h2 o2 (Thermo Fisher)

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Thermo Fisher h2 o2
H2 O2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2 o2/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

h2 o2 - by Bioz Stars, 2021-03

86/100 stars

Images

Luminescence Assay:

Article Title: Reactive Dicarbonyl Scavenging Effectively Reduces MPO-Mediated Oxidation of HDL and Restores PON1 Activity
Article Snippet: .. Buffers were used in L-012 luminescence assay, including PS6.5 buffer (130 mM NaCl, 6.28 mM Na2 HPO4 , 18.7 mM NaH2 PO4 ), and MAPS imaging solution (buffer Cit6 with 20 mM NaBr, 200 mM (NH4 )2 SO4 , 100 p.p.m. v/v Tween20, 50 μM L-012 and 100 μM (H2 O2), 1 ppm = 0.0001%)) [ , ]. .. Mice—Apoe−/− and Ldlr−/− mice on a C57BL/6 background were purchased from Jackson Laboratories (Bar Harbor, ME).

Article Title: Reactive Dicarbonyl Scavenging Effectively Reduces MPO-Mediated Oxidation of HDL and Preserves HDL Atheroprotective Functions
Article Snippet: Working solutions of H2 O2 were made fresh daily by diluting 30% H2 O2 (BDH Chemicals, London, UK) according to the extinction coefficient for H2 O2 at 240 nm, 39.4 M-1 cm-1 [ ]. .. Buffers were used in L-012 luminescence assay, including PS6.5 buffer (130mM NaCl, 6.28mM Na2 HPO4 , 18.7mM NaH2 PO4 ), and MAPS imaging solution (buffer Cit6 with 20mM NaBr, 200mM (NH4 )2 SO4 , 100 p.p.m. v/v Tween20, 50 μM L-012 and 100 μM (H2 O2), 1ppm = 0.0001%)) [ , ]. .. Mice Apoe -/- and Ldlr -/- mice were purchased from Jackson Laboratories (Bar Harbor, ME).

Imaging:

other:

Article Title: Spatial Positioning of All 24 Chromosomes in the Lymphocytes of Six Subjects: Evidence of Reproducible Positioning and Spatial Repositioning following DNA Damage with Hydrogen Peroxide and Ultraviolet B
Article Snippet: Additionally, the chi-squared goodness-of-fit test (χ2 ) was used to evaluate whether CT repositioning occurred in the H2 O2 and UVB exposed cultures by comparing the CT distribution in the unexposed culture from the same individual, (p > 0.05 provided evidence of no change in CT distribution following exposure, whereas p < 0.05 provided evidence of altered CT positioning).

Article Title: Evaluation of the protective effects of quercetin and gallic acid against oxidative toxicity in rat’s kidney and HEK-293 cells
Article Snippet: Thereafter, the cells were seeded in 6-wells plate (Nunc International, Rochester, USA) at a density of 2 x 105 cells per well, and were allowed to reach 90-100 % confluence in 48-72 h. After pre-treatment of the cells with GAL (25 or 49 μM) or QUE (2-17 μM or 28-165.43 μM) for 1 h followed by treatment with 200 μM H2 O2 [ , , , , , ] for 2, 6 or 12 h, the cells were scraped from the culture plates, centrifuged at 5000 x g for 10 min and washed with phosphate buffer (pH 7.4).

Article Title: Spatial Positioning of All 24 Chromosomes in the Lymphocytes of Six Subjects: Evidence of Reproducible Positioning and Spatial Repositioning following DNA Damage with Hydrogen Peroxide and Ultraviolet B
Article Snippet: Seven CTs were involved in a statistically significant alteration of their position that were common in both H2 O2 and UVB treated cells (4, 8, 10, 12, 17, 19, and X), whereas repositioning of chromosomes 6, 7, and 14 was exclusive to H2 O2 and chromosomes 15 and 22 to UVB exposure.

Immunohistochemistry:

Article Title: A Novel Role for GATA3 in Mesangial Cells in Glomerular Development and Injury
Article Snippet: .. For immunohistochemistry (IHC) signal detection, endogenous peroxidase activity was quenched by incubation in 3% (vol/vol) H2 O2). .. For IHC detection, the immobilized antibodies were detected by UltraVision-LP HRP Polymer detection system specific for anti-mouse and anti-rabbit IgG (ThermoFisher Scientific), or with biotinylated secondary antibodies and AB reagents (Vector Laboratories) according to manufacturer’s instructions.

Activity Assay:

Incubation:

Inhibition:

Article Title: A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells
Article Snippet: All mutations resulted in frameshifts and early stop codons that generated knockouts of the targeted genes. .. Cytotoxicity assays for H2 O2 with caspase inhibition and iron chelation. .. WT HAP1 cells (1.5 × 105 cells per well) were seeded in 12-well plates and incubated for 24 h at 37°C in 5% CO2 .

Confocal Microscopy:

Article Title: Mitochondrial Superoxide Production Decreases on Glucose-Stimulated Insulin Secretion in Pancreatic β Cells Due to Decreasing Mitochondrial Matrix NADH/NAD+ Ratio
Article Snippet: In this way, snapshots of superoxide release rates are obtained, which provide insights into important physiological phenomena such as the redox initiation of the hypoxia-inducible factor signaling, that is, the peak in J m rates occurring after 5 h of hypoxic incubation (Plecitá et al., unpublished observations). .. Confocal microscopy assay of H2 O2 release into the mitochondrial matrix within intact cells The HyPer family of fluorescence probes has been developed for the selective detection of H2 O2 ( , , , ). ..