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Agilent technologies h2 o2
H2 O2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2 o2/product/Agilent technologies
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
h2 o2 - by Bioz Stars, 2021-03
86/100 stars

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Related Articles

Incubation:

Article Title: Effects of Extremely Low-Frequency Electromagnetic Fields on Neurogenesis and Cognitive Behavior in an Experimental Model of Hippocampal Injury
Article Snippet: .. Briefly, after paraffin removal by immersion in decreasing grades of ethanol and washing with Tris-buffered saline (TBS; pH = 7.4), endogenous peroxidase was quenched with 0.3% H2 O2 for 30 min. Then, the sections were exposed to autoclave antigen retrieval and incubated in a blocking solution (serum protein-free solution, Dako, Denmark). .. The sections were incubated with mouse monoclonal primary antibody against the neuronal nuclear antigen (NeuN, 1 : 100; Millipore Chemicon International, MAB377) and secondary HRP-conjugated anti-mouse IgG antibody (1 : 200; Abcam, Cambridge, UK).

Article Title: Multivariate analysis of immunohistochemical evaluation of protein expression in pancreatic ductal adenocarcinoma reveals prognostic significance for persistent Smad4 expression only
Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H2 O2 in methanol for 10 min after which sections were pretreated if necessary with ARS pH 9 for 10 min in the autoclave and cooled for 10 min. Before primary antibody application, sections were incubated with Protein Block Serum-Free (Dako Cytomation, Carpenteria, CA, USA). .. Antibody binding was visualized using the PowerVision + Poly-HRP kit (Immunologic, Duiven, The Netherlands) with 3,3-diaminobenzidin (DAB; Sigma-Aldrich, Seelze, Germany) or DAB + (Dako Cytomation) as chromogen.

Article Title: Effect of Quercetin in the 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-Induced Mouse Model of Parkinson's Disease
Article Snippet: Immunohistochemical studies were performed on paraffin-embedded midbrain sections. .. The 30 μ m thick transverse sections were deparaffinized with xylene and refixed with Bouin's solution for 20 min. For inhibition of endogenous peroxidase, the sections were incubated with 0.3% H2 O2 in methanol for 30 min. After rinsing in 10 mM phosphatebuffered saline (PBS), the sections were incubated with normal goat serum (Dako, diluted to 1 : 10) to inhibit nonspecific binding of the antibodies. .. After incubation with the polyclonal anti-4-hydroxy-2-nonenal (HNE) antibody (1 : 400, Alpha Diagnostic International, San Antonio, TX, USA) at 4°C overnight, the sections were treated with biotinylated secondary antibody for 1 h at 37°C, then with streptavidin-peroxidase for 1 h. Subsequently the sections were incubated with 3, 4-diaminobenzidine.

Article Title: STC1 promotes cell apoptosis via NF-κB phospho-P65 Ser536 in cervical cancer cells
Article Snippet: Electrochemiluminescence was performed with a Gel Documentation and Analysis System (image Pro-plus 6.0 Media Cybernetics, Maryland, USA). .. Immunocytochemical staining for NFκB-phospho-P65 Cells were harvested with ice-cold PBS and fixed in ice-cold acetone for 20 min, and then the cells were quenched by 3% H2 O2 in methanol for 15 min. After blocking in a serum-free protein block for 15 min, phospho-P65 antibody was added at a dilution of 1:50 and incubated with the samples for 1 h at 37°C, followed by detection with Dako Envision + HRP Labeled Polymer for 20 min, followed by incubation with chromogen DAB+ for 5 min. .. Statistical analysis All statistical tests were conducted with SPSS 17.0 software (SPSS, Inc., Chicago, IL, USA).

Article Title: KY1022, a small molecule destabilizing Ras via targeting the Wnt/β-catenin pathway, inhibits development of metastatic colorectal cancer
Article Snippet: The fluorescence signal was visualized using confocal microscopy (LSM700, Carl Zeiss) at excitation wave lengths of 488 nm (Alexa Fluor 488), 543 nm (Alexa Fluor 555), or 405 nm (DAPI). .. For peroxidase IHC analysis, sections were blocked by 3.42% H2 O2 and incubated with primary antibody overnight at 4°C, followed by incubation with biotinylated anti-mouse (Dako) or biotinylated anti-rabbit (Dako) secondary antibodies for 1 hour at room temperature. .. The samples were then incubated in ABC kit (Vector Laboratories) for 1 hour, stained with 3, 3′-diaminobenzidine (DAB; Dako) for 3−7 minutes and counterstained with Mayer's hematoxylin (Muto).

Blocking Assay:

Article Title: Effects of Extremely Low-Frequency Electromagnetic Fields on Neurogenesis and Cognitive Behavior in an Experimental Model of Hippocampal Injury
Article Snippet: .. Briefly, after paraffin removal by immersion in decreasing grades of ethanol and washing with Tris-buffered saline (TBS; pH = 7.4), endogenous peroxidase was quenched with 0.3% H2 O2 for 30 min. Then, the sections were exposed to autoclave antigen retrieval and incubated in a blocking solution (serum protein-free solution, Dako, Denmark). .. The sections were incubated with mouse monoclonal primary antibody against the neuronal nuclear antigen (NeuN, 1 : 100; Millipore Chemicon International, MAB377) and secondary HRP-conjugated anti-mouse IgG antibody (1 : 200; Abcam, Cambridge, UK).

Article Title: Multivariate analysis of immunohistochemical evaluation of protein expression in pancreatic ductal adenocarcinoma reveals prognostic significance for persistent Smad4 expression only
Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H2 O2 in methanol for 10 min after which sections were pretreated if necessary with ARS pH 9 for 10 min in the autoclave and cooled for 10 min. Before primary antibody application, sections were incubated with Protein Block Serum-Free (Dako Cytomation, Carpenteria, CA, USA). .. Antibody binding was visualized using the PowerVision + Poly-HRP kit (Immunologic, Duiven, The Netherlands) with 3,3-diaminobenzidin (DAB; Sigma-Aldrich, Seelze, Germany) or DAB + (Dako Cytomation) as chromogen.

Article Title: Human Amniotic Epithelial Cell Transplantation Induces Markers of Alternative Macrophage Activation and Reduces Established Hepatic Fibrosis
Article Snippet: Hepatic macrophages were identified by staining with F4/80 (1∶250; a gift from Dr Richard Kitching, Monash University); T cells with biotinylated CD3 (1∶200; BD Biosciences), CD4 and CD8 (1∶100 and 1∶50, respectively; from Dr R Kitching) and activated HSC or myofibroblasts using α-smooth muscle actin (α-SMA; 1∶5000; Sigma-Aldrich, St Louis, MO). .. Briefly, endogenous peroxidase activity was quenched by adding 0.3–3% H2 O2 (IMM, HLA-G, F4/80, albumin, CD4, CD8, HNF4α) or peroxidase block (Dako; α-SMA, CD3). .. Non-specific binding was minimized with CAS protein blocking solution (Invitrogen, Camarillo, CA).

Article Title: Changes in PD-L1 expression according to tumor infiltrating lymphocytes of acquired EGFR-TKI resistant EGFR-mutant non-small-cell lung cancer
Article Snippet: PD-L1 staining is well validated and in rountine clinical use [ ]. .. The other consecutive sections were stained with CD8 (1:100, clone C8/144B Agilent/Dako, Santa Clara, CA, USA) Briefly, the samples were heated in Tris-EDTA buffer (pH 9.0) at 95°C for 40 min. To block endogenous peroxidase activity, all the sections were treated with 100% methanol containing 0.3% H2 O2 for 15 min. Nonspecific binding of IgG was blocked by using normal rabbit serum (Agilent/Dako). .. The sections were incubated with mouse anti-CD8+ monoclonal abs (Agilent/Dako) overnight at 4°C Then, they were incubated with biotinylated rabbit-anti-mouse secondary Abs (Agilent/DAKO) followed by the incubation with streptavidin-peroxidase complex solution for 30 min.

Article Title: STC1 promotes cell apoptosis via NF-κB phospho-P65 Ser536 in cervical cancer cells
Article Snippet: Electrochemiluminescence was performed with a Gel Documentation and Analysis System (image Pro-plus 6.0 Media Cybernetics, Maryland, USA). .. Immunocytochemical staining for NFκB-phospho-P65 Cells were harvested with ice-cold PBS and fixed in ice-cold acetone for 20 min, and then the cells were quenched by 3% H2 O2 in methanol for 15 min. After blocking in a serum-free protein block for 15 min, phospho-P65 antibody was added at a dilution of 1:50 and incubated with the samples for 1 h at 37°C, followed by detection with Dako Envision + HRP Labeled Polymer for 20 min, followed by incubation with chromogen DAB+ for 5 min. .. Statistical analysis All statistical tests were conducted with SPSS 17.0 software (SPSS, Inc., Chicago, IL, USA).

Activity Assay:

Article Title: Multivariate analysis of immunohistochemical evaluation of protein expression in pancreatic ductal adenocarcinoma reveals prognostic significance for persistent Smad4 expression only
Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H2 O2 in methanol for 10 min after which sections were pretreated if necessary with ARS pH 9 for 10 min in the autoclave and cooled for 10 min. Before primary antibody application, sections were incubated with Protein Block Serum-Free (Dako Cytomation, Carpenteria, CA, USA). .. Antibody binding was visualized using the PowerVision + Poly-HRP kit (Immunologic, Duiven, The Netherlands) with 3,3-diaminobenzidin (DAB; Sigma-Aldrich, Seelze, Germany) or DAB + (Dako Cytomation) as chromogen.

Article Title: Human Amniotic Epithelial Cell Transplantation Induces Markers of Alternative Macrophage Activation and Reduces Established Hepatic Fibrosis
Article Snippet: Hepatic macrophages were identified by staining with F4/80 (1∶250; a gift from Dr Richard Kitching, Monash University); T cells with biotinylated CD3 (1∶200; BD Biosciences), CD4 and CD8 (1∶100 and 1∶50, respectively; from Dr R Kitching) and activated HSC or myofibroblasts using α-smooth muscle actin (α-SMA; 1∶5000; Sigma-Aldrich, St Louis, MO). .. Briefly, endogenous peroxidase activity was quenched by adding 0.3–3% H2 O2 (IMM, HLA-G, F4/80, albumin, CD4, CD8, HNF4α) or peroxidase block (Dako; α-SMA, CD3). .. Non-specific binding was minimized with CAS protein blocking solution (Invitrogen, Camarillo, CA).

Article Title: Changes in PD-L1 expression according to tumor infiltrating lymphocytes of acquired EGFR-TKI resistant EGFR-mutant non-small-cell lung cancer
Article Snippet: PD-L1 staining is well validated and in rountine clinical use [ ]. .. The other consecutive sections were stained with CD8 (1:100, clone C8/144B Agilent/Dako, Santa Clara, CA, USA) Briefly, the samples were heated in Tris-EDTA buffer (pH 9.0) at 95°C for 40 min. To block endogenous peroxidase activity, all the sections were treated with 100% methanol containing 0.3% H2 O2 for 15 min. Nonspecific binding of IgG was blocked by using normal rabbit serum (Agilent/Dako). .. The sections were incubated with mouse anti-CD8+ monoclonal abs (Agilent/Dako) overnight at 4°C Then, they were incubated with biotinylated rabbit-anti-mouse secondary Abs (Agilent/DAKO) followed by the incubation with streptavidin-peroxidase complex solution for 30 min.

Staining:

Article Title: Changes in PD-L1 expression according to tumor infiltrating lymphocytes of acquired EGFR-TKI resistant EGFR-mutant non-small-cell lung cancer
Article Snippet: PD-L1 staining is well validated and in rountine clinical use [ ]. .. The other consecutive sections were stained with CD8 (1:100, clone C8/144B Agilent/Dako, Santa Clara, CA, USA) Briefly, the samples were heated in Tris-EDTA buffer (pH 9.0) at 95°C for 40 min. To block endogenous peroxidase activity, all the sections were treated with 100% methanol containing 0.3% H2 O2 for 15 min. Nonspecific binding of IgG was blocked by using normal rabbit serum (Agilent/Dako). .. The sections were incubated with mouse anti-CD8+ monoclonal abs (Agilent/Dako) overnight at 4°C Then, they were incubated with biotinylated rabbit-anti-mouse secondary Abs (Agilent/DAKO) followed by the incubation with streptavidin-peroxidase complex solution for 30 min.

Article Title: STC1 promotes cell apoptosis via NF-κB phospho-P65 Ser536 in cervical cancer cells
Article Snippet: Electrochemiluminescence was performed with a Gel Documentation and Analysis System (image Pro-plus 6.0 Media Cybernetics, Maryland, USA). .. Immunocytochemical staining for NFκB-phospho-P65 Cells were harvested with ice-cold PBS and fixed in ice-cold acetone for 20 min, and then the cells were quenched by 3% H2 O2 in methanol for 15 min. After blocking in a serum-free protein block for 15 min, phospho-P65 antibody was added at a dilution of 1:50 and incubated with the samples for 1 h at 37°C, followed by detection with Dako Envision + HRP Labeled Polymer for 20 min, followed by incubation with chromogen DAB+ for 5 min. .. Statistical analysis All statistical tests were conducted with SPSS 17.0 software (SPSS, Inc., Chicago, IL, USA).

Binding Assay:

Article Title: Changes in PD-L1 expression according to tumor infiltrating lymphocytes of acquired EGFR-TKI resistant EGFR-mutant non-small-cell lung cancer
Article Snippet: PD-L1 staining is well validated and in rountine clinical use [ ]. .. The other consecutive sections were stained with CD8 (1:100, clone C8/144B Agilent/Dako, Santa Clara, CA, USA) Briefly, the samples were heated in Tris-EDTA buffer (pH 9.0) at 95°C for 40 min. To block endogenous peroxidase activity, all the sections were treated with 100% methanol containing 0.3% H2 O2 for 15 min. Nonspecific binding of IgG was blocked by using normal rabbit serum (Agilent/Dako). .. The sections were incubated with mouse anti-CD8+ monoclonal abs (Agilent/Dako) overnight at 4°C Then, they were incubated with biotinylated rabbit-anti-mouse secondary Abs (Agilent/DAKO) followed by the incubation with streptavidin-peroxidase complex solution for 30 min.

Article Title: Effect of Quercetin in the 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-Induced Mouse Model of Parkinson's Disease
Article Snippet: Immunohistochemical studies were performed on paraffin-embedded midbrain sections. .. The 30 μ m thick transverse sections were deparaffinized with xylene and refixed with Bouin's solution for 20 min. For inhibition of endogenous peroxidase, the sections were incubated with 0.3% H2 O2 in methanol for 30 min. After rinsing in 10 mM phosphatebuffered saline (PBS), the sections were incubated with normal goat serum (Dako, diluted to 1 : 10) to inhibit nonspecific binding of the antibodies. .. After incubation with the polyclonal anti-4-hydroxy-2-nonenal (HNE) antibody (1 : 400, Alpha Diagnostic International, San Antonio, TX, USA) at 4°C overnight, the sections were treated with biotinylated secondary antibody for 1 h at 37°C, then with streptavidin-peroxidase for 1 h. Subsequently the sections were incubated with 3, 4-diaminobenzidine.

Inhibition:

Article Title: Effect of Quercetin in the 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-Induced Mouse Model of Parkinson's Disease
Article Snippet: Immunohistochemical studies were performed on paraffin-embedded midbrain sections. .. The 30 μ m thick transverse sections were deparaffinized with xylene and refixed with Bouin's solution for 20 min. For inhibition of endogenous peroxidase, the sections were incubated with 0.3% H2 O2 in methanol for 30 min. After rinsing in 10 mM phosphatebuffered saline (PBS), the sections were incubated with normal goat serum (Dako, diluted to 1 : 10) to inhibit nonspecific binding of the antibodies. .. After incubation with the polyclonal anti-4-hydroxy-2-nonenal (HNE) antibody (1 : 400, Alpha Diagnostic International, San Antonio, TX, USA) at 4°C overnight, the sections were treated with biotinylated secondary antibody for 1 h at 37°C, then with streptavidin-peroxidase for 1 h. Subsequently the sections were incubated with 3, 4-diaminobenzidine.

Labeling:

Article Title: STC1 promotes cell apoptosis via NF-κB phospho-P65 Ser536 in cervical cancer cells
Article Snippet: Electrochemiluminescence was performed with a Gel Documentation and Analysis System (image Pro-plus 6.0 Media Cybernetics, Maryland, USA). .. Immunocytochemical staining for NFκB-phospho-P65 Cells were harvested with ice-cold PBS and fixed in ice-cold acetone for 20 min, and then the cells were quenched by 3% H2 O2 in methanol for 15 min. After blocking in a serum-free protein block for 15 min, phospho-P65 antibody was added at a dilution of 1:50 and incubated with the samples for 1 h at 37°C, followed by detection with Dako Envision + HRP Labeled Polymer for 20 min, followed by incubation with chromogen DAB+ for 5 min. .. Statistical analysis All statistical tests were conducted with SPSS 17.0 software (SPSS, Inc., Chicago, IL, USA).

Immunohistochemistry:

Article Title: KY1022, a small molecule destabilizing Ras via targeting the Wnt/β-catenin pathway, inhibits development of metastatic colorectal cancer
Article Snippet: The fluorescence signal was visualized using confocal microscopy (LSM700, Carl Zeiss) at excitation wave lengths of 488 nm (Alexa Fluor 488), 543 nm (Alexa Fluor 555), or 405 nm (DAPI). .. For peroxidase IHC analysis, sections were blocked by 3.42% H2 O2 and incubated with primary antibody overnight at 4°C, followed by incubation with biotinylated anti-mouse (Dako) or biotinylated anti-rabbit (Dako) secondary antibodies for 1 hour at room temperature. .. The samples were then incubated in ABC kit (Vector Laboratories) for 1 hour, stained with 3, 3′-diaminobenzidine (DAB; Dako) for 3−7 minutes and counterstained with Mayer's hematoxylin (Muto).

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  • 97
    Agilent technologies 3 3 diaminobenzidine 4hcl
    CD163, CD204 and CD206 immunohistochemical staining of lung tissue samples from a non-smoker, a smoker, a mild chronic obstructive pulmonary disease patient (COPD), and a very severe COPD. (A) Positive reactivity was identified by <t>3–3′-diaminobenzidine-4HCl</t> (DAB). Bar: 50 µm. (B) Double immunohistochemical analysis for CD163/CD204, CD163/CD206 and CD206/CD204. Lung tissues were obtained from a very severe COPD. CD163 (brown)/CD204 (green), CD163 (brown)/CD206 (green) and CD206 (brown)/CD204 (green) were double stained with DAB (brown) and HistoGreen (green), respectively. Bar: 50 µm.
    3 3 Diaminobenzidine 4hcl, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine 4hcl/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine 4hcl - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    97
    Agilent technologies diaminobenzidine h2 o2
    In situ expression of ORM1 in OM and DF. Tissue sections from different samples of OM ( A , B ) and DF ( C , D ) were incubated with a monoclonal antibody against ORM1, then, with a biotinylated antimouse antibody, and finally with the streptavidine/peroxidase complex. The reaction products were visualized by the incubation with <t>3,3´-diaminobenzidine-H</t> 2 O 2 substrate. Finally, samples were analyzed by optic microscopy (20X). Insets show magnifications (40X) of the marked areas. Arrows indicate endothelial cells of blood vessels (B-D). Panel D also showed contaminant epithelial cells positive for ORM1.
    Diaminobenzidine H2 O2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diaminobenzidine h2 o2/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine h2 o2 - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    86
    Agilent technologies monoclonal mouse anti human cd68
    Serial sections of adipose tissue stained for <t>CD68</t> and GLUT4. CD68 + macrophage expression with immunohistochemistry ( a ). CD68 + positive macrophages are brown coloured (DAB) in a and detected using computerized morphometry (red coloured as depicted in panel b , negative cells depicted in blue). Green arrows indicate CD68 + macrophages. An algorithm was used to quantify the amount of infiltrating macrophages. GLUT4 expression is brown coloured on the cell surface of adipocytes ( c ).
    Monoclonal Mouse Anti Human Cd68, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti human cd68/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti human cd68 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Agilent technologies h2 o2
    Serial sections of adipose tissue stained for <t>CD68</t> and GLUT4. CD68 + macrophage expression with immunohistochemistry ( a ). CD68 + positive macrophages are brown coloured (DAB) in a and detected using computerized morphometry (red coloured as depicted in panel b , negative cells depicted in blue). Green arrows indicate CD68 + macrophages. An algorithm was used to quantify the amount of infiltrating macrophages. GLUT4 expression is brown coloured on the cell surface of adipocytes ( c ).
    H2 O2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    CD163, CD204 and CD206 immunohistochemical staining of lung tissue samples from a non-smoker, a smoker, a mild chronic obstructive pulmonary disease patient (COPD), and a very severe COPD. (A) Positive reactivity was identified by 3–3′-diaminobenzidine-4HCl (DAB). Bar: 50 µm. (B) Double immunohistochemical analysis for CD163/CD204, CD163/CD206 and CD206/CD204. Lung tissues were obtained from a very severe COPD. CD163 (brown)/CD204 (green), CD163 (brown)/CD206 (green) and CD206 (brown)/CD204 (green) were double stained with DAB (brown) and HistoGreen (green), respectively. Bar: 50 µm.

    Journal: PLoS ONE

    Article Title: Overexpression of CD163, CD204 and CD206 on Alveolar Macrophages in the Lungs of Patients with Severe Chronic Obstructive Pulmonary Disease

    doi: 10.1371/journal.pone.0087400

    Figure Lengend Snippet: CD163, CD204 and CD206 immunohistochemical staining of lung tissue samples from a non-smoker, a smoker, a mild chronic obstructive pulmonary disease patient (COPD), and a very severe COPD. (A) Positive reactivity was identified by 3–3′-diaminobenzidine-4HCl (DAB). Bar: 50 µm. (B) Double immunohistochemical analysis for CD163/CD204, CD163/CD206 and CD206/CD204. Lung tissues were obtained from a very severe COPD. CD163 (brown)/CD204 (green), CD163 (brown)/CD206 (green) and CD206 (brown)/CD204 (green) were double stained with DAB (brown) and HistoGreen (green), respectively. Bar: 50 µm.

    Article Snippet: After the removal of non-reacted secondary antibodies, the samples were incubated with 3,3′-diaminobenzidine-4HCl (DAB, Dako, Tokyo, Japan)-H2 O2 solution to visualize the immunolabeling.

    Techniques: Immunohistochemistry, Staining

    In situ expression of ORM1 in OM and DF. Tissue sections from different samples of OM ( A , B ) and DF ( C , D ) were incubated with a monoclonal antibody against ORM1, then, with a biotinylated antimouse antibody, and finally with the streptavidine/peroxidase complex. The reaction products were visualized by the incubation with 3,3´-diaminobenzidine-H 2 O 2 substrate. Finally, samples were analyzed by optic microscopy (20X). Insets show magnifications (40X) of the marked areas. Arrows indicate endothelial cells of blood vessels (B-D). Panel D also showed contaminant epithelial cells positive for ORM1.

    Journal: Proteome Science

    Article Title: The orosomucoid 1 protein (?1 acid glycoprotein) is overexpressed in odontogenic myxoma

    doi: 10.1186/1477-5956-10-49

    Figure Lengend Snippet: In situ expression of ORM1 in OM and DF. Tissue sections from different samples of OM ( A , B ) and DF ( C , D ) were incubated with a monoclonal antibody against ORM1, then, with a biotinylated antimouse antibody, and finally with the streptavidine/peroxidase complex. The reaction products were visualized by the incubation with 3,3´-diaminobenzidine-H 2 O 2 substrate. Finally, samples were analyzed by optic microscopy (20X). Insets show magnifications (40X) of the marked areas. Arrows indicate endothelial cells of blood vessels (B-D). Panel D also showed contaminant epithelial cells positive for ORM1.

    Article Snippet: The reaction products were visualized by incubation with 3,3´-diaminobenzidine-H2 O2 as substrate (Dako Corporation, USA).

    Techniques: In Situ, Expressing, Incubation, Microscopy

    Serial sections of adipose tissue stained for CD68 and GLUT4. CD68 + macrophage expression with immunohistochemistry ( a ). CD68 + positive macrophages are brown coloured (DAB) in a and detected using computerized morphometry (red coloured as depicted in panel b , negative cells depicted in blue). Green arrows indicate CD68 + macrophages. An algorithm was used to quantify the amount of infiltrating macrophages. GLUT4 expression is brown coloured on the cell surface of adipocytes ( c ).

    Journal: Molecular Imaging and Biology

    Article Title: [18F]FDG Uptake in Adipose Tissue Is Not Related to Inflammation in Type 2 Diabetes Mellitus

    doi: 10.1007/s11307-020-01538-0

    Figure Lengend Snippet: Serial sections of adipose tissue stained for CD68 and GLUT4. CD68 + macrophage expression with immunohistochemistry ( a ). CD68 + positive macrophages are brown coloured (DAB) in a and detected using computerized morphometry (red coloured as depicted in panel b , negative cells depicted in blue). Green arrows indicate CD68 + macrophages. An algorithm was used to quantify the amount of infiltrating macrophages. GLUT4 expression is brown coloured on the cell surface of adipocytes ( c ).

    Article Snippet: After cooling down, endogenous peroxidase activity was blocked in H2 O2 0.03 % for 30 min and sections were then incubated with Monoclonal Mouse Anti-Human CD68 (PMG1, 1:250 DAKO) for 60 min at room temperature or with anti-GLUT4 antibody (Novus, pAb no. NBP1-49533, 1:100) overnight at 4 °C.

    Techniques: Staining, Expressing, Immunohistochemistry