hanks balanced salt solution hbss  (Thermo Fisher)


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    Name:
    Hanks Balanced Salt Solution HBSS without Ca2
    Description:
    The Thermo Scientific Pierce Hanks Balanced Salt Solution HBSS without Ca2 Mg2 is ideal for gently washing primary cells after digestion of the connective tissue The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat Features of the Thermo Scientific Pierce Hanks Balanced Salt Solution HBSS • Optimized procedure and reagents optimized for viability yield purity and ease of use • Time saving isolation protocol requires less than 2 hours compared to up to 21 hours by other methods • Yield provides a 1 5 to 4 fold increase in yield compared to do it yourself methods and other commercial kits • Viability provides higher viability than do it yourself methods and other commercial kits • Functional cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity Includes • Complete kit includes Cardiomyocyte Isolation Enzyme 1 Cardiomyocyte Isolation Enzyme 2 Hanks Balanced Salt Solution HBSS DMEM for Primary Cell Isolation and Cardiomyocyte Growth Supplement Applications • Cardiomyocyte cell differentiation • Immunohistochemistry IHC • Functional and biochemical assays • Biologically relevant system for preclinical drug discovery and predictive disease modeling The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing The kit contains two tissue specific dissociation enzymes media formulated for primary cell culture and a cardiomyocyte cell culture growth supplement that promotes the isolation enrichment and growth of functional cardiomyocytes The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes and cultures have been maintained for up to 7 days using the kit components Current methods for dissociation of cardiac cells from heart tissue require repeated 5 to 8 incubations or lengthy 16 hours enzyme digestion resulting in reduced yield and viability of isolated cells The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours Because it uses optimized tissue specific dissociation enzymes this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function beating activity see video thereby providing a model system for studies of contraction ischaemia hypoxia and the toxicity of various compounds More Product Data • One hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts Related Products Pierce Primary Cardiomyocyte Isolation Kit Cardiomyocyte Isolation Enzyme 1 with papain for Pierce Primary Cell Isolation Kits Cardiomyocyte Isolation Enzyme 2 with thermolysin 20X for Pierce Primary Cell Isolation Kits DMEM for Pierce Primary Cell Isolation Kits
    Catalog Number:
    88284
    Price:
    None
    Applications:
    Cell Culture|Primary Cell Culture
    Category:
    Cell Culture Transfection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher hanks balanced salt solution hbss
    DLS analysis of <t>PF4/UFH</t> and PF4/polyP 130 complexes . (A) PF4/UFH complexes. PF4 (50 µg/mL) was incubated with a range of concentrations of UFH (0.1 U/mL, 1 U/mL, and 10 U/m) in <t>HBSS</t> for 30 minutes at RT to create theoretically net positively charged, neutral, and negatively charged complexes. The resultant complexes were analyzed for size and distribution by DLS. Complexes formed with 10 U/m heparin were too unstable for analysis. The data shown here and all subsequent figures are representative of 2 to 3 independent experiments. (B) PF4/polyP 130 complexes. The same experiment was performed with PF4 incubated with 2 µM, 20 µM, and 200 µM polyP 130 , respectively. (C) Intrinsic stability of PF4/UFH and PF4/polyP 130 complexes. PF4 (5 µg/mL) was incubated with optimal antigenic concentrations of UFH (0.1 U/mL) or polyP 130 (3 µM) was incubated for 30 minutes at RT and the complexes were analyzed using DLS analysis 30 minutes or 96 hours later. Essentially identical protection was seen at 120 hours. (D) Binding of KKO to PF4/polyP 130 complexes. Complexes composed of PF4 (5 µg/mL) and polyP 130 (3 µM) were incubated for 1 hour with buffer alone or with KKO (1.6-16 µg/mL at RT before DLS analysis). (E) Effect of KKO on the intrinsic stability of PF4/polyP 130 complexes. Complexes were formed between PF4 (5 µg/mL) and polyP 130 (3 µM) as in panel D; DLS analysis was performed 1 and 120 hours later. (F) Effect of KKO on the susceptibility of PF4/polyP 130 complexes to phosphatases. Complexes were formed between PF4 (5 µg/mL) and polyP 130 (3 µM) as described in panel D. The complexes were then incubated with KKO (5 µg) or buffer for 30 minutes at RT; CIP (200 units) or buffer was added for 1 hour and the DLS analysis was repeated. d, diameter.
    The Thermo Scientific Pierce Hanks Balanced Salt Solution HBSS without Ca2 Mg2 is ideal for gently washing primary cells after digestion of the connective tissue The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat Features of the Thermo Scientific Pierce Hanks Balanced Salt Solution HBSS • Optimized procedure and reagents optimized for viability yield purity and ease of use • Time saving isolation protocol requires less than 2 hours compared to up to 21 hours by other methods • Yield provides a 1 5 to 4 fold increase in yield compared to do it yourself methods and other commercial kits • Viability provides higher viability than do it yourself methods and other commercial kits • Functional cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity Includes • Complete kit includes Cardiomyocyte Isolation Enzyme 1 Cardiomyocyte Isolation Enzyme 2 Hanks Balanced Salt Solution HBSS DMEM for Primary Cell Isolation and Cardiomyocyte Growth Supplement Applications • Cardiomyocyte cell differentiation • Immunohistochemistry IHC • Functional and biochemical assays • Biologically relevant system for preclinical drug discovery and predictive disease modeling The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing The kit contains two tissue specific dissociation enzymes media formulated for primary cell culture and a cardiomyocyte cell culture growth supplement that promotes the isolation enrichment and growth of functional cardiomyocytes The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes and cultures have been maintained for up to 7 days using the kit components Current methods for dissociation of cardiac cells from heart tissue require repeated 5 to 8 incubations or lengthy 16 hours enzyme digestion resulting in reduced yield and viability of isolated cells The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours Because it uses optimized tissue specific dissociation enzymes this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function beating activity see video thereby providing a model system for studies of contraction ischaemia hypoxia and the toxicity of various compounds More Product Data • One hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts Related Products Pierce Primary Cardiomyocyte Isolation Kit Cardiomyocyte Isolation Enzyme 1 with papain for Pierce Primary Cell Isolation Kits Cardiomyocyte Isolation Enzyme 2 with thermolysin 20X for Pierce Primary Cell Isolation Kits DMEM for Pierce Primary Cell Isolation Kits
    https://www.bioz.com/result/hanks balanced salt solution hbss/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    hanks balanced salt solution hbss - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Polyphosphate/platelet factor 4 complexes can mediate heparin-independent platelet activation in heparin-induced thrombocytopenia"

    Article Title: Polyphosphate/platelet factor 4 complexes can mediate heparin-independent platelet activation in heparin-induced thrombocytopenia

    Journal: Blood Advances

    doi: 10.1182/bloodadvances.2016000877

    DLS analysis of PF4/UFH and PF4/polyP 130 complexes . (A) PF4/UFH complexes. PF4 (50 µg/mL) was incubated with a range of concentrations of UFH (0.1 U/mL, 1 U/mL, and 10 U/m) in HBSS for 30 minutes at RT to create theoretically net positively charged, neutral, and negatively charged complexes. The resultant complexes were analyzed for size and distribution by DLS. Complexes formed with 10 U/m heparin were too unstable for analysis. The data shown here and all subsequent figures are representative of 2 to 3 independent experiments. (B) PF4/polyP 130 complexes. The same experiment was performed with PF4 incubated with 2 µM, 20 µM, and 200 µM polyP 130 , respectively. (C) Intrinsic stability of PF4/UFH and PF4/polyP 130 complexes. PF4 (5 µg/mL) was incubated with optimal antigenic concentrations of UFH (0.1 U/mL) or polyP 130 (3 µM) was incubated for 30 minutes at RT and the complexes were analyzed using DLS analysis 30 minutes or 96 hours later. Essentially identical protection was seen at 120 hours. (D) Binding of KKO to PF4/polyP 130 complexes. Complexes composed of PF4 (5 µg/mL) and polyP 130 (3 µM) were incubated for 1 hour with buffer alone or with KKO (1.6-16 µg/mL at RT before DLS analysis). (E) Effect of KKO on the intrinsic stability of PF4/polyP 130 complexes. Complexes were formed between PF4 (5 µg/mL) and polyP 130 (3 µM) as in panel D; DLS analysis was performed 1 and 120 hours later. (F) Effect of KKO on the susceptibility of PF4/polyP 130 complexes to phosphatases. Complexes were formed between PF4 (5 µg/mL) and polyP 130 (3 µM) as described in panel D. The complexes were then incubated with KKO (5 µg) or buffer for 30 minutes at RT; CIP (200 units) or buffer was added for 1 hour and the DLS analysis was repeated. d, diameter.
    Figure Legend Snippet: DLS analysis of PF4/UFH and PF4/polyP 130 complexes . (A) PF4/UFH complexes. PF4 (50 µg/mL) was incubated with a range of concentrations of UFH (0.1 U/mL, 1 U/mL, and 10 U/m) in HBSS for 30 minutes at RT to create theoretically net positively charged, neutral, and negatively charged complexes. The resultant complexes were analyzed for size and distribution by DLS. Complexes formed with 10 U/m heparin were too unstable for analysis. The data shown here and all subsequent figures are representative of 2 to 3 independent experiments. (B) PF4/polyP 130 complexes. The same experiment was performed with PF4 incubated with 2 µM, 20 µM, and 200 µM polyP 130 , respectively. (C) Intrinsic stability of PF4/UFH and PF4/polyP 130 complexes. PF4 (5 µg/mL) was incubated with optimal antigenic concentrations of UFH (0.1 U/mL) or polyP 130 (3 µM) was incubated for 30 minutes at RT and the complexes were analyzed using DLS analysis 30 minutes or 96 hours later. Essentially identical protection was seen at 120 hours. (D) Binding of KKO to PF4/polyP 130 complexes. Complexes composed of PF4 (5 µg/mL) and polyP 130 (3 µM) were incubated for 1 hour with buffer alone or with KKO (1.6-16 µg/mL at RT before DLS analysis). (E) Effect of KKO on the intrinsic stability of PF4/polyP 130 complexes. Complexes were formed between PF4 (5 µg/mL) and polyP 130 (3 µM) as in panel D; DLS analysis was performed 1 and 120 hours later. (F) Effect of KKO on the susceptibility of PF4/polyP 130 complexes to phosphatases. Complexes were formed between PF4 (5 µg/mL) and polyP 130 (3 µM) as described in panel D. The complexes were then incubated with KKO (5 µg) or buffer for 30 minutes at RT; CIP (200 units) or buffer was added for 1 hour and the DLS analysis was repeated. d, diameter.

    Techniques Used: Incubation, Binding Assay

    2) Product Images from "Nod-Like Receptor X-1 Is Required for Rhinovirus-Induced Barrier Dysfunction in Airway Epithelial Cells"

    Article Title: Nod-Like Receptor X-1 Is Required for Rhinovirus-Induced Barrier Dysfunction in Airway Epithelial Cells

    Journal: Journal of Virology

    doi: 10.1128/JVI.03039-13

    RV stimulates NLRX-1-dependent mitochondrial ROS in polarized airway epithelial cells. The polarized monolayers of 16HBE14o− cells were infected with RV or sham control and incubated for 90 min at 33°C. The infection medium was replaced with fresh medium, and incubation continued for an additional 16 h. The cells were washed once with HBSS and incubated with 5 μM MitoSox Red for 15 min at 37°C. The cells were washed with warm HBSS. (A) Cells were incubated with HBSS containing Hoechst dye (nuclear stain) for 10 min, which was then replaced with warm HBSS, and the cells were imaged immediately. The images are representative of 3 independent experiments (red, MitoSox Red fluorescence indicative of mitochondrial ROS; blue, nuclei). (B and C) Cells were detached from the plate, suspended in warm HBSS, and analyzed by flow cytometry. MFI, mean fluorescence intensity. (D) NT or NLRX-1 siRNA-transfected cells were infected with sham control or RV, and mitochondrial ROS was quantified by flow cytometry 16 h postinfection. (B) Representative histogram of 3 independent experiments. (C and D) Data represent means and SD calculated from 3 or 4 independent experiments (*, P ≤ 0.05; ANOVA).
    Figure Legend Snippet: RV stimulates NLRX-1-dependent mitochondrial ROS in polarized airway epithelial cells. The polarized monolayers of 16HBE14o− cells were infected with RV or sham control and incubated for 90 min at 33°C. The infection medium was replaced with fresh medium, and incubation continued for an additional 16 h. The cells were washed once with HBSS and incubated with 5 μM MitoSox Red for 15 min at 37°C. The cells were washed with warm HBSS. (A) Cells were incubated with HBSS containing Hoechst dye (nuclear stain) for 10 min, which was then replaced with warm HBSS, and the cells were imaged immediately. The images are representative of 3 independent experiments (red, MitoSox Red fluorescence indicative of mitochondrial ROS; blue, nuclei). (B and C) Cells were detached from the plate, suspended in warm HBSS, and analyzed by flow cytometry. MFI, mean fluorescence intensity. (D) NT or NLRX-1 siRNA-transfected cells were infected with sham control or RV, and mitochondrial ROS was quantified by flow cytometry 16 h postinfection. (B) Representative histogram of 3 independent experiments. (C and D) Data represent means and SD calculated from 3 or 4 independent experiments (*, P ≤ 0.05; ANOVA).

    Techniques Used: Infection, Incubation, Staining, Fluorescence, Flow Cytometry, Cytometry, Transfection

    3) Product Images from "Virulence Attenuation of Candida albicans Genetic Variants Isolated from a Patient with a Recurrent Bloodstream Infection"

    Article Title: Virulence Attenuation of Candida albicans Genetic Variants Isolated from a Patient with a Recurrent Bloodstream Infection

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0010155

    Kidney fungal burden. Groups of four mice infected i.v. with 10 6 C. albicans cells were killed at 1, 3 and 7 days after challenge. Organs were homogenized in HBSS and the suspension diluted and cultured on Sabouraud dextrose agar. Results are presented as log of colony-forming units (CFUs). Statistically significant differences between results at each hour of infection as evaluated by Student's t test are labeled with single asterisk ( P
    Figure Legend Snippet: Kidney fungal burden. Groups of four mice infected i.v. with 10 6 C. albicans cells were killed at 1, 3 and 7 days after challenge. Organs were homogenized in HBSS and the suspension diluted and cultured on Sabouraud dextrose agar. Results are presented as log of colony-forming units (CFUs). Statistically significant differences between results at each hour of infection as evaluated by Student's t test are labeled with single asterisk ( P

    Techniques Used: Mouse Assay, Infection, Cell Culture, Labeling

    4) Product Images from "The Effect of the Nonionic Block Copolymer Pluronic P85 on Gene Expression in Mouse Muscle and Antigen Presenting Cells"

    Article Title: The Effect of the Nonionic Block Copolymer Pluronic P85 on Gene Expression in Mouse Muscle and Antigen Presenting Cells

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2008.10.064

    Dose dependent effect of various poloxamers on luciferase gene expression in skeletal ( tibialis anterior ) muscle of BALB/c mice after single i.m. injection of 10 μg plasmid DNA in 50 μl HBSS solution with or without block copolymers (5 mice/group). Muscles tissue was harvested on day 4 after i.m. injection and luciferase activity was measured using a standard procedure described in the Methods section. Data are mean ± SEM (n = 5).The P values were obtained by the means of the Student's t test following logarithmic transformation of the data. Each P value corresponds to the comparison of naked DNA versus DNA formulated with P85: * p
    Figure Legend Snippet: Dose dependent effect of various poloxamers on luciferase gene expression in skeletal ( tibialis anterior ) muscle of BALB/c mice after single i.m. injection of 10 μg plasmid DNA in 50 μl HBSS solution with or without block copolymers (5 mice/group). Muscles tissue was harvested on day 4 after i.m. injection and luciferase activity was measured using a standard procedure described in the Methods section. Data are mean ± SEM (n = 5).The P values were obtained by the means of the Student's t test following logarithmic transformation of the data. Each P value corresponds to the comparison of naked DNA versus DNA formulated with P85: * p

    Techniques Used: Luciferase, Expressing, Mouse Assay, Injection, Plasmid Preparation, Blocking Assay, Activity Assay, Transformation Assay

    CD49+, CD3e+, CD8a+, CD4+, CD14+, and CD11c+ cell frequency in spleen (A) and lymph nodes (B) of Balb/c mice injected with HBSS, DNA, P85 and DNA/P85. The cells frequency was determined by flow cytometry on day 4 after single i.m. injection. Data are mean ± SEM (n = 5). Statistical comparisons between the groups are made using ANOVA: * p
    Figure Legend Snippet: CD49+, CD3e+, CD8a+, CD4+, CD14+, and CD11c+ cell frequency in spleen (A) and lymph nodes (B) of Balb/c mice injected with HBSS, DNA, P85 and DNA/P85. The cells frequency was determined by flow cytometry on day 4 after single i.m. injection. Data are mean ± SEM (n = 5). Statistical comparisons between the groups are made using ANOVA: * p

    Techniques Used: Mouse Assay, Injection, Flow Cytometry, Cytometry

    Effect of 0.3% P85 on luciferase gene expression in vivo after a single i.m. injection of 10 μg plasmid DNA in 50 μl HBSS. (A) A representative in vivo imaging in a mouse. (B) Quantitative data of in vivo imaging study. Data are mean ± SEM (n = 5). P values were obtained by the means of the Student's t test following logarithmic transformation of the data. Each P value corresponds to the comparison of naked DNA versus DNA formulated with P85: * p
    Figure Legend Snippet: Effect of 0.3% P85 on luciferase gene expression in vivo after a single i.m. injection of 10 μg plasmid DNA in 50 μl HBSS. (A) A representative in vivo imaging in a mouse. (B) Quantitative data of in vivo imaging study. Data are mean ± SEM (n = 5). P values were obtained by the means of the Student's t test following logarithmic transformation of the data. Each P value corresponds to the comparison of naked DNA versus DNA formulated with P85: * p

    Techniques Used: Luciferase, Expressing, In Vivo, Injection, Plasmid Preparation, In Vivo Imaging, Transformation Assay

    Effects of 0.3 % P85 on (A) GFP or (B) luciferase gene expression, and (C) DNA or (D) RNA levels in various organs. Tissue samples were collected on day 4 after single i.m. injections of 10 μg DNA in 50 μl HBSS solution with/without copolymer. (B) Data are mean ± SEM (n = 5). P values were obtained by the means of the Student's t test. Each P value corresponds to the comparison of naked DNA versus DNA formulated with P85: * p
    Figure Legend Snippet: Effects of 0.3 % P85 on (A) GFP or (B) luciferase gene expression, and (C) DNA or (D) RNA levels in various organs. Tissue samples were collected on day 4 after single i.m. injections of 10 μg DNA in 50 μl HBSS solution with/without copolymer. (B) Data are mean ± SEM (n = 5). P values were obtained by the means of the Student's t test. Each P value corresponds to the comparison of naked DNA versus DNA formulated with P85: * p

    Techniques Used: Luciferase, Expressing

    5) Product Images from "Pseudomonas aeruginosa Suppresses Interferon Response to Rhinovirus Infection in Cystic Fibrosis but Not in Normal Bronchial Epithelial Cells ▿ Suppresses Interferon Response to Rhinovirus Infection in Cystic Fibrosis but Not in Normal Bronchial Epithelial Cells ▿ §"

    Article Title: Pseudomonas aeruginosa Suppresses Interferon Response to Rhinovirus Infection in Cystic Fibrosis but Not in Normal Bronchial Epithelial Cells ▿ Suppresses Interferon Response to Rhinovirus Infection in Cystic Fibrosis but Not in Normal Bronchial Epithelial Cells ▿ §

    Journal: Infection and Immunity

    doi: 10.1128/IAI.05120-11

    Antioxidant treatment decreases ROS in IB3 cells and reverses the suppressive effect of MPA on RV-induced IFN responses in IB3 cells. IB3 or BEAS-2B cells grown as monolayers were incubated with medium or MPA for 6 h in the presence or absence of DPI or NAC. Cells were then incubated with carboxy-H 2 DCFDA for 30 min in HBSS, washed, and analyzed by flow cytometry. Representative histogram (A) and mean fluorescence intensity (B and C) from three independent experiments showing increased levels of ROS in IB3 cells, both basal and postinfection with MPA. ROS generation in uninfected and MPA-infected IB3 cells in the presence or absence of DPI or NAC (C). IB3 cells treated with medium, DMSO, DPI, or NAC for 6 h were infected with MPA and RV as described above in the absence of antioxidants, and IFN expression (D, E, and F) and viral load (G) were determined as described above. Data represent averages ± SD or ranges with medians calculated from two or three independent experiments performed in duplicate. *, different from UVRV-infected group ( P ≤ 0.05); †, significantly different from cells infected with RV alone ( P ≤ 0.05); ‡, different from cells not pretreated with DPI or NAC ( P ≤ 0.05) (ANOVA). Representative blot showing increased phosphorylation of Akt and IRF3 in IB3 cells coinfected with MPA/RV in the presence of DPI (H).
    Figure Legend Snippet: Antioxidant treatment decreases ROS in IB3 cells and reverses the suppressive effect of MPA on RV-induced IFN responses in IB3 cells. IB3 or BEAS-2B cells grown as monolayers were incubated with medium or MPA for 6 h in the presence or absence of DPI or NAC. Cells were then incubated with carboxy-H 2 DCFDA for 30 min in HBSS, washed, and analyzed by flow cytometry. Representative histogram (A) and mean fluorescence intensity (B and C) from three independent experiments showing increased levels of ROS in IB3 cells, both basal and postinfection with MPA. ROS generation in uninfected and MPA-infected IB3 cells in the presence or absence of DPI or NAC (C). IB3 cells treated with medium, DMSO, DPI, or NAC for 6 h were infected with MPA and RV as described above in the absence of antioxidants, and IFN expression (D, E, and F) and viral load (G) were determined as described above. Data represent averages ± SD or ranges with medians calculated from two or three independent experiments performed in duplicate. *, different from UVRV-infected group ( P ≤ 0.05); †, significantly different from cells infected with RV alone ( P ≤ 0.05); ‡, different from cells not pretreated with DPI or NAC ( P ≤ 0.05) (ANOVA). Representative blot showing increased phosphorylation of Akt and IRF3 in IB3 cells coinfected with MPA/RV in the presence of DPI (H).

    Techniques Used: Incubation, Flow Cytometry, Cytometry, Fluorescence, Infection, Expressing

    Related Articles

    Blocking Assay:

    Article Title: The Effect of the Nonionic Block Copolymer Pluronic P85 on Gene Expression in Mouse Muscle and Antigen Presenting Cells
    Article Snippet: .. The plasmid DNA and block copolymer stock solutions were added to Gibco™ Hanks Balanced Salt Solution (HBSS) buffer (Invitrogen, Carlsbad, CA) and gently mixed to obtain required concentrations (in case of SP1017 the total block copolymer concentrations at various dilutions are presented). ..

    Incubation:

    Article Title: Pseudomonas aeruginosa Suppresses Interferon Response to Rhinovirus Infection in Cystic Fibrosis but Not in Normal Bronchial Epithelial Cells ▿ Suppresses Interferon Response to Rhinovirus Infection in Cystic Fibrosis but Not in Normal Bronchial Epithelial Cells ▿ §
    Article Snippet: .. After the relevant treatment, cells were incubated with 2 μM carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2 DCFDA; Invitrogen) in Hanks balanced salt solution (HBSS) for 30 min. .. Cells were then shifted to serum-free medium and incubated for 1 h. Cells were collected and suspended in PBS, fluorescent emission was quantified by flow cytometry, and the results were analyzed using CellQuest software.

    Article Title: Nod-Like Receptor X-1 Is Required for Rhinovirus-Induced Barrier Dysfunction in Airway Epithelial Cells
    Article Snippet: .. After relevant treatment, the cells were briefly washed with Hanks balanced salt solution (HBSS) and incubated with 5 μM MitoSox Red (Life Technologies, Grand Island, NY) for 15 min. .. The cells were washed and counterstained with Hoechst stain, and live cells were immediately imaged using confocal microscopy.

    other:

    Article Title: Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor
    Article Snippet: Hanks’ balanced salt solution (HBSS) with or without Ca2+ and fura-2, AM were acquired from Life Technologies (Carlsbad, CA).

    Cell Culture:

    Article Title: Virulence Attenuation of Candida albicans Genetic Variants Isolated from a Patient with a Recurrent Bloodstream Infection
    Article Snippet: .. Organs were homogenized in 2 ml of Hanks Balanced Salt Solution (HBSS) from Invitrogen, diluted, and cultured on Sabouraud agar at 37°C. ..

    Modification:

    Article Title: Human astrocytes/astrocyte conditioned medium and shear stress enhance the barrier properties of human brain microvascular endothelial cells
    Article Snippet: .. Medium 199, Modified Eagle's Medium (MEM), penicillin and streptomycin, trypsin, Hanks Balanced Salt Solution (HBSS) and SDSPAGE gels were purchased from Invitrogen Corporation (Carlsbad, CA). .. Heat inactivated fetal bovine serum (FBS) was purchased from Omega scientific Inc. (Tarzena, CA), and Nu Serum was from BD Biosciences (Bedford, MA).

    Lysis:

    Article Title: Erythropoietin increases macrophage-mediated T cell suppression
    Article Snippet: .. Red blood cells were removed from SP cell preparations by hypertonic lysis followed by washing with Hanks Balanced Salt Solution (HBSS) (Life Technologies, Grand Island, NY). .. Peritoneal cavity (PerC) cells were obtained by flushing the peritoneum with 10 ml of warm (37°C) HBSS supplemented with 2–3% fetal bovine serum (FBS) (Hyclone, Logan, UT).

    Plasmid Preparation:

    Article Title: The Effect of the Nonionic Block Copolymer Pluronic P85 on Gene Expression in Mouse Muscle and Antigen Presenting Cells
    Article Snippet: .. The plasmid DNA and block copolymer stock solutions were added to Gibco™ Hanks Balanced Salt Solution (HBSS) buffer (Invitrogen, Carlsbad, CA) and gently mixed to obtain required concentrations (in case of SP1017 the total block copolymer concentrations at various dilutions are presented). ..

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  • 99
    Thermo Fisher hbss
    The scheme of the enucleation protocol. a Cells growing on plastic slides were inserted in 1.5 mL Eppendorf tubes filled with the cell medium and pre-treated with CytD for 15 min. After the centrifugation, the remaining cells and cytoplasts were reseeded from the plastic slides to fibronectin-coated Petri dishes, and AFM experiments were performed 3 to 5 h after. The pellet containing <t>nucleoplasts</t> was resuspended in <t>HBSS</t> and placed on poly-L-lysine-coated dishes for further experiments. b A sample of REF52 enucleated cells contained both cells without the nucleus (enucleated cells, cytoplasts) and cells with the retained nucleus. The cell-permeable DNA dye (cyan) was used to distinguish cytoplasts from normal cells. c Nucleoplasts. DNA staining was used to distinguish nucleoplasts from the cell debris. Scale bars are 50 µm
    Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss/product/Thermo Fisher
    Average 99 stars, based on 361 article reviews
    Price from $9.99 to $1999.99
    hbss - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher hanks balanced salt solution hbss
    The scheme of the enucleation protocol. a Cells growing on plastic slides were inserted in 1.5 mL Eppendorf tubes filled with the cell medium and pre-treated with CytD for 15 min. After the centrifugation, the remaining cells and cytoplasts were reseeded from the plastic slides to fibronectin-coated Petri dishes, and AFM experiments were performed 3 to 5 h after. The pellet containing <t>nucleoplasts</t> was resuspended in <t>HBSS</t> and placed on poly-L-lysine-coated dishes for further experiments. b A sample of REF52 enucleated cells contained both cells without the nucleus (enucleated cells, cytoplasts) and cells with the retained nucleus. The cell-permeable DNA dye (cyan) was used to distinguish cytoplasts from normal cells. c Nucleoplasts. DNA staining was used to distinguish nucleoplasts from the cell debris. Scale bars are 50 µm
    Hanks Balanced Salt Solution Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hanks balanced salt solution hbss/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    hanks balanced salt solution hbss - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    The scheme of the enucleation protocol. a Cells growing on plastic slides were inserted in 1.5 mL Eppendorf tubes filled with the cell medium and pre-treated with CytD for 15 min. After the centrifugation, the remaining cells and cytoplasts were reseeded from the plastic slides to fibronectin-coated Petri dishes, and AFM experiments were performed 3 to 5 h after. The pellet containing nucleoplasts was resuspended in HBSS and placed on poly-L-lysine-coated dishes for further experiments. b A sample of REF52 enucleated cells contained both cells without the nucleus (enucleated cells, cytoplasts) and cells with the retained nucleus. The cell-permeable DNA dye (cyan) was used to distinguish cytoplasts from normal cells. c Nucleoplasts. DNA staining was used to distinguish nucleoplasts from the cell debris. Scale bars are 50 µm

    Journal: Journal of Nanobiotechnology

    Article Title: Nanomechanical properties of enucleated cells: contribution of the nucleus to the passive cell mechanics

    doi: 10.1186/s12951-020-00696-1

    Figure Lengend Snippet: The scheme of the enucleation protocol. a Cells growing on plastic slides were inserted in 1.5 mL Eppendorf tubes filled with the cell medium and pre-treated with CytD for 15 min. After the centrifugation, the remaining cells and cytoplasts were reseeded from the plastic slides to fibronectin-coated Petri dishes, and AFM experiments were performed 3 to 5 h after. The pellet containing nucleoplasts was resuspended in HBSS and placed on poly-L-lysine-coated dishes for further experiments. b A sample of REF52 enucleated cells contained both cells without the nucleus (enucleated cells, cytoplasts) and cells with the retained nucleus. The cell-permeable DNA dye (cyan) was used to distinguish cytoplasts from normal cells. c Nucleoplasts. DNA staining was used to distinguish nucleoplasts from the cell debris. Scale bars are 50 µm

    Article Snippet: The pellet containing nucleoplasts was resuspended in HBSS (Hanks balanced salt solution, Thermo Fischer, USA) and placed on poly-L-lysine-coated glass-bottom dishes.

    Techniques: Centrifugation, Staining