halt protease (Thermo Fisher)
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 
86
|
Name:
Halt Protease Inhibitor Cocktail
Description:
Thermo Scientific Halt Protease Inhibitor Cocktail EDTA free 100X are ready to use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction Features of Halt Protease Inhibitor Single Use Cocktail EDTA free 100X • Convenient the refrigerator stable 100X liquid format is more effective and easier to use than individual inhibitors or other formats just pipette exactly the amount you need • No proprietary ingredients fully disclosed formulation contains optimized concentrations of six broad spectrum protease inhibitors AEBSF aprotinin bestatin E 64 leupeptin and pepstatin A stabilized in high quality dimethylsulfoxide DMSO • Multiple package sizes choose among several package sizes including single use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination • EDTA free formulations ensures compatibility with isoelectric focusing • Lysis buffer compatible use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent based lysis reagent Applications • Protection of intact active cellular proteins from degradation by endogenous proteases • Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents other commercially formulated cell or tissue lysis products sonication French press blender homogenization or repetitive freeze thaw cycling • Screening of extracts for proteolytic activity • The study of proteolysis in the regulation of cellular processes Add 10 µL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract The single use format is ideal for small scale cell lysis experiments where each 100 µL vial provides just what is needed for a 10 mL sample and there is no risk of contaminating or degrading remaining aliquots of cocktail Each microtube in the strip is heat sealed with a foil that can be easily punctured by a pipette tip for trouble free access to a precise amount of Halt Protease Inhibitor Cocktail needed for each experiment Proteases make up a large category of enzymes including endopeptidases and exopeptidases which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types Proteases are regulated and compartmentalized in living cells but cell lysis releases and mixes them with other extracted proteins Their activity can thus lead to the loss of a large number of valuable proteins adversely affecting downstream applications For this reason it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases Halt Protease Inhibitor Cocktails effectively inhibit serine proteases cysteine proteases aspartic acid proteases and aminopeptidases that are typically present in cellular lysate samples Regular and EDTA free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis respectively Related Products Halt Protease Inhibitor Single Use Cocktail 100X
Catalog Number:
78425
Price:
None
Category:
Lab Reagents and Chemicals
Applications:
Cell Lysis & Fractionation|Protease and Phosphatase Inhibition|Protein Biology|Protein Purification & Isolation
|
Buy from Supplier |
Structured Review
Thermo Fisher
halt protease
Thermo Scientific Halt Protease Inhibitor Cocktail EDTA free 100X are ready to use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction Features of Halt Protease Inhibitor Single Use Cocktail EDTA free 100X • Convenient the refrigerator stable 100X liquid format is more effective and easier to use than individual inhibitors or other formats just pipette exactly the amount you need • No proprietary ingredients fully disclosed formulation contains optimized concentrations of six broad spectrum protease inhibitors AEBSF aprotinin bestatin E 64 leupeptin and pepstatin A stabilized in high quality dimethylsulfoxide DMSO • Multiple package sizes choose among several package sizes including single use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination • EDTA free formulations ensures compatibility with isoelectric focusing • Lysis buffer compatible use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent based lysis reagent Applications • Protection of intact active cellular proteins from degradation by endogenous proteases • Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents other commercially formulated cell or tissue lysis products sonication French press blender homogenization or repetitive freeze thaw cycling • Screening of extracts for proteolytic activity • The study of proteolysis in the regulation of cellular processes Add 10 µL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract The single use format is ideal for small scale cell lysis experiments where each 100 µL vial provides just what is needed for a 10 mL sample and there is no risk of contaminating or degrading remaining aliquots of cocktail Each microtube in the strip is heat sealed with a foil that can be easily punctured by a pipette tip for trouble free access to a precise amount of Halt Protease Inhibitor Cocktail needed for each experiment Proteases make up a large category of enzymes including endopeptidases and exopeptidases which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types Proteases are regulated and compartmentalized in living cells but cell lysis releases and mixes them with other extracted proteins Their activity can thus lead to the loss of a large number of valuable proteins adversely affecting downstream applications For this reason it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases Halt Protease Inhibitor Cocktails effectively inhibit serine proteases cysteine proteases aspartic acid proteases and aminopeptidases that are typically present in cellular lysate samples Regular and EDTA free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis respectively Related Products Halt Protease Inhibitor Single Use Cocktail 100X
https://www.bioz.com/result/halt protease/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Thermo Scientific Halt Protease Inhibitor Cocktail EDTA free 100X are ready to use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction Features of Halt Protease Inhibitor Single Use Cocktail EDTA free 100X • Convenient the refrigerator stable 100X liquid format is more effective and easier to use than individual inhibitors or other formats just pipette exactly the amount you need • No proprietary ingredients fully disclosed formulation contains optimized concentrations of six broad spectrum protease inhibitors AEBSF aprotinin bestatin E 64 leupeptin and pepstatin A stabilized in high quality dimethylsulfoxide DMSO • Multiple package sizes choose among several package sizes including single use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination • EDTA free formulations ensures compatibility with isoelectric focusing • Lysis buffer compatible use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent based lysis reagent Applications • Protection of intact active cellular proteins from degradation by endogenous proteases • Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents other commercially formulated cell or tissue lysis products sonication French press blender homogenization or repetitive freeze thaw cycling • Screening of extracts for proteolytic activity • The study of proteolysis in the regulation of cellular processes Add 10 µL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract The single use format is ideal for small scale cell lysis experiments where each 100 µL vial provides just what is needed for a 10 mL sample and there is no risk of contaminating or degrading remaining aliquots of cocktail Each microtube in the strip is heat sealed with a foil that can be easily punctured by a pipette tip for trouble free access to a precise amount of Halt Protease Inhibitor Cocktail needed for each experiment Proteases make up a large category of enzymes including endopeptidases and exopeptidases which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types Proteases are regulated and compartmentalized in living cells but cell lysis releases and mixes them with other extracted proteins Their activity can thus lead to the loss of a large number of valuable proteins adversely affecting downstream applications For this reason it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases Halt Protease Inhibitor Cocktails effectively inhibit serine proteases cysteine proteases aspartic acid proteases and aminopeptidases that are typically present in cellular lysate samples Regular and EDTA free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis respectively Related Products Halt Protease Inhibitor Single Use Cocktail 100X
https://www.bioz.com/result/halt protease/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
halt protease - by Bioz Stars,
2021-03
86/100 stars
Images
Related Articles
Protease Inhibitor:Article Title: Characterization of the Canine Anthracycline-Metabolizing Enzyme Carbonyl Reductase 1 (cbr1) and the Functional Isoform cbr1 V218 Article Snippet: Bacterial cell pellets were harvested by centrifugation and stored at –80°C until further processing. .. Bacterial pellets were reconstituted and lysed in 3 ml of B-PER reagent (Thermo Fisher Scientific, Rockford, IL) supplemented with lysozyme, DNase I, and Article Title: The Redundancy of Peptidoglycan Carboxypeptidases Ensures Robust Cell Shape Maintenance in Escherichia coli Article Snippet: Cell growth was monitored until cultures reached an optical density at 600 nm (OD600 ) of 0.7 to 0.8, at which time the cells were harvested by centrifugation at 10,000 rpm for 10 min at 4°C, in a Sorvall benchtop centrifuge. .. The cell pellet was resuspended in 10 ml of filter-sterilized 25 mM citric acid-sodium phosphate buffer (pH 5.0 or pH 7.5, as required) containing Article Title: Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella Article Snippet: Quenched fluorescence protease activity assays Stock solutions (1.0 mM) of synthetic quenched fluorescence (QF) peptide substrates (GenScript Inc.) were dissolved in DMSO and working stocks (100 μM) were prepared using the molar extinction coefficient of the conjugated quencher, (2,4)-dinitrophenyl, of 6.985 cm–1 mM–1 at 400 nm. .. Purified recombinant FliA(H)-hypervariable region protease was incubated at a final concentration of 0.5 µM with 10 µM QF-peptide substrate in 100 µl of 150 mM NaCl, 10 mM CaCl2 , 50 mM HEPES, pH 7.5, in the presence of Article Title: Vasopressin type 1A receptor deletion enhances cardiac contractility, β-adrenergic receptor sensitivity and acute cardiac injury-induced dysfunction Article Snippet: A Tecan M1000 multimode plate reader was used to read the absorbance of the samples at 405 nm and Prism 5.0 software (GraphPad Software) was used to calculate the concentration of cAMP in the samples via interpolation from the linear regression of the standard curve. .. Left ventricular samples were homogenized in lysis buffer containing 20 mM Tris (pH 7.4), 137 mM NaCl, 10 % glycerol, 1 mM EDTA, 1 % NP-40, 10 mM NaF (Fisher Scientific), 1× Article Title: A System for Creating Stable Cell Lines that Express a Gene of Interest from a Bidirectional and Regulatable Herpes Simplex Virus Type 1 Promoter Article Snippet: .. Western immunoblotCells were removed from plates with trypsin, washed with PBS and proteins extracted using M-PER (Thermo Scientific) supplemented with Article Title: Expression and characterization of a codon-optimized alkaline-stable carbonic anhydrase from Aliivibrio salmonicida for CO2 sequestration applications. Article Snippet: The CO < sub > 2 < /sub > mineralization process, accelerated by carbonic anhydrase (CA) was proposed for the efficient capture and storage of CO < sub > 2 < /sub > , the accumulation of which in the atmosphere is the main cause of global warming. .. The CO < sub Concentration Assay:Article Title: The Redundancy of Peptidoglycan Carboxypeptidases Ensures Robust Cell Shape Maintenance in Escherichia coli Article Snippet: Cell growth was monitored until cultures reached an optical density at 600 nm (OD600 ) of 0.7 to 0.8, at which time the cells were harvested by centrifugation at 10,000 rpm for 10 min at 4°C, in a Sorvall benchtop centrifuge. .. The cell pellet was resuspended in 10 ml of filter-sterilized 25 mM citric acid-sodium phosphate buffer (pH 5.0 or pH 7.5, as required) containing Article Title: Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella Article Snippet: Quenched fluorescence protease activity assays Stock solutions (1.0 mM) of synthetic quenched fluorescence (QF) peptide substrates (GenScript Inc.) were dissolved in DMSO and working stocks (100 μM) were prepared using the molar extinction coefficient of the conjugated quencher, (2,4)-dinitrophenyl, of 6.985 cm–1 mM–1 at 400 nm. .. Purified recombinant FliA(H)-hypervariable region protease was incubated at a final concentration of 0.5 µM with 10 µM QF-peptide substrate in 100 µl of 150 mM NaCl, 10 mM CaCl2 , 50 mM HEPES, pH 7.5, in the presence of Purification:Article Title: Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella Article Snippet: Quenched fluorescence protease activity assays Stock solutions (1.0 mM) of synthetic quenched fluorescence (QF) peptide substrates (GenScript Inc.) were dissolved in DMSO and working stocks (100 μM) were prepared using the molar extinction coefficient of the conjugated quencher, (2,4)-dinitrophenyl, of 6.985 cm–1 mM–1 at 400 nm. .. Purified recombinant FliA(H)-hypervariable region protease was incubated at a final concentration of 0.5 µM with 10 µM QF-peptide substrate in 100 µl of 150 mM NaCl, 10 mM CaCl2 , 50 mM HEPES, pH 7.5, in the presence of Recombinant:Article Title: Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella Article Snippet: Quenched fluorescence protease activity assays Stock solutions (1.0 mM) of synthetic quenched fluorescence (QF) peptide substrates (GenScript Inc.) were dissolved in DMSO and working stocks (100 μM) were prepared using the molar extinction coefficient of the conjugated quencher, (2,4)-dinitrophenyl, of 6.985 cm–1 mM–1 at 400 nm. .. Purified recombinant FliA(H)-hypervariable region protease was incubated at a final concentration of 0.5 µM with 10 µM QF-peptide substrate in 100 µl of 150 mM NaCl, 10 mM CaCl2 , 50 mM HEPES, pH 7.5, in the presence of Incubation:Article Title: Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella Article Snippet: Quenched fluorescence protease activity assays Stock solutions (1.0 mM) of synthetic quenched fluorescence (QF) peptide substrates (GenScript Inc.) were dissolved in DMSO and working stocks (100 μM) were prepared using the molar extinction coefficient of the conjugated quencher, (2,4)-dinitrophenyl, of 6.985 cm–1 mM–1 at 400 nm. .. Purified recombinant FliA(H)-hypervariable region protease was incubated at a final concentration of 0.5 µM with 10 µM QF-peptide substrate in 100 µl of 150 mM NaCl, 10 mM CaCl2 , 50 mM HEPES, pH 7.5, in the presence of Protein Array:Article Title: Th1/M1 Conversion to Th2/M2 Responses in Models of Inflammation Lacking Cell Death Stimulates Maturation of Monocyte Precursors to Fibroblasts Article Snippet: Cells were analyzed on a Cell Lab Quanta SC flow cytometer (Beckman Coulter) using the Quanta Analysis software. .. Protein array After 3 days of I/R, hearts were harvested and protein was isolated using Cell Lysis Buffer (RayBiotech) supplemented with Isolation:Article Title: Th1/M1 Conversion to Th2/M2 Responses in Models of Inflammation Lacking Cell Death Stimulates Maturation of Monocyte Precursors to Fibroblasts Article Snippet: Cells were analyzed on a Cell Lab Quanta SC flow cytometer (Beckman Coulter) using the Quanta Analysis software. .. Protein array After 3 days of I/R, hearts were harvested and protein was isolated using Cell Lysis Buffer (RayBiotech) supplemented with Lysis:Article Title: Th1/M1 Conversion to Th2/M2 Responses in Models of Inflammation Lacking Cell Death Stimulates Maturation of Monocyte Precursors to Fibroblasts Article Snippet: Cells were analyzed on a Cell Lab Quanta SC flow cytometer (Beckman Coulter) using the Quanta Analysis software. .. Protein array After 3 days of I/R, hearts were harvested and protein was isolated using Cell Lysis Buffer (RayBiotech) supplemented with Article Title: Vasopressin type 1A receptor deletion enhances cardiac contractility, β-adrenergic receptor sensitivity and acute cardiac injury-induced dysfunction Article Snippet: A Tecan M1000 multimode plate reader was used to read the absorbance of the samples at 405 nm and Prism 5.0 software (GraphPad Software) was used to calculate the concentration of cAMP in the samples via interpolation from the linear regression of the standard curve. .. Left ventricular samples were homogenized in lysis buffer containing 20 mM Tris (pH 7.4), 137 mM NaCl, 10 % glycerol, 1 mM EDTA, 1 % NP-40, 10 mM NaF (Fisher Scientific), 1× Western Blot:Article Title: A System for Creating Stable Cell Lines that Express a Gene of Interest from a Bidirectional and Regulatable Herpes Simplex Virus Type 1 Promoter Article Snippet: .. Western immunoblotCells were removed from plates with trypsin, washed with PBS and proteins extracted using M-PER (Thermo Scientific) supplemented with |
