Journal: Oncology Letters

Article Title: Knockdown of EBP1 promotes doxorubicin-induced apoptosis in renal clear cell carcinoma cells through activation of the p38/HIF-1α pathway

doi: 10.3892/ol.2025.14918

Figure Lengend Snippet: EBP1 is highly expressed in KIRC. (A) Analysis of EBP1 expression in KIRC and normal tissues using the GEPIA database. (B) Expression of EBP1 according to the pathological grade of KIRC assessed using UALCAN. (C) Expression of EBP1 according to the stage of KIRC assessed using GEPIA. (D) Association of EBP1 expression with the survival of patients with KIRC assessed using UALCAN. (E) Western blotting detection of lentiviral transduction efficiency in KIRC cell lines. Data are presented as the mean ± SD, n=3. ****P<0.0001 vs. sh-NC*1 and ++++ P<0.0001 vs. sh-NC*2. EBP1, ErbB3-binding protein; KIRC, kidney clear cell carcinoma; T, tumor; N, normal; TCGA, The Cancer Genome Atlas; Pr(>F), P-value for the F-statistic; sh-NC, short hairpin negative control; sh-EBP1, short hairpin EBP1.

Article Snippet: The 786-O and 769-P cells were transduced with two different short hairpin RNA (shRNA) lentiviral vectors targeting EBP1 (sh-EBP1*1 and sh-EBP1*2) and two different shRNA lentiviral vector negative controls (sh-NC*1 and sh-NC*2; Shanghai GeneChem Co., Ltd.).

Techniques: Expressing, Western Blot, Transduction, Binding Assay, Negative Control

Journal: Oncology Letters

Article Title: Knockdown of EBP1 promotes doxorubicin-induced apoptosis in renal clear cell carcinoma cells through activation of the p38/HIF-1α pathway

doi: 10.3892/ol.2025.14918

Figure Lengend Snippet: Knockdown of EBP1 decreases the viability and proliferation of doxorubicin-treated KIRC cells. (A) Cell Counting Kit-8 assay, (B) scratch assay (magnification, ×10), (C) colony formation assay and (D) TUNEL assay (scale bar, 50 µm) showing the effect of EBP1 knockdown on the proliferation, migration and apoptosis of KIRC cells treated with doxorubicin. Data are presented as the mean ± SD, n=3. *P<0.05, **P<0.01 and ***P<0.001 vs. sh-NC. EBP1, ErbB3-binding protein; KIRC, kidney clear cell carcinoma; Dox, doxorubicin; OD450 nm, optical density at 450 nm; sh-NC, short hairpin negative control; sh-EBP1, short hairpin EBP1.

Article Snippet: The 786-O and 769-P cells were transduced with two different short hairpin RNA (shRNA) lentiviral vectors targeting EBP1 (sh-EBP1*1 and sh-EBP1*2) and two different shRNA lentiviral vector negative controls (sh-NC*1 and sh-NC*2; Shanghai GeneChem Co., Ltd.).

Techniques: Knockdown, Cell Counting, Wound Healing Assay, Colony Assay, TUNEL Assay, Migration, Binding Assay, Negative Control

Journal: Oncology Letters

Article Title: Knockdown of EBP1 promotes doxorubicin-induced apoptosis in renal clear cell carcinoma cells through activation of the p38/HIF-1α pathway

doi: 10.3892/ol.2025.14918

Figure Lengend Snippet: Doxorubicin acts by inhibiting p38 phosphorylation and HIF-1α expression in kidney clear cell carcinoma cells. Effect of doxorubicin and SB203580 on p-p38 and HIF-1α protein levels in (A) 786-O cells and (B) 769-P cells. Representative western blots and quantitative analyses are shown, normalized to β-actin. Data are presented as the mean ± SD, n=3. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 vs. sh-NC; # P<0.05, ## P<0.01 and ### P<0.001 vs. sh-NC + Dox; + P<0.05, ++ P<0.01 and +++ P<0.001 vs. sh-NC + Dox + SB203580. p38, p38 mitogen-activated protein kinase; HIF-1α, hypoxia-inducible factor-1α; EBP1, ErbB3-binding protein; Dox, doxorubicin; sh-NC, short hairpin negative control; sh-EBP1, short hairpin EBP1; p-, phosphorylated.

Article Snippet: The 786-O and 769-P cells were transduced with two different short hairpin RNA (shRNA) lentiviral vectors targeting EBP1 (sh-EBP1*1 and sh-EBP1*2) and two different shRNA lentiviral vector negative controls (sh-NC*1 and sh-NC*2; Shanghai GeneChem Co., Ltd.).

Techniques: Expressing, Western Blot, Binding Assay, Negative Control

Journal: Oncology Letters

Article Title: Knockdown of EBP1 promotes doxorubicin-induced apoptosis in renal clear cell carcinoma cells through activation of the p38/HIF-1α pathway

doi: 10.3892/ol.2025.14918

Figure Lengend Snippet: Concurrent doxorubicin and EBP1 knockdown together promote apoptosis and reduce the viability of kidney clear cell carcinoma cells. Effect of doxorubicin on protein levels associated with apoptosis in (A) 786-O cells and (B) 769-P cells. Representative western blots and quantitative analyses are shown, normalized to β-actin. Effect of doxorubicin on (C) 786-O and (D) 769-P cell viability. Changes in viability over a 5-day period, measured daily, and a quantitative assessment on day 5 are shown. Data are presented as the mean ± SD, n=3. *P<0.05, **P<0.01 and ***P<0.001 vs. sh-NC; # P<0.05, ## P<0.01 and ### P<0.001 vs. sh-NC + Dox; + P<0.05, ++ P<0.01, +++ P<0.001 and ++++ P<0.0001 vs. sh-NC + Dox + SB203580. EBP1, ErbB3-binding protein; Dox, doxorubicin; sh-NC, short hairpin negative control; sh-EBP1, short hairpin EBP1.

Article Snippet: The 786-O and 769-P cells were transduced with two different short hairpin RNA (shRNA) lentiviral vectors targeting EBP1 (sh-EBP1*1 and sh-EBP1*2) and two different shRNA lentiviral vector negative controls (sh-NC*1 and sh-NC*2; Shanghai GeneChem Co., Ltd.).

Techniques: Knockdown, Western Blot, Binding Assay, Negative Control