ha2 recombinant protein  (Sino Biological)


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    Structured Review

    Sino Biological ha2 recombinant protein
    Ha2 Recombinant Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ha2 recombinant protein/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ha2 recombinant protein - by Bioz Stars, 2021-07
    86/100 stars

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    Related Articles

    SDS Page:

    Article Title: In Vitro and In Vivo Antiviral Activity of Nylidrin by Targeting the Hemagglutinin 2-Mediated Membrane Fusion of Influenza A Virus
    Article Snippet: .. After 12% SDS-PAGE and electrotransfer, both HA1 and HA2 proteins were probed with rabbit anti-HA1 (cat no. 11684-T54; Sino Biological) and rabbit anti-HA2 antibodies (cat no. 86001-RM02; Sino Biological), respectively, followed by incubation with HRP-conjugated goat anti-rabbit secondary antibody as described above. .. In Vivo Study Female BALB/c mice (6–7 weeks old; Orient Bio Inc., Gyeonggi-do, Republic Korea) were infected with mouse-adapted PR8 (maPR8).

    Electrotransfer:

    Article Title: In Vitro and In Vivo Antiviral Activity of Nylidrin by Targeting the Hemagglutinin 2-Mediated Membrane Fusion of Influenza A Virus
    Article Snippet: .. After 12% SDS-PAGE and electrotransfer, both HA1 and HA2 proteins were probed with rabbit anti-HA1 (cat no. 11684-T54; Sino Biological) and rabbit anti-HA2 antibodies (cat no. 86001-RM02; Sino Biological), respectively, followed by incubation with HRP-conjugated goat anti-rabbit secondary antibody as described above. .. In Vivo Study Female BALB/c mice (6–7 weeks old; Orient Bio Inc., Gyeonggi-do, Republic Korea) were infected with mouse-adapted PR8 (maPR8).

    Incubation:

    Article Title: In Vitro and In Vivo Antiviral Activity of Nylidrin by Targeting the Hemagglutinin 2-Mediated Membrane Fusion of Influenza A Virus
    Article Snippet: .. After 12% SDS-PAGE and electrotransfer, both HA1 and HA2 proteins were probed with rabbit anti-HA1 (cat no. 11684-T54; Sino Biological) and rabbit anti-HA2 antibodies (cat no. 86001-RM02; Sino Biological), respectively, followed by incubation with HRP-conjugated goat anti-rabbit secondary antibody as described above. .. In Vivo Study Female BALB/c mice (6–7 weeks old; Orient Bio Inc., Gyeonggi-do, Republic Korea) were infected with mouse-adapted PR8 (maPR8).

    Article Title: Salinomycin Inhibits Influenza Virus Infection by Disrupting Endosomal Acidification and Viral Matrix Protein 2 Function
    Article Snippet: Vero E6 cells in a 12-well plate (3 × 105 cells/ml) were infected with PR8 at an MOI of 0.5 at 37°C without TPCK-trypsin. .. On the next day, virus-infected cells were stimulated with TPCK-trypsin (5 μg/ml) in DMEM at 37°C for 15 min and then incubated with 2 μM salinomycin or an anti-HA2 antibody (0.5 or 5.0 μg/ml; Sino Biological) in DMEM for additional 15 min. After washing with PBS containing 1 mM MgCl2 and 0.1 mM CaCl2 (PBS-CM), the cells were treated again with each compound dissolved in acidic or neutral PBS-CM (pH 5.6 or 7.0, respectively) for 15 min, for which the pH was adjusted with citric acid. ..

    other:

    Article Title: Junctional and somatic hypermutation-induced CX4C motif is critical for the recognition of a highly conserved epitope on HCV E2 by a human broadly neutralizing antibody
    Article Snippet: The human mAb 3E1 against HA2 of influenza H1N1 was used as an isotype control.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Influenza virus infection expands the breadth of antibody responses through IL-4 signalling in B cells
    Article Snippet: ELISA of LAH peptide and mini-HA, A/Narita/1/2009 HA 420-474 and recombinant HA2 (rHA2) were used as LAH and mini-HA antigens. .. IgG titers for the nuclear protein of influenza virus were measured by ELISA using His-conjugated recombinant Narita or PR8 nuclear protein (11675-V08B; SinoBiological) after capturing with an anti-His antibody. .. After blocking with 1% blockAce (KAC, Kyoto, Japan), antibody titers were assessed by anti-mouse IgG1 Abs (1:10,000 dilution, Southern Biotech, Birmingham, AL, 1070-01 and 1:10,000 dilution, Bethyl, Montgomery, TX, A90-105P), anti-mouse IgG2b Abs (1:10,000 dilution, Bethyl, A90-109P), anti-mouse IgG2c Abs (1:10,000 dilution, AVIVA System Bio, San Diego, CA, OASB01582), and anti-mouse IgA Abs (1:10,000 dilution, Bethyl, A90-103A and A90-103P).

    Recombinant:

    Article Title: Influenza virus infection expands the breadth of antibody responses through IL-4 signalling in B cells
    Article Snippet: ELISA of LAH peptide and mini-HA, A/Narita/1/2009 HA 420-474 and recombinant HA2 (rHA2) were used as LAH and mini-HA antigens. .. IgG titers for the nuclear protein of influenza virus were measured by ELISA using His-conjugated recombinant Narita or PR8 nuclear protein (11675-V08B; SinoBiological) after capturing with an anti-His antibody. .. After blocking with 1% blockAce (KAC, Kyoto, Japan), antibody titers were assessed by anti-mouse IgG1 Abs (1:10,000 dilution, Southern Biotech, Birmingham, AL, 1070-01 and 1:10,000 dilution, Bethyl, Montgomery, TX, A90-105P), anti-mouse IgG2b Abs (1:10,000 dilution, Bethyl, A90-109P), anti-mouse IgG2c Abs (1:10,000 dilution, AVIVA System Bio, San Diego, CA, OASB01582), and anti-mouse IgA Abs (1:10,000 dilution, Bethyl, A90-103A and A90-103P).

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    Sino Biological influenza a virus hemagglutinin ha antibody rabbit mab
    Effect of λ-CGN on <t>influenza</t> A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice
    Influenza A Virus Hemagglutinin Ha Antibody Rabbit Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a virus hemagglutinin ha antibody rabbit mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a virus hemagglutinin ha antibody rabbit mab - by Bioz Stars, 2021-07
    94/100 stars
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    Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, In Vivo, Mouse Assay

    Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, Concentration Assay

    Antiviral effect of λ-CGN against influenza A virus in vivo. BALB/c mice (6–7 weeks old female) were mock-infected (black) or intranasally infected with maPR8 at 5 MLD 50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, purple) or a higher dose (5 mg/kg/d, green), followed by intranasal administration. Control mice received OSV-P orally twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight ( A ) and mortality ( B ) of mice were measured every day from days 0 to 14 post-infection. Data are expressed as the mean ± S.D. from five mice. Survival statistics were calculated by Log-rank (Mantel–Cox) test. ** P

    Journal: Scientific Reports

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

    doi: 10.1038/s41598-020-80896-9

    Figure Lengend Snippet: Antiviral effect of λ-CGN against influenza A virus in vivo. BALB/c mice (6–7 weeks old female) were mock-infected (black) or intranasally infected with maPR8 at 5 MLD 50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, purple) or a higher dose (5 mg/kg/d, green), followed by intranasal administration. Control mice received OSV-P orally twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight ( A ) and mortality ( B ) of mice were measured every day from days 0 to 14 post-infection. Data are expressed as the mean ± S.D. from five mice. Survival statistics were calculated by Log-rank (Mantel–Cox) test. ** P

    Article Snippet: Influenza viral NP and HA proteins were detected using mouse anti-NP (Cat. No., 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (Cat. No., 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: In Vivo, Mouse Assay, Infection

    Effect of λ-CGN on the influenza A virus entry. ( A ) HA inhibition assay. Two-fold serially diluted PR8 (from 2 3 to 2 11 ) in PBS was incubated with an equal volume of PBS or twofold increasing concentrations of λ-CGN for 20 min. HA titer in each combination was determined at 30 min after addition of 0.5% chicken RBC. HA titers are marked on the right side of the panel. ( B , C ) Confocal microscopy. MDCK cells were infected with PR8 (MOI, 5) in the absence (Mock) or presence of either λ-CGN or p-KG03 at a concentration of 10 μg/ml. At 4 h post-infection in the absence of CHX ( B ) or at 2.5 h in the presence of 10 μg/ml CHX ( C ), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×. The images were analyzed using ZEN blue software 3.1 ( www.zeiss.com ).

    Journal: Scientific Reports

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

    doi: 10.1038/s41598-020-80896-9

    Figure Lengend Snippet: Effect of λ-CGN on the influenza A virus entry. ( A ) HA inhibition assay. Two-fold serially diluted PR8 (from 2 3 to 2 11 ) in PBS was incubated with an equal volume of PBS or twofold increasing concentrations of λ-CGN for 20 min. HA titer in each combination was determined at 30 min after addition of 0.5% chicken RBC. HA titers are marked on the right side of the panel. ( B , C ) Confocal microscopy. MDCK cells were infected with PR8 (MOI, 5) in the absence (Mock) or presence of either λ-CGN or p-KG03 at a concentration of 10 μg/ml. At 4 h post-infection in the absence of CHX ( B ) or at 2.5 h in the presence of 10 μg/ml CHX ( C ), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×. The images were analyzed using ZEN blue software 3.1 ( www.zeiss.com ).

    Article Snippet: Influenza viral NP and HA proteins were detected using mouse anti-NP (Cat. No., 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (Cat. No., 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Inhibition, Incubation, Confocal Microscopy, Infection, Concentration Assay, Software

    Inhibition of infection by influenza A virus (H1N1) HA/NA- or SARS-CoV-2 spike-pseudotyped viruses. Lentiviral pseudotypes bearing influenza A virus (H1N1) HA and NA proteins (black bars) or SARS-CoV-2 spike protein (gray bars) were prepared, in which a firefly luciferase-expressing plasmid was incorporated. They were preincubated with mock or increasing concentrations of λ-CGN for 2 h at 37 °C and then infected into Vero E6 cells. On day 2, relative firefly luciferase activity (RLU) was determined by fixing the mock-treated sample at 100%. Data are expressed as the mean ± S.D. of three independent experiments. **** P

    Journal: Scientific Reports

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

    doi: 10.1038/s41598-020-80896-9

    Figure Lengend Snippet: Inhibition of infection by influenza A virus (H1N1) HA/NA- or SARS-CoV-2 spike-pseudotyped viruses. Lentiviral pseudotypes bearing influenza A virus (H1N1) HA and NA proteins (black bars) or SARS-CoV-2 spike protein (gray bars) were prepared, in which a firefly luciferase-expressing plasmid was incorporated. They were preincubated with mock or increasing concentrations of λ-CGN for 2 h at 37 °C and then infected into Vero E6 cells. On day 2, relative firefly luciferase activity (RLU) was determined by fixing the mock-treated sample at 100%. Data are expressed as the mean ± S.D. of three independent experiments. **** P

    Article Snippet: Influenza viral NP and HA proteins were detected using mouse anti-NP (Cat. No., 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (Cat. No., 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Inhibition, Infection, Luciferase, Expressing, Plasmid Preparation, Activity Assay

    Membrane fusion of cells infected with PR8. (A) Vero E6 cells were infected with PR8 at an MOI of 0.5 at 37°C. At 16 h p.i., cells were preincubated with TPCK-trypsin (5 μg/ml) together with DMSO, 2 μM salinomycin, or 0.5 μg/ml anti-HA2 antibody. Cellular membrane fusion was initiated by exposing samples to the indicated conditions (pH 7.0 or 5.6). After staining with Giemsa, fixed cells were visualized by microscopy. Original magnification, ×200. (B) The relative number of nuclei in syncytia was counted from 16 representative images per sample at pH 5.6. Statistical significance was analyzed by comparing differences between the DMSO-treated group and the compound- or antibody-treated groups. ****, P

    Journal: Journal of Virology

    Article Title: Salinomycin Inhibits Influenza Virus Infection by Disrupting Endosomal Acidification and Viral Matrix Protein 2 Function

    doi: 10.1128/JVI.01441-18

    Figure Lengend Snippet: Membrane fusion of cells infected with PR8. (A) Vero E6 cells were infected with PR8 at an MOI of 0.5 at 37°C. At 16 h p.i., cells were preincubated with TPCK-trypsin (5 μg/ml) together with DMSO, 2 μM salinomycin, or 0.5 μg/ml anti-HA2 antibody. Cellular membrane fusion was initiated by exposing samples to the indicated conditions (pH 7.0 or 5.6). After staining with Giemsa, fixed cells were visualized by microscopy. Original magnification, ×200. (B) The relative number of nuclei in syncytia was counted from 16 representative images per sample at pH 5.6. Statistical significance was analyzed by comparing differences between the DMSO-treated group and the compound- or antibody-treated groups. ****, P

    Article Snippet: Viral NP, HA, M1, and M2 proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China), rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological), mouse anti-M1 (catalog no. sc-57881; Santa Cruz Biotechnology, Santa Cruz, CA), and mouse anti-M2 (catalog no. sc-32238; Santa Cruz Biotechnology) antibodies, respectively.

    Techniques: Infection, Staining, Microscopy

    In vivo antiviral activity of nylidrin against a mouse-adapted influenza A virus (H1N1), maPR8. Before viral challenge, maPR8 (5 MLD 50 ) was preincubated with DMSO (virus only) or with nylidrin (10 mg/kg) at room temperature for 30 min. Mice were mock-infected or infected with the preincubated samples. As a positive control, OSV-P (10 mg/kg/day) was orally administered twice a day beginning 4 h before virus infection at 8-h intervals from days 0 to 5 post-infection. Body weight ( A ) and mortality ( B ) were measured every day for 15 days. Statistical analysis was performed using a two-tailed unpaired t -test relative to the virus-only group. n = 5; **, p

    Journal: Viruses

    Article Title: In Vitro and In Vivo Antiviral Activity of Nylidrin by Targeting the Hemagglutinin 2-Mediated Membrane Fusion of Influenza A Virus

    doi: 10.3390/v12050581

    Figure Lengend Snippet: In vivo antiviral activity of nylidrin against a mouse-adapted influenza A virus (H1N1), maPR8. Before viral challenge, maPR8 (5 MLD 50 ) was preincubated with DMSO (virus only) or with nylidrin (10 mg/kg) at room temperature for 30 min. Mice were mock-infected or infected with the preincubated samples. As a positive control, OSV-P (10 mg/kg/day) was orally administered twice a day beginning 4 h before virus infection at 8-h intervals from days 0 to 5 post-infection. Body weight ( A ) and mortality ( B ) were measured every day for 15 days. Statistical analysis was performed using a two-tailed unpaired t -test relative to the virus-only group. n = 5; **, p

    Article Snippet: Viral proteins NP, HA, and M1 were detected by immunoblotting the appropriate antibodies: mouse anti-NP (cat no. 11675-T62; Sino Biological, Beijing, China), rabbit anti-HA2 (cat no. 86001-RM01; Sino Biological), and mouse anti-M1 (cat no. sc-57881; Santa Cruz Biotechnology, Dallas, TX, USA), respectively.

    Techniques: In Vivo, Activity Assay, Mouse Assay, Infection, Positive Control, Two Tailed Test