Structured Review

Millipore h2 o2
H2 O2, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2 o2/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
h2 o2 - by Bioz Stars, 2021-03
86/100 stars

Images

Related Articles

Incubation:

Article Title: Early neuronal accumulation of DNA double strand breaks in Alzheimer’s disease
Article Snippet: Briefly, sections were washed in Tris-buffered saline (TBS), permeabilized in TBS containing 0.5% Triton X-100 (Sigma-Aldrich) at 4 °C overnight, and incubated for 15 min at 110 °C in citrate buffer (pH 6.0) for antigen retrieval. .. They were then incubated in 3% H2 O2 and 10% methanol for 15 min, 0.1% Sudan Black (Sigma-Aldrich) in 70% ethanol for 15 min, and 10% goat serum in TBS containing 0.1% Tween-20 (TBST) for 1 h. Anti-γH2AX and anti-NeuN antibodies (Additional file : Table S3) in TBST containing 3% goat serum were applied first at 4 °C overnight and then at room temperature for 2 h. Primary antibodies were detected with Alexa Fluor secondary antibodies (Thermo Fisher Scientific) at 1:500 dilution. ..

Staining:

Article Title: Penfluridol triggers cytoprotective autophagy and cellular apoptosis through ROS induction and activation of the PP2A-modulated MAPK pathway in acute myeloid leukemia with different FLT3 statuses
Article Snippet: Densitometric analysis of the bands was carried out using Image-Pro Plus software (Media Cybernetics, Rockville, MD). .. Measurement of reactive oxygen species (ROS) production AML cells were treated with penfluridol or H2 O2 for the indicated time points, and the intracellular ROS production was detected by staining with the fluoroprobe, 2′,7′-dichlorofluorescin diacetate (DCFDA, Sigma), in RPMI 1640 medium for 30 min. After washing cells with PBS, ROS production of DCFDA-preloaded cells was captured using a fluorescence microscope (Zeiss Axioplan) or quantified by flow cytometry. .. Detection and quantification of autophagic cells by staining with acridine orange (AO) Formation of acidic vesicular organelles (AVOs), a morphological characteristic of autophagy, was detected by AO (Sigma) staining.

Fluorescence:

Article Title: Penfluridol triggers cytoprotective autophagy and cellular apoptosis through ROS induction and activation of the PP2A-modulated MAPK pathway in acute myeloid leukemia with different FLT3 statuses
Article Snippet: Densitometric analysis of the bands was carried out using Image-Pro Plus software (Media Cybernetics, Rockville, MD). .. Measurement of reactive oxygen species (ROS) production AML cells were treated with penfluridol or H2 O2 for the indicated time points, and the intracellular ROS production was detected by staining with the fluoroprobe, 2′,7′-dichlorofluorescin diacetate (DCFDA, Sigma), in RPMI 1640 medium for 30 min. After washing cells with PBS, ROS production of DCFDA-preloaded cells was captured using a fluorescence microscope (Zeiss Axioplan) or quantified by flow cytometry. .. Detection and quantification of autophagic cells by staining with acridine orange (AO) Formation of acidic vesicular organelles (AVOs), a morphological characteristic of autophagy, was detected by AO (Sigma) staining.

Microscopy:

Article Title: Penfluridol triggers cytoprotective autophagy and cellular apoptosis through ROS induction and activation of the PP2A-modulated MAPK pathway in acute myeloid leukemia with different FLT3 statuses
Article Snippet: Densitometric analysis of the bands was carried out using Image-Pro Plus software (Media Cybernetics, Rockville, MD). .. Measurement of reactive oxygen species (ROS) production AML cells were treated with penfluridol or H2 O2 for the indicated time points, and the intracellular ROS production was detected by staining with the fluoroprobe, 2′,7′-dichlorofluorescin diacetate (DCFDA, Sigma), in RPMI 1640 medium for 30 min. After washing cells with PBS, ROS production of DCFDA-preloaded cells was captured using a fluorescence microscope (Zeiss Axioplan) or quantified by flow cytometry. .. Detection and quantification of autophagic cells by staining with acridine orange (AO) Formation of acidic vesicular organelles (AVOs), a morphological characteristic of autophagy, was detected by AO (Sigma) staining.

Flow Cytometry:

Article Title: Penfluridol triggers cytoprotective autophagy and cellular apoptosis through ROS induction and activation of the PP2A-modulated MAPK pathway in acute myeloid leukemia with different FLT3 statuses
Article Snippet: Densitometric analysis of the bands was carried out using Image-Pro Plus software (Media Cybernetics, Rockville, MD). .. Measurement of reactive oxygen species (ROS) production AML cells were treated with penfluridol or H2 O2 for the indicated time points, and the intracellular ROS production was detected by staining with the fluoroprobe, 2′,7′-dichlorofluorescin diacetate (DCFDA, Sigma), in RPMI 1640 medium for 30 min. After washing cells with PBS, ROS production of DCFDA-preloaded cells was captured using a fluorescence microscope (Zeiss Axioplan) or quantified by flow cytometry. .. Detection and quantification of autophagic cells by staining with acridine orange (AO) Formation of acidic vesicular organelles (AVOs), a morphological characteristic of autophagy, was detected by AO (Sigma) staining.

Cytometry:

Article Title: Penfluridol triggers cytoprotective autophagy and cellular apoptosis through ROS induction and activation of the PP2A-modulated MAPK pathway in acute myeloid leukemia with different FLT3 statuses
Article Snippet: Densitometric analysis of the bands was carried out using Image-Pro Plus software (Media Cybernetics, Rockville, MD). .. Measurement of reactive oxygen species (ROS) production AML cells were treated with penfluridol or H2 O2 for the indicated time points, and the intracellular ROS production was detected by staining with the fluoroprobe, 2′,7′-dichlorofluorescin diacetate (DCFDA, Sigma), in RPMI 1640 medium for 30 min. After washing cells with PBS, ROS production of DCFDA-preloaded cells was captured using a fluorescence microscope (Zeiss Axioplan) or quantified by flow cytometry. .. Detection and quantification of autophagic cells by staining with acridine orange (AO) Formation of acidic vesicular organelles (AVOs), a morphological characteristic of autophagy, was detected by AO (Sigma) staining.

MTT Assay:

Article Title: Exendin-4 Pretreated Adipose Derived Stem Cells Are Resistant to Oxidative Stress and Improve Cardiac Performance via Enhanced Adhesion in the Infarcted Heart
Article Snippet: Cell proliferation assay ADSCs were plated in a 96-well plate at a density of 1×104 /well. .. After pretreatment with 50 nM Exendin-4, H2 O2 was given to the cells for 6 h, a total of 5 g/L 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) in medium was added to the cells for 2 h. Then the medium was removed and dimethyl sulphoxide (DMSO) was applied at a volume of 200 µL/well. .. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) ADSCs that were treated by H2 O2 with or without Exendin-4 were collected.

Activity Assay:

Article Title: Hybrid caffeic acid derivatives as monoamine oxidases inhibitors: synthesis, radical scavenging activity, molecular docking studies and in silico ADMET analysis
Article Snippet: Materials and methods Unless otherwise noted, the chemicals required for synthesis and antioxidant activity were purchased from Hi-media Laboratories. .. The biological hMAO activity evaluation of the test drugs was examined by quantifying their action on the generation of H2 O2 by p-tyramine (general substrate for hMAO-B and hMAO-A), utilizing the Amplex Red MAO assay kit (Sigma USA) and MAO isoforms (microsomal) obtained from insect cells (BTI-TN-5B14) expressed as recombinant baculovirus consisting cDNA probes for hMAO-A or hMAO-B. .. Reactions were monitored by thin layer chromatography TLC executed over silica gel precoated plates (0.25 mm) purchased from Merck, envisioned of single spots was carried in iodine and UV chambers, in mobile media TLC- Benzene:Chloroform (7:3).

Recombinant:

Article Title: Hybrid caffeic acid derivatives as monoamine oxidases inhibitors: synthesis, radical scavenging activity, molecular docking studies and in silico ADMET analysis
Article Snippet: Materials and methods Unless otherwise noted, the chemicals required for synthesis and antioxidant activity were purchased from Hi-media Laboratories. .. The biological hMAO activity evaluation of the test drugs was examined by quantifying their action on the generation of H2 O2 by p-tyramine (general substrate for hMAO-B and hMAO-A), utilizing the Amplex Red MAO assay kit (Sigma USA) and MAO isoforms (microsomal) obtained from insect cells (BTI-TN-5B14) expressed as recombinant baculovirus consisting cDNA probes for hMAO-A or hMAO-B. .. Reactions were monitored by thin layer chromatography TLC executed over silica gel precoated plates (0.25 mm) purchased from Merck, envisioned of single spots was carried in iodine and UV chambers, in mobile media TLC- Benzene:Chloroform (7:3).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Millipore peg catalase
    VL are enriched in and functionally dependent on NADPH oxidase activity. (a) MVECs coexpressing <t>p47phox-DsRed</t> and actin-GFP were imaged live during T cell diapedesis. p47phox was enriched in ventral (IRM) actin-rich initiation nodes (N) and in VL leading edge (arrowheads). See Video 10 . (b) Representative ratiometric images of H 2 O 2 production in (i) pore- and (ii) gap-closing VL in MVECs expressing the biosensor mHyPer during T cell diapedesis. (c) Quantification of p47phox (i) and H 2 O 2 (ii) enrichment in VL. n > 13. (d, i) Representative NADPH oxidase inhibition/washout imaging experiment. After multiple T cell diapedesis events on MVECs expressing mYFP, apocynin was added. Arrows indicate multiple pores and gaps that persisted for 15 min, but were rapidly healed by steered VL (arrowheads) after drug washout. See Video 10. Experiments as in d (i) were performed with VAS-2870, DPI, <t>PEG-catalase,</t> and DMSO (control) and quantified by cumulative wound closure analysis, shown as a continuous line plot (ii) and bar graph of single pre/post-washout time points (iii). n > 5. (e) MVECs coexpressing mDsRed and mHyPer were imaged during probe wounds and after addition of VAS-2870. Representative mDsRed images (i), kymograph (ii), mHyper ratiometic images (iii), and quantitation (iv) are shown. n = 3. Values represent mean ± SEM. Statistical significance is indicated with p-values as follows: ***, P
    Peg Catalase, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peg catalase/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peg catalase - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    93
    Millipore dimethylthiourea
    Effects of H 2 O 2 and ethylene inhibitors on BRs-induced AOX capacity in response to environmental stress . (A–D) Plants were pretreated with 100 μM DPI, 5 mM <t>DMTU</t> for 8 h or 1 mM AOA for 12 h, then plants were treated with 1 μM BL 12 h later, plants were challenged with stress conditions for 3 d. Data are the mean ± SD of three biological repeats; the significant difference was analyzed by Student's t -test ( * P
    Dimethylthiourea, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dimethylthiourea/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dimethylthiourea - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    93
    Millipore dmtu
    Effects of salicylic acid (SA), H 2 O 2 , imidazole (IMD), catalase <t>(CAT),</t> dimethylthiourea <t>(DMTU)</t> and complex treatments on the production of Sal B and H 2 O 2 in Salvia miltiorrhiza cell cultures. The concentrations for SA, H 2 O 2 , IMD, CAT and DMTU were 22 mg·L − 1 , 10 mmol·L − 1 , 100 μmol·L − 1 , 100 U and 500 μmol·L − 1 , respectively. SA was added to subcultured Salvia miltiorrhiza cells after 8 d culture, and both contents of H 2 O 2 and Sal B were determined in the subsequent 8 h and 2 d, respectively, with three replications. Different lowercase letters represented significance at 0.05.
    Dmtu, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmtu/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmtu - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    VL are enriched in and functionally dependent on NADPH oxidase activity. (a) MVECs coexpressing p47phox-DsRed and actin-GFP were imaged live during T cell diapedesis. p47phox was enriched in ventral (IRM) actin-rich initiation nodes (N) and in VL leading edge (arrowheads). See Video 10 . (b) Representative ratiometric images of H 2 O 2 production in (i) pore- and (ii) gap-closing VL in MVECs expressing the biosensor mHyPer during T cell diapedesis. (c) Quantification of p47phox (i) and H 2 O 2 (ii) enrichment in VL. n > 13. (d, i) Representative NADPH oxidase inhibition/washout imaging experiment. After multiple T cell diapedesis events on MVECs expressing mYFP, apocynin was added. Arrows indicate multiple pores and gaps that persisted for 15 min, but were rapidly healed by steered VL (arrowheads) after drug washout. See Video 10. Experiments as in d (i) were performed with VAS-2870, DPI, PEG-catalase, and DMSO (control) and quantified by cumulative wound closure analysis, shown as a continuous line plot (ii) and bar graph of single pre/post-washout time points (iii). n > 5. (e) MVECs coexpressing mDsRed and mHyPer were imaged during probe wounds and after addition of VAS-2870. Representative mDsRed images (i), kymograph (ii), mHyper ratiometic images (iii), and quantitation (iv) are shown. n = 3. Values represent mean ± SEM. Statistical significance is indicated with p-values as follows: ***, P

    Journal: The Journal of Cell Biology

    Article Title: Release of cellular tension signals self-restorative ventral lamellipodia to heal barrier micro-wounds

    doi: 10.1083/jcb.201209077

    Figure Lengend Snippet: VL are enriched in and functionally dependent on NADPH oxidase activity. (a) MVECs coexpressing p47phox-DsRed and actin-GFP were imaged live during T cell diapedesis. p47phox was enriched in ventral (IRM) actin-rich initiation nodes (N) and in VL leading edge (arrowheads). See Video 10 . (b) Representative ratiometric images of H 2 O 2 production in (i) pore- and (ii) gap-closing VL in MVECs expressing the biosensor mHyPer during T cell diapedesis. (c) Quantification of p47phox (i) and H 2 O 2 (ii) enrichment in VL. n > 13. (d, i) Representative NADPH oxidase inhibition/washout imaging experiment. After multiple T cell diapedesis events on MVECs expressing mYFP, apocynin was added. Arrows indicate multiple pores and gaps that persisted for 15 min, but were rapidly healed by steered VL (arrowheads) after drug washout. See Video 10. Experiments as in d (i) were performed with VAS-2870, DPI, PEG-catalase, and DMSO (control) and quantified by cumulative wound closure analysis, shown as a continuous line plot (ii) and bar graph of single pre/post-washout time points (iii). n > 5. (e) MVECs coexpressing mDsRed and mHyPer were imaged during probe wounds and after addition of VAS-2870. Representative mDsRed images (i), kymograph (ii), mHyper ratiometic images (iii), and quantitation (iv) are shown. n = 3. Values represent mean ± SEM. Statistical significance is indicated with p-values as follows: ***, P

    Article Snippet: Inhibitors of NADPH oxidase signaling used included VAS-2870 (Nox2/4 inhibitor; 15 µM; Enzo Life Sciences), DPI (non-specific Nox inhibitor/Flavoprotein inhibitor; 12.5 µM; Sigma-Aldrich), apocynin (inhibitor of p47phox assembly into Nox complex/H2 O2 scavenger; 1 mM; Sigma-Aldrich), PEG-catalase (H2 O2 degrading enzyme; 200 U/ml; Sigma-Aldrich), and Tempol (superoxide dismutase/catalase mimetic; 500 µM; Sigma-Aldrich).

    Techniques: Activity Assay, Expressing, Inhibition, Imaging, Quantitation Assay

    Effects of H 2 O 2 and ethylene inhibitors on BRs-induced AOX capacity in response to environmental stress . (A–D) Plants were pretreated with 100 μM DPI, 5 mM DMTU for 8 h or 1 mM AOA for 12 h, then plants were treated with 1 μM BL 12 h later, plants were challenged with stress conditions for 3 d. Data are the mean ± SD of three biological repeats; the significant difference was analyzed by Student's t -test ( * P

    Journal: Frontiers in Plant Science

    Article Title: Ethylene is Involved in Brassinosteroids Induced Alternative Respiratory Pathway in Cucumber (Cucumis sativus L.) Seedlings Response to Abiotic Stress

    doi: 10.3389/fpls.2015.00982

    Figure Lengend Snippet: Effects of H 2 O 2 and ethylene inhibitors on BRs-induced AOX capacity in response to environmental stress . (A–D) Plants were pretreated with 100 μM DPI, 5 mM DMTU for 8 h or 1 mM AOA for 12 h, then plants were treated with 1 μM BL 12 h later, plants were challenged with stress conditions for 3 d. Data are the mean ± SD of three biological repeats; the significant difference was analyzed by Student's t -test ( * P

    Article Snippet: Treatment Salicylhydroxamic acid (SHAM, an inhibitor of the AOX pathway), dimethylthiourea (DMTU, an H2 O2 scavenger), DPI (an NADPH oxidase inhibitor), aminooxyacetic acid (AOA, an ethylene biosynthesis inhibitor), and ethylene were purchased from Sigma (StLouis, USA).

    Techniques:

    Changes of ethylene emission ( A ), ACC content ( B ), ACS activity ( C ) and ACO activity ( D ) in H 2 O 2 scavenger DMTU or inhibitor DPI pre-treated plants as influenced by 0.1 μM BL. Tomato plants were treated with 5 mM DMTU for 8 h and then treated with 0.1 μM BL for another 12 h. Single treatment of BL or DMTU/DPI was included as control.Changes in ethylene emission ( E ) ACC content ( F ), ACS activity ( G ) and ACO activity ( H ) in SlBRI1 -, SlBAK1 - and SlDWARF -silenced plants as influenced by 0.1 μM BL. Involvement of H 2 O 2 in the BR-induced ethylene accumulation. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P

    Journal: Scientific Reports

    Article Title: Ethylene and hydrogen peroxide are involved in brassinosteroid-induced salt tolerance in tomato

    doi: 10.1038/srep35392

    Figure Lengend Snippet: Changes of ethylene emission ( A ), ACC content ( B ), ACS activity ( C ) and ACO activity ( D ) in H 2 O 2 scavenger DMTU or inhibitor DPI pre-treated plants as influenced by 0.1 μM BL. Tomato plants were treated with 5 mM DMTU for 8 h and then treated with 0.1 μM BL for another 12 h. Single treatment of BL or DMTU/DPI was included as control.Changes in ethylene emission ( E ) ACC content ( F ), ACS activity ( G ) and ACO activity ( H ) in SlBRI1 -, SlBAK1 - and SlDWARF -silenced plants as influenced by 0.1 μM BL. Involvement of H 2 O 2 in the BR-induced ethylene accumulation. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P

    Article Snippet: Dimethylthiourea (DMTU, an H2 O2 scavenger), 1-methylcyclopropene (1-MCP) and diphenylene iodonium (DPI, an NADPH oxidase inhibitor) were purchased from Sigma (St Louis, USA).

    Techniques: Activity Assay, Standard Deviation

    Effects of salicylic acid (SA), H 2 O 2 , imidazole (IMD), catalase (CAT), dimethylthiourea (DMTU) and complex treatments on the production of Sal B and H 2 O 2 in Salvia miltiorrhiza cell cultures. The concentrations for SA, H 2 O 2 , IMD, CAT and DMTU were 22 mg·L − 1 , 10 mmol·L − 1 , 100 μmol·L − 1 , 100 U and 500 μmol·L − 1 , respectively. SA was added to subcultured Salvia miltiorrhiza cells after 8 d culture, and both contents of H 2 O 2 and Sal B were determined in the subsequent 8 h and 2 d, respectively, with three replications. Different lowercase letters represented significance at 0.05.

    Journal: Molecules

    Article Title: Hydrogen Peroxide and Nitric Oxide are Involved in Salicylic Acid-Induced Salvianolic Acid B Production in Salvia miltiorrhiza Cell Cultures

    doi: 10.3390/molecules19055913

    Figure Lengend Snippet: Effects of salicylic acid (SA), H 2 O 2 , imidazole (IMD), catalase (CAT), dimethylthiourea (DMTU) and complex treatments on the production of Sal B and H 2 O 2 in Salvia miltiorrhiza cell cultures. The concentrations for SA, H 2 O 2 , IMD, CAT and DMTU were 22 mg·L − 1 , 10 mmol·L − 1 , 100 μmol·L − 1 , 100 U and 500 μmol·L − 1 , respectively. SA was added to subcultured Salvia miltiorrhiza cells after 8 d culture, and both contents of H 2 O 2 and Sal B were determined in the subsequent 8 h and 2 d, respectively, with three replications. Different lowercase letters represented significance at 0.05.

    Article Snippet: Stock solutions of H2 O2 , DMTU (H2 O2 scavenger, Sigma, Shanghai, China), CAT (Sigma), IMD (NADPH scavenger, Sigma), sodium nitroprusside (SNP, NO donor, Sigma), Nω -nitro-L-arginine (L-NNA, inhibitor of NO synthase, Sigma) and cPTIO (NO scavenger, Sigma) were prepared in distilled water and then sterilized after filtration through 0.22 μm membrane.

    Techniques: Radial Immuno Diffusion

    Effects of SA, exogenous H 2 O 2 , IMD, DMTU and complex treatments on the production of Sal B and NO in Salvia miltiorrhiza cell cultures. The concentrations for SA, H 2 O 2 , IMD, CAT and DMTU were the same as Figure 1 . The detection time for determining both contents of NO and Sal B was the same as Figure 3 . Different lowercase letters represented significance at 0.05.

    Journal: Molecules

    Article Title: Hydrogen Peroxide and Nitric Oxide are Involved in Salicylic Acid-Induced Salvianolic Acid B Production in Salvia miltiorrhiza Cell Cultures

    doi: 10.3390/molecules19055913

    Figure Lengend Snippet: Effects of SA, exogenous H 2 O 2 , IMD, DMTU and complex treatments on the production of Sal B and NO in Salvia miltiorrhiza cell cultures. The concentrations for SA, H 2 O 2 , IMD, CAT and DMTU were the same as Figure 1 . The detection time for determining both contents of NO and Sal B was the same as Figure 3 . Different lowercase letters represented significance at 0.05.

    Article Snippet: Stock solutions of H2 O2 , DMTU (H2 O2 scavenger, Sigma, Shanghai, China), CAT (Sigma), IMD (NADPH scavenger, Sigma), sodium nitroprusside (SNP, NO donor, Sigma), Nω -nitro-L-arginine (L-NNA, inhibitor of NO synthase, Sigma) and cPTIO (NO scavenger, Sigma) were prepared in distilled water and then sterilized after filtration through 0.22 μm membrane.

    Techniques: Radial Immuno Diffusion