Structured Review

Millipore anti h3k56ac
A Light microscopy image (1 mm scale bar) of isolated C. glandular trichomes. B Spearman correlation matrix of RNA-seq and ChIP-seq data for H3K4me3, <t>H3K56ac,</t> H3K27me3, and H2A.Z in glandular trichomes. C C. sativa karyotype with glandular trichome data density plots (i) all genes (ii) transcribed genes (iii) H3K4me3 peaks (iv) H3K56ac peaks (v) untranscribed genes (vi) H3K27me3 domains and (vii) H2A.Z domains. D H2A.Z, H3K27me3, H3K56ac, and H3K4me3 peak annotation. E Scale-region gene plots and associated heatmaps of glandular trichome H3K4me3, H3K56ac, H3K27me3, and H2A.Z reads distributed across glandular trichome transcribed genes and untranscribed genes.
Anti H3k56ac, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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anti h3k56ac - by Bioz Stars, 2024-07
86/100 stars

Images

1) Product Images from "Characterization of the Cannabis sativa glandular trichome epigenome"

Article Title: Characterization of the Cannabis sativa glandular trichome epigenome

Journal: bioRxiv

doi: 10.1101/2024.07.04.602151

A Light microscopy image (1 mm scale bar) of isolated C. glandular trichomes. B Spearman correlation matrix of RNA-seq and ChIP-seq data for H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. C C. sativa karyotype with glandular trichome data density plots (i) all genes (ii) transcribed genes (iii) H3K4me3 peaks (iv) H3K56ac peaks (v) untranscribed genes (vi) H3K27me3 domains and (vii) H2A.Z domains. D H2A.Z, H3K27me3, H3K56ac, and H3K4me3 peak annotation. E Scale-region gene plots and associated heatmaps of glandular trichome H3K4me3, H3K56ac, H3K27me3, and H2A.Z reads distributed across glandular trichome transcribed genes and untranscribed genes.
Figure Legend Snippet: A Light microscopy image (1 mm scale bar) of isolated C. glandular trichomes. B Spearman correlation matrix of RNA-seq and ChIP-seq data for H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. C C. sativa karyotype with glandular trichome data density plots (i) all genes (ii) transcribed genes (iii) H3K4me3 peaks (iv) H3K56ac peaks (v) untranscribed genes (vi) H3K27me3 domains and (vii) H2A.Z domains. D H2A.Z, H3K27me3, H3K56ac, and H3K4me3 peak annotation. E Scale-region gene plots and associated heatmaps of glandular trichome H3K4me3, H3K56ac, H3K27me3, and H2A.Z reads distributed across glandular trichome transcribed genes and untranscribed genes.

Techniques Used: Light Microscopy, Isolation, RNA Sequencing Assay, ChIP-sequencing

IDR analysis of A H3K4me3 and B H3K56ac yielded 19,704 and 13,590 replicable peaks respectively in glandular trichomes. BEDTools intersect analysis of C H3K27me3 and D H2A.Z yielded 10,284 and 13,058 replicable domains respectively in glandular trichomes. E Upstart plot shows histone modification co-localisation events in the glandular trichome genome. F Biological processes of genes containing H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. G KEGG ontologies of genes containing co-localised H3K4me3-H3K27me3 in glandular trichomes.
Figure Legend Snippet: IDR analysis of A H3K4me3 and B H3K56ac yielded 19,704 and 13,590 replicable peaks respectively in glandular trichomes. BEDTools intersect analysis of C H3K27me3 and D H2A.Z yielded 10,284 and 13,058 replicable domains respectively in glandular trichomes. E Upstart plot shows histone modification co-localisation events in the glandular trichome genome. F Biological processes of genes containing H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. G KEGG ontologies of genes containing co-localised H3K4me3-H3K27me3 in glandular trichomes.

Techniques Used: Modification

Tissue specific analysis of RNA and chromatin type datasets yield comprehensive insight into Cannabis sativa glandular trichomes. Pairwise gene set enrichment analysis (GSE) leveraging A Leaf and B Stem RNA datasets. KEGG ontology analysis of glandular trichome specific chromatin types C H3K4me3 D H3K56ac E H3K4me3-H3K27me3 F H3K27me3 and G H2A.Z.
Figure Legend Snippet: Tissue specific analysis of RNA and chromatin type datasets yield comprehensive insight into Cannabis sativa glandular trichomes. Pairwise gene set enrichment analysis (GSE) leveraging A Leaf and B Stem RNA datasets. KEGG ontology analysis of glandular trichome specific chromatin types C H3K4me3 D H3K56ac E H3K4me3-H3K27me3 F H3K27me3 and G H2A.Z.

Techniques Used: Cannabis

A Monoterpene synthase gene cluster previously identified in Conneely et al. 2022 as CBDRx cluster no.17 shows glandular trichome specific gene expression and chromatin landscape. B Genome data viewer screenshot of the 342 Kbp region encompassing the cannabigerolic acid synthase / prenyltransferase gene cluster in C. sativa. The CBGAS cluster genes PT3 (LOC115713148), LOC115713171, LOC115722991, PT4(LOC115713185), CBGAS/PT1(LOC115713215), CBGAS/PT7(LOC115713205) show trichome specific expression and enrichment of H3K4me3 and H3K56ac at their promoter regions.
Figure Legend Snippet: A Monoterpene synthase gene cluster previously identified in Conneely et al. 2022 as CBDRx cluster no.17 shows glandular trichome specific gene expression and chromatin landscape. B Genome data viewer screenshot of the 342 Kbp region encompassing the cannabigerolic acid synthase / prenyltransferase gene cluster in C. sativa. The CBGAS cluster genes PT3 (LOC115713148), LOC115713171, LOC115722991, PT4(LOC115713185), CBGAS/PT1(LOC115713215), CBGAS/PT7(LOC115713205) show trichome specific expression and enrichment of H3K4me3 and H3K56ac at their promoter regions.

Techniques Used: Expressing


Figure Legend Snippet:

Techniques Used: Expressing


Structured Review

Millipore rabbit polyclonal anti h3k56ac

Rabbit Polyclonal Anti H3k56ac, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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rabbit polyclonal anti h3k56ac - by Bioz Stars, 2024-07
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1) Product Images from "Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication"

Article Title: Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication

Journal: iScience

doi: 10.1016/j.isci.2024.110144


Figure Legend Snippet:

Techniques Used: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

anti h3k56ac antibody  (Danaher Inc)


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    Danaher Inc anti h3k56ac antibody
    Combination of dpb3Δ and mcm2-3A mutations neutralizes single dpb3Δ or mcm2-3A mutants’ strand bias in transferring parental histone H3–H4 tetramers during DNA replication. ( A ) Procedure for monitoring the deposition of parental (H3K4me3) and newly synthesized <t>(H3K56ac)</t> histone H3 at early replication origins. ( B ) Snapshot of parental histone H3 (H3K4me3) eSPAN reads enrichment at leading and lagging strands at the early replication origin ARS1309 for wild-type (WT), dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains. The sequence reads were mapped to both the Watson strand (red) and the Crick strand (green) of the reference genome. ( C–F ) Top : heatmaps representing the bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 individual nucleosomes surrounding each of the 134 early DNA replication origins. Individual nucleosomes are represented by the circles at the top of the heatmaps, and their positions are indicated relative to the origin (−10 to + 10). Each row represents the average log 2 Watson/Crick ratio of H3K4me3 eSPAN sequence reads at one origin. Bottom : average bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 nucleosomes surrounding the 134 early replication origins.
    Anti H3k56ac Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Defective transfer of parental histone decreases frequency of homologous recombination by increasing free histone pools in budding yeast"

    Article Title: Defective transfer of parental histone decreases frequency of homologous recombination by increasing free histone pools in budding yeast

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae205

    Combination of dpb3Δ and mcm2-3A mutations neutralizes single dpb3Δ or mcm2-3A mutants’ strand bias in transferring parental histone H3–H4 tetramers during DNA replication. ( A ) Procedure for monitoring the deposition of parental (H3K4me3) and newly synthesized (H3K56ac) histone H3 at early replication origins. ( B ) Snapshot of parental histone H3 (H3K4me3) eSPAN reads enrichment at leading and lagging strands at the early replication origin ARS1309 for wild-type (WT), dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains. The sequence reads were mapped to both the Watson strand (red) and the Crick strand (green) of the reference genome. ( C–F ) Top : heatmaps representing the bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 individual nucleosomes surrounding each of the 134 early DNA replication origins. Individual nucleosomes are represented by the circles at the top of the heatmaps, and their positions are indicated relative to the origin (−10 to + 10). Each row represents the average log 2 Watson/Crick ratio of H3K4me3 eSPAN sequence reads at one origin. Bottom : average bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 nucleosomes surrounding the 134 early replication origins.
    Figure Legend Snippet: Combination of dpb3Δ and mcm2-3A mutations neutralizes single dpb3Δ or mcm2-3A mutants’ strand bias in transferring parental histone H3–H4 tetramers during DNA replication. ( A ) Procedure for monitoring the deposition of parental (H3K4me3) and newly synthesized (H3K56ac) histone H3 at early replication origins. ( B ) Snapshot of parental histone H3 (H3K4me3) eSPAN reads enrichment at leading and lagging strands at the early replication origin ARS1309 for wild-type (WT), dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains. The sequence reads were mapped to both the Watson strand (red) and the Crick strand (green) of the reference genome. ( C–F ) Top : heatmaps representing the bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 individual nucleosomes surrounding each of the 134 early DNA replication origins. Individual nucleosomes are represented by the circles at the top of the heatmaps, and their positions are indicated relative to the origin (−10 to + 10). Each row represents the average log 2 Watson/Crick ratio of H3K4me3 eSPAN sequence reads at one origin. Bottom : average bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 nucleosomes surrounding the 134 early replication origins.

    Techniques Used: Transferring, Synthesized, Sequencing

    rabbit monoclonal antibody anti h3k56ac  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal antibody anti h3k56ac

    Rabbit Monoclonal Antibody Anti H3k56ac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling"

    Article Title: Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2024.101510


    Figure Legend Snippet:

    Techniques Used: Microarray, Recombinant, Clone Assay, Mutagenesis, Immunohistochemistry, Protein Purification, In Vitro, Sequencing, Methylation


    Structured Review

    ABclonal Biotechnology anti h3k56ac antibody
    Anti H3k56ac Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Structured Review

    Millipore anti h3k56ac
    Nucleus-localized Sas3 possesses histone acetyltransferase activity. ( A ) Colony morphologies of the wild-type and Sas3-GFP strains grown on MM and YG media at 37°C for 48 h. ( B ) Western blot analysis of Sas3-GFP strain. β-Actin served as a loading control. ( C ) Localization of Sas3-GFP in vegetative stages of fungal development. Nuclei were stained with Hoechst dye 33258. Scale bar = 10 µm. ( D ) Western blotting for acetylated H3K9, H3K14, and H3K56 in the wild-type and Δ sas3 mutants. H3 served as a loading control. ( E ) Quantified analysis of Western blot signal for H3K14ac, H3K9ac, and <t>H3K56ac</t> relative to H3 in the indicated strains. Data from three independent experiments are presented as mean ± SD. Statistical analysis was performed using two-tailed, unpaired t -tests. ** P < 0.01. H3, histone H3; MAPK, mitogen-activated protein kinase; ns, not significant.
    Anti H3k56ac, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Histone acetyltransferase Sas3 contributes to fungal development, cell wall integrity, and virulence in Aspergillus fumigatus"

    Article Title: Histone acetyltransferase Sas3 contributes to fungal development, cell wall integrity, and virulence in Aspergillus fumigatus

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/aem.01885-23

    Nucleus-localized Sas3 possesses histone acetyltransferase activity. ( A ) Colony morphologies of the wild-type and Sas3-GFP strains grown on MM and YG media at 37°C for 48 h. ( B ) Western blot analysis of Sas3-GFP strain. β-Actin served as a loading control. ( C ) Localization of Sas3-GFP in vegetative stages of fungal development. Nuclei were stained with Hoechst dye 33258. Scale bar = 10 µm. ( D ) Western blotting for acetylated H3K9, H3K14, and H3K56 in the wild-type and Δ sas3 mutants. H3 served as a loading control. ( E ) Quantified analysis of Western blot signal for H3K14ac, H3K9ac, and H3K56ac relative to H3 in the indicated strains. Data from three independent experiments are presented as mean ± SD. Statistical analysis was performed using two-tailed, unpaired t -tests. ** P < 0.01. H3, histone H3; MAPK, mitogen-activated protein kinase; ns, not significant.
    Figure Legend Snippet: Nucleus-localized Sas3 possesses histone acetyltransferase activity. ( A ) Colony morphologies of the wild-type and Sas3-GFP strains grown on MM and YG media at 37°C for 48 h. ( B ) Western blot analysis of Sas3-GFP strain. β-Actin served as a loading control. ( C ) Localization of Sas3-GFP in vegetative stages of fungal development. Nuclei were stained with Hoechst dye 33258. Scale bar = 10 µm. ( D ) Western blotting for acetylated H3K9, H3K14, and H3K56 in the wild-type and Δ sas3 mutants. H3 served as a loading control. ( E ) Quantified analysis of Western blot signal for H3K14ac, H3K9ac, and H3K56ac relative to H3 in the indicated strains. Data from three independent experiments are presented as mean ± SD. Statistical analysis was performed using two-tailed, unpaired t -tests. ** P < 0.01. H3, histone H3; MAPK, mitogen-activated protein kinase; ns, not significant.

    Techniques Used: Activity Assay, Western Blot, Staining, Two Tailed Test

    anti h3k56ac antibody  (Danaher Inc)


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    Danaher Inc anti h3k56ac antibody
    Anti H3k56ac Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti h3k56ac antibody - by Bioz Stars, 2024-07
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    Huabio Inc h3k56ac
    H3k56ac, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit monoclonal anti histone h3k56ac antibodies  (Danaher Inc)


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    Danaher Inc rabbit monoclonal anti histone h3k56ac antibodies
    Rabbit Monoclonal Anti Histone H3k56ac Antibodies, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit monoclonal anti histone h3k56ac antibodies - by Bioz Stars, 2024-07
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    Millipore anti h3k56ac
    Anti H3k56ac, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A Light microscopy image (1 mm scale bar) of isolated C. glandular trichomes. B Spearman correlation matrix of RNA-seq and ChIP-seq data for H3K4me3, <t>H3K56ac,</t> H3K27me3, and H2A.Z in glandular trichomes. C C. sativa karyotype with glandular trichome data density plots (i) all genes (ii) transcribed genes (iii) H3K4me3 peaks (iv) H3K56ac peaks (v) untranscribed genes (vi) H3K27me3 domains and (vii) H2A.Z domains. D H2A.Z, H3K27me3, H3K56ac, and H3K4me3 peak annotation. E Scale-region gene plots and associated heatmaps of glandular trichome H3K4me3, H3K56ac, H3K27me3, and H2A.Z reads distributed across glandular trichome transcribed genes and untranscribed genes.
    Anti H3k56ac, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Combination of dpb3Δ and mcm2-3A mutations neutralizes single dpb3Δ or mcm2-3A mutants’ strand bias in transferring parental histone H3–H4 tetramers during DNA replication. ( A ) Procedure for monitoring the deposition of parental (H3K4me3) and newly synthesized <t>(H3K56ac)</t> histone H3 at early replication origins. ( B ) Snapshot of parental histone H3 (H3K4me3) eSPAN reads enrichment at leading and lagging strands at the early replication origin ARS1309 for wild-type (WT), dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains. The sequence reads were mapped to both the Watson strand (red) and the Crick strand (green) of the reference genome. ( C–F ) Top : heatmaps representing the bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 individual nucleosomes surrounding each of the 134 early DNA replication origins. Individual nucleosomes are represented by the circles at the top of the heatmaps, and their positions are indicated relative to the origin (−10 to + 10). Each row represents the average log 2 Watson/Crick ratio of H3K4me3 eSPAN sequence reads at one origin. Bottom : average bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 nucleosomes surrounding the 134 early replication origins.
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    A Light microscopy image (1 mm scale bar) of isolated C. glandular trichomes. B Spearman correlation matrix of RNA-seq and ChIP-seq data for H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. C C. sativa karyotype with glandular trichome data density plots (i) all genes (ii) transcribed genes (iii) H3K4me3 peaks (iv) H3K56ac peaks (v) untranscribed genes (vi) H3K27me3 domains and (vii) H2A.Z domains. D H2A.Z, H3K27me3, H3K56ac, and H3K4me3 peak annotation. E Scale-region gene plots and associated heatmaps of glandular trichome H3K4me3, H3K56ac, H3K27me3, and H2A.Z reads distributed across glandular trichome transcribed genes and untranscribed genes.

    Journal: bioRxiv

    Article Title: Characterization of the Cannabis sativa glandular trichome epigenome

    doi: 10.1101/2024.07.04.602151

    Figure Lengend Snippet: A Light microscopy image (1 mm scale bar) of isolated C. glandular trichomes. B Spearman correlation matrix of RNA-seq and ChIP-seq data for H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. C C. sativa karyotype with glandular trichome data density plots (i) all genes (ii) transcribed genes (iii) H3K4me3 peaks (iv) H3K56ac peaks (v) untranscribed genes (vi) H3K27me3 domains and (vii) H2A.Z domains. D H2A.Z, H3K27me3, H3K56ac, and H3K4me3 peak annotation. E Scale-region gene plots and associated heatmaps of glandular trichome H3K4me3, H3K56ac, H3K27me3, and H2A.Z reads distributed across glandular trichome transcribed genes and untranscribed genes.

    Article Snippet: Eppendorf tubes were labelled with the appropriate sample name and histone mark and 60 µL aliquots of the washed Dynabeads-Protein A were made into each tube followed by the addition of 2.5 µL (2.5 µg) of either Anti-H3K4me3 (Millipore®, 07-473), Anti-H3K56ac (Millipore®, 07-677-1), Anti-H3K27me3 (Millipore®, 07-449), or Anti-H2A.Z (Millipore®, 07-594) to their correspondingly labelled tubes.

    Techniques: Light Microscopy, Isolation, RNA Sequencing Assay, ChIP-sequencing

    IDR analysis of A H3K4me3 and B H3K56ac yielded 19,704 and 13,590 replicable peaks respectively in glandular trichomes. BEDTools intersect analysis of C H3K27me3 and D H2A.Z yielded 10,284 and 13,058 replicable domains respectively in glandular trichomes. E Upstart plot shows histone modification co-localisation events in the glandular trichome genome. F Biological processes of genes containing H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. G KEGG ontologies of genes containing co-localised H3K4me3-H3K27me3 in glandular trichomes.

    Journal: bioRxiv

    Article Title: Characterization of the Cannabis sativa glandular trichome epigenome

    doi: 10.1101/2024.07.04.602151

    Figure Lengend Snippet: IDR analysis of A H3K4me3 and B H3K56ac yielded 19,704 and 13,590 replicable peaks respectively in glandular trichomes. BEDTools intersect analysis of C H3K27me3 and D H2A.Z yielded 10,284 and 13,058 replicable domains respectively in glandular trichomes. E Upstart plot shows histone modification co-localisation events in the glandular trichome genome. F Biological processes of genes containing H3K4me3, H3K56ac, H3K27me3, and H2A.Z in glandular trichomes. G KEGG ontologies of genes containing co-localised H3K4me3-H3K27me3 in glandular trichomes.

    Article Snippet: Eppendorf tubes were labelled with the appropriate sample name and histone mark and 60 µL aliquots of the washed Dynabeads-Protein A were made into each tube followed by the addition of 2.5 µL (2.5 µg) of either Anti-H3K4me3 (Millipore®, 07-473), Anti-H3K56ac (Millipore®, 07-677-1), Anti-H3K27me3 (Millipore®, 07-449), or Anti-H2A.Z (Millipore®, 07-594) to their correspondingly labelled tubes.

    Techniques: Modification

    Tissue specific analysis of RNA and chromatin type datasets yield comprehensive insight into Cannabis sativa glandular trichomes. Pairwise gene set enrichment analysis (GSE) leveraging A Leaf and B Stem RNA datasets. KEGG ontology analysis of glandular trichome specific chromatin types C H3K4me3 D H3K56ac E H3K4me3-H3K27me3 F H3K27me3 and G H2A.Z.

    Journal: bioRxiv

    Article Title: Characterization of the Cannabis sativa glandular trichome epigenome

    doi: 10.1101/2024.07.04.602151

    Figure Lengend Snippet: Tissue specific analysis of RNA and chromatin type datasets yield comprehensive insight into Cannabis sativa glandular trichomes. Pairwise gene set enrichment analysis (GSE) leveraging A Leaf and B Stem RNA datasets. KEGG ontology analysis of glandular trichome specific chromatin types C H3K4me3 D H3K56ac E H3K4me3-H3K27me3 F H3K27me3 and G H2A.Z.

    Article Snippet: Eppendorf tubes were labelled with the appropriate sample name and histone mark and 60 µL aliquots of the washed Dynabeads-Protein A were made into each tube followed by the addition of 2.5 µL (2.5 µg) of either Anti-H3K4me3 (Millipore®, 07-473), Anti-H3K56ac (Millipore®, 07-677-1), Anti-H3K27me3 (Millipore®, 07-449), or Anti-H2A.Z (Millipore®, 07-594) to their correspondingly labelled tubes.

    Techniques: Cannabis

    A Monoterpene synthase gene cluster previously identified in Conneely et al. 2022 as CBDRx cluster no.17 shows glandular trichome specific gene expression and chromatin landscape. B Genome data viewer screenshot of the 342 Kbp region encompassing the cannabigerolic acid synthase / prenyltransferase gene cluster in C. sativa. The CBGAS cluster genes PT3 (LOC115713148), LOC115713171, LOC115722991, PT4(LOC115713185), CBGAS/PT1(LOC115713215), CBGAS/PT7(LOC115713205) show trichome specific expression and enrichment of H3K4me3 and H3K56ac at their promoter regions.

    Journal: bioRxiv

    Article Title: Characterization of the Cannabis sativa glandular trichome epigenome

    doi: 10.1101/2024.07.04.602151

    Figure Lengend Snippet: A Monoterpene synthase gene cluster previously identified in Conneely et al. 2022 as CBDRx cluster no.17 shows glandular trichome specific gene expression and chromatin landscape. B Genome data viewer screenshot of the 342 Kbp region encompassing the cannabigerolic acid synthase / prenyltransferase gene cluster in C. sativa. The CBGAS cluster genes PT3 (LOC115713148), LOC115713171, LOC115722991, PT4(LOC115713185), CBGAS/PT1(LOC115713215), CBGAS/PT7(LOC115713205) show trichome specific expression and enrichment of H3K4me3 and H3K56ac at their promoter regions.

    Article Snippet: Eppendorf tubes were labelled with the appropriate sample name and histone mark and 60 µL aliquots of the washed Dynabeads-Protein A were made into each tube followed by the addition of 2.5 µL (2.5 µg) of either Anti-H3K4me3 (Millipore®, 07-473), Anti-H3K56ac (Millipore®, 07-677-1), Anti-H3K27me3 (Millipore®, 07-449), or Anti-H2A.Z (Millipore®, 07-594) to their correspondingly labelled tubes.

    Techniques: Expressing

    Journal: bioRxiv

    Article Title: Characterization of the Cannabis sativa glandular trichome epigenome

    doi: 10.1101/2024.07.04.602151

    Figure Lengend Snippet:

    Article Snippet: Eppendorf tubes were labelled with the appropriate sample name and histone mark and 60 µL aliquots of the washed Dynabeads-Protein A were made into each tube followed by the addition of 2.5 µL (2.5 µg) of either Anti-H3K4me3 (Millipore®, 07-473), Anti-H3K56ac (Millipore®, 07-677-1), Anti-H3K27me3 (Millipore®, 07-449), or Anti-H2A.Z (Millipore®, 07-594) to their correspondingly labelled tubes.

    Techniques: Expressing

    Journal: iScience

    Article Title: Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication

    doi: 10.1016/j.isci.2024.110144

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-H3K56ac , Millipore , Cat# 07-677 RRID: AB_390167.

    Techniques: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

    Combination of dpb3Δ and mcm2-3A mutations neutralizes single dpb3Δ or mcm2-3A mutants’ strand bias in transferring parental histone H3–H4 tetramers during DNA replication. ( A ) Procedure for monitoring the deposition of parental (H3K4me3) and newly synthesized (H3K56ac) histone H3 at early replication origins. ( B ) Snapshot of parental histone H3 (H3K4me3) eSPAN reads enrichment at leading and lagging strands at the early replication origin ARS1309 for wild-type (WT), dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains. The sequence reads were mapped to both the Watson strand (red) and the Crick strand (green) of the reference genome. ( C–F ) Top : heatmaps representing the bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 individual nucleosomes surrounding each of the 134 early DNA replication origins. Individual nucleosomes are represented by the circles at the top of the heatmaps, and their positions are indicated relative to the origin (−10 to + 10). Each row represents the average log 2 Watson/Crick ratio of H3K4me3 eSPAN sequence reads at one origin. Bottom : average bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 nucleosomes surrounding the 134 early replication origins.

    Journal: Nucleic Acids Research

    Article Title: Defective transfer of parental histone decreases frequency of homologous recombination by increasing free histone pools in budding yeast

    doi: 10.1093/nar/gkae205

    Figure Lengend Snippet: Combination of dpb3Δ and mcm2-3A mutations neutralizes single dpb3Δ or mcm2-3A mutants’ strand bias in transferring parental histone H3–H4 tetramers during DNA replication. ( A ) Procedure for monitoring the deposition of parental (H3K4me3) and newly synthesized (H3K56ac) histone H3 at early replication origins. ( B ) Snapshot of parental histone H3 (H3K4me3) eSPAN reads enrichment at leading and lagging strands at the early replication origin ARS1309 for wild-type (WT), dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains. The sequence reads were mapped to both the Watson strand (red) and the Crick strand (green) of the reference genome. ( C–F ) Top : heatmaps representing the bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 individual nucleosomes surrounding each of the 134 early DNA replication origins. Individual nucleosomes are represented by the circles at the top of the heatmaps, and their positions are indicated relative to the origin (−10 to + 10). Each row represents the average log 2 Watson/Crick ratio of H3K4me3 eSPAN sequence reads at one origin. Bottom : average bias ratio of parental histone H3 (H3K4me3) eSPAN peaks for WT, dpb3Δ , mcm2-3A and dpb3Δ mcm2-3A strains at each of the 10 nucleosomes surrounding the 134 early replication origins.

    Article Snippet: Soluble chromatin (eSPAN samples) was immunoprecipitated with anti-H3K4me3 antibody (ab8580 Abcam) or anti-H3K56ac antibody ( ).

    Techniques: Transferring, Synthesized, Sequencing

    Journal: Cell Reports Medicine

    Article Title: Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling

    doi: 10.1016/j.xcrm.2024.101510

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal antibody anti-H3K56ac , Cell Signaling Technology , Cat# 9927; Acetyl-Histone H3 Antibody Sampler Kit.

    Techniques: Microarray, Recombinant, Clone Assay, Mutagenesis, Immunohistochemistry, Protein Purification, In Vitro, Sequencing, Methylation