hamster immunoglobulin g  (Jackson Immuno)

 
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    Name:
    Biotin SP long spacer AffiniPure F ab ₂ Fragment Goat Anti Syrian Hamster IgG
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule syrian hamster IgG It also reacts with the light chains of other syrian hamster immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine horse human mouse rabbit and rat serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    107-066-142
    Price:
    157.0
    Category:
    F ab ₂ Fragment Affinity Purified Antibodies
    Conjugate:
    Biotin SP long spacer
    Size:
    0 5 ml
    Format:
    F(ab')₂ Fragment
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Jackson Immuno hamster immunoglobulin g
    Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes <t>(IgG2a,</t> <t>IgG2b,</t> and <t>IgG1)</t> were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule syrian hamster IgG It also reacts with the light chains of other syrian hamster immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine horse human mouse rabbit and rat serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/hamster immunoglobulin g/product/Jackson Immuno
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hamster immunoglobulin g - by Bioz Stars, 2021-06
    90/100 stars

    Images

    1) Product Images from "The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells"

    Article Title: The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20160579

    Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.
    Figure Legend Snippet: Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.

    Techniques Used: Mouse Assay, Functional Assay, Concentration Assay

    Related Articles

    Staining:

    Article Title: Antitumor efficacy of radiation plus immunotherapy depends upon dendritic cell activation of effector CDS+ T cells
    Article Snippet: Sodium azide was added to some wells to inhibit uptake and allow discrimination between tumor cells which have been truly internalized versus those which may be externally adhered to DC. .. Staining of CD11c (clone N418, AbD Serotec, U.K.) used a goat anti-hamster biotin-conjugated F(ab)2 (Jackson ImmunoResearch, U.K.) with amplification by the ABC method (Vector Lab, Peterborough, UK) and diaminobenzidine (DAB; Sigma, Poole, UK) visualization. .. Radiotherapy modulates the immunogenicity of tumor cells but is rarely able to initiate systemic antitumor responses.

    Amplification:

    Article Title: Antitumor efficacy of radiation plus immunotherapy depends upon dendritic cell activation of effector CDS+ T cells
    Article Snippet: Sodium azide was added to some wells to inhibit uptake and allow discrimination between tumor cells which have been truly internalized versus those which may be externally adhered to DC. .. Staining of CD11c (clone N418, AbD Serotec, U.K.) used a goat anti-hamster biotin-conjugated F(ab)2 (Jackson ImmunoResearch, U.K.) with amplification by the ABC method (Vector Lab, Peterborough, UK) and diaminobenzidine (DAB; Sigma, Poole, UK) visualization. .. Radiotherapy modulates the immunogenicity of tumor cells but is rarely able to initiate systemic antitumor responses.

    Incubation:

    Article Title: The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells
    Article Snippet: After 4 d of culture, B cell proliferation was evaluated by addition of 100 µl of CellTiter-Glo reagent (Promega). .. CD28 internalization assay Cells were incubated for 30 min on ice with anti-CD28 (1 µg/ml; 553294; BD), followed by incubation for another 30 min on ice with biotinylated goat antibody to hamster immunoglobulin G (2 µg/ml; 107–066-142; Jackson ImmunoResearch Laboratories). ..

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  • 90
    Jackson Immuno hamster immunoglobulin g
    Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes <t>(IgG2a,</t> <t>IgG2b,</t> and <t>IgG1)</t> were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.
    Hamster Immunoglobulin G, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hamster immunoglobulin g/product/Jackson Immuno
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hamster immunoglobulin g - by Bioz Stars, 2021-06
    90/100 stars
      Buy from Supplier

    92
    Jackson Immuno hamster immunoglobulin g igg
    The antiviral effect of IL-18 is mediated by IFN-γ and IFN-α/β. (Top Panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.32) were intraperitoneally injected with 250 μg of hamster MAbs to IFN-γ or TNF-α and sacrificed 24 h after administration of a single dose (10 μg) of IL-18. Control mice were simultaneously injected with 250 μg of irrelevant hamster <t>IgG</t> and sacrificed at the same time after IL-18 administration. Results were compared with those observed in livers from 2 age-, sex-, and serum HBeAg-matched transgenic littermates injected with saline (NaCl). Total hepatic DNA was analyzed for HBV DNA by Southern blot analysis as indicated. The sALT activity values at the time of autopsy are indicated for each mouse and expressed in units/liter. (Bottom panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.46) that were either heterozygous (+/−) or homozygous (−/−) for the IFN-α/β receptor null mutation were injected with 10 μg of IL-18, sacrificed 24 h later and their livers were processed as described above.
    Hamster Immunoglobulin G Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hamster immunoglobulin g igg/product/Jackson Immuno
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hamster immunoglobulin g igg - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    86
    Jackson Immuno control hamster polyclonal immunoglobulin ig g
    The antiviral effect of IL-18 is mediated by IFN-γ and IFN-α/β. (Top Panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.32) were intraperitoneally injected with 250 μg of hamster MAbs to IFN-γ or TNF-α and sacrificed 24 h after administration of a single dose (10 μg) of IL-18. Control mice were simultaneously injected with 250 μg of irrelevant hamster <t>IgG</t> and sacrificed at the same time after IL-18 administration. Results were compared with those observed in livers from 2 age-, sex-, and serum HBeAg-matched transgenic littermates injected with saline (NaCl). Total hepatic DNA was analyzed for HBV DNA by Southern blot analysis as indicated. The sALT activity values at the time of autopsy are indicated for each mouse and expressed in units/liter. (Bottom panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.46) that were either heterozygous (+/−) or homozygous (−/−) for the IFN-α/β receptor null mutation were injected with 10 μg of IL-18, sacrificed 24 h later and their livers were processed as described above.
    Control Hamster Polyclonal Immunoglobulin Ig G, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control hamster polyclonal immunoglobulin ig g/product/Jackson Immuno
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control hamster polyclonal immunoglobulin ig g - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.

    Journal: The Journal of Experimental Medicine

    Article Title: The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells

    doi: 10.1084/jem.20160579

    Figure Lengend Snippet: Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.

    Article Snippet: CD28 internalization assay Cells were incubated for 30 min on ice with anti-CD28 (1 µg/ml; 553294; BD), followed by incubation for another 30 min on ice with biotinylated goat antibody to hamster immunoglobulin G (2 µg/ml; 107–066-142; Jackson ImmunoResearch Laboratories).

    Techniques: Mouse Assay, Functional Assay, Concentration Assay

    The antiviral effect of IL-18 is mediated by IFN-γ and IFN-α/β. (Top Panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.32) were intraperitoneally injected with 250 μg of hamster MAbs to IFN-γ or TNF-α and sacrificed 24 h after administration of a single dose (10 μg) of IL-18. Control mice were simultaneously injected with 250 μg of irrelevant hamster IgG and sacrificed at the same time after IL-18 administration. Results were compared with those observed in livers from 2 age-, sex-, and serum HBeAg-matched transgenic littermates injected with saline (NaCl). Total hepatic DNA was analyzed for HBV DNA by Southern blot analysis as indicated. The sALT activity values at the time of autopsy are indicated for each mouse and expressed in units/liter. (Bottom panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.46) that were either heterozygous (+/−) or homozygous (−/−) for the IFN-α/β receptor null mutation were injected with 10 μg of IL-18, sacrificed 24 h later and their livers were processed as described above.

    Journal: Journal of Virology

    Article Title: Interleukin-18 Inhibits Hepatitis B Virus Replication in the Livers of Transgenic Mice †

    doi: 10.1128/JVI.76.21.10702-10707.2002

    Figure Lengend Snippet: The antiviral effect of IL-18 is mediated by IFN-γ and IFN-α/β. (Top Panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.32) were intraperitoneally injected with 250 μg of hamster MAbs to IFN-γ or TNF-α and sacrificed 24 h after administration of a single dose (10 μg) of IL-18. Control mice were simultaneously injected with 250 μg of irrelevant hamster IgG and sacrificed at the same time after IL-18 administration. Results were compared with those observed in livers from 2 age-, sex-, and serum HBeAg-matched transgenic littermates injected with saline (NaCl). Total hepatic DNA was analyzed for HBV DNA by Southern blot analysis as indicated. The sALT activity values at the time of autopsy are indicated for each mouse and expressed in units/liter. (Bottom panel) Age- and serum HBeAg-matched male transgenic mice (lineage 1.3.46) that were either heterozygous (+/−) or homozygous (−/−) for the IFN-α/β receptor null mutation were injected with 10 μg of IL-18, sacrificed 24 h later and their livers were processed as described above.

    Article Snippet: In those studies, purified hamster immunoglobulin G (IgG) (Jackson ImmunoResearch, West Grove, Pa.) was used as a control.

    Techniques: Transgenic Assay, Mouse Assay, Injection, Southern Blot, Activity Assay, Mutagenesis