reference strain atcc 19418  (ATCC)


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    ATCC reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reference strain atcc 19418 - by Bioz Stars, 2024-04
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    Images

    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    reference strain atcc 19418  (ATCC)


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    ATCC reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference strain atcc 19418/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reference strain atcc 19418 - by Bioz Stars, 2024-04
    99/100 stars

    Images

    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    influenzae reference strain  (ATCC)


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    ATCC influenzae reference strain
    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. <t>influenzae</t> reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.
    Influenzae Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.
    Figure Legend Snippet: Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.

    Techniques Used:

    Genbank reference strains.
    Figure Legend Snippet: Genbank reference strains.

    Techniques Used:

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).
    Figure Legend Snippet: The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).

    Techniques Used:

    PCR screening of respiratory reference isolates.
    Figure Legend Snippet: PCR screening of respiratory reference isolates.

    Techniques Used:

    influenzae reference strain atcc 19418  (ATCC)


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    ATCC influenzae reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenzae reference strain atcc 19418/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenzae reference strain atcc 19418 - by Bioz Stars, 2024-04
    99/100 stars

    Images

    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    haemophilus influenzae reference strain  (ATCC)


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    ATCC haemophilus influenzae reference strain
    Haemophilus Influenzae Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    additional reference strains nctc 11315  (ATCC)


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    ATCC additional reference strains nctc 11315
    Additional Reference Strains Nctc 11315, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    additional reference strains nctc 11315  (ATCC)


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    ATCC additional reference strains nctc 11315
    Additional Reference Strains Nctc 11315, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    influenzae 246 reference strain  (ATCC)


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    ATCC influenzae 246 reference strain
    Influenzae 246 Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reference strain mhb escherichia coli tol c mutant mhb haemophilus influenzae atcc 49247  (ATCC)


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    ATCC reference strain mhb escherichia coli tol c mutant mhb haemophilus influenzae atcc 49247
    Reference Strain Mhb Escherichia Coli Tol C Mutant Mhb Haemophilus Influenzae Atcc 49247, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mhb reference strain escherichia coli tol c mutant mhb haemophilus influenzae atcc 49247  (ATCC)


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    ATCC mhb reference strain escherichia coli tol c mutant mhb haemophilus influenzae atcc 49247
    Mhb Reference Strain Escherichia Coli Tol C Mutant Mhb Haemophilus Influenzae Atcc 49247, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    influenzae reference strain atcc 49247  (ATCC)


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    ATCC influenzae reference strain atcc 49247
    Influenzae Reference Strain Atcc 49247, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference strain atcc 19418/product/ATCC
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    ATCC influenzae reference strain
    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. <t>influenzae</t> reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.
    Influenzae Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC influenzae reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenzae reference strain atcc 19418/product/ATCC
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    ATCC haemophilus influenzae reference strain
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Haemophilus Influenzae Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC additional reference strains nctc 11315
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
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    ATCC influenzae 246 reference strain
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae 246 Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC reference strain mhb escherichia coli tol c mutant mhb haemophilus influenzae atcc 49247
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Reference Strain Mhb Escherichia Coli Tol C Mutant Mhb Haemophilus Influenzae Atcc 49247, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mhb reference strain escherichia coli tol c mutant mhb haemophilus influenzae atcc 49247
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Mhb Reference Strain Escherichia Coli Tol C Mutant Mhb Haemophilus Influenzae Atcc 49247, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC influenzae reference strain atcc 49247
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
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    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Article Snippet: DNA for standards was extracted from the reference strain ATCC 19418 and a dilution series ranging from 10 to 100000 genome copies per reaction was used to estimate the LOD of real time PCR assays.

    Techniques: Sequencing, Amplification

    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    Genbank reference strains.

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Genbank reference strains.

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques: Sequencing, Amplification

    The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    PCR screening of respiratory reference isolates.

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: PCR screening of respiratory reference isolates.

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Article Snippet: The upper group of curves included the H. influenzae reference strain ATCC 19418 and the lower group of curves included the Hh reference strain ATCC 33390.

    Techniques: Sequencing, Amplification