halt protease inhibitor mixture  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Halt Protease Inhibitor Cocktail 100X
    Description:
    Thermo Scientific Halt Protease Inhibitor Cocktail 100X are ready to use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction Features of Halt Protease Inhibitor Cocktail 100X • Convenient the refrigerator stable 100X liquid format is more effective and easier to use than individual inhibitors or other formats just pipette exactly the amount you need • No proprietary ingredients fully disclosed formulation contains optimized concentrations of six broad spectrum protease inhibitors AEBSF aprotinin bestatin E 64 leupeptin and pepstatin A stabilized in high quality dimethylsulfoxide DMSO • Multiple package sizes choose among several package sizes including single use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination • Regular formulation includes a separate vial of EDTA solution for optional metalloprotease inhibition • Lysis buffer compatible use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent based lysis reagent Add 10 µL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract Applications • Protection of intact active cellular proteins from degradation by endogenous proteases • Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents other commercially formulated cell or tissue lysis products sonication French press blender homogenization or repetitive freeze thaw cycling • Screening of extracts for proteolytic activity • The study of proteolysis in the regulation of cellular processes Proteases make up a large category of enzymes including endopeptidases and exopeptidases which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types Proteases are regulated and compartmentalized in living cells but cell lysis releases and mixes them with other extracted proteins Their activity can thus lead to the loss of a large number of valuable proteins adversely affecting downstream applications For this reason it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases Halt Protease Inhibitor Cocktails effectively inhibit serine proteases cysteine proteases aspartic acid proteases and aminopeptidases that are typically present in cellular lysate samples Regular and EDTA free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis respectively Related Products Halt Protease Inhibitor Single Use Cocktail EDTA free 100X
    Catalog Number:
    78429
    Price:
    None
    Applications:
    Cell Lysis & Fractionation|Protease and Phosphatase Inhibition|Protein Biology|Protein Purification & Isolation
    Category:
    Lab Reagents and Chemicals
    Buy from Supplier


    Structured Review

    Thermo Fisher halt protease inhibitor mixture
    Thermo Scientific Halt Protease Inhibitor Cocktail 100X are ready to use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction Features of Halt Protease Inhibitor Cocktail 100X • Convenient the refrigerator stable 100X liquid format is more effective and easier to use than individual inhibitors or other formats just pipette exactly the amount you need • No proprietary ingredients fully disclosed formulation contains optimized concentrations of six broad spectrum protease inhibitors AEBSF aprotinin bestatin E 64 leupeptin and pepstatin A stabilized in high quality dimethylsulfoxide DMSO • Multiple package sizes choose among several package sizes including single use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination • Regular formulation includes a separate vial of EDTA solution for optional metalloprotease inhibition • Lysis buffer compatible use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent based lysis reagent Add 10 µL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract Applications • Protection of intact active cellular proteins from degradation by endogenous proteases • Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents other commercially formulated cell or tissue lysis products sonication French press blender homogenization or repetitive freeze thaw cycling • Screening of extracts for proteolytic activity • The study of proteolysis in the regulation of cellular processes Proteases make up a large category of enzymes including endopeptidases and exopeptidases which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types Proteases are regulated and compartmentalized in living cells but cell lysis releases and mixes them with other extracted proteins Their activity can thus lead to the loss of a large number of valuable proteins adversely affecting downstream applications For this reason it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases Halt Protease Inhibitor Cocktails effectively inhibit serine proteases cysteine proteases aspartic acid proteases and aminopeptidases that are typically present in cellular lysate samples Regular and EDTA free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis respectively Related Products Halt Protease Inhibitor Single Use Cocktail EDTA free 100X
    https://www.bioz.com/result/halt protease inhibitor mixture/product/Thermo Fisher
    Average 99 stars, based on 116 article reviews
    Price from $9.99 to $1999.99
    halt protease inhibitor mixture - by Bioz Stars, 2020-11
    99/100 stars

    Images

    Related Articles

    Protease Inhibitor:

    Article Title: Comparative mouse lung injury by nickel nanoparticles with differential surface modification
    Article Snippet: .. Lung tissues were homogenized in ice-cold 1x PBS with 1× Halt™ Protease Inhibitor Cocktail (Thermo Scientific, Rockford, IL, USA) by using a Tissue-Tearor homogenizer (BioSpec Products, Bartlesville, OK, USA). ..

    Article Title: Lipoprotein LprI of Mycobacterium tuberculosis Acts as a Lysozyme Inhibitor *
    Article Snippet: .. M. smegmatis or M. tuberculosis (H37Ra) grown to midlog phase in Middlebrook 7H9 medium was collected after 10-min centrifugation at 2500 × g , washed, and resuspended in phosphate-buffered saline (PBS; 137 m m NaCl, 2.7 m m KCl, 10 m m , Na2 HPO4 , KH2 PO4 , pH 7.4) supplemented with 0.1% HaltTM protease inhibitor mixture (Thermo Scientific). ..

    Article Title: Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone
    Article Snippet: .. T98G cells were lysed on ice with RIPA Lysis, and Extraction Buffer Thermo Scientific™ (89900, Thermo Fisher, Waltham, MA, USA) supplemented with Halt™ Protease Inhibitor Cocktail, EDTA-free (100X) (78430, Thermo Fisher, Waltham, MA, USA). .. Protein content was estimated using the Pierce™ BCA Protein Assay Kit.

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: .. Frozen tumor samples were lysed with 500 µL ice cold lysis buffer plus Halt protease inhibitor (ThermoFisher #78430) using a Power Gen 125 tissue grinder (Fisher Scientific). .. Western blots were run as described in the supplemental material.

    Article Title: BET inhibition is an effective approach against KRAS-driven PDAC and NSCLC
    Article Snippet: .. Cells were lysed by resuspending the pellets in RIPA buffer containing Halt™ Protease Inhibitor Cocktail (Thermo) followed by a 30-minute incubation at 4°C and non-solubilized material was pelleted by centrifugation and discarded. .. Supernatants containing protein extracts were quantified by DC™ Protein Assay (Biorad) and equivalent protein concentrations dissolved in Laemmli buffer (containing 15% of β-mercaptoethanol).

    Centrifugation:

    Article Title: Lipoprotein LprI of Mycobacterium tuberculosis Acts as a Lysozyme Inhibitor *
    Article Snippet: .. M. smegmatis or M. tuberculosis (H37Ra) grown to midlog phase in Middlebrook 7H9 medium was collected after 10-min centrifugation at 2500 × g , washed, and resuspended in phosphate-buffered saline (PBS; 137 m m NaCl, 2.7 m m KCl, 10 m m , Na2 HPO4 , KH2 PO4 , pH 7.4) supplemented with 0.1% HaltTM protease inhibitor mixture (Thermo Scientific). ..

    Article Title: BET inhibition is an effective approach against KRAS-driven PDAC and NSCLC
    Article Snippet: .. Cells were lysed by resuspending the pellets in RIPA buffer containing Halt™ Protease Inhibitor Cocktail (Thermo) followed by a 30-minute incubation at 4°C and non-solubilized material was pelleted by centrifugation and discarded. .. Supernatants containing protein extracts were quantified by DC™ Protein Assay (Biorad) and equivalent protein concentrations dissolved in Laemmli buffer (containing 15% of β-mercaptoethanol).

    SDS Page:

    Article Title: The induction of autophagy by mechanical stress
    Article Snippet: .. The PBS and agarose sheet were then removed and the cells lysed in 200 μl RIPA buffer (10 mM Tris pH 7.4, 1 mM EDTA, 0.15 M NaCl, 1% NP40, 0.1% sodium deoxycholate, 0.1% SDS) supplemented with Halt protease cocktail (Pierce, 78430) and phosphatase inhibitor cocktail set 2 (Calbiochem, 524625), and analyzed by SDS-PAGE. .. All samples were separated on 4–12% NuPage Bis-Tris minigels (Invitrogen, NP0321), except LC3 lipidation assays, which were analyzed on large 15% gels.

    Incubation:

    Article Title: BET inhibition is an effective approach against KRAS-driven PDAC and NSCLC
    Article Snippet: .. Cells were lysed by resuspending the pellets in RIPA buffer containing Halt™ Protease Inhibitor Cocktail (Thermo) followed by a 30-minute incubation at 4°C and non-solubilized material was pelleted by centrifugation and discarded. .. Supernatants containing protein extracts were quantified by DC™ Protein Assay (Biorad) and equivalent protein concentrations dissolved in Laemmli buffer (containing 15% of β-mercaptoethanol).

    Lysis:

    Article Title: Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone
    Article Snippet: .. T98G cells were lysed on ice with RIPA Lysis, and Extraction Buffer Thermo Scientific™ (89900, Thermo Fisher, Waltham, MA, USA) supplemented with Halt™ Protease Inhibitor Cocktail, EDTA-free (100X) (78430, Thermo Fisher, Waltham, MA, USA). .. Protein content was estimated using the Pierce™ BCA Protein Assay Kit.

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: .. Frozen tumor samples were lysed with 500 µL ice cold lysis buffer plus Halt protease inhibitor (ThermoFisher #78430) using a Power Gen 125 tissue grinder (Fisher Scientific). .. Western blots were run as described in the supplemental material.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher phosphatase inhibitor mixture
    Phosphatase Inhibitor Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor mixture/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor mixture - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher halt protease inhibitor mixture
    Halt Protease Inhibitor Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/halt protease inhibitor mixture/product/Thermo Fisher
    Average 90 stars, based on 116 article reviews
    Price from $9.99 to $1999.99
    halt protease inhibitor mixture - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

    Image Search Results