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Becton Dickinson h2 o2
H2 O2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Isolation:

Article Title: Modulatory effects of low-dose hydrogen peroxide on the function of human plasmacytoid dendritic cells
Article Snippet: Fluorescence intensities were measured by a FACSCalibur flow cytometer (BD Biosciences Immunocytometry Systems, Franklin Lakes, NJ, USA) and analysis of data was performed by FlowJo software (TreeStar, Ashland, OR, USA). .. Freshly isolated untouched pDCs and conventional DCs were loaded with 50 µM 2′,7′-dihydrodichlorofluorescein diacetate (H2 DCFDA; Invitrogen) at 37°C for 20 min. After excess fluorescent dye was removed, the cells were exposed to increasing concentrations of H2 O2 for 2 h. Changes in DCF fluorescence intensity were detected on the FL1 (530±15 nm) channel using a BD FACSCalibur flow cytometer. ..

Fluorescence:

Article Title: Modulatory effects of low-dose hydrogen peroxide on the function of human plasmacytoid dendritic cells
Article Snippet: Fluorescence intensities were measured by a FACSCalibur flow cytometer (BD Biosciences Immunocytometry Systems, Franklin Lakes, NJ, USA) and analysis of data was performed by FlowJo software (TreeStar, Ashland, OR, USA). .. Freshly isolated untouched pDCs and conventional DCs were loaded with 50 µM 2′,7′-dihydrodichlorofluorescein diacetate (H2 DCFDA; Invitrogen) at 37°C for 20 min. After excess fluorescent dye was removed, the cells were exposed to increasing concentrations of H2 O2 for 2 h. Changes in DCF fluorescence intensity were detected on the FL1 (530±15 nm) channel using a BD FACSCalibur flow cytometer. ..

Flow Cytometry:

Article Title: Modulatory effects of low-dose hydrogen peroxide on the function of human plasmacytoid dendritic cells
Article Snippet: Fluorescence intensities were measured by a FACSCalibur flow cytometer (BD Biosciences Immunocytometry Systems, Franklin Lakes, NJ, USA) and analysis of data was performed by FlowJo software (TreeStar, Ashland, OR, USA). .. Freshly isolated untouched pDCs and conventional DCs were loaded with 50 µM 2′,7′-dihydrodichlorofluorescein diacetate (H2 DCFDA; Invitrogen) at 37°C for 20 min. After excess fluorescent dye was removed, the cells were exposed to increasing concentrations of H2 O2 for 2 h. Changes in DCF fluorescence intensity were detected on the FL1 (530±15 nm) channel using a BD FACSCalibur flow cytometer. ..

Article Title: Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways
Article Snippet: .. Then the cells pre-incubated with drugs for 12 h or 24 h were subsequently treated with 100 μM H2 O2 for 12 h. Cells were analyzed by FACScan flow cytometer (Becton Dickinson) with FlowJo software (Tree Star Inc.) and down regulation effect of plasmid DNA on PC12 cells gene was evaluated by western blot. ..

Cytometry:

Article Title: Modulatory effects of low-dose hydrogen peroxide on the function of human plasmacytoid dendritic cells
Article Snippet: Fluorescence intensities were measured by a FACSCalibur flow cytometer (BD Biosciences Immunocytometry Systems, Franklin Lakes, NJ, USA) and analysis of data was performed by FlowJo software (TreeStar, Ashland, OR, USA). .. Freshly isolated untouched pDCs and conventional DCs were loaded with 50 µM 2′,7′-dihydrodichlorofluorescein diacetate (H2 DCFDA; Invitrogen) at 37°C for 20 min. After excess fluorescent dye was removed, the cells were exposed to increasing concentrations of H2 O2 for 2 h. Changes in DCF fluorescence intensity were detected on the FL1 (530±15 nm) channel using a BD FACSCalibur flow cytometer. ..

Article Title: Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways
Article Snippet: .. Then the cells pre-incubated with drugs for 12 h or 24 h were subsequently treated with 100 μM H2 O2 for 12 h. Cells were analyzed by FACScan flow cytometer (Becton Dickinson) with FlowJo software (Tree Star Inc.) and down regulation effect of plasmid DNA on PC12 cells gene was evaluated by western blot. ..

Multiple Displacement Amplification:

Article Title: Effects of Glutamate and Aspartate on Serum Antioxidative Enzyme, Sex Hormones, and Genital Inflammation in Boars Challenged with Hydrogen Peroxide
Article Snippet: .. In this study, we found that aspartate was capable of reducing the MDA level in boar serum, while glutamate failed to alleviate H2 O2 -induced oxidative stress in boars. .. Boars in the BD group even had higher serum GSH-Px level than those in the GLU group.

Software:

Article Title: Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways
Article Snippet: .. Then the cells pre-incubated with drugs for 12 h or 24 h were subsequently treated with 100 μM H2 O2 for 12 h. Cells were analyzed by FACScan flow cytometer (Becton Dickinson) with FlowJo software (Tree Star Inc.) and down regulation effect of plasmid DNA on PC12 cells gene was evaluated by western blot. ..

Plasmid Preparation:

Article Title: Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways
Article Snippet: .. Then the cells pre-incubated with drugs for 12 h or 24 h were subsequently treated with 100 μM H2 O2 for 12 h. Cells were analyzed by FACScan flow cytometer (Becton Dickinson) with FlowJo software (Tree Star Inc.) and down regulation effect of plasmid DNA on PC12 cells gene was evaluated by western blot. ..

Western Blot:

Article Title: Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways
Article Snippet: .. Then the cells pre-incubated with drugs for 12 h or 24 h were subsequently treated with 100 μM H2 O2 for 12 h. Cells were analyzed by FACScan flow cytometer (Becton Dickinson) with FlowJo software (Tree Star Inc.) and down regulation effect of plasmid DNA on PC12 cells gene was evaluated by western blot. ..

Incubation:

Article Title: Gastrointestinal Infection with Mexican TcI Trypanosoma cruzi strains: Different Degrees of Colonization and Diverse Immune Responses
Article Snippet: .. The sections were fixed with cold acetone and washed three times with PBS, followed by incubation with 0.3% H2 O2 ; after three additional washes with PBS, the sections were incubated with 2% BSA for 1 h. Anti-CD4+ (BD Pharmigen, Bedford, MA, USA) and anti-CD8+ antibodies (AbD serotec, Raleigh, NC, USA), or isotype controls (AbD serotec), were added and allowed to incubate overnight at 4°C. .. After three washes with PBS, biotin-conjugated goat anti-rat IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was added for 90 min at room temperature.

Article Title: Stromal interaction essential for vascular endothelial growth factor A-induced tumour growth via transforming growth factor-β signalling
Article Snippet: Immunohistochemical analysis for vessel density and proliferation Frozen tumour samples were cut into 4-μ m sections and studied for vessel density by staining with CD31 (platelet endothelial cell adhesion molecule (PECAM)-1) as well as for proliferation by staining with Ki67. .. Sections were blocked for endogene peroxidase with 0.25% H2 O2 and incubated with mouse-specific CD31 (BD). .. Subsequently, sections were incubated with secondary antibody (swine) anti-rat biotin (DAKO), amplified with (biotin) streptavidin ABComplex/HRPO (DAKO) and detected by 3-amino-9-ethylcarbazole (Sigma).

Article Title: Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex
Article Snippet: For double staining with Cux1 and Ctip2, antigen retrieval was performed by heating the sections at more than 95 °C for 20 min in 10 mM citrate buffer (pH 6.0). .. For BrdU staining after antigen retrieval, the sections were incubated with 2N HCl at 37 °C for 15 min and neutralized by immersing in borate buffer (0.1 M, pH 8.5) for 20 min. After blocking endogenous peroxidase with 3% H2 O2 in methanol for 30 min, sections were incubated in biotinylated anti-BrdU antibody for 1 h, followed by treatment with a streptavidin–HRP (BD Pharmingen BrdU in situ detection kit, BD Biosciences). .. AntibodiesRabbit polyclonal antibodies against SAD-A peptide (aa 441–455) were prepared and purified, and detected only SAD-A protein but not SAD-B protein. ( ).

BrdU Staining:

Article Title: Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex
Article Snippet: For double staining with Cux1 and Ctip2, antigen retrieval was performed by heating the sections at more than 95 °C for 20 min in 10 mM citrate buffer (pH 6.0). .. For BrdU staining after antigen retrieval, the sections were incubated with 2N HCl at 37 °C for 15 min and neutralized by immersing in borate buffer (0.1 M, pH 8.5) for 20 min. After blocking endogenous peroxidase with 3% H2 O2 in methanol for 30 min, sections were incubated in biotinylated anti-BrdU antibody for 1 h, followed by treatment with a streptavidin–HRP (BD Pharmingen BrdU in situ detection kit, BD Biosciences). .. AntibodiesRabbit polyclonal antibodies against SAD-A peptide (aa 441–455) were prepared and purified, and detected only SAD-A protein but not SAD-B protein. ( ).

Blocking Assay:

Article Title: Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex
Article Snippet: For double staining with Cux1 and Ctip2, antigen retrieval was performed by heating the sections at more than 95 °C for 20 min in 10 mM citrate buffer (pH 6.0). .. For BrdU staining after antigen retrieval, the sections were incubated with 2N HCl at 37 °C for 15 min and neutralized by immersing in borate buffer (0.1 M, pH 8.5) for 20 min. After blocking endogenous peroxidase with 3% H2 O2 in methanol for 30 min, sections were incubated in biotinylated anti-BrdU antibody for 1 h, followed by treatment with a streptavidin–HRP (BD Pharmingen BrdU in situ detection kit, BD Biosciences). .. AntibodiesRabbit polyclonal antibodies against SAD-A peptide (aa 441–455) were prepared and purified, and detected only SAD-A protein but not SAD-B protein. ( ).

In Situ:

Article Title: Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex
Article Snippet: For double staining with Cux1 and Ctip2, antigen retrieval was performed by heating the sections at more than 95 °C for 20 min in 10 mM citrate buffer (pH 6.0). .. For BrdU staining after antigen retrieval, the sections were incubated with 2N HCl at 37 °C for 15 min and neutralized by immersing in borate buffer (0.1 M, pH 8.5) for 20 min. After blocking endogenous peroxidase with 3% H2 O2 in methanol for 30 min, sections were incubated in biotinylated anti-BrdU antibody for 1 h, followed by treatment with a streptavidin–HRP (BD Pharmingen BrdU in situ detection kit, BD Biosciences). .. AntibodiesRabbit polyclonal antibodies against SAD-A peptide (aa 441–455) were prepared and purified, and detected only SAD-A protein but not SAD-B protein. ( ).

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  • 86
    Becton Dickinson tmb substrate reagent set
    Binding of the SWNT antibody complex to its specific target. ( A ) Typical saturation curve. ( B ) Lineweaver–Burk plots. The data derived from the saturation curve were fitted to the dashed line (see text). The line represents 1/V max (obtained from ordinate intercept) and 1/K m (obtained from abscissa intercept). 1/ v = 0.007373 (1/[SWNT]) + 0.000812, R 2 = 0.999. V max,SWNT = 1231, K m,SWNT = 9.09 (μg/mL). ( C ) Hill plots. Slope of the line represents n H = 0.959. Abbreviations: <t>TMB,</t> <t>3,3′,5,5′-tetramethylbenzidine;</t> SWNT, single-walled carbon nanotubes.
    Tmb Substrate Reagent Set, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tmb substrate reagent set/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tmb substrate reagent set - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson ii flow cytometer system
    Assessment of autophagy. ( A ) Histogram of <t>flow</t> cytometric analysis of mutant cybrids (III-8.48) and control cybrids (C59.32) using CYTOID® Autophagy Detection Kit. Three control and three mutant cybrids were incubated with DMEM in the absence and presence of rapamycin (inducers of autophagy) and chloroquine (lysosomal inhibitor) at 37°C for 18 h, added to CYTO-ID ® -Green dye and analyzed using a Novocyte flow <t>cytometer</t> (ACEA Biosciences). ( B ) Relative ratios of Cyto-ID fluorescence intensity from three mutant and three control cell lines. Three independent determinations were done in each cell line. ( C ) Western blot analysis for autophagic response proteins: <t>LC3-I/II</t> and p62. Twenty micrograms of total cellular proteins from various cell lines were electrophoresed, electroblotted and hybridized with LC3, p62 and with β -actin as a loading control. ( D ) Quantification of autophagy markers LC3 I/II and p62 in mutant and control cell lines were determined as described elsewhere ( 70 ). Three independent determinations were done in each cell line. G. Graph details and symbols are explained in the legend to Figure 4 .
    Ii Flow Cytometer System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ii flow cytometer system/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ii flow cytometer system - by Bioz Stars, 2021-03
    86/100 stars
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    86
    Becton Dickinson h2 o2
    Assessment of autophagy. ( A ) Histogram of <t>flow</t> cytometric analysis of mutant cybrids (III-8.48) and control cybrids (C59.32) using CYTOID® Autophagy Detection Kit. Three control and three mutant cybrids were incubated with DMEM in the absence and presence of rapamycin (inducers of autophagy) and chloroquine (lysosomal inhibitor) at 37°C for 18 h, added to CYTO-ID ® -Green dye and analyzed using a Novocyte flow <t>cytometer</t> (ACEA Biosciences). ( B ) Relative ratios of Cyto-ID fluorescence intensity from three mutant and three control cell lines. Three independent determinations were done in each cell line. ( C ) Western blot analysis for autophagic response proteins: <t>LC3-I/II</t> and p62. Twenty micrograms of total cellular proteins from various cell lines were electrophoresed, electroblotted and hybridized with LC3, p62 and with β -actin as a loading control. ( D ) Quantification of autophagy markers LC3 I/II and p62 in mutant and control cell lines were determined as described elsewhere ( 70 ). Three independent determinations were done in each cell line. G. Graph details and symbols are explained in the legend to Figure 4 .
    H2 O2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Binding of the SWNT antibody complex to its specific target. ( A ) Typical saturation curve. ( B ) Lineweaver–Burk plots. The data derived from the saturation curve were fitted to the dashed line (see text). The line represents 1/V max (obtained from ordinate intercept) and 1/K m (obtained from abscissa intercept). 1/ v = 0.007373 (1/[SWNT]) + 0.000812, R 2 = 0.999. V max,SWNT = 1231, K m,SWNT = 9.09 (μg/mL). ( C ) Hill plots. Slope of the line represents n H = 0.959. Abbreviations: TMB, 3,3′,5,5′-tetramethylbenzidine; SWNT, single-walled carbon nanotubes.

    Journal: International Journal of Nanomedicine

    Article Title: Preparation and binding study of a complex made of DNA-treated single-walled carbon nanotubes and antibody for specific delivery of a "molecular heater" platform

    doi: 10.2147/IJN.S34202

    Figure Lengend Snippet: Binding of the SWNT antibody complex to its specific target. ( A ) Typical saturation curve. ( B ) Lineweaver–Burk plots. The data derived from the saturation curve were fitted to the dashed line (see text). The line represents 1/V max (obtained from ordinate intercept) and 1/K m (obtained from abscissa intercept). 1/ v = 0.007373 (1/[SWNT]) + 0.000812, R 2 = 0.999. V max,SWNT = 1231, K m,SWNT = 9.09 (μg/mL). ( C ) Hill plots. Slope of the line represents n H = 0.959. Abbreviations: TMB, 3,3′,5,5′-tetramethylbenzidine; SWNT, single-walled carbon nanotubes.

    Article Snippet: Thereafter, horseradish peroxidase assay was performed using 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate and an excess amount of H2 O2 (TMB substrate reagent set, BD Falcon® ).

    Techniques: Binding Assay, Derivative Assay

    Binding of IgG to its specific target. ( A ) Typical saturation curve. ( B ) Lineweaver–Burk plots. The data derived from the saturation curve were fitted to the dashed line (see text). The line represents 1/V max (obtained from ordinate intercept) and 1/K m (obtained from abscissa intercept). 1/ v = 0.041700 (1/[IgG]) + 0.000719, R 2 = 0.999. V max,IgG = 1391, K m,IgG = 58.0 (ng/mL). ( C ) Hill plots. Slope of the line represents n H = 0.957. Abbreviation: TMB, 3,3′,5,5′-tetramethylbenzidine.

    Journal: International Journal of Nanomedicine

    Article Title: Preparation and binding study of a complex made of DNA-treated single-walled carbon nanotubes and antibody for specific delivery of a "molecular heater" platform

    doi: 10.2147/IJN.S34202

    Figure Lengend Snippet: Binding of IgG to its specific target. ( A ) Typical saturation curve. ( B ) Lineweaver–Burk plots. The data derived from the saturation curve were fitted to the dashed line (see text). The line represents 1/V max (obtained from ordinate intercept) and 1/K m (obtained from abscissa intercept). 1/ v = 0.041700 (1/[IgG]) + 0.000719, R 2 = 0.999. V max,IgG = 1391, K m,IgG = 58.0 (ng/mL). ( C ) Hill plots. Slope of the line represents n H = 0.957. Abbreviation: TMB, 3,3′,5,5′-tetramethylbenzidine.

    Article Snippet: Thereafter, horseradish peroxidase assay was performed using 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate and an excess amount of H2 O2 (TMB substrate reagent set, BD Falcon® ).

    Techniques: Binding Assay, Derivative Assay

    Assessment of autophagy. ( A ) Histogram of flow cytometric analysis of mutant cybrids (III-8.48) and control cybrids (C59.32) using CYTOID® Autophagy Detection Kit. Three control and three mutant cybrids were incubated with DMEM in the absence and presence of rapamycin (inducers of autophagy) and chloroquine (lysosomal inhibitor) at 37°C for 18 h, added to CYTO-ID ® -Green dye and analyzed using a Novocyte flow cytometer (ACEA Biosciences). ( B ) Relative ratios of Cyto-ID fluorescence intensity from three mutant and three control cell lines. Three independent determinations were done in each cell line. ( C ) Western blot analysis for autophagic response proteins: LC3-I/II and p62. Twenty micrograms of total cellular proteins from various cell lines were electrophoresed, electroblotted and hybridized with LC3, p62 and with β -actin as a loading control. ( D ) Quantification of autophagy markers LC3 I/II and p62 in mutant and control cell lines were determined as described elsewhere ( 70 ). Three independent determinations were done in each cell line. G. Graph details and symbols are explained in the legend to Figure 4 .

    Journal: Nucleic Acids Research

    Article Title: A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation

    doi: 10.1093/nar/gkaa1225

    Figure Lengend Snippet: Assessment of autophagy. ( A ) Histogram of flow cytometric analysis of mutant cybrids (III-8.48) and control cybrids (C59.32) using CYTOID® Autophagy Detection Kit. Three control and three mutant cybrids were incubated with DMEM in the absence and presence of rapamycin (inducers of autophagy) and chloroquine (lysosomal inhibitor) at 37°C for 18 h, added to CYTO-ID ® -Green dye and analyzed using a Novocyte flow cytometer (ACEA Biosciences). ( B ) Relative ratios of Cyto-ID fluorescence intensity from three mutant and three control cell lines. Three independent determinations were done in each cell line. ( C ) Western blot analysis for autophagic response proteins: LC3-I/II and p62. Twenty micrograms of total cellular proteins from various cell lines were electrophoresed, electroblotted and hybridized with LC3, p62 and with β -actin as a loading control. ( D ) Quantification of autophagy markers LC3 I/II and p62 in mutant and control cell lines were determined as described elsewhere ( 70 ). Three independent determinations were done in each cell line. G. Graph details and symbols are explained in the legend to Figure 4 .

    Article Snippet: Samples with or without H2 O2 stimulation were analyzed by BD-LSR II flow cytometer system (Beckton Dickson, Inc.), with an excitation at 488 nm and emission at 529 nm.

    Techniques: Mutagenesis, Incubation, Flow Cytometry, Fluorescence, Western Blot