h2 o2  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher h2 o2
    H2 O2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars

    Images

    Related Articles

    Luminescence Assay:

    Article Title: Reactive Dicarbonyl Scavenging Effectively Reduces MPO-Mediated Oxidation of HDL and Restores PON1 Activity
    Article Snippet: .. Buffers were used in L-012 luminescence assay, including PS6.5 buffer (130 mM NaCl, 6.28 mM Na2 HPO4 , 18.7 mM NaH2 PO4 ), and MAPS imaging solution (buffer Cit6 with 20 mM NaBr, 200 mM (NH4 )2 SO4 , 100 p.p.m. v/v Tween20, 50 μM L-012 and 100 μM (H2 O2), 1 ppm = 0.0001%)) [ , ]. .. Mice—Apoe−/− and Ldlr−/− mice on a C57BL/6 background were purchased from Jackson Laboratories (Bar Harbor, ME).

    Article Title: Reactive Dicarbonyl Scavenging Effectively Reduces MPO-Mediated Oxidation of HDL and Preserves HDL Atheroprotective Functions
    Article Snippet: Working solutions of H2 O2 were made fresh daily by diluting 30% H2 O2 (BDH Chemicals, London, UK) according to the extinction coefficient for H2 O2 at 240 nm, 39.4 M-1 cm-1 [ ]. .. Buffers were used in L-012 luminescence assay, including PS6.5 buffer (130mM NaCl, 6.28mM Na2 HPO4 , 18.7mM NaH2 PO4 ), and MAPS imaging solution (buffer Cit6 with 20mM NaBr, 200mM (NH4 )2 SO4 , 100 p.p.m. v/v Tween20, 50 μM L-012 and 100 μM (H2 O2), 1ppm = 0.0001%)) [ , ]. .. Mice Apoe -/- and Ldlr -/- mice were purchased from Jackson Laboratories (Bar Harbor, ME).

    Imaging:

    Article Title: Reactive Dicarbonyl Scavenging Effectively Reduces MPO-Mediated Oxidation of HDL and Restores PON1 Activity
    Article Snippet: .. Buffers were used in L-012 luminescence assay, including PS6.5 buffer (130 mM NaCl, 6.28 mM Na2 HPO4 , 18.7 mM NaH2 PO4 ), and MAPS imaging solution (buffer Cit6 with 20 mM NaBr, 200 mM (NH4 )2 SO4 , 100 p.p.m. v/v Tween20, 50 μM L-012 and 100 μM (H2 O2), 1 ppm = 0.0001%)) [ , ]. .. Mice—Apoe−/− and Ldlr−/− mice on a C57BL/6 background were purchased from Jackson Laboratories (Bar Harbor, ME).

    Article Title: Reactive Dicarbonyl Scavenging Effectively Reduces MPO-Mediated Oxidation of HDL and Preserves HDL Atheroprotective Functions
    Article Snippet: Working solutions of H2 O2 were made fresh daily by diluting 30% H2 O2 (BDH Chemicals, London, UK) according to the extinction coefficient for H2 O2 at 240 nm, 39.4 M-1 cm-1 [ ]. .. Buffers were used in L-012 luminescence assay, including PS6.5 buffer (130mM NaCl, 6.28mM Na2 HPO4 , 18.7mM NaH2 PO4 ), and MAPS imaging solution (buffer Cit6 with 20mM NaBr, 200mM (NH4 )2 SO4 , 100 p.p.m. v/v Tween20, 50 μM L-012 and 100 μM (H2 O2), 1ppm = 0.0001%)) [ , ]. .. Mice Apoe -/- and Ldlr -/- mice were purchased from Jackson Laboratories (Bar Harbor, ME).

    other:

    Article Title: Spatial Positioning of All 24 Chromosomes in the Lymphocytes of Six Subjects: Evidence of Reproducible Positioning and Spatial Repositioning following DNA Damage with Hydrogen Peroxide and Ultraviolet B
    Article Snippet: Additionally, the chi-squared goodness-of-fit test (χ2 ) was used to evaluate whether CT repositioning occurred in the H2 O2 and UVB exposed cultures by comparing the CT distribution in the unexposed culture from the same individual, (p > 0.05 provided evidence of no change in CT distribution following exposure, whereas p < 0.05 provided evidence of altered CT positioning).

    Article Title: Evaluation of the protective effects of quercetin and gallic acid against oxidative toxicity in rat’s kidney and HEK-293 cells
    Article Snippet: Thereafter, the cells were seeded in 6-wells plate (Nunc International, Rochester, USA) at a density of 2 x 105 cells per well, and were allowed to reach 90-100 % confluence in 48-72 h. After pre-treatment of the cells with GAL (25 or 49 μM) or QUE (2-17 μM or 28-165.43 μM) for 1 h followed by treatment with 200 μM H2 O2 [ , , , , , ] for 2, 6 or 12 h, the cells were scraped from the culture plates, centrifuged at 5000 x g for 10 min and washed with phosphate buffer (pH 7.4).

    Article Title: Spatial Positioning of All 24 Chromosomes in the Lymphocytes of Six Subjects: Evidence of Reproducible Positioning and Spatial Repositioning following DNA Damage with Hydrogen Peroxide and Ultraviolet B
    Article Snippet: Seven CTs were involved in a statistically significant alteration of their position that were common in both H2 O2 and UVB treated cells (4, 8, 10, 12, 17, 19, and X), whereas repositioning of chromosomes 6, 7, and 14 was exclusive to H2 O2 and chromosomes 15 and 22 to UVB exposure.

    Immunohistochemistry:

    Article Title: A Novel Role for GATA3 in Mesangial Cells in Glomerular Development and Injury
    Article Snippet: .. For immunohistochemistry (IHC) signal detection, endogenous peroxidase activity was quenched by incubation in 3% (vol/vol) H2 O2). .. For IHC detection, the immobilized antibodies were detected by UltraVision-LP HRP Polymer detection system specific for anti-mouse and anti-rabbit IgG (ThermoFisher Scientific), or with biotinylated secondary antibodies and AB reagents (Vector Laboratories) according to manufacturer’s instructions.

    Activity Assay:

    Article Title: A Novel Role for GATA3 in Mesangial Cells in Glomerular Development and Injury
    Article Snippet: .. For immunohistochemistry (IHC) signal detection, endogenous peroxidase activity was quenched by incubation in 3% (vol/vol) H2 O2). .. For IHC detection, the immobilized antibodies were detected by UltraVision-LP HRP Polymer detection system specific for anti-mouse and anti-rabbit IgG (ThermoFisher Scientific), or with biotinylated secondary antibodies and AB reagents (Vector Laboratories) according to manufacturer’s instructions.

    Incubation:

    Article Title: A Novel Role for GATA3 in Mesangial Cells in Glomerular Development and Injury
    Article Snippet: .. For immunohistochemistry (IHC) signal detection, endogenous peroxidase activity was quenched by incubation in 3% (vol/vol) H2 O2). .. For IHC detection, the immobilized antibodies were detected by UltraVision-LP HRP Polymer detection system specific for anti-mouse and anti-rabbit IgG (ThermoFisher Scientific), or with biotinylated secondary antibodies and AB reagents (Vector Laboratories) according to manufacturer’s instructions.

    Inhibition:

    Article Title: A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells
    Article Snippet: All mutations resulted in frameshifts and early stop codons that generated knockouts of the targeted genes. .. Cytotoxicity assays for H2 O2 with caspase inhibition and iron chelation. .. WT HAP1 cells (1.5 × 105 cells per well) were seeded in 12-well plates and incubated for 24 h at 37°C in 5% CO2 .

    Confocal Microscopy:

    Article Title: Mitochondrial Superoxide Production Decreases on Glucose-Stimulated Insulin Secretion in Pancreatic β Cells Due to Decreasing Mitochondrial Matrix NADH/NAD+ Ratio
    Article Snippet: In this way, snapshots of superoxide release rates are obtained, which provide insights into important physiological phenomena such as the redox initiation of the hypoxia-inducible factor signaling, that is, the peak in J m rates occurring after 5 h of hypoxic incubation (Plecitá et al., unpublished observations). .. Confocal microscopy assay of H2 O2 release into the mitochondrial matrix within intact cells The HyPer family of fluorescence probes has been developed for the selective detection of H2 O2 ( , , , ). ..

    Fluorescence:

    Article Title: Mitochondrial Superoxide Production Decreases on Glucose-Stimulated Insulin Secretion in Pancreatic β Cells Due to Decreasing Mitochondrial Matrix NADH/NAD+ Ratio
    Article Snippet: In this way, snapshots of superoxide release rates are obtained, which provide insights into important physiological phenomena such as the redox initiation of the hypoxia-inducible factor signaling, that is, the peak in J m rates occurring after 5 h of hypoxic incubation (Plecitá et al., unpublished observations). .. Confocal microscopy assay of H2 O2 release into the mitochondrial matrix within intact cells The HyPer family of fluorescence probes has been developed for the selective detection of H2 O2 ( , , , ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Thermo Fisher cellrox
    Reintroduction of Nrf2 rescues the Mst1/2-null phenotype. a Flow cytometry analyzing of ROS levels in Mst1 fl/fl Mst2 fl/fl (WT) BMDMs, Mst1 fl/fl Mst2 fl/fl Lyz -Cre (DKO) BMDMs, or DKO BMDMs infected with adenovirus expressing GFP (Ad-GFP) or Nrf2 (Ad-Nrf2), with the <t>CellRox</t> dye and quantification of the relative fluorescence intensity in the indicated cell samples shown in the right panel. b – e RT-qPCR analysis of the mRNA levels of the antioxidant genes Ho-1 and Nqo-1 ( b ), fluorescence microscopy of p-H2A.X (red), DAPI-stained nuclei (blue) and GFP + cells (green) ( c ), Annexin V/DAPI staining ( d ) and quantification of Annexin V + DAPI − cells ( e ) in WT BMDMs, DKO BMDMs, or DKO BMDMs infected with Ad-GFP or Ad-Nrf2, followed with or without H 2 O 2 treatment as indicated. Scale bars, 20 μm. f Relative fluorescence intensities of telomere FISH of GFP + peritoneal macrophages isolated from WT mice transplanted for 6 months with DKO bone marrow cells, which were transduced with retrovirus expressing Nrf2, or control GFP, respectively. g A proposed working model for kinases Mst1/2 sense ROS and maintain cellular redox balance by modulating the stability of Nrf2. Phagosomal or mitochondrial ROS release attracts Mst1/2 to cap around phagosome or mitochondrion from the cytosol and activates Mst1/2; Mst1/2 phosphorylate Keap1 to stabilize Nrf2 and regulate the expression of antioxidant enzymes to protect cell against oxidative damage. Data are from one experiment representative of three independent experiments with similar results. ns, not significant ( P > 0.05); * P
    Cellrox, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellrox/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellrox - by Bioz Stars, 2021-03
    98/100 stars
      Buy from Supplier

    95
    Thermo Fisher h2 o2
    Genes identified from a positive-selection CRISPR/Cas9 screen using <t>H2</t> O2 .
    H2 O2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    Image Search Results


    Reintroduction of Nrf2 rescues the Mst1/2-null phenotype. a Flow cytometry analyzing of ROS levels in Mst1 fl/fl Mst2 fl/fl (WT) BMDMs, Mst1 fl/fl Mst2 fl/fl Lyz -Cre (DKO) BMDMs, or DKO BMDMs infected with adenovirus expressing GFP (Ad-GFP) or Nrf2 (Ad-Nrf2), with the CellRox dye and quantification of the relative fluorescence intensity in the indicated cell samples shown in the right panel. b – e RT-qPCR analysis of the mRNA levels of the antioxidant genes Ho-1 and Nqo-1 ( b ), fluorescence microscopy of p-H2A.X (red), DAPI-stained nuclei (blue) and GFP + cells (green) ( c ), Annexin V/DAPI staining ( d ) and quantification of Annexin V + DAPI − cells ( e ) in WT BMDMs, DKO BMDMs, or DKO BMDMs infected with Ad-GFP or Ad-Nrf2, followed with or without H 2 O 2 treatment as indicated. Scale bars, 20 μm. f Relative fluorescence intensities of telomere FISH of GFP + peritoneal macrophages isolated from WT mice transplanted for 6 months with DKO bone marrow cells, which were transduced with retrovirus expressing Nrf2, or control GFP, respectively. g A proposed working model for kinases Mst1/2 sense ROS and maintain cellular redox balance by modulating the stability of Nrf2. Phagosomal or mitochondrial ROS release attracts Mst1/2 to cap around phagosome or mitochondrion from the cytosol and activates Mst1/2; Mst1/2 phosphorylate Keap1 to stabilize Nrf2 and regulate the expression of antioxidant enzymes to protect cell against oxidative damage. Data are from one experiment representative of three independent experiments with similar results. ns, not significant ( P > 0.05); * P

    Journal: Nature Communications

    Article Title: Macrophage achieves self-protection against oxidative stress-induced ageing through the Mst-Nrf2 axis

    doi: 10.1038/s41467-019-08680-6

    Figure Lengend Snippet: Reintroduction of Nrf2 rescues the Mst1/2-null phenotype. a Flow cytometry analyzing of ROS levels in Mst1 fl/fl Mst2 fl/fl (WT) BMDMs, Mst1 fl/fl Mst2 fl/fl Lyz -Cre (DKO) BMDMs, or DKO BMDMs infected with adenovirus expressing GFP (Ad-GFP) or Nrf2 (Ad-Nrf2), with the CellRox dye and quantification of the relative fluorescence intensity in the indicated cell samples shown in the right panel. b – e RT-qPCR analysis of the mRNA levels of the antioxidant genes Ho-1 and Nqo-1 ( b ), fluorescence microscopy of p-H2A.X (red), DAPI-stained nuclei (blue) and GFP + cells (green) ( c ), Annexin V/DAPI staining ( d ) and quantification of Annexin V + DAPI − cells ( e ) in WT BMDMs, DKO BMDMs, or DKO BMDMs infected with Ad-GFP or Ad-Nrf2, followed with or without H 2 O 2 treatment as indicated. Scale bars, 20 μm. f Relative fluorescence intensities of telomere FISH of GFP + peritoneal macrophages isolated from WT mice transplanted for 6 months with DKO bone marrow cells, which were transduced with retrovirus expressing Nrf2, or control GFP, respectively. g A proposed working model for kinases Mst1/2 sense ROS and maintain cellular redox balance by modulating the stability of Nrf2. Phagosomal or mitochondrial ROS release attracts Mst1/2 to cap around phagosome or mitochondrion from the cytosol and activates Mst1/2; Mst1/2 phosphorylate Keap1 to stabilize Nrf2 and regulate the expression of antioxidant enzymes to protect cell against oxidative damage. Data are from one experiment representative of three independent experiments with similar results. ns, not significant ( P > 0.05); * P

    Article Snippet: The culture medium was removed and then the cells were washed with PBS and then incubated for 30 min at 37 ℃ with MitoS OX (for measurement of mROS superoxide; Invitrogen) and/or CM-H2DCFDA or CellROX (for measurement of total cellular H2 O2 ; Invitrogen) at a final concentration of 5 µM in serum-free DMEM (Invitrogen).

    Techniques: Flow Cytometry, Cytometry, Infection, Expressing, Fluorescence, Quantitative RT-PCR, Microscopy, Staining, Fluorescence In Situ Hybridization, Isolation, Mouse Assay, Transduction

    Mst1/2 sense phagosomal and mitochondrial ROS. a Flow cytometry analysis of cellular ROS levels in WT BMDMs pretreated with or without NAC, infected with E. coli (MOI: 100) and stained with CellRox for 30 min. b SIM of Mst1 staining (red) and DAPI-stained nuclei (blue) in WT BMDMs infected with GFP- E. coli (green) treated with or without NAC as indicated; ×25 magnification of areas outlined in the main images are shown next to the main images. Scale bars, 20 μm. c Immunoblot analysis of phosphorylated (p)-Mob1, Mob1, p-Mst1/2, Mst1, Mst2, and GAPDH in BMDMs pretreated with PBS or NAC (5 μM) and then infected with E. coli (MOI: 100). d Immunoblot analysis of Mst1, Mst2, β-actin and Hsp60 in the cytoplasmic (Cyto) and mitochondrial (Mito) fractions of NAC-treated or non-treated BMDMs infected with E. coli (MOI: 100) for the indicated time. e SIM of Mst1 staining (red), Tomm20 (green) and DAPI-stained nuclei (blue) in WT BMDMs treated with DMSO or antimycin A, with or without NAC pretreatment, as indicated; ×49 magnification of areas outlined in the main images are shown next to the main images. Scale bars, 20 μm. f , g Immunoblot analysis of Mst1, Mst2, β-actin, and Hsp60 in the cytoplasmic (Cyto) and mitochondrial (Mito) fractions of WT BMDMs treated with antimycin A ( f ) or rotenone ( g ), with or without NAC pretreatment, for the indicated time. h , i Immunoblot analysis of p-Mob1, Mob1, p-Mst1/2, Mst1, Mst2, and GAPDH in BMDMs treated with antimycin A ( h ) or rotenone ( i ) for the indicated time or with antimycin A ( h ) or rotenone ( i ) at the indicated dose for 30 min. j , k Immunoblot analysis of p-Mob1, Mob1, p-Mst1/2, Mst1, Mst2, and GAPDH in PBS or NAC pretreated BMDMs followed with 10 μM antimycin A ( j ) or 3 μM rotenone ( k ) treatment. l Flow cytometry analysis of mitochondrial ROS levels in THP1 cells treated with 10 μM antimycin A or 3 μM rotenone followed with or without Mst1/2 kinases inhibitor, XMU-MP-1, treatment for indicated times. Data are from one experiment representative of three independent experiments with similar results

    Journal: Nature Communications

    Article Title: Macrophage achieves self-protection against oxidative stress-induced ageing through the Mst-Nrf2 axis

    doi: 10.1038/s41467-019-08680-6

    Figure Lengend Snippet: Mst1/2 sense phagosomal and mitochondrial ROS. a Flow cytometry analysis of cellular ROS levels in WT BMDMs pretreated with or without NAC, infected with E. coli (MOI: 100) and stained with CellRox for 30 min. b SIM of Mst1 staining (red) and DAPI-stained nuclei (blue) in WT BMDMs infected with GFP- E. coli (green) treated with or without NAC as indicated; ×25 magnification of areas outlined in the main images are shown next to the main images. Scale bars, 20 μm. c Immunoblot analysis of phosphorylated (p)-Mob1, Mob1, p-Mst1/2, Mst1, Mst2, and GAPDH in BMDMs pretreated with PBS or NAC (5 μM) and then infected with E. coli (MOI: 100). d Immunoblot analysis of Mst1, Mst2, β-actin and Hsp60 in the cytoplasmic (Cyto) and mitochondrial (Mito) fractions of NAC-treated or non-treated BMDMs infected with E. coli (MOI: 100) for the indicated time. e SIM of Mst1 staining (red), Tomm20 (green) and DAPI-stained nuclei (blue) in WT BMDMs treated with DMSO or antimycin A, with or without NAC pretreatment, as indicated; ×49 magnification of areas outlined in the main images are shown next to the main images. Scale bars, 20 μm. f , g Immunoblot analysis of Mst1, Mst2, β-actin, and Hsp60 in the cytoplasmic (Cyto) and mitochondrial (Mito) fractions of WT BMDMs treated with antimycin A ( f ) or rotenone ( g ), with or without NAC pretreatment, for the indicated time. h , i Immunoblot analysis of p-Mob1, Mob1, p-Mst1/2, Mst1, Mst2, and GAPDH in BMDMs treated with antimycin A ( h ) or rotenone ( i ) for the indicated time or with antimycin A ( h ) or rotenone ( i ) at the indicated dose for 30 min. j , k Immunoblot analysis of p-Mob1, Mob1, p-Mst1/2, Mst1, Mst2, and GAPDH in PBS or NAC pretreated BMDMs followed with 10 μM antimycin A ( j ) or 3 μM rotenone ( k ) treatment. l Flow cytometry analysis of mitochondrial ROS levels in THP1 cells treated with 10 μM antimycin A or 3 μM rotenone followed with or without Mst1/2 kinases inhibitor, XMU-MP-1, treatment for indicated times. Data are from one experiment representative of three independent experiments with similar results

    Article Snippet: The culture medium was removed and then the cells were washed with PBS and then incubated for 30 min at 37 ℃ with MitoS OX (for measurement of mROS superoxide; Invitrogen) and/or CM-H2DCFDA or CellROX (for measurement of total cellular H2 O2 ; Invitrogen) at a final concentration of 5 µM in serum-free DMEM (Invitrogen).

    Techniques: Flow Cytometry, Cytometry, Infection, Staining

    Genes identified from a positive-selection CRISPR/Cas9 screen using H2 O2 .

    Journal: mBio

    Article Title: A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells

    doi: 10.1128/mBio.01704-20

    Figure Lengend Snippet: Genes identified from a positive-selection CRISPR/Cas9 screen using H2 O2 .

    Article Snippet: 10.1128/mBio.01704-20.1 FIG S1 Cytotoxicity curve for H2 O2 in WT HAP1 cells.

    Techniques: Selection, CRISPR