells at the indicated concentrations. (A, B) HBV replication and its reduction by TDF administration were assessed by measuring the intracellular core‐particle‐associated HBV DNA using real‐time PCR. (C, D) Dose‐response curves were drawn with the obtained data. The amount of core‐particle‐associated HBV DNA without TDF treatment was defined as the untreated control, and percentages of the amount of core‐particle‐associated HBV DNA at the indicated concentrations of TDF were calculated. The results are shown as the means ± SD.

    Article Snippet: The transferred membrane was immobilized by an ultraviolet crosslinker and hybridized by an alkaline‐ phosphatase‐labeled probe of a full‐length HBV DNA fragment (accession number AB246344, https://www.ncbi.nlm.nih.gov/nuccore/AB246344 ) generated with the Gene Images AlkPhos Direct labeling system (DIG‐High Prime DNA Labeling and Detection Starter Kit II; Roche Diagnostics).

    Techniques: Clone Assay, Derivative Assay, Transfection, Real-time Polymerase Chain Reaction

    Dose‐response curve analysis of susceptibilities of patient‐derived and identified mutation‐introduced HBV clones (A: Case A with VBT, B: Case B with VBT, C: Case C with partial virological response, D: Case D with flare‐up). The dose‐response curves were drawn with data obtained by real‐time PCR described in Fig. 3 and Supporting Fig. S2 . The amount of core‐particle‐associated HBV DNA without ETV treatment was defined as the untreated control, and percentages of the amount of core‐particle‐associated HBV DNA at the indicated concentration of ETV were calculated. The results are shown as the means ± SD.

    Journal: Hepatology Communications

    Article Title: Resistance mutations of hepatitis B virus in entecavir‐refractory patients

    doi: 10.1002/hep4.1022

    Figure Lengend Snippet: Dose‐response curve analysis of susceptibilities of patient‐derived and identified mutation‐introduced HBV clones (A: Case A with VBT, B: Case B with VBT, C: Case C with partial virological response, D: Case D with flare‐up). The dose‐response curves were drawn with data obtained by real‐time PCR described in Fig. 3 and Supporting Fig. S2 . The amount of core‐particle‐associated HBV DNA without ETV treatment was defined as the untreated control, and percentages of the amount of core‐particle‐associated HBV DNA at the indicated concentration of ETV were calculated. The results are shown as the means ± SD.

    Article Snippet: The transferred membrane was immobilized by an ultraviolet crosslinker and hybridized by an alkaline‐ phosphatase‐labeled probe of a full‐length HBV DNA fragment (accession number AB246344, https://www.ncbi.nlm.nih.gov/nuccore/AB246344 ) generated with the Gene Images AlkPhos Direct labeling system (DIG‐High Prime DNA Labeling and Detection Starter Kit II; Roche Diagnostics).

    Techniques: Derivative Assay, Mutagenesis, Clone Assay, Real-time Polymerase Chain Reaction, Concentration Assay

    Replication of patient‐derived HBV molecular clones and susceptibility to ETV (A: Case A with VBT, B: Case B with VBT, C: Case C with partial virological response, D: Case D with flare‐up). HBV molecular clones were transfected into HepG2 cells, and ETV was administered to the transfected cells at the indicated concentrations. HBV replication and its reduction by ETV administration were assessed by measuring the intracellular core‐particle‐associated HBV DNA using Southern blotting (upper panel) and real‐time PCR (lower panel). The results are shown as the means ± SD. Abbreviations: DSL, double‐stranded liner HBV DNA; RC, relaxed circular HBV DNA; SS, single‐stranded HBV DNA.

    Journal: Hepatology Communications

    Article Title: Resistance mutations of hepatitis B virus in entecavir‐refractory patients

    doi: 10.1002/hep4.1022

    Figure Lengend Snippet: Replication of patient‐derived HBV molecular clones and susceptibility to ETV (A: Case A with VBT, B: Case B with VBT, C: Case C with partial virological response, D: Case D with flare‐up). HBV molecular clones were transfected into HepG2 cells, and ETV was administered to the transfected cells at the indicated concentrations. HBV replication and its reduction by ETV administration were assessed by measuring the intracellular core‐particle‐associated HBV DNA using Southern blotting (upper panel) and real‐time PCR (lower panel). The results are shown as the means ± SD. Abbreviations: DSL, double‐stranded liner HBV DNA; RC, relaxed circular HBV DNA; SS, single‐stranded HBV DNA.

    Article Snippet: The transferred membrane was immobilized by an ultraviolet crosslinker and hybridized by an alkaline‐ phosphatase‐labeled probe of a full‐length HBV DNA fragment (accession number AB246344, https://www.ncbi.nlm.nih.gov/nuccore/AB246344 ) generated with the Gene Images AlkPhos Direct labeling system (DIG‐High Prime DNA Labeling and Detection Starter Kit II; Roche Diagnostics).

    Techniques: Derivative Assay, Clone Assay, Transfection, Southern Blot, Real-time Polymerase Chain Reaction

    Clinical courses of 4 ETV‐refractory patients (A: Case A with VBT, B: Case B with VBT, C: Case C with partial virological response, D: Case D with flare‐up). Serum HBV DNA titers (solid squares) and ALT levels (open circles) are indicated. The lower detection limit of HBV DNA is indicated by the dotted line. The serum samples were collected at the time points indicated by open arrows with numbers, and the sequences of infected HBV were analyzed. Abbreviation: ALT, alanine aminotransferase.

    Journal: Hepatology Communications

    Article Title: Resistance mutations of hepatitis B virus in entecavir‐refractory patients

    doi: 10.1002/hep4.1022

    Figure Lengend Snippet: Clinical courses of 4 ETV‐refractory patients (A: Case A with VBT, B: Case B with VBT, C: Case C with partial virological response, D: Case D with flare‐up). Serum HBV DNA titers (solid squares) and ALT levels (open circles) are indicated. The lower detection limit of HBV DNA is indicated by the dotted line. The serum samples were collected at the time points indicated by open arrows with numbers, and the sequences of infected HBV were analyzed. Abbreviation: ALT, alanine aminotransferase.

    Article Snippet: The transferred membrane was immobilized by an ultraviolet crosslinker and hybridized by an alkaline‐ phosphatase‐labeled probe of a full‐length HBV DNA fragment (accession number AB246344, https://www.ncbi.nlm.nih.gov/nuccore/AB246344 ) generated with the Gene Images AlkPhos Direct labeling system (DIG‐High Prime DNA Labeling and Detection Starter Kit II; Roche Diagnostics).

    Techniques: Infection