ha rabbit igg  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Influenza B Hemagglutinin HA Antibody Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza B Hemagglutinin Catalog 11053 V08H ACA33493 1 Met 1 Ala 555
    Catalog Number:
    11053-R004
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    Influenza B
    Applications:
    WB,ELISA
    Immunogen:
    Recombinant Influenza B virus HA protein (Catalog#11053-V08H)
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Sino Biological ha rabbit igg
    Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A <t>H5N1</t> (A/Vietnam/1194/2004) HA <t>IgG,</t> followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza B Hemagglutinin Catalog 11053 V08H ACA33493 1 Met 1 Ala 555
    https://www.bioz.com/result/ha rabbit igg/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ha rabbit igg - by Bioz Stars, 2021-10
    94/100 stars

    Images

    1) Product Images from "Novel Mutations Evading Avian Immunity around the Receptor Binding Site of the Clade 2.3.2.1c Hemagglutinin Gene Reduce Viral Thermostability and Mammalian Pathogenicity"

    Article Title: Novel Mutations Evading Avian Immunity around the Receptor Binding Site of the Clade 2.3.2.1c Hemagglutinin Gene Reduce Viral Thermostability and Mammalian Pathogenicity

    Journal: Viruses

    doi: 10.3390/v11100923

    Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A H5N1 (A/Vietnam/1194/2004) HA IgG, followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).
    Figure Legend Snippet: Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A H5N1 (A/Vietnam/1194/2004) HA IgG, followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).

    Techniques Used: Western Blot, Recombinant, SDS Page, Incubation, Molecular Weight

    Related Articles

    Expressing:

    Article Title: Combined DLL3-targeted bispecific antibody with PD-1 inhibition is efficient to suppress small cell lung cancer growth
    Article Snippet: .. For PD-L1 expression analysis, a rabbit monoclonal antibody (Sinobiological, Beijing, China) was incubated with the SCLC cells and the antibody binding was detected by allophycocyanin (APC)-conjugated goat-anti-rabbit IgG (Jackson ImmunoResearch, West Grove, Pennsylvania). ..

    Incubation:

    Article Title: Combined DLL3-targeted bispecific antibody with PD-1 inhibition is efficient to suppress small cell lung cancer growth
    Article Snippet: .. For PD-L1 expression analysis, a rabbit monoclonal antibody (Sinobiological, Beijing, China) was incubated with the SCLC cells and the antibody binding was detected by allophycocyanin (APC)-conjugated goat-anti-rabbit IgG (Jackson ImmunoResearch, West Grove, Pennsylvania). ..

    Article Title: Boron doped diamond thin films for the electrochemical detection of SARS-CoV-2 S1 protein
    Article Snippet: .. For the antibody attachment, the samples were incubated overnight at 4 °C in 400 μL of 4 μg/mL biotinylated SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit Mab (Sino Biological) in PBS. ..

    Article Title: Novel Mutations Evading Avian Immunity around the Receptor Binding Site of the Clade 2.3.2.1c Hemagglutinin Gene Reduce Viral Thermostability and Mammalian Pathogenicity
    Article Snippet: .. The proteins were transferred to a nitrocellulose membrane (Life Technologies Co.), and the membrane was incubated with anti-H5N1 virus (A/Vietnam/1194/2004), HA rabbit IgG (Sino Biological Inc., Beijing, China), followed by incubation with horseradish peroxidase conjugated-goat anti-rabbit IgG (Bethyl Laboratories Inc., Montgomery, AL, USA). ..

    Binding Assay:

    Article Title: Combined DLL3-targeted bispecific antibody with PD-1 inhibition is efficient to suppress small cell lung cancer growth
    Article Snippet: .. For PD-L1 expression analysis, a rabbit monoclonal antibody (Sinobiological, Beijing, China) was incubated with the SCLC cells and the antibody binding was detected by allophycocyanin (APC)-conjugated goat-anti-rabbit IgG (Jackson ImmunoResearch, West Grove, Pennsylvania). ..

    Blocking Assay:

    Article Title: Characteristic and quantifiable COVID-19-like abnormalities in CT- and PET/CT-imaged lungs of SARS-CoV-2-infected crab-eating macaques (Macaca fascicularis)
    Article Snippet: .. The primary antibody was SARS-CoV-2 Nucleoprotein/NP Antibody, Rabbit mAb (Sino Biological, Chesterbrook, PA, USA) prepared at 1:8,000 in blocking buffer at room temperature. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Sino Biological ha rabbit igg
    Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A <t>H5N1</t> (A/Vietnam/1194/2004) HA <t>IgG,</t> followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).
    Ha Rabbit Igg, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ha rabbit igg/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ha rabbit igg - by Bioz Stars, 2021-10
    94/100 stars
      Buy from Supplier

    86
    Sino Biological rabbit igg anti ha
    Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A <t>H5N1</t> (A/Vietnam/1194/2004) HA <t>IgG,</t> followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).
    Rabbit Igg Anti Ha, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit igg anti ha/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit igg anti ha - by Bioz Stars, 2021-10
    86/100 stars
      Buy from Supplier

    Image Search Results


    Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A H5N1 (A/Vietnam/1194/2004) HA IgG, followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).

    Journal: Viruses

    Article Title: Novel Mutations Evading Avian Immunity around the Receptor Binding Site of the Clade 2.3.2.1c Hemagglutinin Gene Reduce Viral Thermostability and Mammalian Pathogenicity

    doi: 10.3390/v11100923

    Figure Lengend Snippet: Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A H5N1 (A/Vietnam/1194/2004) HA IgG, followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).

    Article Snippet: The proteins were transferred to a nitrocellulose membrane (Life Technologies Co.), and the membrane was incubated with anti-H5N1 virus (A/Vietnam/1194/2004), HA rabbit IgG (Sino Biological Inc., Beijing, China), followed by incubation with horseradish peroxidase conjugated-goat anti-rabbit IgG (Bethyl Laboratories Inc., Montgomery, AL, USA).

    Techniques: Western Blot, Recombinant, SDS Page, Incubation, Molecular Weight