halt protease and phosphatase inhibitor single use cocktail 100x  (Thermo Fisher)


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    Name:
    Halt Protease and Phosphatase Inhibitor Single Use Cocktail 100X
    Description:
    Thermo Scientific Halt Protease and Phosphatase Inhibitor Single Use Cocktail 100X provides the convenience of a single solution with full protein sample protection for cell and tissue lysates Features of Halt Protease and Phosphatase Inhibitor Single Use Cocktail 100X • Complete protection all in one cocktail contains both protease and phosphatase inhibitors • Multiple package sizes choose from four convenient packages sizes to match nearly any scale • Convenient the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats • Easy storage aqueous based format allows for convenient cold room storage without freezing by contrast with DMSO based cocktails • Compatible can be used in mass spectrometry MS because it does not contain AEBSF which can cause peaks to shift • Validated fully compatible with Thermo Scientific Pierce Cell Lysis Buffers Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification The cocktail contains inhibitors against the major classes of proteases and phosphatases targeting aminopeptidases cysteine and serine proteases and serine threonine and protein tyrosine phosphatases To promote the inhibition of metalloproteases 0 5M EDTA is provided in a separate tube Related Products Halt Protease and Phosphatase Inhibitor Cocktail EDTA free 100X
    Catalog Number:
    78442
    Price:
    None
    Category:
    Lab Reagents and Chemicals
    Applications:
    Cell Lysis & Fractionation|Protease and Phosphatase Inhibition|Protein Biology|Protein Purification & Isolation
    Buy from Supplier


    Structured Review

    Thermo Fisher halt protease and phosphatase inhibitor single use cocktail 100x
    4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP <t>100x</t> magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p
    Thermo Scientific Halt Protease and Phosphatase Inhibitor Single Use Cocktail 100X provides the convenience of a single solution with full protein sample protection for cell and tissue lysates Features of Halt Protease and Phosphatase Inhibitor Single Use Cocktail 100X • Complete protection all in one cocktail contains both protease and phosphatase inhibitors • Multiple package sizes choose from four convenient packages sizes to match nearly any scale • Convenient the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats • Easy storage aqueous based format allows for convenient cold room storage without freezing by contrast with DMSO based cocktails • Compatible can be used in mass spectrometry MS because it does not contain AEBSF which can cause peaks to shift • Validated fully compatible with Thermo Scientific Pierce Cell Lysis Buffers Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification The cocktail contains inhibitors against the major classes of proteases and phosphatases targeting aminopeptidases cysteine and serine proteases and serine threonine and protein tyrosine phosphatases To promote the inhibition of metalloproteases 0 5M EDTA is provided in a separate tube Related Products Halt Protease and Phosphatase Inhibitor Cocktail EDTA free 100X
    https://www.bioz.com/result/halt protease and phosphatase inhibitor single use cocktail 100x/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    halt protease and phosphatase inhibitor single use cocktail 100x - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen"

    Article Title: 4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen

    Journal: bioRxiv

    doi: 10.1101/2020.05.25.115097

    4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP 100x magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p
    Figure Legend Snippet: 4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP 100x magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p

    Techniques Used: Infection, Dot Blot, Western Blot, Immunohistochemistry, Staining

    2) Product Images from "4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen"

    Article Title: 4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen

    Journal: bioRxiv

    doi: 10.1101/2020.05.25.115097

    4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP 100x magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p
    Figure Legend Snippet: 4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP 100x magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p

    Techniques Used: Infection, Dot Blot, Western Blot, Immunohistochemistry, Staining

    Related Articles

    Infection:

    Article Title: 4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen
    Article Snippet: DNA sequencing was performed by Genewiz Incorporated. .. Bacterial infection dot blotTIB73 cells were lysed in whole cell lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100) with EDTA (1 µM) and Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, #78442). .. Protein concentration was determined using the Pierce BCA protein assay kit (Fisher Scientific, #PI23227).

    Lysis:

    Article Title: 4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen
    Article Snippet: DNA sequencing was performed by Genewiz Incorporated. .. Bacterial infection dot blotTIB73 cells were lysed in whole cell lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100) with EDTA (1 µM) and Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, #78442). .. Protein concentration was determined using the Pierce BCA protein assay kit (Fisher Scientific, #PI23227).

    Protease Inhibitor:

    Article Title: 4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen
    Article Snippet: DNA sequencing was performed by Genewiz Incorporated. .. Bacterial infection dot blotTIB73 cells were lysed in whole cell lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100) with EDTA (1 µM) and Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, #78442). .. Protein concentration was determined using the Pierce BCA protein assay kit (Fisher Scientific, #PI23227).

    other:

    Article Title: 4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen
    Article Snippet: DNA sequencing was performed by Genewiz Incorporated.

    Western Blot:

    Article Title: Cell–substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime
    Article Snippet: To examine the localization of labeled proteins in the pseudopods, cells were also imaged by AiryScan confocal microscope. .. Western blotting Dictyostelium cells were lysed by directly adding NuPAGE LDS sample buffer (Invitrogen) containing 20 mM DTT, HALT protease, and phosphatase inhibitors (Thermo Fisher Scientific) on top of moving cells and immediately boiling for 5 minutes. .. Mammalian cells were lysed with RIPA buffer, stored on ice for 10 minutes and debris cleared by centrifugation at 13,000 rpm, 5 minutes.

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  • 97
    Thermo Fisher halt protease and phosphatase inhibitor single use cocktail 100x
    4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP <t>100x</t> magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p
    Halt Protease And Phosphatase Inhibitor Single Use Cocktail 100x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/halt protease and phosphatase inhibitor single use cocktail 100x/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    halt protease and phosphatase inhibitor single use cocktail 100x - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP 100x magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p

    Journal: bioRxiv

    Article Title: 4-hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen

    doi: 10.1101/2020.05.25.115097

    Figure Lengend Snippet: 4-HNE accumulates during intracellular bacterial infection by L. monocytogenes . (A) 4-HNE accumulation in TIB73 murine hepatocytes during intracellular L. monocytogenes infection. 4-HNE adduct accumulation is assessed by dot blot whole cell Western and normalized to actin levels. Data are normalized 4-HNE levels as percent of 4-HNE at hour 0 of infection. Western image below is representative. (B) 4-HNE accumulation during 48-hour murine infection by GFP + L. monocytogenes assessed by immunohistochemistry analysis. Spleen (infected) anti-4-HNE. (C) spleen (uninfected) anti-4-HNE. (D) spleen (infected) anti-GFP 25x magnification. (E) spleen (infected) anti-4-HNE 25x magnification (F) spleen (infected) anti-GFP 100x magnification. (G) spleen (infected) anti-4-HNE 100x magnification. Antigens detected with 3,3-diaminobenzidine staining by horseradish peroxidase and cellular nuclei imaged with Hematoxylin counterstain. Data in (A) are pooled from two independent experiments. Statistics in (A) are an ordinary one-way ANOVA with a Dunnett’s multiple comparison test against hour 0. Error bars are mean +/- SD. *, p

    Article Snippet: Bacterial infection dot blot TIB73 cells were lysed in whole cell lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100) with EDTA (1 µM) and Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, #78442).

    Techniques: Infection, Dot Blot, Western Blot, Immunohistochemistry, Staining