h2 o2  (Abcam)

 
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  • 99
    Name:
    H2O2 Standard
    Description:

    Catalog Number:
    ab211487
    Price:
    None
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    Structured Review

    Abcam h2 o2

    https://www.bioz.com/result/h2 o2/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Cocaine promotes oxidative stress and microglial-macrophage activation in rat cerebellum
    Article Snippet: CD68 (ED1) Immunohistochemistry Cerebellar CD68 expression was examined by immunohistochemistry. .. Sections were rinsed with 0.01M PBS, pH 7.0 and blocked with 30% H2 O2 for 20 min followed by incubation overnight with a primary rabbit anti-CD68 (ED1) (Abcam, Cambridge, UK; dilution 1:500 in PBS with 0.3% Triton X-100 and 5% normal goat serum). .. Sections were rinsed in PBS and incubated at room temperature shaking for 1 h in 0.4% biotinylated anti-rabbit IgG.

    Article Title: Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke. Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke
    Article Snippet: After deparaffinization and rehydration, the sections were incubated for 15 min at 95°C in 0.01 mol/L citrate buffer (pH 6.0) for antigen retrieval. .. Following incubation in 0.3% H2 O2 followed by 5% bovine serum albumin to avoid nonspecific immunoreactions, the sections were stained overnight at 4°C using anti‐NeuN antibody (ab104224; Abcam). .. Then, the sections were washed with 0.01 mol/L PBS and later incubated using a corresponding secondary antibody (8125; Cell Signaling Technology) for 1 hr at room temperature; the immunoreactivity was visualized by treatment with Dako Envision kit HRP (K4006; DAKO).

    Article Title: The regulation and function of microRNA-377/RASSF8 signaling axis in gastric cancer
    Article Snippet: .. Following blocking of endogenous peroxidase activity with 3% H2 O2 for 15 min at 25°C, the sections were incubated with a primary antibody directed against RASSF8 (dilution, 1:300; cat. no. ab214324; Abcam, Cambridge, MA, USA) overnight at 4°C. .. Following washing with PBS for 3 times (10 min each, 25°C), the specimens were incubated with F(ab')2-goat anti-rabbit immunoglobulin G (IgG) (H+L) secondary antibody (dilution, 1:200; cat. no. Q-11401MP; Thermo Fisher Scientific Inc., Waltham, MA, USA) at 37°C for 1 h and subsequently washed with PBS three times.

    Article Title: LINC01088 inhibits tumorigenesis of ovarian epithelial cells by targeting miR-24-1-5p
    Article Snippet: After DNA sequencing, recombinant pMIR-PAK4 or pMIR-LINC01088 plasmids were co-transfected with pcDNA6.2-GW/EmGFP-miR plasmids containing miR-24-1-5p and PRL-TK plasmids (10:1:0.1) into A2780 cells using Lipofectmine3000 and incubated for 48 h. Then luciferase reporter gene assay was conducted by using the Dual-Luciferase Reporter Assay System (Promega, USA). .. Immunohistochemistry Formalin-fixed, paraffin-embedded tissue sections were deparaffinized, hydrated and soaked in 3% H2 O2 at room temperature for 10 min. Then the slides were incubated with rabbit anti-human PAK4 antibody (10 µg/ml, Abcam, UK) overnight at 4 °C in a humidified chamber. .. The next day, biotin-labeled sheep anti-rabbit IgG antibody was added to sections and incubated at 37 °C for 10 to 30 min. Then, the HRP streptavidin solution was added and the slides were incubated at 37 °C for 10 to 30 min.

    Article Title: Effect of maternal fluoxetine exposure on lung, heart, and kidney development in rat neonates
    Article Snippet: Some tissue sections were incubated with HoxB5 antibody (Bioss Company) at a 1:150 concentration and some tissue sections were incubated with SPC antibody (Bioss Company) at a 1:150 concentration overnight at 4 °C. .. After rinsing the slides by TBS with Triton X-100 and H2 O2 in PBS, the slides were incubated with Goat Anti-Rabbit IgG (Abcam Company) (1:1000 concentration) for 1 hr at room temperature, then they were stained with DAB 3% for 10 min and after rinsing were counterstained with hematoxylin. .. The stained slides were studied under a light microscope (Olympus BX51) equipped with camera (Olympus DP25) and Cell software.

    Article Title: Laminin signals initiate the reciprocal loop that informs breast-specific gene expression and homeostasis by activating NO, p53 and microRNAs
    Article Snippet: Slides were deparaffinized, hydrated, and treated with antigen unmasking solutions (Vector Laboratories, Inc.). .. After being blocked with 0.3% H2 O2 and nonimmune goat serum, sections were incubated at room temperature with an antibody against S-nitrosocysteine (Abcam, Cambridge, MA, USA, clone HY8E12), human LAMA3 (R & D Systems, Minneapolis, MN, USA, , clone 546215) or wild-type human p53 (EMD Millipore, clone pAb1620) and link antibodies, followed by peroxidase-conjugated streptavidin complex and diaminobenzidine tetrahydroxy chloride solution as the peroxidase substrate (Vector Laboratories, Burlingame, CA, USA). .. Photomicrographs were taken with the Zeiss Axioskop Imaging platform and Axion Vision software (Version 4.7).

    Staining:

    Article Title: Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke. Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke
    Article Snippet: After deparaffinization and rehydration, the sections were incubated for 15 min at 95°C in 0.01 mol/L citrate buffer (pH 6.0) for antigen retrieval. .. Following incubation in 0.3% H2 O2 followed by 5% bovine serum albumin to avoid nonspecific immunoreactions, the sections were stained overnight at 4°C using anti‐NeuN antibody (ab104224; Abcam). .. Then, the sections were washed with 0.01 mol/L PBS and later incubated using a corresponding secondary antibody (8125; Cell Signaling Technology) for 1 hr at room temperature; the immunoreactivity was visualized by treatment with Dako Envision kit HRP (K4006; DAKO).

    Article Title: Effect of maternal fluoxetine exposure on lung, heart, and kidney development in rat neonates
    Article Snippet: Some tissue sections were incubated with HoxB5 antibody (Bioss Company) at a 1:150 concentration and some tissue sections were incubated with SPC antibody (Bioss Company) at a 1:150 concentration overnight at 4 °C. .. After rinsing the slides by TBS with Triton X-100 and H2 O2 in PBS, the slides were incubated with Goat Anti-Rabbit IgG (Abcam Company) (1:1000 concentration) for 1 hr at room temperature, then they were stained with DAB 3% for 10 min and after rinsing were counterstained with hematoxylin. .. The stained slides were studied under a light microscope (Olympus BX51) equipped with camera (Olympus DP25) and Cell software.

    Blocking Assay:

    Article Title: The regulation and function of microRNA-377/RASSF8 signaling axis in gastric cancer
    Article Snippet: .. Following blocking of endogenous peroxidase activity with 3% H2 O2 for 15 min at 25°C, the sections were incubated with a primary antibody directed against RASSF8 (dilution, 1:300; cat. no. ab214324; Abcam, Cambridge, MA, USA) overnight at 4°C. .. Following washing with PBS for 3 times (10 min each, 25°C), the specimens were incubated with F(ab')2-goat anti-rabbit immunoglobulin G (IgG) (H+L) secondary antibody (dilution, 1:200; cat. no. Q-11401MP; Thermo Fisher Scientific Inc., Waltham, MA, USA) at 37°C for 1 h and subsequently washed with PBS three times.

    Activity Assay:

    Article Title: The regulation and function of microRNA-377/RASSF8 signaling axis in gastric cancer
    Article Snippet: .. Following blocking of endogenous peroxidase activity with 3% H2 O2 for 15 min at 25°C, the sections were incubated with a primary antibody directed against RASSF8 (dilution, 1:300; cat. no. ab214324; Abcam, Cambridge, MA, USA) overnight at 4°C. .. Following washing with PBS for 3 times (10 min each, 25°C), the specimens were incubated with F(ab')2-goat anti-rabbit immunoglobulin G (IgG) (H+L) secondary antibody (dilution, 1:200; cat. no. Q-11401MP; Thermo Fisher Scientific Inc., Waltham, MA, USA) at 37°C for 1 h and subsequently washed with PBS three times.

    Article Title: Aging Alters Cerebrovascular Endothelial GPCR and K+ Channel Function: Divergent Role of Biological Sex
    Article Snippet: In between individual applications, including the NS309 concentration‐response experiments, the endothelial tube was washed with PSS to baseline conditions. .. Catalase Activity Assays In accord with similar [Ca2+ ]i responses to H2 O2 in young versus old C57BL/6 animals, enzymatic activity decomposing H2 O2 into H2 O was evaluated using the Catalase Activity Assay Kit (Abcam, Cambridge, UK) in accord with manufacturer’s instructions with modification of H2 O2 applied (0.1 mmol/L instead of 1 mmol/L H2 O2 ). .. In brief, cerebral blood vessels were isolated from the brain using an illustrated protocol ( ).

    Modification:

    Article Title: Aging Alters Cerebrovascular Endothelial GPCR and K+ Channel Function: Divergent Role of Biological Sex
    Article Snippet: In between individual applications, including the NS309 concentration‐response experiments, the endothelial tube was washed with PSS to baseline conditions. .. Catalase Activity Assays In accord with similar [Ca2+ ]i responses to H2 O2 in young versus old C57BL/6 animals, enzymatic activity decomposing H2 O2 into H2 O was evaluated using the Catalase Activity Assay Kit (Abcam, Cambridge, UK) in accord with manufacturer’s instructions with modification of H2 O2 applied (0.1 mmol/L instead of 1 mmol/L H2 O2 ). .. In brief, cerebral blood vessels were isolated from the brain using an illustrated protocol ( ).

    other:

    Article Title: HMGB1 enhances smooth muscle cell proliferation and migration in pulmonary artery remodeling
    Article Snippet: Xie J, Zhou X, Hu X, Jiang H. H2O2 evokes injury of cardiomyocytes through upregulating HMGB1.

    Immunohistochemistry:

    Article Title: LINC01088 inhibits tumorigenesis of ovarian epithelial cells by targeting miR-24-1-5p
    Article Snippet: After DNA sequencing, recombinant pMIR-PAK4 or pMIR-LINC01088 plasmids were co-transfected with pcDNA6.2-GW/EmGFP-miR plasmids containing miR-24-1-5p and PRL-TK plasmids (10:1:0.1) into A2780 cells using Lipofectmine3000 and incubated for 48 h. Then luciferase reporter gene assay was conducted by using the Dual-Luciferase Reporter Assay System (Promega, USA). .. Immunohistochemistry Formalin-fixed, paraffin-embedded tissue sections were deparaffinized, hydrated and soaked in 3% H2 O2 at room temperature for 10 min. Then the slides were incubated with rabbit anti-human PAK4 antibody (10 µg/ml, Abcam, UK) overnight at 4 °C in a humidified chamber. .. The next day, biotin-labeled sheep anti-rabbit IgG antibody was added to sections and incubated at 37 °C for 10 to 30 min. Then, the HRP streptavidin solution was added and the slides were incubated at 37 °C for 10 to 30 min.

    Formalin-fixed Paraffin-Embedded:

    Article Title: LINC01088 inhibits tumorigenesis of ovarian epithelial cells by targeting miR-24-1-5p
    Article Snippet: After DNA sequencing, recombinant pMIR-PAK4 or pMIR-LINC01088 plasmids were co-transfected with pcDNA6.2-GW/EmGFP-miR plasmids containing miR-24-1-5p and PRL-TK plasmids (10:1:0.1) into A2780 cells using Lipofectmine3000 and incubated for 48 h. Then luciferase reporter gene assay was conducted by using the Dual-Luciferase Reporter Assay System (Promega, USA). .. Immunohistochemistry Formalin-fixed, paraffin-embedded tissue sections were deparaffinized, hydrated and soaked in 3% H2 O2 at room temperature for 10 min. Then the slides were incubated with rabbit anti-human PAK4 antibody (10 µg/ml, Abcam, UK) overnight at 4 °C in a humidified chamber. .. The next day, biotin-labeled sheep anti-rabbit IgG antibody was added to sections and incubated at 37 °C for 10 to 30 min. Then, the HRP streptavidin solution was added and the slides were incubated at 37 °C for 10 to 30 min.

    Concentration Assay:

    Article Title: Effect of maternal fluoxetine exposure on lung, heart, and kidney development in rat neonates
    Article Snippet: Some tissue sections were incubated with HoxB5 antibody (Bioss Company) at a 1:150 concentration and some tissue sections were incubated with SPC antibody (Bioss Company) at a 1:150 concentration overnight at 4 °C. .. After rinsing the slides by TBS with Triton X-100 and H2 O2 in PBS, the slides were incubated with Goat Anti-Rabbit IgG (Abcam Company) (1:1000 concentration) for 1 hr at room temperature, then they were stained with DAB 3% for 10 min and after rinsing were counterstained with hematoxylin. .. The stained slides were studied under a light microscope (Olympus BX51) equipped with camera (Olympus DP25) and Cell software.

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  • btg2  (Abcam)
    94
    Abcam btg2
    <t>BTG2</t> protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Btg2, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btg2/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    btg2 - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    h2 o2  (Abcam)
    99
    Abcam h2 o2
    <t>BTG2</t> protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    H2 O2, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Article Snippet: Studies utilizing a set of deletion mutants revealed that several antioxidant-related functions of BTG2 (protection against H2 O2 , association with the NFE2L2 protein, stimulation of antioxidant gene expression) were found to be dependent upon the presence of box B, a highly conserved amino acid sequence within the BTG/TOB protein family.

    Techniques: Activity Assay, Transfection, Plasmid Preparation