ha protein  (Sino Biological)


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    Name:
    Influenza B Hemagglutinin HA Protein
    Description:
    A DNA sequence encoding the extracellular domain of Influenza B B Florida 4 2006 ACA33493 1 hemagglutinin Met 1 Ala 555 Native HA1 HA2 uncleaved was expressed with a C terminal polyhistidine tag
    Catalog Number:
    11053-V08H
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological ha protein
    A DNA sequence encoding the extracellular domain of Influenza B B Florida 4 2006 ACA33493 1 hemagglutinin Met 1 Ala 555 Native HA1 HA2 uncleaved was expressed with a C terminal polyhistidine tag
    https://www.bioz.com/result/ha protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ha protein - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Recombinant baculovirus vaccine expressing hemagglutinin of H7N9 avian influenza virus confers full protection against lethal highly pathogenic H7N9 virus infection in chickens.
    Article Snippet: The emergent highly pathogenic avian influenza A (H7N9) (HPAI) virus is a major public concern in China. .. The emergent highly pathogenic avian influenza A (H7N9) (HPAI) virus is a major public concern in China. ..

    Purification:

    Article Title: Recombinant baculovirus vaccine expressing hemagglutinin of H7N9 avian influenza virus confers full protection against lethal highly pathogenic H7N9 virus infection in chickens.
    Article Snippet: The emergent highly pathogenic avian influenza A (H7N9) (HPAI) virus is a major public concern in China. .. The emergent highly pathogenic avian influenza A (H7N9) (HPAI) virus is a major public concern in China. ..

    Staining:

    Article Title: Vaccination against Influenza with Recombinant Hemagglutinin Expressed by Schizochytrium sp. Confers Protective Immunity
    Article Snippet: CFS or other protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on a NuPAGE® Novex® 12% bis-tris gel (Invitrogen, Carlsbad, CA) under reducing conditions with 3-(N-morpholino) propanesulfonic acid - sodium dodecylsulfate (MOPS-SDS) running buffer, unless indicated otherwise in the text. .. The proteins were then stained with SimplyBlue Safe Stain (Invitrogen, Carlsbad, CA, USA) or transferred onto polyvinylidene fluoride membrane and probed for the presence of HA protein with anti-PR8 virus antiserum from rabbit, catalog number 11684-RP01 (Sino Biological Inc., Beijing, China), followed by anti-rabbit IgG (Fc) secondary antibody coupled to alkaline phosphatase (Promega Corporation, Madison, WI). .. The proteins were then stained with SimplyBlue Safe Stain (Invitrogen, Carlsbad, CA, USA) or transferred onto polyvinylidene fluoride membrane and probed for the presence of HA protein with anti-PR8 virus antiserum from rabbit, catalog number 11684-RP01 (Sino Biological Inc., Beijing, China), followed by anti-rabbit IgG (Fc) secondary antibody coupled to alkaline phosphatase (Promega Corporation, Madison, WI).

    Recombinant:

    Article Title: Safety and immunogenicity of a replication-deficient H5N1 influenza virus vaccine lacking NS1.
    Article Snippet: Traditional inactivated influenza vaccines are the type of vaccines that were most frequently developed for immunization against the highly pathogenic avian H5N1 influenza virus. .. Traditional inactivated influenza vaccines are the type of vaccines that were most frequently developed for immunization against the highly pathogenic avian H5N1 influenza virus. .. Traditional inactivated influenza vaccines are the type of vaccines that were most frequently developed for immunization against the highly pathogenic avian H5N1 influenza virus.

    Article Title: Porous Nanoparticles With Self-Adjuvanting M2e-Fusion Protein and Recombinant Hemagglutinin Provide Strong and Broadly Protective Immunity Against Influenza Virus Infections
    Article Snippet: CTA1-3M2e-DD and HA loading into nanoparticlesThe fusion proteins or trimeric HA were loaded into premade NPL at a mass ratio 1:5 (protein:NPL), by mixing the proteins with NPLs followed by incubation for 30 min at room temperature. .. The recombinant extracellular domain (Met 1-Gln 528) of the hemagglutinin (HA1+HA2) was derived from Influenza A Virus H1N1 (A/Puerto Rico/8/34 virus strain) fused with a C-terminal polyhistidine tag (Sino Biological Inc., China) was resuspended in 1.98% Empigen® BB (N,N- Dimethyl-N-dodecylglycine betaine, Sigma-Aldrich, France) obtaining a protein concentration of 1 mg/ml. .. Then HA was incubated with either NPL or CTA1-3M2e-DD:NPL at r.t. to obtain a formulation with a mass ratio 1:5 (protein:NPL).

    Derivative Assay:

    Article Title: Porous Nanoparticles With Self-Adjuvanting M2e-Fusion Protein and Recombinant Hemagglutinin Provide Strong and Broadly Protective Immunity Against Influenza Virus Infections
    Article Snippet: CTA1-3M2e-DD and HA loading into nanoparticlesThe fusion proteins or trimeric HA were loaded into premade NPL at a mass ratio 1:5 (protein:NPL), by mixing the proteins with NPLs followed by incubation for 30 min at room temperature. .. The recombinant extracellular domain (Met 1-Gln 528) of the hemagglutinin (HA1+HA2) was derived from Influenza A Virus H1N1 (A/Puerto Rico/8/34 virus strain) fused with a C-terminal polyhistidine tag (Sino Biological Inc., China) was resuspended in 1.98% Empigen® BB (N,N- Dimethyl-N-dodecylglycine betaine, Sigma-Aldrich, France) obtaining a protein concentration of 1 mg/ml. .. Then HA was incubated with either NPL or CTA1-3M2e-DD:NPL at r.t. to obtain a formulation with a mass ratio 1:5 (protein:NPL).

    Protein Concentration:

    Article Title: Porous Nanoparticles With Self-Adjuvanting M2e-Fusion Protein and Recombinant Hemagglutinin Provide Strong and Broadly Protective Immunity Against Influenza Virus Infections
    Article Snippet: CTA1-3M2e-DD and HA loading into nanoparticlesThe fusion proteins or trimeric HA were loaded into premade NPL at a mass ratio 1:5 (protein:NPL), by mixing the proteins with NPLs followed by incubation for 30 min at room temperature. .. The recombinant extracellular domain (Met 1-Gln 528) of the hemagglutinin (HA1+HA2) was derived from Influenza A Virus H1N1 (A/Puerto Rico/8/34 virus strain) fused with a C-terminal polyhistidine tag (Sino Biological Inc., China) was resuspended in 1.98% Empigen® BB (N,N- Dimethyl-N-dodecylglycine betaine, Sigma-Aldrich, France) obtaining a protein concentration of 1 mg/ml. .. Then HA was incubated with either NPL or CTA1-3M2e-DD:NPL at r.t. to obtain a formulation with a mass ratio 1:5 (protein:NPL).

    other:

    Article Title: Hemagglutinin Quantitative ELISA-based Potency Assay for Trivalent Seasonal Influenza Vaccine Using Group-Specific Universal Monoclonal Antibodies
    Article Snippet: Likewise, the 10F8 clone, universally bound to the HAs of IBV of both Yamagata (B/Phuket/3073/2013 and B/Massachusetts/03/2010) and Victoria lineages (B/Brisbane/60/2018 and B/Maryland/15/2016) but failed to bind to HAs of IAVs (Fig. ).

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  • 94
    Sino Biological pr8
    Heterosubtypic protection against H1 virus by anti–MHCII-MIX vaccination is independent of T cells. ( A and B ) BALB/c mice ( n = 7 per group) were immunized three times (weeks 0, 4, and 7) with 25 μg of DNA/EP of the indicated vaccines. Sera from mice of each group were harvested 2 wk after the third vaccination and transferred i.v. to naive mice ( n = 10 per group) before a lethal challenge with 5 × LD 50 <t>PR8.</t> Mice were monitored for weight loss (A) and survival (B). The results are representative of two independent experiments. ( C and D ) BALB/c mice ( n = 10) were immunized three times (weeks 0, 4, and 7) with 25 μg of DNA/EP of the indicated vaccines and subsequently were injected every other day from day 12 after the third vaccination with depleting mAbs against CD4 and CD8 T cells or isotype-matched control mAbs. A 5 × LD 50 lethal dose of PR8 was administered at day 14, and the mice were monitored for weight loss (C) and survival (D). Efficient depletion was confirmed by staining of splenocytes. * p
    Pr8, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pr8/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pr8 - by Bioz Stars, 2021-06
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    80
    Sino Biological recombinant mex4108 ha protein plasma
    Validated miRNA targets are involved in immune response and cell proliferation in the lungs. Expression levels of (A) KRAS , BLIMP1 , and CDK6 (targets of let-7f); (B) SIRT1 , ZAP70 , and MYC (targets of miR-34c); (C) CAMTA1 (target of miR-129); and (D) PTEN (target of miR-18b) in BAL from rhesus macaques (five aged and three young adults) following <t>MEX4108</t> infection are shown. Expression of mRNA was normalized to expression of RPL32 . Changes in gene expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. (A–D) *let-7f, miR-34c, miR-129, miR-18-b, KRAS , SIRT1 , CAMTA1 , and PTEN ; ‡ KRAS and ZAP70 ; † CDK6 and MYC . * p
    Recombinant Mex4108 Ha Protein Plasma, supplied by Sino Biological, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mex4108 ha protein plasma/product/Sino Biological
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant mex4108 ha protein plasma - by Bioz Stars, 2021-06
    80/100 stars
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    94
    Sino Biological recombinant ha protein influenza a virus h1n1
    Antiviral activity of ginseng extract (GE) against 2009 pandemic <t>H1N1</t> <t>influenza</t> A virus infection. (A) A dose dependent reduction in viral titer was observed when MDCK cells were infected with GE-pretreated A/Nanchang/8002/2009 (H1N1) virus. Protective effect of GE was observed on (B) weight loss and (C) survival of mice infected with A/Nanchang/8002/2009 (H1N1) virus. GE oral_ mice treated with 80mg/kg of GE by oral route. GE_ mice were infected with a mixture of 10 3 EID 50 of virus and GE. Mock_ there was no GE treatment given. *** P
    Recombinant Ha Protein Influenza A Virus H1n1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant ha protein influenza a virus h1n1/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant ha protein influenza a virus h1n1 - by Bioz Stars, 2021-06
    94/100 stars
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    N/A
    A DNA sequence encoding the Influenza A virus A duck Hong Kong 562 1979 H10N9 hemagglutinin ABI84469 1 Met1 Asp524 termed as HA was expressed with a polyhistidine tag at
      Buy from Supplier

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    Heterosubtypic protection against H1 virus by anti–MHCII-MIX vaccination is independent of T cells. ( A and B ) BALB/c mice ( n = 7 per group) were immunized three times (weeks 0, 4, and 7) with 25 μg of DNA/EP of the indicated vaccines. Sera from mice of each group were harvested 2 wk after the third vaccination and transferred i.v. to naive mice ( n = 10 per group) before a lethal challenge with 5 × LD 50 PR8. Mice were monitored for weight loss (A) and survival (B). The results are representative of two independent experiments. ( C and D ) BALB/c mice ( n = 10) were immunized three times (weeks 0, 4, and 7) with 25 μg of DNA/EP of the indicated vaccines and subsequently were injected every other day from day 12 after the third vaccination with depleting mAbs against CD4 and CD8 T cells or isotype-matched control mAbs. A 5 × LD 50 lethal dose of PR8 was administered at day 14, and the mice were monitored for weight loss (C) and survival (D). Efficient depletion was confirmed by staining of splenocytes. * p

    Journal: The Journal of Immunology Author Choice

    Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza

    doi: 10.4049/jimmunol.1701088

    Figure Lengend Snippet: Heterosubtypic protection against H1 virus by anti–MHCII-MIX vaccination is independent of T cells. ( A and B ) BALB/c mice ( n = 7 per group) were immunized three times (weeks 0, 4, and 7) with 25 μg of DNA/EP of the indicated vaccines. Sera from mice of each group were harvested 2 wk after the third vaccination and transferred i.v. to naive mice ( n = 10 per group) before a lethal challenge with 5 × LD 50 PR8. Mice were monitored for weight loss (A) and survival (B). The results are representative of two independent experiments. ( C and D ) BALB/c mice ( n = 10) were immunized three times (weeks 0, 4, and 7) with 25 μg of DNA/EP of the indicated vaccines and subsequently were injected every other day from day 12 after the third vaccination with depleting mAbs against CD4 and CD8 T cells or isotype-matched control mAbs. A 5 × LD 50 lethal dose of PR8 was administered at day 14, and the mice were monitored for weight loss (C) and survival (D). Efficient depletion was confirmed by staining of splenocytes. * p

    Article Snippet: ELISA 96-well plates (Costar 3590) were coated with one of the following recombinant influenza HA proteins: PR8 (11684-V08H; Sino Biological), A/Hong Kong/483/97 (H5N1) (11689-V08H; Sino Biological), A/northern shoveler/California/HKWF115/07 (H6N1) (MBS434125; MyBioSource, San Diego, CA, or 11723-V08H; Sino Biological), A/pintail duck/Alberta/114/1979 (H8N4) (11722-V08H; Sino Biological), A/Hong Kong/1073/99 (H9N2) (11229-V08H; Sino Biological), A/duck/Yangzhou/906/2002 (H11N2) (11705-V08H; Sino Biological), A/black-headed gull/Netherlands/1/00 (H13N8) (11721-V08H; Sino Biological), A/Shanghai/1/2013 (H7N9) (40104-V08B; Sino Biological), or A/Hong Kong/1/1968 (H3N2) (40116-V08B; Sino Biological).

    Techniques: Mouse Assay, Injection, Staining

    Vaccination with anti–MHCII-MIX confers heterosubtypic protection against H1 influenza viruses not included in the vaccine mixture. ( A – F ) BALB/c mice ( n = 6 per group) were immunized three times (weeks 0, 4, and 12, arrows) with 25 μg of DNA/EP of the indicated vaccines. As positive controls, anti–MHCII-H1(PR8) and anti–MHCII-H1(Cal07) were included. Serum IgG Abs were measured against PR8 (A) or Cal07 (B) in ELISAs (mean ± SEM). Microneutralization assays were performed on sera harvested 2 wk after the third vaccination with PR8 (C) and Cal07 (D). The dashed line indicates 50% threshold for positive neutralization. Two weeks after the third vaccination (above), mice were challenged with a lethal dose of Cal07 and monitored for weight loss (± SEM) (E) and survival (F). ( G and H ) In a new experiment, mice were vaccinated at weeks 0, 4, and 12 (as above) and challenged with a lethal dose of influenza PR8 2 wk after the third vaccination ( n = 10 per group [black], n = 6 per group [gray]). Mice were monitored for weight loss (± SEM) (G) and survival (H). * p

    Journal: The Journal of Immunology Author Choice

    Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza

    doi: 10.4049/jimmunol.1701088

    Figure Lengend Snippet: Vaccination with anti–MHCII-MIX confers heterosubtypic protection against H1 influenza viruses not included in the vaccine mixture. ( A – F ) BALB/c mice ( n = 6 per group) were immunized three times (weeks 0, 4, and 12, arrows) with 25 μg of DNA/EP of the indicated vaccines. As positive controls, anti–MHCII-H1(PR8) and anti–MHCII-H1(Cal07) were included. Serum IgG Abs were measured against PR8 (A) or Cal07 (B) in ELISAs (mean ± SEM). Microneutralization assays were performed on sera harvested 2 wk after the third vaccination with PR8 (C) and Cal07 (D). The dashed line indicates 50% threshold for positive neutralization. Two weeks after the third vaccination (above), mice were challenged with a lethal dose of Cal07 and monitored for weight loss (± SEM) (E) and survival (F). ( G and H ) In a new experiment, mice were vaccinated at weeks 0, 4, and 12 (as above) and challenged with a lethal dose of influenza PR8 2 wk after the third vaccination ( n = 10 per group [black], n = 6 per group [gray]). Mice were monitored for weight loss (± SEM) (G) and survival (H). * p

    Article Snippet: ELISA 96-well plates (Costar 3590) were coated with one of the following recombinant influenza HA proteins: PR8 (11684-V08H; Sino Biological), A/Hong Kong/483/97 (H5N1) (11689-V08H; Sino Biological), A/northern shoveler/California/HKWF115/07 (H6N1) (MBS434125; MyBioSource, San Diego, CA, or 11723-V08H; Sino Biological), A/pintail duck/Alberta/114/1979 (H8N4) (11722-V08H; Sino Biological), A/Hong Kong/1073/99 (H9N2) (11229-V08H; Sino Biological), A/duck/Yangzhou/906/2002 (H11N2) (11705-V08H; Sino Biological), A/black-headed gull/Netherlands/1/00 (H13N8) (11721-V08H; Sino Biological), A/Shanghai/1/2013 (H7N9) (40104-V08B; Sino Biological), or A/Hong Kong/1/1968 (H3N2) (40116-V08B; Sino Biological).

    Techniques: Mouse Assay, Neutralization

    Characterization of vaccine proteins. ( A ) The MHCII-targeted vaccine proteins consist of a targeting unit (scFv) that is specific for MHC class II (I), a dimerization unit containing a shortened hinge and C H 3 from human γ3 (II), and an antigenic unit encoding one of the six indicated HAs (III). Nontargeted controls have been prepared by replacement of the targeting unit with an scFv that is specific for the hapten NIP. ( B ) Supernatants from HEK 293E cells transfected with the DNA plasmids encoding the indicated vaccines were analyzed in sandwich ELISAs. An MHCII-targeted vaccine encoding HA from PR8 (anti–MHCII-H1) was included as positive control.

    Journal: The Journal of Immunology Author Choice

    Article Title: Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza

    doi: 10.4049/jimmunol.1701088

    Figure Lengend Snippet: Characterization of vaccine proteins. ( A ) The MHCII-targeted vaccine proteins consist of a targeting unit (scFv) that is specific for MHC class II (I), a dimerization unit containing a shortened hinge and C H 3 from human γ3 (II), and an antigenic unit encoding one of the six indicated HAs (III). Nontargeted controls have been prepared by replacement of the targeting unit with an scFv that is specific for the hapten NIP. ( B ) Supernatants from HEK 293E cells transfected with the DNA plasmids encoding the indicated vaccines were analyzed in sandwich ELISAs. An MHCII-targeted vaccine encoding HA from PR8 (anti–MHCII-H1) was included as positive control.

    Article Snippet: ELISA 96-well plates (Costar 3590) were coated with one of the following recombinant influenza HA proteins: PR8 (11684-V08H; Sino Biological), A/Hong Kong/483/97 (H5N1) (11689-V08H; Sino Biological), A/northern shoveler/California/HKWF115/07 (H6N1) (MBS434125; MyBioSource, San Diego, CA, or 11723-V08H; Sino Biological), A/pintail duck/Alberta/114/1979 (H8N4) (11722-V08H; Sino Biological), A/Hong Kong/1073/99 (H9N2) (11229-V08H; Sino Biological), A/duck/Yangzhou/906/2002 (H11N2) (11705-V08H; Sino Biological), A/black-headed gull/Netherlands/1/00 (H13N8) (11721-V08H; Sino Biological), A/Shanghai/1/2013 (H7N9) (40104-V08B; Sino Biological), or A/Hong Kong/1/1968 (H3N2) (40116-V08B; Sino Biological).

    Techniques: Transfection, Positive Control

    Validated miRNA targets are involved in immune response and cell proliferation in the lungs. Expression levels of (A) KRAS , BLIMP1 , and CDK6 (targets of let-7f); (B) SIRT1 , ZAP70 , and MYC (targets of miR-34c); (C) CAMTA1 (target of miR-129); and (D) PTEN (target of miR-18b) in BAL from rhesus macaques (five aged and three young adults) following MEX4108 infection are shown. Expression of mRNA was normalized to expression of RPL32 . Changes in gene expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. (A–D) *let-7f, miR-34c, miR-129, miR-18-b, KRAS , SIRT1 , CAMTA1 , and PTEN ; ‡ KRAS and ZAP70 ; † CDK6 and MYC . * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Validated miRNA targets are involved in immune response and cell proliferation in the lungs. Expression levels of (A) KRAS , BLIMP1 , and CDK6 (targets of let-7f); (B) SIRT1 , ZAP70 , and MYC (targets of miR-34c); (C) CAMTA1 (target of miR-129); and (D) PTEN (target of miR-18b) in BAL from rhesus macaques (five aged and three young adults) following MEX4108 infection are shown. Expression of mRNA was normalized to expression of RPL32 . Changes in gene expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. (A–D) *let-7f, miR-34c, miR-129, miR-18-b, KRAS , SIRT1 , CAMTA1 , and PTEN ; ‡ KRAS and ZAP70 ; † CDK6 and MYC . * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Expressing, Infection

    Differentially expressed miRNAs in PBMCs following MEX4108. Expression levels of miR-18b (A) , miR-20a (B) , miR-192 (C) , miR-451 (D) , miR-138 (E) , miR-193b (F) , miR-132 (G) , let-7f (H) , and miR-146b (I) in PBMCs from rhesus macaques ( n = 11; five aged and six young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Differentially expressed miRNAs in PBMCs following MEX4108. Expression levels of miR-18b (A) , miR-20a (B) , miR-192 (C) , miR-451 (D) , miR-138 (E) , miR-193b (F) , miR-132 (G) , let-7f (H) , and miR-146b (I) in PBMCs from rhesus macaques ( n = 11; five aged and six young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Expressing, Infection, Quantitative RT-PCR

    MEX4108 infection results in increased frequency of pDCs and robust production of cytokine, chemokine, and growth factor levels in the lungs. (A) The frequencies of DCs (lin − CD14 − HLA-DR + ) and macrophages/monocytes (lin − CD14 + HLA-DR − ) in BAL cells were measured by flow cytometry. (B) The frequencies of mDC (CD123 − CD11c + ) and pDC (CD123 + CD11c − ) in BAL cells were measured by flow cytometry. Longitudinal analyses of the frequency of immune cells within BAL were carried out using a one-way repeated-measures ANOVA model, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values. Mean ± SEM are shown. * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: MEX4108 infection results in increased frequency of pDCs and robust production of cytokine, chemokine, and growth factor levels in the lungs. (A) The frequencies of DCs (lin − CD14 − HLA-DR + ) and macrophages/monocytes (lin − CD14 + HLA-DR − ) in BAL cells were measured by flow cytometry. (B) The frequencies of mDC (CD123 − CD11c + ) and pDC (CD123 + CD11c − ) in BAL cells were measured by flow cytometry. Longitudinal analyses of the frequency of immune cells within BAL were carried out using a one-way repeated-measures ANOVA model, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values. Mean ± SEM are shown. * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Infection, Flow Cytometry, Cytometry

    Model of differentially expressed microRNAs, gene targets, and immune response in the lungs following MEX4108 infection.

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Model of differentially expressed microRNAs, gene targets, and immune response in the lungs following MEX4108 infection.

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Infection

    Pandemic H1N1 virus replicates to similar levels in young and aged macaques. Viral loads were measured using qRT-PCR using primers and probes specific for MEX4108 hemagglutinin (HA) in throat swabs (A) , nasal swabs (B) , ocular swabs (C) , and BAL fluid (D) . Viral genome copy number data were log transformed with base 10 and longitudinal changes of viral genome copy number between aged and young adults were compared using a two-way ANOVA, followed by Bonferroni's multiple comparison post-test to determine differences in viral load. Longitudinal changes were compared using repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values, mean ± SEM are shown. (A–D) * for aged animals; ‡ for young adult animals; *** ,‡‡ p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Pandemic H1N1 virus replicates to similar levels in young and aged macaques. Viral loads were measured using qRT-PCR using primers and probes specific for MEX4108 hemagglutinin (HA) in throat swabs (A) , nasal swabs (B) , ocular swabs (C) , and BAL fluid (D) . Viral genome copy number data were log transformed with base 10 and longitudinal changes of viral genome copy number between aged and young adults were compared using a two-way ANOVA, followed by Bonferroni's multiple comparison post-test to determine differences in viral load. Longitudinal changes were compared using repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test to explore differences between days postinfection and baseline (day 0) values, mean ± SEM are shown. (A–D) * for aged animals; ‡ for young adult animals; *** ,‡‡ p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Quantitative RT-PCR, Transformation Assay

    Differentially expressed miRNAs in BAL cells following MEX4108. Expression levels of let-7f (A) , miR-34c (B) , miR-129 (C) , miR-18b (D) , miR-146b (E) , miR-132 (F) , miR-192 (G) , and miR-138 (H) in BAL cells from rhesus macaques ( n = 8; five aged and three young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Journal: Viral Immunology

    Article Title: microRNAs Regulate Host Immune Response and Pathogenesis During Influenza Infection in Rhesus Macaques

    doi: 10.1089/vim.2015.0074

    Figure Lengend Snippet: Differentially expressed miRNAs in BAL cells following MEX4108. Expression levels of let-7f (A) , miR-34c (B) , miR-129 (C) , miR-18b (D) , miR-146b (E) , miR-132 (F) , miR-192 (G) , and miR-138 (H) in BAL cells from rhesus macaques ( n = 8; five aged and three young adults) following MEX4108 infection were determined using qRT-PCR. Expression of miRNAs was normalized to expression of U6 snRNA. Changes in microRNA expression postinfection were determined using one-way repeated-measures ANOVA, followed by Dunnett's multiple comparison post-test. Mean ± SEM are shown. * p

    Article Snippet: IgG and IgA binding antibody titers were measured in plasma and BAL supernatant by enzyme-linked immunosorbent assay (ELISA) using plates coated with 1 μg/mL recombinant MEX4108 HA protein (plasma) overnight at 4°C (Sino Biological, Inc., Beijing, China).

    Techniques: Expressing, Infection, Quantitative RT-PCR

    Antiviral activity of ginseng extract (GE) against 2009 pandemic H1N1 influenza A virus infection. (A) A dose dependent reduction in viral titer was observed when MDCK cells were infected with GE-pretreated A/Nanchang/8002/2009 (H1N1) virus. Protective effect of GE was observed on (B) weight loss and (C) survival of mice infected with A/Nanchang/8002/2009 (H1N1) virus. GE oral_ mice treated with 80mg/kg of GE by oral route. GE_ mice were infected with a mixture of 10 3 EID 50 of virus and GE. Mock_ there was no GE treatment given. *** P

    Journal: PLoS ONE

    Article Title: Inhibition of influenza A virus infection by ginsenosides

    doi: 10.1371/journal.pone.0171936

    Figure Lengend Snippet: Antiviral activity of ginseng extract (GE) against 2009 pandemic H1N1 influenza A virus infection. (A) A dose dependent reduction in viral titer was observed when MDCK cells were infected with GE-pretreated A/Nanchang/8002/2009 (H1N1) virus. Protective effect of GE was observed on (B) weight loss and (C) survival of mice infected with A/Nanchang/8002/2009 (H1N1) virus. GE oral_ mice treated with 80mg/kg of GE by oral route. GE_ mice were infected with a mixture of 10 3 EID 50 of virus and GE. Mock_ there was no GE treatment given. *** P

    Article Snippet: Immunoblot assayDifferent concentrations of ginsenosides Rb1 were mixed with recombinant HA protein -Influenza A virus H1N1 (A/California/07/2009) (Sino Biological Inc., Beijing, China) and incubated at 37°C for 1 hour and then was spotted onto PVDF membrane.

    Techniques: Activity Assay, Infection, Mouse Assay