hank s balanced salt solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hank s balanced salt solution
    Confocal images of dendrimer in the brain when injected through the carotid artery. Neurons and glial cells labeled with propidium iodide (PI) stain (red— A , D , G ), <t>Hank’s</t> balanced salt solution (HBSS) control and merge ( B , C ), G4-90/10 infecting cells (arrow; E ) and merge (arrow; F ), G4-90/10 surrounding the blood vessel (arrow; H ) and merge (arrow; I ). All images were taken at 40× magnification using Olympus BX50 Upright Microscope (Olympus, Shinjuku, Tokyo, Japan). Scale bar: 100 µm.
    Hank S Balanced Salt Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1048 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "PAMAM Dendrimers Cross the Blood–Brain Barrier When Administered through the Carotid Artery in C57BL/6J Mice"

    Article Title: PAMAM Dendrimers Cross the Blood–Brain Barrier When Administered through the Carotid Artery in C57BL/6J Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18030628

    Confocal images of dendrimer in the brain when injected through the carotid artery. Neurons and glial cells labeled with propidium iodide (PI) stain (red— A , D , G ), Hank’s balanced salt solution (HBSS) control and merge ( B , C ), G4-90/10 infecting cells (arrow; E ) and merge (arrow; F ), G4-90/10 surrounding the blood vessel (arrow; H ) and merge (arrow; I ). All images were taken at 40× magnification using Olympus BX50 Upright Microscope (Olympus, Shinjuku, Tokyo, Japan). Scale bar: 100 µm.
    Figure Legend Snippet: Confocal images of dendrimer in the brain when injected through the carotid artery. Neurons and glial cells labeled with propidium iodide (PI) stain (red— A , D , G ), Hank’s balanced salt solution (HBSS) control and merge ( B , C ), G4-90/10 infecting cells (arrow; E ) and merge (arrow; F ), G4-90/10 surrounding the blood vessel (arrow; H ) and merge (arrow; I ). All images were taken at 40× magnification using Olympus BX50 Upright Microscope (Olympus, Shinjuku, Tokyo, Japan). Scale bar: 100 µm.

    Techniques Used: Injection, Labeling, Staining, Microscopy

    2) Product Images from "Long-term phenotypic correction in factor IX knockout mice by using phiC31 integrase-mediated gene therapy"

    Article Title: Long-term phenotypic correction in factor IX knockout mice by using phiC31 integrase-mediated gene therapy

    Journal: Gene therapy

    doi: 10.1038/gt.2011.31

    Evidence for prolonged hFIX expression and integration into a genomic pseudo attP site in mouse hepatocytes. ( a ) Immunofluorescence staining for hFIX in representative FIX −/− mouse liver sections obtained 200 days after HTV injection. The top row shows 4,6-diamidino-2-phenylindole (DAPI)-stained nuclei, the middle row shows staining for hFIX and the bottom row is a merged image of the top and middle rows. Vertical panel 1, Hank’s balanced salt solution (HBSS); panel 2, pVB9 alone; panel 3, pVI+pVB9; and panel 4, pVmI+pVB9. ( b ) PCR was carried out on DNA extracted from the liver, using primers that detect the junction between the attB site of pVB9 and mpsL1, a preferred pseudo attP site in the mouse genome. Lane 1, DNA ladder; lane 2, no template DNA control; lane 3, liver DNA sample of an injected animal from another experiment, previously shown to have attB -plasmid DNA integrated at mpsL1; lane 4, liver that was injected with HBSS; lane 5, liver that received pVB9 alone; lane 6, liver that received pVI+pVB9; and lane 7, liver that received pVmI+pVB9.
    Figure Legend Snippet: Evidence for prolonged hFIX expression and integration into a genomic pseudo attP site in mouse hepatocytes. ( a ) Immunofluorescence staining for hFIX in representative FIX −/− mouse liver sections obtained 200 days after HTV injection. The top row shows 4,6-diamidino-2-phenylindole (DAPI)-stained nuclei, the middle row shows staining for hFIX and the bottom row is a merged image of the top and middle rows. Vertical panel 1, Hank’s balanced salt solution (HBSS); panel 2, pVB9 alone; panel 3, pVI+pVB9; and panel 4, pVmI+pVB9. ( b ) PCR was carried out on DNA extracted from the liver, using primers that detect the junction between the attB site of pVB9 and mpsL1, a preferred pseudo attP site in the mouse genome. Lane 1, DNA ladder; lane 2, no template DNA control; lane 3, liver DNA sample of an injected animal from another experiment, previously shown to have attB -plasmid DNA integrated at mpsL1; lane 4, liver that was injected with HBSS; lane 5, liver that received pVB9 alone; lane 6, liver that received pVI+pVB9; and lane 7, liver that received pVmI+pVB9.

    Techniques Used: Expressing, Immunofluorescence, Staining, Injection, Polymerase Chain Reaction, Plasmid Preparation

    3) Product Images from "Inhibition of Bacterial Adhesion on Nanotextured Stainless Steel 316L by Electrochemical Etching"

    Article Title: Inhibition of Bacterial Adhesion on Nanotextured Stainless Steel 316L by Electrochemical Etching

    Journal: ACS Biomaterials Science & Engineering

    doi: 10.1021/acsbiomaterials.7b00544

    Corrosion behavior of SS316L samples in Hank’s balanced salt solution and chemical composition of SS316L surfaces. Potentiodynamic polarization curves of (a) AR- and (b) NT-SS316L samples and (c) representative curves for each SS316L sample. XPS spectra of (d) Cr 2p scans, (e) Mo 3d scans, and (f) O 1s scans of AR- and NT-SS316L surfaces.
    Figure Legend Snippet: Corrosion behavior of SS316L samples in Hank’s balanced salt solution and chemical composition of SS316L surfaces. Potentiodynamic polarization curves of (a) AR- and (b) NT-SS316L samples and (c) representative curves for each SS316L sample. XPS spectra of (d) Cr 2p scans, (e) Mo 3d scans, and (f) O 1s scans of AR- and NT-SS316L surfaces.

    Techniques Used:

    4) Product Images from "Novel delivery system for T-oligo using a nanocomplex formed with an alpha helical peptide for melanoma therapy"

    Article Title: Novel delivery system for T-oligo using a nanocomplex formed with an alpha helical peptide for melanoma therapy

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S55133

    Uptake studies of T-oligo and TOP complex in melanoma cells using fluorescence-activated cell sorting analysis and fluorescence microscopy. ( A ) 1.5×10 5 MM-AN cells in Minimum Essential Medium were plated in 35 mm 2 cell culture dishes. After 24 hours, the cells were treated with 40 μM FITC-T-oligo or the TOP complex (40 μM FITC-T-oligo and 0.025 mg/mL PVBLG) in Minimum Essential Medium with 2% calf serum for 4 hours. Cells were then washed twice with Hank’s Balanced Salt Solution, trypsinized, pelleted, counted, and fixed with 4% paraformaldehyde and analyzed on a Becton Dickinson fluorescent-activated cell sorter. Data analysis was performed using Cell Quest software. ( B ) 10,000 MM-AN cells were plated in chamber slides, treated with T-oligo or the TOP complex as described above, and observed under a fluorescent microscope (Olympus IX71) at 200× magnification. Abbreviations: C-oligo, complementary oligonucleotide; TOP complex, T-oligo and PVBLG nanocomplex; FITC, fluorescein isothiocyanate; FL1-H, fluorescence 1 signal height.
    Figure Legend Snippet: Uptake studies of T-oligo and TOP complex in melanoma cells using fluorescence-activated cell sorting analysis and fluorescence microscopy. ( A ) 1.5×10 5 MM-AN cells in Minimum Essential Medium were plated in 35 mm 2 cell culture dishes. After 24 hours, the cells were treated with 40 μM FITC-T-oligo or the TOP complex (40 μM FITC-T-oligo and 0.025 mg/mL PVBLG) in Minimum Essential Medium with 2% calf serum for 4 hours. Cells were then washed twice with Hank’s Balanced Salt Solution, trypsinized, pelleted, counted, and fixed with 4% paraformaldehyde and analyzed on a Becton Dickinson fluorescent-activated cell sorter. Data analysis was performed using Cell Quest software. ( B ) 10,000 MM-AN cells were plated in chamber slides, treated with T-oligo or the TOP complex as described above, and observed under a fluorescent microscope (Olympus IX71) at 200× magnification. Abbreviations: C-oligo, complementary oligonucleotide; TOP complex, T-oligo and PVBLG nanocomplex; FITC, fluorescein isothiocyanate; FL1-H, fluorescence 1 signal height.

    Techniques Used: Fluorescence, FACS, Microscopy, Cell Culture, Software

    5) Product Images from "TRPM7 promotes the epithelial–mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling"

    Article Title: TRPM7 promotes the epithelial–mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-019-1061-y

    TRPM7 silencing reduces the intracellular calcium concentrations in ovarian cancer cells. ( a )The fluorescence curves of the SKOV3 and OVCAR3 cells. ‘a’, cells that did not receive any treatment, and were exposed to Ca2 + −free HANK’s solution after uploaded the Fluo-8 AM fluorescence kit. The fluorescence curve of those cells is used as baseline for [Ca2+]I; ‘b’, the cells were treated with MK886 for 24 h, and exposed to HBSS (with CaCl2) after using the Fluo-3 AM fluorescence kit; ‘c’, the cells did not receive any treatment, but were exposed to HBSS (with CaCl2) after using the Fluo-8 AM fluorescence kit; ‘d’, the cells did not receive any treatment and after using the Fluo-8 AM fluorescence kit, the fluorescence curve of those cells was used as a negative control. Following exposure to HBSS (with CaCl2), the fluorescence curve of [Ca2+]I of the cells that TRPM7 silencing was higher than baseline, but lower than the cells that did not receive treatment. This indicated that TRPM7 silencing led to a significant decrease in the [Ca2+]I of the SKOV3 and OVCAR3 cells. ( b ) Fluorescent imaging the [Ca2+]i. SKOV3 and OVCAR3 cells were labeled with Fluo-8 AM and examined under a fluorescent microscope. Data are representative images or expressed as the mean ± SD of each group from three separate experiments
    Figure Legend Snippet: TRPM7 silencing reduces the intracellular calcium concentrations in ovarian cancer cells. ( a )The fluorescence curves of the SKOV3 and OVCAR3 cells. ‘a’, cells that did not receive any treatment, and were exposed to Ca2 + −free HANK’s solution after uploaded the Fluo-8 AM fluorescence kit. The fluorescence curve of those cells is used as baseline for [Ca2+]I; ‘b’, the cells were treated with MK886 for 24 h, and exposed to HBSS (with CaCl2) after using the Fluo-3 AM fluorescence kit; ‘c’, the cells did not receive any treatment, but were exposed to HBSS (with CaCl2) after using the Fluo-8 AM fluorescence kit; ‘d’, the cells did not receive any treatment and after using the Fluo-8 AM fluorescence kit, the fluorescence curve of those cells was used as a negative control. Following exposure to HBSS (with CaCl2), the fluorescence curve of [Ca2+]I of the cells that TRPM7 silencing was higher than baseline, but lower than the cells that did not receive treatment. This indicated that TRPM7 silencing led to a significant decrease in the [Ca2+]I of the SKOV3 and OVCAR3 cells. ( b ) Fluorescent imaging the [Ca2+]i. SKOV3 and OVCAR3 cells were labeled with Fluo-8 AM and examined under a fluorescent microscope. Data are representative images or expressed as the mean ± SD of each group from three separate experiments

    Techniques Used: Fluorescence, Negative Control, Imaging, Labeling, Microscopy

    6) Product Images from "Clinical and Practical Implications of Storage Media used for Tooth Avulsion"

    Article Title: Clinical and Practical Implications of Storage Media used for Tooth Avulsion

    Journal: International Journal of Clinical Pediatric Dentistry

    doi: 10.5005/jp-journals-10005-1427

    Hank’s balanced salt solution
    Figure Legend Snippet: Hank’s balanced salt solution

    Techniques Used:

    7) Product Images from "The secretory products of Trichomonas vaginalis decrease fertilizing capacity of mice sperm in vitro"

    Article Title: The secretory products of Trichomonas vaginalis decrease fertilizing capacity of mice sperm in vitro

    Journal: Asian Journal of Andrology

    doi: 10.4103/1008-682X.145070

    The effect of extracellular polymeric substances (EPS) of Trichomonas vaginalis on the viability of sperm. Viability was analyzed based on sperm morphology and vital staining with trypan blue. Data are presented at the mean ± standard deviation percent of viable sperm (relative to the total sperm numbers set at 100%) in three separate experiments, in which 200 cells were counted per treatment per experiment. EPS-10, -25, -50, -75, -100: sperm cells were incubated for 1, 2, or 3 h in 10%, 25%, 50%, 75%, or 100% of EPS prepared from T. vaginalis were diluted in Hank's balanced salt solution (HBSS). CT: control sperm cells were incubated at 37°C for 1, 2, or 3 h in HBSS that did not contain EPS.
    Figure Legend Snippet: The effect of extracellular polymeric substances (EPS) of Trichomonas vaginalis on the viability of sperm. Viability was analyzed based on sperm morphology and vital staining with trypan blue. Data are presented at the mean ± standard deviation percent of viable sperm (relative to the total sperm numbers set at 100%) in three separate experiments, in which 200 cells were counted per treatment per experiment. EPS-10, -25, -50, -75, -100: sperm cells were incubated for 1, 2, or 3 h in 10%, 25%, 50%, 75%, or 100% of EPS prepared from T. vaginalis were diluted in Hank's balanced salt solution (HBSS). CT: control sperm cells were incubated at 37°C for 1, 2, or 3 h in HBSS that did not contain EPS.

    Techniques Used: Staining, Standard Deviation, Incubation

    The effect of the EPS of Trichomonas vaginalis on the motility parameters of sperm. ( a ) The proportion of motile sperm with total motility after incubation for 2 h in 25%, 50%, or 75% of EPS of T. vaginalis diluted in Hank's balanced salt solution (HBSS). ( b ) The proportion of sperm with progressive motility after incubation for 2 h in 25%, 50%, or 75% EPS diluted in HBSS. Values are the mean ± standard deviation of three separate experiments; 200 cells were counted per treatment per experiment. Total motility: Grade A + B + C; Progressive motility: Grade A + B; Grade A: rapid progressive motility; Grade B: slow or sluggish progressive motility; Grade C: nonprogressive motility; Grade D: immotility; CT: control sperm cells were incubated at 37°C for 2 h in HBSS that did not contain EPS. EPS: extracellular polymeric substances prepared from T. vaginalis . * P
    Figure Legend Snippet: The effect of the EPS of Trichomonas vaginalis on the motility parameters of sperm. ( a ) The proportion of motile sperm with total motility after incubation for 2 h in 25%, 50%, or 75% of EPS of T. vaginalis diluted in Hank's balanced salt solution (HBSS). ( b ) The proportion of sperm with progressive motility after incubation for 2 h in 25%, 50%, or 75% EPS diluted in HBSS. Values are the mean ± standard deviation of three separate experiments; 200 cells were counted per treatment per experiment. Total motility: Grade A + B + C; Progressive motility: Grade A + B; Grade A: rapid progressive motility; Grade B: slow or sluggish progressive motility; Grade C: nonprogressive motility; Grade D: immotility; CT: control sperm cells were incubated at 37°C for 2 h in HBSS that did not contain EPS. EPS: extracellular polymeric substances prepared from T. vaginalis . * P

    Techniques Used: Incubation, Standard Deviation

    The effect of extracellular polymeric substances (EPS) of Trichomonas vaginalis on the proportion of sperm reacting positively to the hypo-osmotic swelling test. The percentage of swollen sperm was greatly reduced in sperm incubated with 75% EPS prepared from T. vaginalis . Sperm were incubated for 2 h in 25%, 50%, or 75% of EPS diluted in Hank's balanced salt solution (HBSS). CT: control sperm were incubated at 37°C for 2 h in HBSS that did not contain EPS. Values are the mean ± standard deviation of three separate experiments; 200 cells were counted per treatment per experiment. * P = 0.033 compared with the control.
    Figure Legend Snippet: The effect of extracellular polymeric substances (EPS) of Trichomonas vaginalis on the proportion of sperm reacting positively to the hypo-osmotic swelling test. The percentage of swollen sperm was greatly reduced in sperm incubated with 75% EPS prepared from T. vaginalis . Sperm were incubated for 2 h in 25%, 50%, or 75% of EPS diluted in Hank's balanced salt solution (HBSS). CT: control sperm were incubated at 37°C for 2 h in HBSS that did not contain EPS. Values are the mean ± standard deviation of three separate experiments; 200 cells were counted per treatment per experiment. * P = 0.033 compared with the control.

    Techniques Used: Incubation, Standard Deviation

    8) Product Images from "Protection From Lethal Lassa Disease Can Be Achieved Both Before and After Virus Exposure by Administration of Single-Cycle Replicating Lassa Virus Replicon Particles"

    Article Title: Protection From Lethal Lassa Disease Can Be Achieved Both Before and After Virus Exposure by Administration of Single-Cycle Replicating Lassa Virus Replicon Particles

    Journal: The Journal of infectious diseases

    doi: 10.1093/infdis/jiz284

    Refining the virus replicon particle vaccine genome and purification by ion exchange chromatography. (A) Lassa virus (LASV) replicon particles (VRPs) with empty GPC locus (Lassa VRP) were compared with VRPs that express the ZsGreen (ZSG) reporter from the GPC locus (Lassa VRP/ZSG). Virus-specific immunofluorescence assay (IFA) and ZSG fluorescence are shown. (B) Lassa VRPs and VRP/ZSG exhibit equivalent growth kinetics in Vero-LASV-GPCco cells, whereas VRPs in which the exonuclease has been inactivated by mutation (VRP/ZSG/ExoN) grow to a lower final titer. Vero-LASV-GPCco cells were inoculated at a multiplicity of infection of 0.05, and output was measured in Vero-E6 cells. (C) Schematic of VRP purification workflow. Raw supernatant was clarified by low-speed centrifugation and subjected to ion exchange chromatography. High-salt elution buffer was changed to physiological buffer, Hank’s balanced salt solution, using retention based on molecular mass of 100 kDa, and the resulting product was sterile-filtered using a low protein-binding membrane. Percentages represent remaining total infectivity compared with starting infectivity at designated steps; averages and standard deviations from 3 independent experiments; average and actual values from 2 independent experiments for ion exchange. ExoN, exonuclease-null; FFU, focus-forming units; PES, polyethersulfone.
    Figure Legend Snippet: Refining the virus replicon particle vaccine genome and purification by ion exchange chromatography. (A) Lassa virus (LASV) replicon particles (VRPs) with empty GPC locus (Lassa VRP) were compared with VRPs that express the ZsGreen (ZSG) reporter from the GPC locus (Lassa VRP/ZSG). Virus-specific immunofluorescence assay (IFA) and ZSG fluorescence are shown. (B) Lassa VRPs and VRP/ZSG exhibit equivalent growth kinetics in Vero-LASV-GPCco cells, whereas VRPs in which the exonuclease has been inactivated by mutation (VRP/ZSG/ExoN) grow to a lower final titer. Vero-LASV-GPCco cells were inoculated at a multiplicity of infection of 0.05, and output was measured in Vero-E6 cells. (C) Schematic of VRP purification workflow. Raw supernatant was clarified by low-speed centrifugation and subjected to ion exchange chromatography. High-salt elution buffer was changed to physiological buffer, Hank’s balanced salt solution, using retention based on molecular mass of 100 kDa, and the resulting product was sterile-filtered using a low protein-binding membrane. Percentages represent remaining total infectivity compared with starting infectivity at designated steps; averages and standard deviations from 3 independent experiments; average and actual values from 2 independent experiments for ion exchange. ExoN, exonuclease-null; FFU, focus-forming units; PES, polyethersulfone.

    Techniques Used: Refining, Purification, Ion Exchange Chromatography, Gel Permeation Chromatography, Immunofluorescence, Fluorescence, Mutagenesis, Infection, Centrifugation, Low Protein Binding

    9) Product Images from "In Vivo SPECT Reporter Gene Imaging of Regulatory T Cells"

    Article Title: In Vivo SPECT Reporter Gene Imaging of Regulatory T Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0025857

    Effect of 99m TcO 4 − uptake on the viability, phenotype and suppressive function of Tregs. A ) Standard MTT assay was performed using 1×10 6 B6s-NIS Tregs incubated in the presence of Hank's buffer supplemented with increasing 99m TcO 4 − (ranging from 0–100 MBq of 99m TcO 4 − ) in triplicates in a 96 well plate. B ) B6s-NIS Tregs were incubated with or without 99m TcO 4 − (30 MBq per million cells) and cultured in vitro for 7 days. These Tregs were then harvested for phenotypic and suppressive function. 5×10 5 Tregs were stained with anti-CD4-FITC, anti-CD25-PE and anti-FoxP3-APC. Plots shown here are gated on live cells using the forward and side scatter plots. C ) 99m TcO 4 − labelling had no effect on suppressive function of B6s-NIS Tregs. 5×10 4 freshly isolated CD4 + T cells were cultured alone, or with 5×10 4 transduced Tregs, in the presence of 1 µg/mL soluble purified anti-CD3ε and 1×10 5 APC. T cell proliferation was measured by [ 3 H]-thymidine incorporation (cpm) at 72 h of culture. *, P
    Figure Legend Snippet: Effect of 99m TcO 4 − uptake on the viability, phenotype and suppressive function of Tregs. A ) Standard MTT assay was performed using 1×10 6 B6s-NIS Tregs incubated in the presence of Hank's buffer supplemented with increasing 99m TcO 4 − (ranging from 0–100 MBq of 99m TcO 4 − ) in triplicates in a 96 well plate. B ) B6s-NIS Tregs were incubated with or without 99m TcO 4 − (30 MBq per million cells) and cultured in vitro for 7 days. These Tregs were then harvested for phenotypic and suppressive function. 5×10 5 Tregs were stained with anti-CD4-FITC, anti-CD25-PE and anti-FoxP3-APC. Plots shown here are gated on live cells using the forward and side scatter plots. C ) 99m TcO 4 − labelling had no effect on suppressive function of B6s-NIS Tregs. 5×10 4 freshly isolated CD4 + T cells were cultured alone, or with 5×10 4 transduced Tregs, in the presence of 1 µg/mL soluble purified anti-CD3ε and 1×10 5 APC. T cell proliferation was measured by [ 3 H]-thymidine incorporation (cpm) at 72 h of culture. *, P

    Techniques Used: MTT Assay, Incubation, Cell Culture, In Vitro, Staining, Isolation, Purification

    B6s-NIS Tregs uptake 99m TcO 4 − in vitro . 1×10 6 B6s or B6s-NIS Tregs were incubated in Hank's Balanced Salt solution supplemented with 1 MBq of 99m TcO 4 − . Sodium Perchlorate (100 µM) was used to block the 99m TcO 4 − uptake by B6s-NIS Treg line. **, P
    Figure Legend Snippet: B6s-NIS Tregs uptake 99m TcO 4 − in vitro . 1×10 6 B6s or B6s-NIS Tregs were incubated in Hank's Balanced Salt solution supplemented with 1 MBq of 99m TcO 4 − . Sodium Perchlorate (100 µM) was used to block the 99m TcO 4 − uptake by B6s-NIS Treg line. **, P

    Techniques Used: In Vitro, Incubation, Blocking Assay

    10) Product Images from "Knockdown of mitofilin inhibits autophagy and facilitates starvation-induced apoptosis in HeLa cells"

    Article Title: Knockdown of mitofilin inhibits autophagy and facilitates starvation-induced apoptosis in HeLa cells

    Journal: Iranian Journal of Basic Medical Sciences

    doi: 10.22038/ijbms.2019.36173.8617

    Mitofilin is down-regulated in starved HeLa cells. HeLa cells were cultured in Hank’s balanced salt solution for 2 hr. The cell lysate and total RNA were extracted and subjected to western blotting (A) and real-time PCR assay (B). β-actin was detected as a loading control in immunoblot. The mRNA level of mitofilin was normalized to the level of β-actin. Images are representative of three independent experiments. Data are expressed as mean±SD. * P
    Figure Legend Snippet: Mitofilin is down-regulated in starved HeLa cells. HeLa cells were cultured in Hank’s balanced salt solution for 2 hr. The cell lysate and total RNA were extracted and subjected to western blotting (A) and real-time PCR assay (B). β-actin was detected as a loading control in immunoblot. The mRNA level of mitofilin was normalized to the level of β-actin. Images are representative of three independent experiments. Data are expressed as mean±SD. * P

    Techniques Used: Cell Culture, Western Blot, Real-time Polymerase Chain Reaction

    11) Product Images from "Osteogenesis of osteogenic matrix cell sheets preserved in culture medium in a rat model"

    Article Title: Osteogenesis of osteogenic matrix cell sheets preserved in culture medium in a rat model

    Journal: Cell Transplantation

    doi: 10.1177/0963689718786233

    ATP content of OMCSs (A) and LDH activities (B) in the preservation solutions. There were significant differences in ATP content between the 22°C MEM and 4°C MEM groups, and 22°C HBSS and 4°C HBSS groups. For LDH activities, there were significant differences between the 22°C HBSS and 4°C HBSS groups. ATP: adenosine triphosphate; LDH: lactate dehydrogenase; MEM: minimum essential medium; HBSS: Hank’s balanced salt solution; ANOVA: analysis of variance
    Figure Legend Snippet: ATP content of OMCSs (A) and LDH activities (B) in the preservation solutions. There were significant differences in ATP content between the 22°C MEM and 4°C MEM groups, and 22°C HBSS and 4°C HBSS groups. For LDH activities, there were significant differences between the 22°C HBSS and 4°C HBSS groups. ATP: adenosine triphosphate; LDH: lactate dehydrogenase; MEM: minimum essential medium; HBSS: Hank’s balanced salt solution; ANOVA: analysis of variance

    Techniques Used: Preserving

    Hematoxylin and eosin-stained sections at 4 weeks after implantation into the subcutaneous layer of syngeneic rats. Poor bone formation was found in low magnification images of a β-tricalcium phosphate disk (TCP) in the 37°C MEM group (C and D). Low magnification images of a TCP disk in the 37°C HBSS group (E and F) showed negligible bone formation. Conversely, a high level of bone formation was visible in and around TCP in control (A and B), 22°C MEM (G and H), 22°C HBSS (I and J), 4°C MEM (K and L), and 4°C HBSS (M and N) groups. Asterisks indicate bone tissue. MEM: minimum essential medium; HBSS: Hank’s balanced salt solution
    Figure Legend Snippet: Hematoxylin and eosin-stained sections at 4 weeks after implantation into the subcutaneous layer of syngeneic rats. Poor bone formation was found in low magnification images of a β-tricalcium phosphate disk (TCP) in the 37°C MEM group (C and D). Low magnification images of a TCP disk in the 37°C HBSS group (E and F) showed negligible bone formation. Conversely, a high level of bone formation was visible in and around TCP in control (A and B), 22°C MEM (G and H), 22°C HBSS (I and J), 4°C MEM (K and L), and 4°C HBSS (M and N) groups. Asterisks indicate bone tissue. MEM: minimum essential medium; HBSS: Hank’s balanced salt solution

    Techniques Used: Staining

    ALP activity (A) and osteocalcin levels (B) in TCP constructs. ALP activities and osteocalcin levels in the 37°C HBSS group were significantly lower compared with those in the other groups. There was a significant difference in osteocalcin levels between 37°C MEM and 4°C HBSS groups. ALP: alkaline phosphatase; MEM: minimum essential medium; HBSS: Hank’s balanced salt solution; ANOVA: analysis of variance
    Figure Legend Snippet: ALP activity (A) and osteocalcin levels (B) in TCP constructs. ALP activities and osteocalcin levels in the 37°C HBSS group were significantly lower compared with those in the other groups. There was a significant difference in osteocalcin levels between 37°C MEM and 4°C HBSS groups. ALP: alkaline phosphatase; MEM: minimum essential medium; HBSS: Hank’s balanced salt solution; ANOVA: analysis of variance

    Techniques Used: ALP Assay, Activity Assay, Construct

    Related Articles

    Transfection:

    Article Title: Human P2Y11 Expression Level Affects Human P2X7 Receptor-Mediated Cell Death
    Article Snippet: .. Twenty-four hours after medium change, transfected cells were washed once in dye buffer prepared as Hank’s Balanced Salt Solution (HBSS), (#14170112, Gibco, Life Technology) with 0.2% YO-PRO-1® Iodide 491/509 (#Y3603, Life Technologies). .. Plates were read with NOVOStar (BMG LABTECH GmbH) microplate reader for every 2 min at 489/510 for 8 min before manually adding stimulatory compounds.

    Isolation:

    Article Title: Loss of Bcl-G, a Bcl-2 family member, augments the development of inflammation-associated colorectal cancer
    Article Snippet: .. Lamina propria cells were isolated from colon by incubation in 1 mM EDTA/Ca2+ Mg2+ free HBSS (ThermoFisher Scientific, 14170112) supplemented with 3% (v/v) FCS for 30 min at 37 °C with gentle shaking to remove epithelial cells. .. Colonic tissues were then minced and digested with 1 mg/ml of collagenase/dispase (Sigma–Aldrich, 11097113001) and 200μg/ml DNase I (Sigma–Aldrich, DN25-1G) in RPMI-1640 medium (ThermoFisher Scientific, 11875-093) supplemented with 3% (v/v) FCS for 45 mins at 37 °C with gentle shaking.

    Dissection:

    Article Title: Stress-induced phospho-ubiquitin formation causes parkin degradation
    Article Snippet: .. Cortices were harvested from E17-E18 rat embryonic brains in cold HBSS without calcium chloride, magnesium chloride, or magnesium sulfate (Thermo Fisher Scientific) using a dissection microscope. .. The cortices were dissociated by a 10-minute incubation in 0.05% trypsin at 37 °C and subsequent trituration in HBSS with two fire-polished pasteur pipettes of decreasing aperture size.

    Immunohistochemistry:

    Article Title: Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time
    Article Snippet: .. Intracranial Injection of AβpH , perfusion and immunohistochemistry Lyophilized AβpH was dissolved in Hank’s Balanced Salt Solution (HBSS no calcium, no magnesium, no phenol red, ThermoFisher #14175079) to obtain a 100 μ M stock solution that was then briefly vortexed and sonicated in a bath sonicator for 1 minute and directly used for intracranial injections or stored at −80°C. .. For intracranial injections, the stock solution was diluted in HBSS to obtain a 10 μ M working solution and kept on ice to prevent aggregation.

    Cell Culture:

    Article Title: Improvement of Aqueous Solubility of Lapatinib-derived Analogs: Identification of a Quinolinimine as a Lead for Human African Trypanosomiasis Drug Development
    Article Snippet: .. HepG2 cells cultured in complete MEM were first washed with 1× Hank’s Balanced Salt Solution (Invitrogen #14175095), trypsinized using a 0.25% trypsin/EDTA solution, assessed for viability using trypan blue, and resuspended at 250,000 cells/mL. .. Using a Tecan EVO Freedom robot, 38.3 μL of cell suspension were added to each well of clear, cell culture-treated 384-well microtiter plates for a final concentration of 9570 liver cells per well, and plated cells were incubated overnight in 5% CO2 at 37 °C.

    Incubation:

    Article Title: Loss of Bcl-G, a Bcl-2 family member, augments the development of inflammation-associated colorectal cancer
    Article Snippet: .. Lamina propria cells were isolated from colon by incubation in 1 mM EDTA/Ca2+ Mg2+ free HBSS (ThermoFisher Scientific, 14170112) supplemented with 3% (v/v) FCS for 30 min at 37 °C with gentle shaking to remove epithelial cells. .. Colonic tissues were then minced and digested with 1 mg/ml of collagenase/dispase (Sigma–Aldrich, 11097113001) and 200μg/ml DNase I (Sigma–Aldrich, DN25-1G) in RPMI-1640 medium (ThermoFisher Scientific, 11875-093) supplemented with 3% (v/v) FCS for 45 mins at 37 °C with gentle shaking.

    Microscopy:

    Article Title: Stress-induced phospho-ubiquitin formation causes parkin degradation
    Article Snippet: .. Cortices were harvested from E17-E18 rat embryonic brains in cold HBSS without calcium chloride, magnesium chloride, or magnesium sulfate (Thermo Fisher Scientific) using a dissection microscope. .. The cortices were dissociated by a 10-minute incubation in 0.05% trypsin at 37 °C and subsequent trituration in HBSS with two fire-polished pasteur pipettes of decreasing aperture size.

    FACS:

    Article Title: A transcriptional toolbox for exploring peripheral neuro-immune interactions
    Article Snippet: .. The digestion mix was subsequently removed and replaced with 100 μl of FACS buffer: HBSS (Gibco, Cat# 14175095) with 0.4% bovine serum albumin (Sigma-Aldrich Cat# A3983), 15 mM HEPES (Gibco Cat# 15630080) and 2 mM EDTA (Invitrogen Cat# 15575-038). .. Samples were dissociated by pipetting up and down 50x and passed through 70 μm Flowmi cell strainers (SP Scienceware, Cat# 136800070) into a 96 well v-bottom plate.

    Sonication:

    Article Title: Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time
    Article Snippet: .. Intracranial Injection of AβpH , perfusion and immunohistochemistry Lyophilized AβpH was dissolved in Hank’s Balanced Salt Solution (HBSS no calcium, no magnesium, no phenol red, ThermoFisher #14175079) to obtain a 100 μ M stock solution that was then briefly vortexed and sonicated in a bath sonicator for 1 minute and directly used for intracranial injections or stored at −80°C. .. For intracranial injections, the stock solution was diluted in HBSS to obtain a 10 μ M working solution and kept on ice to prevent aggregation.

    Injection:

    Article Title: Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time
    Article Snippet: .. Intracranial Injection of AβpH , perfusion and immunohistochemistry Lyophilized AβpH was dissolved in Hank’s Balanced Salt Solution (HBSS no calcium, no magnesium, no phenol red, ThermoFisher #14175079) to obtain a 100 μ M stock solution that was then briefly vortexed and sonicated in a bath sonicator for 1 minute and directly used for intracranial injections or stored at −80°C. .. For intracranial injections, the stock solution was diluted in HBSS to obtain a 10 μ M working solution and kept on ice to prevent aggregation.

    Staining:

    Article Title: Quantitative live-cell imaging yields novel insight into endogenous WNT/CTNNB1 signaling dynamics
    Article Snippet: .. For sorting, cells were stained with 1 µg/ml Dapi (D1306, Invitrogen) in HF (2 % FBS in HBSS (14175053, Gibco)), washed with HF and resuspended in HF. .. To determine the haploid population, a separate sample of cells was stained with 5 μM Vybrant® DyeCycleTM Violet Stain (V35003, Invitrogen) in full medium for 30 minutes and kept in vibrant containing medium.

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  • 93
    Thermo Fisher hank s balanced salt solution hbss
    Misoprostol pretreatment inhibits CXCL8-, LTB 4 -, and PAF-induced equine neutrophil chemotaxis. Calcein AM-labeled equine neutrophils were pretreated with multiple concentrations of misoprostol, dibutyryl cyclic-AMP (db-cAMP), or the vehicle control (VC) for each inhibitor <t>[Hank’s</t> balanced salt solution <t>(HBSS)].</t> The phosphatidylinositol-3 kinase (PI3K)-inhibitor wortmannin was used as a positive control for inhibition. Neutrophils were stimulated for 1 h with the following chemoattractants or vehicles: (A) 100 ng/ml CXCL8 (or HBSS vehicle), (B) 10 nM LTB 4 (or EtOH vehicle), or (C) 10 nM PAF (or EtOH vehicle). Percent chemotaxis was calculated by dividing florescence in each bottom well by 100% migration control wells. Data are expressed as mean% chemotaxis ± SEM. ** p
    Hank S Balanced Salt Solution Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hank s balanced salt solution hbss/product/Thermo Fisher
    Average 93 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    hank s balanced salt solution hbss - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    92
    Thermo Fisher hank s balanced salt solution
    Misoprostol pretreatment inhibits CXCL8-, LTB 4 -, and PAF-induced equine neutrophil chemotaxis. Calcein AM-labeled equine neutrophils were pretreated with multiple concentrations of misoprostol, dibutyryl cyclic-AMP (db-cAMP), or the vehicle control (VC) for each inhibitor <t>[Hank’s</t> balanced salt solution <t>(HBSS)].</t> The phosphatidylinositol-3 kinase (PI3K)-inhibitor wortmannin was used as a positive control for inhibition. Neutrophils were stimulated for 1 h with the following chemoattractants or vehicles: (A) 100 ng/ml CXCL8 (or HBSS vehicle), (B) 10 nM LTB 4 (or EtOH vehicle), or (C) 10 nM PAF (or EtOH vehicle). Percent chemotaxis was calculated by dividing florescence in each bottom well by 100% migration control wells. Data are expressed as mean% chemotaxis ± SEM. ** p
    Hank S Balanced Salt Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1048 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hank s balanced salt solution/product/Thermo Fisher
    Average 92 stars, based on 1048 article reviews
    Price from $9.99 to $1999.99
    hank s balanced salt solution - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Misoprostol pretreatment inhibits CXCL8-, LTB 4 -, and PAF-induced equine neutrophil chemotaxis. Calcein AM-labeled equine neutrophils were pretreated with multiple concentrations of misoprostol, dibutyryl cyclic-AMP (db-cAMP), or the vehicle control (VC) for each inhibitor [Hank’s balanced salt solution (HBSS)]. The phosphatidylinositol-3 kinase (PI3K)-inhibitor wortmannin was used as a positive control for inhibition. Neutrophils were stimulated for 1 h with the following chemoattractants or vehicles: (A) 100 ng/ml CXCL8 (or HBSS vehicle), (B) 10 nM LTB 4 (or EtOH vehicle), or (C) 10 nM PAF (or EtOH vehicle). Percent chemotaxis was calculated by dividing florescence in each bottom well by 100% migration control wells. Data are expressed as mean% chemotaxis ± SEM. ** p

    Journal: Frontiers in Veterinary Science

    Article Title: Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation

    doi: 10.3389/fvets.2017.00159

    Figure Lengend Snippet: Misoprostol pretreatment inhibits CXCL8-, LTB 4 -, and PAF-induced equine neutrophil chemotaxis. Calcein AM-labeled equine neutrophils were pretreated with multiple concentrations of misoprostol, dibutyryl cyclic-AMP (db-cAMP), or the vehicle control (VC) for each inhibitor [Hank’s balanced salt solution (HBSS)]. The phosphatidylinositol-3 kinase (PI3K)-inhibitor wortmannin was used as a positive control for inhibition. Neutrophils were stimulated for 1 h with the following chemoattractants or vehicles: (A) 100 ng/ml CXCL8 (or HBSS vehicle), (B) 10 nM LTB 4 (or EtOH vehicle), or (C) 10 nM PAF (or EtOH vehicle). Percent chemotaxis was calculated by dividing florescence in each bottom well by 100% migration control wells. Data are expressed as mean% chemotaxis ± SEM. ** p

    Article Snippet: Experimental Reagents Lipopolysaccharide (LPS) from E. coli 055:B5, phorbol 12-myristate 13-acetate (PMA), CXCL8, dibutyryl cyclic AMP (db-cAMP), wortmannin, staurosporine, bovine serum albumin (BSA), and anti-BSA antibody were from Sigma Aldrich (St. Louis, MO, USA); heat-inactivated fetal bovine serum (FBS) was from Gemini-Bioproducts (West Sacramento, CA, USA); misoprostol, LTB4 , and PAF were from Cayman Chemical (Ann Arbor, MI, USA); equine recombinant granulocyte-monocyte colony-stimulating factor (GM-CSF) was from Kingfisher Biotech (Saint Paul, MN, USA); and Hank’s balanced salt solution (HBSS) was from Thermo Fisher Scientific (Grand Island, NY, USA).

    Techniques: Chemotaxis Assay, Labeling, Positive Control, Inhibition, Migration