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The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Gfp Ago2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/EGFP-hAgo2+(Plasmid+%2321981)/pmc13626668-190-0-7
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sgrnas in px003 puror  (Addgene inc)
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Addgene inc sgrnas in px003 puror
The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Sgrnas In Px003 Puror, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/EGFP-hAgo2+(Plasmid+%2321981)/pm41397992-258-28-31
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sgrnas in px003 puror - by Bioz Stars, 2026-10
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egfp hago2  (Addgene inc)
93
Addgene inc egfp hago2
The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Egfp Hago2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/EGFP-hAgo2+(Plasmid+%2321981)/pmc12477606-167-0-9
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phil sharp  (Addgene inc)
93
Addgene inc phil sharp
The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Phil Sharp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/EGFP-hAgo2+(Plasmid+%2321981)/pmc12477606-167-5-9
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phil sharp - by Bioz Stars, 2026-10
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expression plasmids egfp hago2  (Addgene inc)
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Addgene inc expression plasmids egfp hago2
The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Expression Plasmids Egfp Hago2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/EGFP-hAgo2+(Plasmid+%2321981)/pmc12272147-27-12-26
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expression plasmids egfp hago2 - by Bioz Stars, 2026-10
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human argonaute 2 protein hago2  (Sino Biological)
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Sino Biological human argonaute 2 protein hago2
The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Human Argonaute 2 Protein Hago2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/Human+AGO2+Argonaute+2+EIF2C2+Protein/pmc10107093__ANIE___62___0___s001-308-8-58
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human argonaute 2 protein hago2 - by Bioz Stars, 2026-10
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hago2  (Sino Biological)
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Sino Biological hago2
The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Hago2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/Human+AGO2+%2F+Argonaute+2+%2F+EIF2C2+Protein/pm39648859-72-33-34
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dynamics of guide-hago2 complexes  (Molecular Dynamics Inc)
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Molecular Dynamics Inc dynamics of guide-hago2 complexes
The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and <t>GFP‐Ago2.</t> Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.
Dynamics Of Guide Hago2 Complexes, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hago2/dynamics+of+guide+hago2+complexes/pm39490865-167-3-0
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Image Search Results


The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and GFP‐Ago2. Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.

Journal: Advanced Science

Article Title: The Cytoplasmic MicroRNA‐Production Complex Regulates MicroRNA Sorting to Extracellular Vesicles During Synaptogenesis

doi: 10.1002/advs.78067

Figure Lengend Snippet: The C‐terminal TRBP‐binding domain of Syncrip mediates its interaction with Ago2 and localization to P‐bodies. (A) HA‐Ago2 binds to GFP‐Syncrip‐WT and ‐∆N, but not ‐∆C. IP was performed in N2a cells using anti‐HA antibody. (B) Quantification of Syncrip‐containing puncta resembling RNA granules, n = 50–90 cells, **** p <0.0001, 1way ANOVA. (C) Syncrip granules overlap with Ago2‐containing RNA granules. N2a cells were transfected with mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, and GFP‐Ago2. Arrows point to overlapping puncta, DAPI shown in blue in merged images, scale bar; 5 µm. (D) Percentage of mCherry‐Syncrip puncta overlapping with GFP‐Ago2 granules. Line represents the mean, n = 47–90 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (E) Percentage of mCherry‐Syncrip puncta overlapping with the P‐body marker, GFP‐Dcp1a. Line represents the mean, n = 77–98 cells from 3 independent experiments, **** p <0.0001, Kruskal‐Wallis test. (F) Expression of mCherry‐Syncrip‐∆N and ‐∆C increases the number of GFP‐Ago2 puncta compared to WT in N2a cells; 85–90 cells from 3 independent experiments, **** p <0.0001, 1way ANOVA. (G) There are no differences in the number of GFP‐Dcp1a puncta in N2a cells expressing mCherry‐Syncrip‐WT, ‐∆N or ‐∆C, n = 77–98 cells from 3 independent experiments.

Article Snippet: GFP‐Ago2 was kindly provided by Phil Sharp (Addgene plasmid # 21981; RRID:Addgene_21981) [ ].

Techniques: Binding Assay, Transfection, Marker, Expressing

Endosome MCS regulate the localization of Syncrip and Ago2 to endosomes. (A) Airyscan images of 7DIV primary hippocampal neurons expressing mCherry‐ORP1L‐WT, ‐∆ORD or ‐∆ORDPHDPHD and GFP‐TRBP, and immunostained with Syncrip antibodies. Segmentation of selected ROI is shown, cherry‐ORP1L; red, GFP‐TRBP; green, Syncrip; blue, Syncrip‐TRBP co‐localization; white. (B) GFP‐TRBP and Syncrip co‐localization analysis in images acquired as in A, n = 18‐30, * p = 0.04, 1way ANOVA. (C) GFP‐PACT and Syncrip co‐localization analysis of Airyscan images of primary neurons expressing mCherry‐ORP1L‐WT, ‐∆ORD or ‐∆ORDPHDPHD, and GFP‐PACT, and immunostained with Syncrip antibodies, n = 18‐29. (D) Airyscan images of a 7DIV hippocampal neuron dendrite expressing mCherry‐ORP1L and GFP‐Ago2 and immunostained with Syncrip antibody. Arrow points to co‐localizing Ago2‐Syncrip puncta on the surface of ORP1L‐positive endosomes, scale bar: 5 µm. (E–G) Manders co‐localization coefficients between Ago2 (E) or Syncrip (F) with ORP1L, and between Ago2 and Syncrip (G) in dendrites of primary hippocampal neurons, n = 32‐48, * p = 0.01, ** p <0.01, *** p <0.001, **** p <0.0001.

Journal: Advanced Science

Article Title: The Cytoplasmic MicroRNA‐Production Complex Regulates MicroRNA Sorting to Extracellular Vesicles During Synaptogenesis

doi: 10.1002/advs.78067

Figure Lengend Snippet: Endosome MCS regulate the localization of Syncrip and Ago2 to endosomes. (A) Airyscan images of 7DIV primary hippocampal neurons expressing mCherry‐ORP1L‐WT, ‐∆ORD or ‐∆ORDPHDPHD and GFP‐TRBP, and immunostained with Syncrip antibodies. Segmentation of selected ROI is shown, cherry‐ORP1L; red, GFP‐TRBP; green, Syncrip; blue, Syncrip‐TRBP co‐localization; white. (B) GFP‐TRBP and Syncrip co‐localization analysis in images acquired as in A, n = 18‐30, * p = 0.04, 1way ANOVA. (C) GFP‐PACT and Syncrip co‐localization analysis of Airyscan images of primary neurons expressing mCherry‐ORP1L‐WT, ‐∆ORD or ‐∆ORDPHDPHD, and GFP‐PACT, and immunostained with Syncrip antibodies, n = 18‐29. (D) Airyscan images of a 7DIV hippocampal neuron dendrite expressing mCherry‐ORP1L and GFP‐Ago2 and immunostained with Syncrip antibody. Arrow points to co‐localizing Ago2‐Syncrip puncta on the surface of ORP1L‐positive endosomes, scale bar: 5 µm. (E–G) Manders co‐localization coefficients between Ago2 (E) or Syncrip (F) with ORP1L, and between Ago2 and Syncrip (G) in dendrites of primary hippocampal neurons, n = 32‐48, * p = 0.01, ** p <0.01, *** p <0.001, **** p <0.0001.

Article Snippet: GFP‐Ago2 was kindly provided by Phil Sharp (Addgene plasmid # 21981; RRID:Addgene_21981) [ ].

Techniques: Expressing, Analysis