hadmsc cell culture normal hadmscs  (ATCC)


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    Name:
    Adipose Derived Mesenchymal Stem Cells Normal Human
    Description:
    Applications Adult stem cell differentiation regenerative medicine cell therapy tissue engineering creation of iPS cell lines Cell Type Mesenchymal Stem Host Homo sapiens human
    Catalog Number:
    PCS-500-011
    Price:
    None
    Applications:
    Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines
    Cell Type:
    Mesenchymal Stem
    Host:
    Homo sapiens, human
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    Structured Review

    ATCC hadmsc cell culture normal hadmscs
    <t>hADMSC</t> immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between <t>hADMSCs</t> and adipocytes was determined by Student's t -test, ∗ p
    Applications Adult stem cell differentiation regenerative medicine cell therapy tissue engineering creation of iPS cell lines Cell Type Mesenchymal Stem Host Homo sapiens human
    https://www.bioz.com/result/hadmsc cell culture normal hadmscs/product/ATCC
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hadmsc cell culture normal hadmscs - by Bioz Stars, 2021-06
    96/100 stars

    Images

    1) Product Images from "Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells"

    Article Title: Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

    Journal: Stem Cells International

    doi: 10.1155/2018/1615497

    hADMSC immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between hADMSCs and adipocytes was determined by Student's t -test, ∗ p
    Figure Legend Snippet: hADMSC immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between hADMSCs and adipocytes was determined by Student's t -test, ∗ p

    Techniques Used: Marker, Staining, Light Microscopy

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    Article Snippet: .. RNA-Sequencing of Cell Sheets Shows Significant Changes of MSC Transcriptome Profile Compared to Monolayer CultureFor RNA-sequencing experiments, an immortalized line of adipose-derived human MSC (ASCtelo52, ATCC, USA) was used to minimize donor-dependent variability. .. Assembly of CS was carried out in AdvanceStem medium as previously described [ ] and dense monolayer (90% confluent) cultured under the same conditions was used as a control.

    Cell Culture:

    Article Title: Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells
    Article Snippet: Physical characterization of the NP-antagomiRs was performed by Dynamic Light Scattering (Zetasizer, Malvern), Zeta Potential (Zetasizer, Malvern), UV/Vis Spectroscopy and Transmission Electron Microscopy (see and ). .. Cell culture: Osteosarcoma cell line (MG63s) and primary stem cells (MSCs) The human osteosarcoma cell line MG63 (ATCC® CRL-1427™ ) was employed in this study because of its innately high levels of miR-31. .. MG63s were expanded in Dulbecco’s Modified Eagle Medium (DMEM) growth medium with 10% FBS and 1% penicillin streptomycin and maintained at 37°C in 5% CO2 until ~90% confluent.

    Article Title: Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
    Article Snippet: .. 2.1. hADMSC Cell Culture Normal hADMSCs (ATCC® PCS-500-011™) were cultured at an early passage (passage 4). .. Cell cultures were made using Gibco MesenPRO RS™ Medium (cat. number 12746-012) prepared according to the supplier's specifications and incubated at 37°C and 5% CO2 .

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
    Article Snippet: In the present study, we investigated the effect of chitosan-conjugated AuNPs on the differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) as a switch to determine cell fate into osteoblasts and the relating signaling pathway in cell differentiation. .. Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 . ..

    Expressing:

    Article Title: Collaboration of cancer‐associated fibroblasts and tumour‐associated macrophages for neuroblastoma development
    Article Snippet: NBCM induced CAF markers in human BM‐MSCs We next investigated whether 50% NBCM would induce the expression of CAF markers in human neonate dermal fibroblasts and human BM‐MSCs as examples of stromal cells. .. Although expression of the CAF marker αSMA (ACTA2 ) was not enhanced in the human neonate dermal fibroblasts (Figure A), the human BM‐MSCs showed increased αSMA and FAP mRNA expression when in 50% NBCM, in a time‐dependent manner (Figure B). .. We also confirmed that αSMA and FAP proteins were induced in human BM‐MSCs cultured in 50% NBCM for 7 days (Figure C, D).

    Marker:

    Article Title: Collaboration of cancer‐associated fibroblasts and tumour‐associated macrophages for neuroblastoma development
    Article Snippet: NBCM induced CAF markers in human BM‐MSCs We next investigated whether 50% NBCM would induce the expression of CAF markers in human neonate dermal fibroblasts and human BM‐MSCs as examples of stromal cells. .. Although expression of the CAF marker αSMA (ACTA2 ) was not enhanced in the human neonate dermal fibroblasts (Figure A), the human BM‐MSCs showed increased αSMA and FAP mRNA expression when in 50% NBCM, in a time‐dependent manner (Figure B). .. We also confirmed that αSMA and FAP proteins were induced in human BM‐MSCs cultured in 50% NBCM for 7 days (Figure C, D).

    Modification:

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
    Article Snippet: In the present study, we investigated the effect of chitosan-conjugated AuNPs on the differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) as a switch to determine cell fate into osteoblasts and the relating signaling pathway in cell differentiation. .. Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 . ..

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    ATCC hadmsc cell culture normal hadmscs
    <t>hADMSC</t> immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between <t>hADMSCs</t> and adipocytes was determined by Student's t -test, ∗ p
    Hadmsc Cell Culture Normal Hadmscs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hadmsc cell culture normal hadmscs/product/ATCC
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hadmsc cell culture normal hadmscs - by Bioz Stars, 2021-06
    96/100 stars
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    Image Search Results


    hADMSC immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between hADMSCs and adipocytes was determined by Student's t -test, ∗ p

    Journal: Stem Cells International

    Article Title: Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

    doi: 10.1155/2018/1615497

    Figure Lengend Snippet: hADMSC immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between hADMSCs and adipocytes was determined by Student's t -test, ∗ p

    Article Snippet: 2.1. hADMSC Cell Culture Normal hADMSCs (ATCC® PCS-500-011™) were cultured at an early passage (passage 4).

    Techniques: Marker, Staining, Light Microscopy

    Effects of chitosan-conjugated AuNPs. Notes: ( A ) On the cell viability of hADMSCs for 72 hours in a growth medium (GM) and ( B ) on the cell proliferation of hADMSCs for 10, 14, and 21 days after the induction of osteogenic differentiation. Results are mean ± SE of the triplicate experiments. * P

    Journal: International Journal of Nanomedicine

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

    doi: 10.2147/IJN.S78775

    Figure Lengend Snippet: Effects of chitosan-conjugated AuNPs. Notes: ( A ) On the cell viability of hADMSCs for 72 hours in a growth medium (GM) and ( B ) on the cell proliferation of hADMSCs for 10, 14, and 21 days after the induction of osteogenic differentiation. Results are mean ± SE of the triplicate experiments. * P

    Article Snippet: Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 .

    Techniques:

    TEM images of hADMSCs exposed to chitosan-conjugated AuNPs. Notes: hADMSCs were exposed to chitosan-conjugated AuNPs for 24 hours and then fixed for TEM. ( A ) Chitosan-conjugated AuNPs were internalized in cell membrane as magnified in the ( B ) image. White arrows represent chitosan-conjugated AuNPs in cell membrane. The scale bars in ( A ) and ( B ) are 0.5 µm and 100 nm. Abbreviations: AuNPs, gold nanoparticles; hADMSCs, human adipose-derived mesenchymal stem cells; TEM, transmission electron microscope.

    Journal: International Journal of Nanomedicine

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

    doi: 10.2147/IJN.S78775

    Figure Lengend Snippet: TEM images of hADMSCs exposed to chitosan-conjugated AuNPs. Notes: hADMSCs were exposed to chitosan-conjugated AuNPs for 24 hours and then fixed for TEM. ( A ) Chitosan-conjugated AuNPs were internalized in cell membrane as magnified in the ( B ) image. White arrows represent chitosan-conjugated AuNPs in cell membrane. The scale bars in ( A ) and ( B ) are 0.5 µm and 100 nm. Abbreviations: AuNPs, gold nanoparticles; hADMSCs, human adipose-derived mesenchymal stem cells; TEM, transmission electron microscope.

    Article Snippet: Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 .

    Techniques: Transmission Electron Microscopy, Derivative Assay, Transmission Assay, Microscopy

    Effect of chitosan-conjugated AuNPs on adipogenic differentiation. Notes: Lipid accumulation of hADMSCs treated with chitosan-conjugated AuNPs after 10, 14, and 21 days of culturing. Results are mean ± SE of the triplicate experiments. Abbreviations: AuNPs, gold nanoparticles; GM, growth medium; hADMSCs, human adipose-derived mesenchymal stem cells; OM, osteogenic-inducing medium; SE, standard error.

    Journal: International Journal of Nanomedicine

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

    doi: 10.2147/IJN.S78775

    Figure Lengend Snippet: Effect of chitosan-conjugated AuNPs on adipogenic differentiation. Notes: Lipid accumulation of hADMSCs treated with chitosan-conjugated AuNPs after 10, 14, and 21 days of culturing. Results are mean ± SE of the triplicate experiments. Abbreviations: AuNPs, gold nanoparticles; GM, growth medium; hADMSCs, human adipose-derived mesenchymal stem cells; OM, osteogenic-inducing medium; SE, standard error.

    Article Snippet: Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 .

    Techniques: Derivative Assay

    A real-time RT-PCR analysis of the osteogenic marker gene expression in hADMSCs treated with chitosan-conjugated AuNPs for 10, 14, and 21 days. Notes: ( A ) Alkaline phosphatase (ALP); ( B ) bone sialoprotein (BSP); ( C ) osteocalcin (OSC). Results are mean ± SE of the triplicate experiments. * P

    Journal: International Journal of Nanomedicine

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

    doi: 10.2147/IJN.S78775

    Figure Lengend Snippet: A real-time RT-PCR analysis of the osteogenic marker gene expression in hADMSCs treated with chitosan-conjugated AuNPs for 10, 14, and 21 days. Notes: ( A ) Alkaline phosphatase (ALP); ( B ) bone sialoprotein (BSP); ( C ) osteocalcin (OSC). Results are mean ± SE of the triplicate experiments. * P

    Article Snippet: Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 .

    Techniques: Quantitative RT-PCR, Marker, Expressing, ALP Assay

    Effect of chitosan-conjugated AuNPs on the mineralization of hADMSCs. Notes: ( A ) Stained calcium deposition by Alizarin Red S. ( B ) Mineralization was quantitated through the elution of Alizarin Red S from stained mineral deposits. Results are mean ± SE of the triplicate experiments, * P

    Journal: International Journal of Nanomedicine

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

    doi: 10.2147/IJN.S78775

    Figure Lengend Snippet: Effect of chitosan-conjugated AuNPs on the mineralization of hADMSCs. Notes: ( A ) Stained calcium deposition by Alizarin Red S. ( B ) Mineralization was quantitated through the elution of Alizarin Red S from stained mineral deposits. Results are mean ± SE of the triplicate experiments, * P

    Article Snippet: Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 .

    Techniques: Staining

    Activation of the osteogenic signaling pathway by chitosan-conjugated AuNPs. Notes: ( A ) A western blot analysis of the nonphosphorylated β-catenin protein expression after treatment with chitosan-conjugated AuNPs for 7 days. ( B ) Translocation of nonphosphorylated β-catenin into the nuclei of hADMSCs treated with chitosan-conjugated AuNPs for 7, 10, 14, and 21 days. Abbreviations: AuNPs, gold nanoparticles; GM, growth medium; hADMSCs, human adipose-derived mesenchymal stem cells; OM, osteogenic-inducing medium.

    Journal: International Journal of Nanomedicine

    Article Title: Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

    doi: 10.2147/IJN.S78775

    Figure Lengend Snippet: Activation of the osteogenic signaling pathway by chitosan-conjugated AuNPs. Notes: ( A ) A western blot analysis of the nonphosphorylated β-catenin protein expression after treatment with chitosan-conjugated AuNPs for 7 days. ( B ) Translocation of nonphosphorylated β-catenin into the nuclei of hADMSCs treated with chitosan-conjugated AuNPs for 7, 10, 14, and 21 days. Abbreviations: AuNPs, gold nanoparticles; GM, growth medium; hADMSCs, human adipose-derived mesenchymal stem cells; OM, osteogenic-inducing medium.

    Article Snippet: Culture of hADMSCs The hADMSCs were purchased from ATCC (PCS-500-011) and cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (GIBCO cell culture, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum and 1% antibiotics (Sigma-Aldrich, St Louis, MO, USA) incubating at 37°C in 5% CO2 .

    Techniques: Activation Assay, Western Blot, Expressing, Translocation Assay, Derivative Assay

    DNA damage accumulation through adipocyte differentiation of hADMSCs. DNA damage corresponding to DNA breaks and alkali labile sites accumulated over 14 days of adipocyte differentiation was expressed as Olive tail moment (OTM), (a) (mean ± SD). DNA oxidative damage accumulation, corresponding to DNA-Fpg sites (7,8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil) expressed as OTM, is represented in (b) (mean ± SD). Basal DNA damage (breaks + alkali labile sites) and basal DNA oxidative damage (Fpg-sensitive sites) of hADMSCs (U = undifferentiated) and adipocytes (D = differentiated) are represented in categories of damage (0, without damage to 4, highest damage) in (c). ROS intracellular levels in hADMSCs and adipocytes are shown in (d) (mean ± SD). Data represent 3 or 4 independent experiments with duplicates. Nonparametric tests were used for single cell gel electrophoresis analyses, Kruskal-Wallis (one-way ANOVA on ranks), and all pairwise multiple comparison procedures (Dunn's test). Differences versus hADMSCs (undifferentiated), ∗ p .

    Journal: Stem Cells International

    Article Title: Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

    doi: 10.1155/2018/1615497

    Figure Lengend Snippet: DNA damage accumulation through adipocyte differentiation of hADMSCs. DNA damage corresponding to DNA breaks and alkali labile sites accumulated over 14 days of adipocyte differentiation was expressed as Olive tail moment (OTM), (a) (mean ± SD). DNA oxidative damage accumulation, corresponding to DNA-Fpg sites (7,8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil) expressed as OTM, is represented in (b) (mean ± SD). Basal DNA damage (breaks + alkali labile sites) and basal DNA oxidative damage (Fpg-sensitive sites) of hADMSCs (U = undifferentiated) and adipocytes (D = differentiated) are represented in categories of damage (0, without damage to 4, highest damage) in (c). ROS intracellular levels in hADMSCs and adipocytes are shown in (d) (mean ± SD). Data represent 3 or 4 independent experiments with duplicates. Nonparametric tests were used for single cell gel electrophoresis analyses, Kruskal-Wallis (one-way ANOVA on ranks), and all pairwise multiple comparison procedures (Dunn's test). Differences versus hADMSCs (undifferentiated), ∗ p .

    Article Snippet: Normal hADMSCs (ATCC® PCS-500-011™) were cultured at an early passage (passage 4).

    Techniques: Single Cell Gel Electrophoresis

    hADMSC immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between hADMSCs and adipocytes was determined by Student's t -test, ∗ p

    Journal: Stem Cells International

    Article Title: Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

    doi: 10.1155/2018/1615497

    Figure Lengend Snippet: hADMSC immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between hADMSCs and adipocytes was determined by Student's t -test, ∗ p

    Article Snippet: Normal hADMSCs (ATCC® PCS-500-011™) were cultured at an early passage (passage 4).

    Techniques: Marker, Staining, Light Microscopy