pbs  (Vector Laboratories)


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  • 99
    Name:
    ImmEdge Hydrophobic Barrier PAP Pen
    Description:
    ImmEdge Hydrophobic Barrier PAP Pen is an improvement over the PAP pen for immunohistochemistry immunocytochemistry immunofluorescence and in situ hybridization It is designed to provide a heat stable water repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide The ImmEdge Pen barrier is stable for use buffers with and without detergent e g Tween 20 or Triton X 100 etc The pale blue barrier is insoluble in alcohol and acetone but is completely removed by all commonly used xylene and xylene substitute clearing agents The ImmEdge Pen is compatible with enzyme or fluorescence based detection systems The ImmEdge Pen is environmentally friendly it does not contain ozone depleting solvents as identified by the Montreal Protocol or the Council of the European Union specifically fluorocarbons and chlorocarbons
    Catalog Number:
    h-4000
    Price:
    None
    Category:
    Histology reagents or solutions or stains
    Size:
    2 Pen Set
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    Structured Review

    Vector Laboratories pbs
    ImmEdge Hydrophobic Barrier PAP Pen
    ImmEdge Hydrophobic Barrier PAP Pen is an improvement over the PAP pen for immunohistochemistry immunocytochemistry immunofluorescence and in situ hybridization It is designed to provide a heat stable water repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide The ImmEdge Pen barrier is stable for use buffers with and without detergent e g Tween 20 or Triton X 100 etc The pale blue barrier is insoluble in alcohol and acetone but is completely removed by all commonly used xylene and xylene substitute clearing agents The ImmEdge Pen is compatible with enzyme or fluorescence based detection systems The ImmEdge Pen is environmentally friendly it does not contain ozone depleting solvents as identified by the Montreal Protocol or the Council of the European Union specifically fluorocarbons and chlorocarbons
    https://www.bioz.com/result/pbs/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-03
    99/100 stars

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    other:

    Article Title: Intracellular DNA replication and differentiation of Trypanosoma cruzi is asynchronous within individual host cells in vivo at all stages of infection
    Article Snippet: Sections were outlined with a hydrophobic pen then permeabilized in 0.1% TritonX-100/PBS for 5 min and washed 3 times with PBS.

    Incubation:

    Article Title: Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species
    Article Snippet: .. Pancreas sections were then circumscribed with an ImmEdge Hydrophobic Barrier PenTM (Vector Laboratories, Burlingame, CA) and incubated for a minimum of 10 min with DAKO® Protein Block, Serum Free (Dako; Burlington, Canada) at room temperature in a humid chamber. ..

    Blocking Assay:

    Article Title: Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species
    Article Snippet: .. Pancreas sections were then circumscribed with an ImmEdge Hydrophobic Barrier PenTM (Vector Laboratories, Burlingame, CA) and incubated for a minimum of 10 min with DAKO® Protein Block, Serum Free (Dako; Burlington, Canada) at room temperature in a humid chamber. ..

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  • 98
    Vector Laboratories rabbit anti klotho
    Transplantation of BMCs from <t>Klotho</t> transgenic mice increases Klotho delivery to mdx muscles and increases satellite cell numbers, muscle fiber size and <t>CD206+</t> M2 macrophages. ( A ) QPCR analysis of Klotho expression in tibialis anterior muscles of mdx mice that received BMT from wild-type mice or from Klotho transgenic mice show BMT transplantation from transgenic mice causes a 32-fold greater level of Klotho expression in the muscle. For all panels in Figure 5, white bars are data from mdx mice receiving wild-type BMT. Black bars are data from mdx mice receiving Klotho transgenic BMT. ( B,C ) BMT of Klotho transgenic BMCs caused significantly higher numbers of satellite cells per muscle fiber (B) and per unit volume of muscle (C), compared with mdx mice receiving wild-type BMT. ( D,E ) BMT of Klotho transgenic BMCs caused significantly larger muscle fiber cross-sectional areas, compared with mdx mice receiving wild-type BMT. Data are shown as frequency distribution of fiber cross-sectional areas for tibialis anterior muscles from mdx mice receiving wild-type BMT (white bars) or Klotho transgenic BMT (black bars) (D) and shown as mean value of cross-sectional areas of the same groups (E). ( F ) Transplantation of Klotho transgenic BMCs caused significantly more CD206+ M2 macrophages to occur in mdx muscles at 6 months BMT, compared with mice receiving wild-type BMT. N = 5 per data set. * indicates significant difference from expression in mdx muscles at P
    Rabbit Anti Klotho, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti klotho/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti klotho - by Bioz Stars, 2021-03
    98/100 stars
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    99
    Vector Laboratories vectastain abc hrp kit peroxidase standard
    Transplantation of BMCs from <t>Klotho</t> transgenic mice increases Klotho delivery to mdx muscles and increases satellite cell numbers, muscle fiber size and <t>CD206+</t> M2 macrophages. ( A ) QPCR analysis of Klotho expression in tibialis anterior muscles of mdx mice that received BMT from wild-type mice or from Klotho transgenic mice show BMT transplantation from transgenic mice causes a 32-fold greater level of Klotho expression in the muscle. For all panels in Figure 5, white bars are data from mdx mice receiving wild-type BMT. Black bars are data from mdx mice receiving Klotho transgenic BMT. ( B,C ) BMT of Klotho transgenic BMCs caused significantly higher numbers of satellite cells per muscle fiber (B) and per unit volume of muscle (C), compared with mdx mice receiving wild-type BMT. ( D,E ) BMT of Klotho transgenic BMCs caused significantly larger muscle fiber cross-sectional areas, compared with mdx mice receiving wild-type BMT. Data are shown as frequency distribution of fiber cross-sectional areas for tibialis anterior muscles from mdx mice receiving wild-type BMT (white bars) or Klotho transgenic BMT (black bars) (D) and shown as mean value of cross-sectional areas of the same groups (E). ( F ) Transplantation of Klotho transgenic BMCs caused significantly more CD206+ M2 macrophages to occur in mdx muscles at 6 months BMT, compared with mice receiving wild-type BMT. N = 5 per data set. * indicates significant difference from expression in mdx muscles at P
    Vectastain Abc Hrp Kit Peroxidase Standard, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain abc hrp kit peroxidase standard/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain abc hrp kit peroxidase standard - by Bioz Stars, 2021-03
    99/100 stars
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    Transplantation of BMCs from Klotho transgenic mice increases Klotho delivery to mdx muscles and increases satellite cell numbers, muscle fiber size and CD206+ M2 macrophages. ( A ) QPCR analysis of Klotho expression in tibialis anterior muscles of mdx mice that received BMT from wild-type mice or from Klotho transgenic mice show BMT transplantation from transgenic mice causes a 32-fold greater level of Klotho expression in the muscle. For all panels in Figure 5, white bars are data from mdx mice receiving wild-type BMT. Black bars are data from mdx mice receiving Klotho transgenic BMT. ( B,C ) BMT of Klotho transgenic BMCs caused significantly higher numbers of satellite cells per muscle fiber (B) and per unit volume of muscle (C), compared with mdx mice receiving wild-type BMT. ( D,E ) BMT of Klotho transgenic BMCs caused significantly larger muscle fiber cross-sectional areas, compared with mdx mice receiving wild-type BMT. Data are shown as frequency distribution of fiber cross-sectional areas for tibialis anterior muscles from mdx mice receiving wild-type BMT (white bars) or Klotho transgenic BMT (black bars) (D) and shown as mean value of cross-sectional areas of the same groups (E). ( F ) Transplantation of Klotho transgenic BMCs caused significantly more CD206+ M2 macrophages to occur in mdx muscles at 6 months BMT, compared with mice receiving wild-type BMT. N = 5 per data set. * indicates significant difference from expression in mdx muscles at P

    Journal: Human Molecular Genetics

    Article Title: Macrophages escape Klotho gene silencing in the mdx mouse model of Duchenne muscular dystrophy and promote muscle growth and increase satellite cell numbers through a Klotho-mediated pathway

    doi: 10.1093/hmg/ddx380

    Figure Lengend Snippet: Transplantation of BMCs from Klotho transgenic mice increases Klotho delivery to mdx muscles and increases satellite cell numbers, muscle fiber size and CD206+ M2 macrophages. ( A ) QPCR analysis of Klotho expression in tibialis anterior muscles of mdx mice that received BMT from wild-type mice or from Klotho transgenic mice show BMT transplantation from transgenic mice causes a 32-fold greater level of Klotho expression in the muscle. For all panels in Figure 5, white bars are data from mdx mice receiving wild-type BMT. Black bars are data from mdx mice receiving Klotho transgenic BMT. ( B,C ) BMT of Klotho transgenic BMCs caused significantly higher numbers of satellite cells per muscle fiber (B) and per unit volume of muscle (C), compared with mdx mice receiving wild-type BMT. ( D,E ) BMT of Klotho transgenic BMCs caused significantly larger muscle fiber cross-sectional areas, compared with mdx mice receiving wild-type BMT. Data are shown as frequency distribution of fiber cross-sectional areas for tibialis anterior muscles from mdx mice receiving wild-type BMT (white bars) or Klotho transgenic BMT (black bars) (D) and shown as mean value of cross-sectional areas of the same groups (E). ( F ) Transplantation of Klotho transgenic BMCs caused significantly more CD206+ M2 macrophages to occur in mdx muscles at 6 months BMT, compared with mice receiving wild-type BMT. N = 5 per data set. * indicates significant difference from expression in mdx muscles at P

    Article Snippet: For identification of Klotho+ or CD206+ cells for bright-field microscopy, acetone-fixed frozen sections of quadriceps were blocked in 3% bovine serum albumin (BSA) and 2% gelatin in 50 mM Tris buffer (pH 7.2) for 1 h and then immunolabeled with rat anti-CD206 (1/50; Serotec) for 3 h at room temperature or with rabbit anti-Klotho (1/50) overnight at 4° C. Sections were washed with PBS and then probed with biotin-conjugated secondary antibodies (1/200; Vector Laboratories) for 30 min.

    Techniques: Transplantation Assay, Transgenic Assay, Mouse Assay, Real-time Polymerase Chain Reaction, Expressing

    Macrophages in dystrophic muscle in vivo and in vitro express Klotho. ( A ) A cross-section of 4-week-old mdx muscle labeled with antibodies to Klotho shows mononucleated cells in an inflammatory lesion express KL (red) although no Klotho is detectible in neighboring, healthy muscle fibers. ( B ) A cross-section of 4-week-old mdx muscle labeled with antibodies to Klotho (red) and CD206 (green). No Klotho is detectible in muscle fibers. Most labeled cells in the inflammatory lesion (between brackets) appear yellow or orange, indicating that they express both Klotho and CD206. CD206+ macrophages (green) that are within the muscle in the perimysium (between arrows), but not within the inflammatory lesion do not express Klotho. Nuclei appear blue (DAPI). Bars = 40 µm. ( C ) Flow cytometry of BMDMs labeled with anti-F4/80 after growth in M-CSF supplemented media show that over 93% of the cells differentiated into mature monocytes/macrophages. ( D ) Western blot of homogenates of BMDMs with anti-Klotho or with anti- Klotho that had been preabsorbed with full length Klotho shows that the 60 kDa protein in macrophages is Klotho. ( E ) Western blot of homogenates of BMDMs that were untreated, or treated with Klotho siRNA or scrambled RNA controls show that the 60 kDa protein in macrophage extracts is Klotho. ( F–H ) BMDMs treated with proinflammatory cytokines TNFα and IFNγ are activated to express elevated levels of TNFα (F), iNOS (G) and a strong trend for increased expression of IFNγ (H). * indicates significant difference from unstimulated control. ( I,J ) BMDMs treated with anti-inflammatory cytokines IL10 and IL4 are activated to express elevated levels of CD206 (I) and CD163 (J). * indicates significant difference from unstimulated control. ( K ) Stimulation of BMDMs with either proinflammatory (Th1; TNFα and IFNγ) or anti-inflammatory (Th2; IL10 and IL4) cytokines increases Klotho expression. * indicates significant difference from unstimulated control at P

    Journal: Human Molecular Genetics

    Article Title: Macrophages escape Klotho gene silencing in the mdx mouse model of Duchenne muscular dystrophy and promote muscle growth and increase satellite cell numbers through a Klotho-mediated pathway

    doi: 10.1093/hmg/ddx380

    Figure Lengend Snippet: Macrophages in dystrophic muscle in vivo and in vitro express Klotho. ( A ) A cross-section of 4-week-old mdx muscle labeled with antibodies to Klotho shows mononucleated cells in an inflammatory lesion express KL (red) although no Klotho is detectible in neighboring, healthy muscle fibers. ( B ) A cross-section of 4-week-old mdx muscle labeled with antibodies to Klotho (red) and CD206 (green). No Klotho is detectible in muscle fibers. Most labeled cells in the inflammatory lesion (between brackets) appear yellow or orange, indicating that they express both Klotho and CD206. CD206+ macrophages (green) that are within the muscle in the perimysium (between arrows), but not within the inflammatory lesion do not express Klotho. Nuclei appear blue (DAPI). Bars = 40 µm. ( C ) Flow cytometry of BMDMs labeled with anti-F4/80 after growth in M-CSF supplemented media show that over 93% of the cells differentiated into mature monocytes/macrophages. ( D ) Western blot of homogenates of BMDMs with anti-Klotho or with anti- Klotho that had been preabsorbed with full length Klotho shows that the 60 kDa protein in macrophages is Klotho. ( E ) Western blot of homogenates of BMDMs that were untreated, or treated with Klotho siRNA or scrambled RNA controls show that the 60 kDa protein in macrophage extracts is Klotho. ( F–H ) BMDMs treated with proinflammatory cytokines TNFα and IFNγ are activated to express elevated levels of TNFα (F), iNOS (G) and a strong trend for increased expression of IFNγ (H). * indicates significant difference from unstimulated control. ( I,J ) BMDMs treated with anti-inflammatory cytokines IL10 and IL4 are activated to express elevated levels of CD206 (I) and CD163 (J). * indicates significant difference from unstimulated control. ( K ) Stimulation of BMDMs with either proinflammatory (Th1; TNFα and IFNγ) or anti-inflammatory (Th2; IL10 and IL4) cytokines increases Klotho expression. * indicates significant difference from unstimulated control at P

    Article Snippet: For identification of Klotho+ or CD206+ cells for bright-field microscopy, acetone-fixed frozen sections of quadriceps were blocked in 3% bovine serum albumin (BSA) and 2% gelatin in 50 mM Tris buffer (pH 7.2) for 1 h and then immunolabeled with rat anti-CD206 (1/50; Serotec) for 3 h at room temperature or with rabbit anti-Klotho (1/50) overnight at 4° C. Sections were washed with PBS and then probed with biotin-conjugated secondary antibodies (1/200; Vector Laboratories) for 30 min.

    Techniques: In Vivo, In Vitro, Labeling, Flow Cytometry, Cytometry, Western Blot, Expressing

    Klotho and CD206 expressing macrophages are co-regulated in vivo . ( A ) QPCR assay of CD206 expression levels in 6-month-old quadriceps muscle from mdx and KL+/ mdx mice. N = 5 per data set. * indicates significant difference from expression in control muscles at P

    Journal: Human Molecular Genetics

    Article Title: Macrophages escape Klotho gene silencing in the mdx mouse model of Duchenne muscular dystrophy and promote muscle growth and increase satellite cell numbers through a Klotho-mediated pathway

    doi: 10.1093/hmg/ddx380

    Figure Lengend Snippet: Klotho and CD206 expressing macrophages are co-regulated in vivo . ( A ) QPCR assay of CD206 expression levels in 6-month-old quadriceps muscle from mdx and KL+/ mdx mice. N = 5 per data set. * indicates significant difference from expression in control muscles at P

    Article Snippet: For identification of Klotho+ or CD206+ cells for bright-field microscopy, acetone-fixed frozen sections of quadriceps were blocked in 3% bovine serum albumin (BSA) and 2% gelatin in 50 mM Tris buffer (pH 7.2) for 1 h and then immunolabeled with rat anti-CD206 (1/50; Serotec) for 3 h at room temperature or with rabbit anti-Klotho (1/50) overnight at 4° C. Sections were washed with PBS and then probed with biotin-conjugated secondary antibodies (1/200; Vector Laboratories) for 30 min.

    Techniques: Expressing, In Vivo, Real-time Polymerase Chain Reaction, Mouse Assay

    Total nucleolin expression in wild-type and cystic fibrosis (CF) mice lungs. Lung sections from ( a , c , e ) wild-type and ( b , d , f ) δF508 C57Bl6 mice were stained with rabbit antinucleolin H-250 antibody, counterstained with hematoxylin, and examined

    Journal: Molecular Therapy

    Article Title: Nucleolin-Mediated Cellular Trafficking of DNA Nanoparticle Is Lipid Raft and Microtubule Dependent and Can Be Modulated by Glucocorticoid

    doi: 10.1038/mt.2010.214

    Figure Lengend Snippet: Total nucleolin expression in wild-type and cystic fibrosis (CF) mice lungs. Lung sections from ( a , c , e ) wild-type and ( b , d , f ) δF508 C57Bl6 mice were stained with rabbit antinucleolin H-250 antibody, counterstained with hematoxylin, and examined

    Article Snippet: Nucleolin was recognized by a rabbit antinucleolin antibody (H-250), and revealed by the NovaRed (Vector Laboratories, Burlingame, CA) substrate for horseradish peroxidase, and nuclei was counterstained blue with hematoxylin.

    Techniques: Expressing, Mouse Assay, Staining