nuclear fast red staining  (Vector Laboratories)


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    Vector Laboratories nuclear fast red staining
    Number of NOS-positive neurons . Examination of nitric oxide synthase- (NOS) positive cells by using NADPH-diaphorase <t>staining</t> compared to all plexus cells counted by <t>nuclear</t> <t>fast</t> <t>red.</t> Similar to the significantly reduced mRNA gene expression the number of NOS-positive neurons showed a significant decline (37.3 ± 9.1 to 20.7 ± 4.2%) in aged donors and verified the alteration of gene expression on protein level. * P ≤ 0.05.
    Nuclear Fast Red Staining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclear fast red staining/product/Vector Laboratories
    Average 97 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    nuclear fast red staining - by Bioz Stars, 2022-09
    97/100 stars

    Images

    1) Product Images from "Age-related gene expression analysis in enteric ganglia of human colon after laser microdissection"

    Article Title: Age-related gene expression analysis in enteric ganglia of human colon after laser microdissection

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2014.00276

    Number of NOS-positive neurons . Examination of nitric oxide synthase- (NOS) positive cells by using NADPH-diaphorase staining compared to all plexus cells counted by nuclear fast red. Similar to the significantly reduced mRNA gene expression the number of NOS-positive neurons showed a significant decline (37.3 ± 9.1 to 20.7 ± 4.2%) in aged donors and verified the alteration of gene expression on protein level. * P ≤ 0.05.
    Figure Legend Snippet: Number of NOS-positive neurons . Examination of nitric oxide synthase- (NOS) positive cells by using NADPH-diaphorase staining compared to all plexus cells counted by nuclear fast red. Similar to the significantly reduced mRNA gene expression the number of NOS-positive neurons showed a significant decline (37.3 ± 9.1 to 20.7 ± 4.2%) in aged donors and verified the alteration of gene expression on protein level. * P ≤ 0.05.

    Techniques Used: Staining, Expressing

    2) Product Images from "Changes in the Intestinal Histomorphometry, the Expression of Intestinal Tight Junction Proteins, and the Bone Structure and Liver of Pre-Laying Hens Following Oral Administration of Fumonisins for 21 Days"

    Article Title: Changes in the Intestinal Histomorphometry, the Expression of Intestinal Tight Junction Proteins, and the Bone Structure and Liver of Pre-Laying Hens Following Oral Administration of Fumonisins for 21 Days

    Journal: Toxins

    doi: 10.3390/toxins13060375

    The effect of fumonisin intoxication on the expression of zonula occludens tight junction protein-1 (ZO-1) and claudin-3 (C-3) proteins in intestine and liver tissue in pre-laying hens. ( A ) Representative pictures of the antibodies’ control for immunohistochemical reactions in the intestine (duodenum) and liver; ( B ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the duodenum; ( C ) the intensity of ZO-1 expression in the duodenum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( D ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the jejunum; ( E ) the intensity of ZO-1 expression in the jejunum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( F ) representative photomicrographs of the immunohistochemical reactions for C-3 in the duodenum; the intensity of C-3 expression in ( G ) the whole duodenum, ( H ) the duodenal crypts, and ( I ) the duodenal villi measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( J ) representative photomicrographs of the immunohistochemical reactions for C-3 in the jejunum; the intensity of C-3 expression in ( K ) the whole jejunum, ( L ) jejunal crypts, and ( M ) jejunal villi, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( N ) representative photomicrographs of the immunohistochemical reactions for C-3 in the liver; ( O ) the intensity of C-3 expression in the liver, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( P ) representative photomicrographs of Goldner’s staining of the liver. Scale bars: A, B, D, F, J, N and P: 200 µm; A, F, J, N: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB). Counterstaining performed with Mayer’s hematoxylin; B, D: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB) with metal enhancer; counterstaining performed with nuclear fast red (NFR). C, E, G, H, I, K, L, M, O: data expressed as mean ± standard error ( n = 8 in each group). Significance was established for fumonisin-intoxicated groups versus the control group (no FB) using a one-way ANOVA followed by a Dunnett’s post-hoc test (normally distributed data) or a Kruskal–Wallis ANOVA with a Dunn’s post-hoc test (for pairwise comparisons with at least one non-normally distributed dataset); * p
    Figure Legend Snippet: The effect of fumonisin intoxication on the expression of zonula occludens tight junction protein-1 (ZO-1) and claudin-3 (C-3) proteins in intestine and liver tissue in pre-laying hens. ( A ) Representative pictures of the antibodies’ control for immunohistochemical reactions in the intestine (duodenum) and liver; ( B ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the duodenum; ( C ) the intensity of ZO-1 expression in the duodenum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( D ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the jejunum; ( E ) the intensity of ZO-1 expression in the jejunum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( F ) representative photomicrographs of the immunohistochemical reactions for C-3 in the duodenum; the intensity of C-3 expression in ( G ) the whole duodenum, ( H ) the duodenal crypts, and ( I ) the duodenal villi measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( J ) representative photomicrographs of the immunohistochemical reactions for C-3 in the jejunum; the intensity of C-3 expression in ( K ) the whole jejunum, ( L ) jejunal crypts, and ( M ) jejunal villi, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( N ) representative photomicrographs of the immunohistochemical reactions for C-3 in the liver; ( O ) the intensity of C-3 expression in the liver, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( P ) representative photomicrographs of Goldner’s staining of the liver. Scale bars: A, B, D, F, J, N and P: 200 µm; A, F, J, N: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB). Counterstaining performed with Mayer’s hematoxylin; B, D: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB) with metal enhancer; counterstaining performed with nuclear fast red (NFR). C, E, G, H, I, K, L, M, O: data expressed as mean ± standard error ( n = 8 in each group). Significance was established for fumonisin-intoxicated groups versus the control group (no FB) using a one-way ANOVA followed by a Dunnett’s post-hoc test (normally distributed data) or a Kruskal–Wallis ANOVA with a Dunn’s post-hoc test (for pairwise comparisons with at least one non-normally distributed dataset); * p

    Techniques Used: Expressing, Immunohistochemistry, Staining

    3) Product Images from "In Vivo Transplantation of Neurosphere-Like Bodies Derived from the Human Postnatal and Adult Enteric Nervous System: A Pilot Study"

    Article Title: In Vivo Transplantation of Neurosphere-Like Bodies Derived from the Human Postnatal and Adult Enteric Nervous System: A Pilot Study

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0093605

    Alterations of the ENS four weeks post-operation. (A, B) Low-magnification bright-field view of NADPH-diaphorase staining demonstrates a visible and relative reduction (i.e. hypoganglionosis) of neuronal cells (shown as %NOS+ of Fast Red+ myenteric plexus cells, dotted lines) as well as neuronal fibers compared to untreated gut control tissues. Note that the musculature is thickened due to hypertrophic effects of BAC as indicated by the white bar. Nuclei are stained red with Nuclear Fast Red dye. (C, D) Compared to the control, hypertrophic effects are also detectable in the ENS as shown by immunohistochemistry for PGP9.5 (arrowheads). Nuclei were stained with DAPI (blue). (E) Quantification of NOS-positive cells in the myenteric plexus, (F) mean ganglia size indicated by PGP9.5 cytoplasmatic immunostaining, and (G) muscle thickness. As seen in box-whisker plots, damage of the ENS and muscle was still quantifiable four weeks after BAC-treatment, however in this analysis, no statistical significant effects were seen by additional cell transplantation compared to the BAC-only treated group. (A–D) Scale bars represent 100 μm. M = mucosa, CM = circular muscle, LM = longitudinal muscle. (E–G) *P
    Figure Legend Snippet: Alterations of the ENS four weeks post-operation. (A, B) Low-magnification bright-field view of NADPH-diaphorase staining demonstrates a visible and relative reduction (i.e. hypoganglionosis) of neuronal cells (shown as %NOS+ of Fast Red+ myenteric plexus cells, dotted lines) as well as neuronal fibers compared to untreated gut control tissues. Note that the musculature is thickened due to hypertrophic effects of BAC as indicated by the white bar. Nuclei are stained red with Nuclear Fast Red dye. (C, D) Compared to the control, hypertrophic effects are also detectable in the ENS as shown by immunohistochemistry for PGP9.5 (arrowheads). Nuclei were stained with DAPI (blue). (E) Quantification of NOS-positive cells in the myenteric plexus, (F) mean ganglia size indicated by PGP9.5 cytoplasmatic immunostaining, and (G) muscle thickness. As seen in box-whisker plots, damage of the ENS and muscle was still quantifiable four weeks after BAC-treatment, however in this analysis, no statistical significant effects were seen by additional cell transplantation compared to the BAC-only treated group. (A–D) Scale bars represent 100 μm. M = mucosa, CM = circular muscle, LM = longitudinal muscle. (E–G) *P

    Techniques Used: Staining, BAC Assay, Immunohistochemistry, Immunostaining, Whisker Assay, Transplantation Assay

    4) Product Images from "Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model"

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    Journal: Journal of Clinical Medicine

    doi: 10.3390/jcm10020272

    ( A ) Representative photomicrographs of the immunohistochemical reactions for leptin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for leptin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for ghrelin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for ghrelin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for marvelD3. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of marvelD3, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); * p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for marvelD3. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of marvelD3, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); * p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for occludin. Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of occludin measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for occludin. Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of occludin measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for vasoactive intestinal peptide (VIP) in Auerbach plexuses (red arrow) of the duodenum and the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of VIP, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for vasoactive intestinal peptide (VIP) in Auerbach plexuses (red arrow) of the duodenum and the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of VIP, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for zonula occludens 1 (ZO-1). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ZO-1, measured by comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for zonula occludens 1 (ZO-1). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ZO-1, measured by comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    5) Product Images from "Identification of alternative protein targets of glutamate-ureido-lysine associated with PSMA tracer uptake in prostate cancer cells"

    Article Title: Identification of alternative protein targets of glutamate-ureido-lysine associated with PSMA tracer uptake in prostate cancer cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2025710119

    Differential expression of NAALADL1 in NEPC and NE-like PC as an alternative target for GUL ligands. ( A ) Schematic of our established PDX mice models of adenocarcinoma (AdPC) and NEPC and alteration of FOLH1 and NAALADL1 gene expression during the transition from AdPC to NEPC. ( B ) The expression of FOLH1 and NAALADL1 in well-established cells lines ( n = 1,377) of DepMap 21Q3 dataset ( 39 ). ( C ) PSMA and NAALADaseL levels in LTL331 and LTL331R models were detected by immunoblotting. TT and 22Rv1 cell lines were used as NAALADaseL- and PSMA-positive control samples, respectively. ( D ) Tissue sections of LTL331 models stained with nuclear fast red to visualize cell nuclei and blue chromogenic substrate used for detection of PSMA and NAALADaseL. ( E ) NAALADL1 gene expression level in different cohorts of metastatic prostate cancer samples. The expression levels are normalized to the mean of entire samples in the cohort ( n = 155). ( F ) Evaluation of the expression of FOLH1 and NAALADL1 genes and their association with AR and NEPC scores in the SU2C/PCF Dream Team Dataset 2019 ( 40 ). The high levels of NAALADL1 gene expression in AdPC are associated with both lower levels of FOLH1 gene expression and higher levels of ENO2 gene expression, the archetypal NE marker. ( G ) Pearson’s correlation between FOLH1 (blue) and NAALADL1 (red) expression levels. ( H ) Pearson’s correlation between ENO2 (blue) and NAALADL1 (red) expression levels among AdPC samples ( n = 199) generated by R2: Genomics Analysis and Visualization Platform ( http://r2.amc.nl ).
    Figure Legend Snippet: Differential expression of NAALADL1 in NEPC and NE-like PC as an alternative target for GUL ligands. ( A ) Schematic of our established PDX mice models of adenocarcinoma (AdPC) and NEPC and alteration of FOLH1 and NAALADL1 gene expression during the transition from AdPC to NEPC. ( B ) The expression of FOLH1 and NAALADL1 in well-established cells lines ( n = 1,377) of DepMap 21Q3 dataset ( 39 ). ( C ) PSMA and NAALADaseL levels in LTL331 and LTL331R models were detected by immunoblotting. TT and 22Rv1 cell lines were used as NAALADaseL- and PSMA-positive control samples, respectively. ( D ) Tissue sections of LTL331 models stained with nuclear fast red to visualize cell nuclei and blue chromogenic substrate used for detection of PSMA and NAALADaseL. ( E ) NAALADL1 gene expression level in different cohorts of metastatic prostate cancer samples. The expression levels are normalized to the mean of entire samples in the cohort ( n = 155). ( F ) Evaluation of the expression of FOLH1 and NAALADL1 genes and their association with AR and NEPC scores in the SU2C/PCF Dream Team Dataset 2019 ( 40 ). The high levels of NAALADL1 gene expression in AdPC are associated with both lower levels of FOLH1 gene expression and higher levels of ENO2 gene expression, the archetypal NE marker. ( G ) Pearson’s correlation between FOLH1 (blue) and NAALADL1 (red) expression levels. ( H ) Pearson’s correlation between ENO2 (blue) and NAALADL1 (red) expression levels among AdPC samples ( n = 199) generated by R2: Genomics Analysis and Visualization Platform ( http://r2.amc.nl ).

    Techniques Used: Expressing, Mouse Assay, Positive Control, Staining, Marker, Generated, Genomic analysis

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    Vector Laboratories nuclear fast red staining
    Number of NOS-positive neurons . Examination of nitric oxide synthase- (NOS) positive cells by using NADPH-diaphorase <t>staining</t> compared to all plexus cells counted by <t>nuclear</t> <t>fast</t> <t>red.</t> Similar to the significantly reduced mRNA gene expression the number of NOS-positive neurons showed a significant decline (37.3 ± 9.1 to 20.7 ± 4.2%) in aged donors and verified the alteration of gene expression on protein level. * P ≤ 0.05.
    Nuclear Fast Red Staining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclear fast red staining/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nuclear fast red staining - by Bioz Stars, 2022-09
    97/100 stars
      Buy from Supplier

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    Number of NOS-positive neurons . Examination of nitric oxide synthase- (NOS) positive cells by using NADPH-diaphorase staining compared to all plexus cells counted by nuclear fast red. Similar to the significantly reduced mRNA gene expression the number of NOS-positive neurons showed a significant decline (37.3 ± 9.1 to 20.7 ± 4.2%) in aged donors and verified the alteration of gene expression on protein level. * P ≤ 0.05.

    Journal: Frontiers in Aging Neuroscience

    Article Title: Age-related gene expression analysis in enteric ganglia of human colon after laser microdissection

    doi: 10.3389/fnagi.2014.00276

    Figure Lengend Snippet: Number of NOS-positive neurons . Examination of nitric oxide synthase- (NOS) positive cells by using NADPH-diaphorase staining compared to all plexus cells counted by nuclear fast red. Similar to the significantly reduced mRNA gene expression the number of NOS-positive neurons showed a significant decline (37.3 ± 9.1 to 20.7 ± 4.2%) in aged donors and verified the alteration of gene expression on protein level. * P ≤ 0.05.

    Article Snippet: Sections were then washed in PBS and nuclear fast red staining (Vector Laboratories Inc., Burlingame, USA) was performed by incubation with the coloring agent for 20 min at room temperature.

    Techniques: Staining, Expressing

    The effect of fumonisin intoxication on the expression of zonula occludens tight junction protein-1 (ZO-1) and claudin-3 (C-3) proteins in intestine and liver tissue in pre-laying hens. ( A ) Representative pictures of the antibodies’ control for immunohistochemical reactions in the intestine (duodenum) and liver; ( B ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the duodenum; ( C ) the intensity of ZO-1 expression in the duodenum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( D ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the jejunum; ( E ) the intensity of ZO-1 expression in the jejunum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( F ) representative photomicrographs of the immunohistochemical reactions for C-3 in the duodenum; the intensity of C-3 expression in ( G ) the whole duodenum, ( H ) the duodenal crypts, and ( I ) the duodenal villi measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( J ) representative photomicrographs of the immunohistochemical reactions for C-3 in the jejunum; the intensity of C-3 expression in ( K ) the whole jejunum, ( L ) jejunal crypts, and ( M ) jejunal villi, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( N ) representative photomicrographs of the immunohistochemical reactions for C-3 in the liver; ( O ) the intensity of C-3 expression in the liver, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( P ) representative photomicrographs of Goldner’s staining of the liver. Scale bars: A, B, D, F, J, N and P: 200 µm; A, F, J, N: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB). Counterstaining performed with Mayer’s hematoxylin; B, D: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB) with metal enhancer; counterstaining performed with nuclear fast red (NFR). C, E, G, H, I, K, L, M, O: data expressed as mean ± standard error ( n = 8 in each group). Significance was established for fumonisin-intoxicated groups versus the control group (no FB) using a one-way ANOVA followed by a Dunnett’s post-hoc test (normally distributed data) or a Kruskal–Wallis ANOVA with a Dunn’s post-hoc test (for pairwise comparisons with at least one non-normally distributed dataset); * p

    Journal: Toxins

    Article Title: Changes in the Intestinal Histomorphometry, the Expression of Intestinal Tight Junction Proteins, and the Bone Structure and Liver of Pre-Laying Hens Following Oral Administration of Fumonisins for 21 Days

    doi: 10.3390/toxins13060375

    Figure Lengend Snippet: The effect of fumonisin intoxication on the expression of zonula occludens tight junction protein-1 (ZO-1) and claudin-3 (C-3) proteins in intestine and liver tissue in pre-laying hens. ( A ) Representative pictures of the antibodies’ control for immunohistochemical reactions in the intestine (duodenum) and liver; ( B ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the duodenum; ( C ) the intensity of ZO-1 expression in the duodenum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( D ) representative photomicrographs of the immunohistochemical reactions for ZO-1 in the jejunum; ( E ) the intensity of ZO-1 expression in the jejunum, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( F ) representative photomicrographs of the immunohistochemical reactions for C-3 in the duodenum; the intensity of C-3 expression in ( G ) the whole duodenum, ( H ) the duodenal crypts, and ( I ) the duodenal villi measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( J ) representative photomicrographs of the immunohistochemical reactions for C-3 in the jejunum; the intensity of C-3 expression in ( K ) the whole jejunum, ( L ) jejunal crypts, and ( M ) jejunal villi, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( N ) representative photomicrographs of the immunohistochemical reactions for C-3 in the liver; ( O ) the intensity of C-3 expression in the liver, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the lower the intensity of the immunoreaction); ( P ) representative photomicrographs of Goldner’s staining of the liver. Scale bars: A, B, D, F, J, N and P: 200 µm; A, F, J, N: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB). Counterstaining performed with Mayer’s hematoxylin; B, D: sections developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB) with metal enhancer; counterstaining performed with nuclear fast red (NFR). C, E, G, H, I, K, L, M, O: data expressed as mean ± standard error ( n = 8 in each group). Significance was established for fumonisin-intoxicated groups versus the control group (no FB) using a one-way ANOVA followed by a Dunnett’s post-hoc test (normally distributed data) or a Kruskal–Wallis ANOVA with a Dunn’s post-hoc test (for pairwise comparisons with at least one non-normally distributed dataset); * p

    Article Snippet: Counterstaining was performed with Mayer’s hematoxylin (MHS32-1L; Sigma-Aldrich, St. Louis, MO, USA) or Nuclear Fast Red counterstain (H-24-2; Vector Laboratories Inc., Burlingame, CA, USA) [ , ].

    Techniques: Expressing, Immunohistochemistry, Staining

    Alterations of the ENS four weeks post-operation. (A, B) Low-magnification bright-field view of NADPH-diaphorase staining demonstrates a visible and relative reduction (i.e. hypoganglionosis) of neuronal cells (shown as %NOS+ of Fast Red+ myenteric plexus cells, dotted lines) as well as neuronal fibers compared to untreated gut control tissues. Note that the musculature is thickened due to hypertrophic effects of BAC as indicated by the white bar. Nuclei are stained red with Nuclear Fast Red dye. (C, D) Compared to the control, hypertrophic effects are also detectable in the ENS as shown by immunohistochemistry for PGP9.5 (arrowheads). Nuclei were stained with DAPI (blue). (E) Quantification of NOS-positive cells in the myenteric plexus, (F) mean ganglia size indicated by PGP9.5 cytoplasmatic immunostaining, and (G) muscle thickness. As seen in box-whisker plots, damage of the ENS and muscle was still quantifiable four weeks after BAC-treatment, however in this analysis, no statistical significant effects were seen by additional cell transplantation compared to the BAC-only treated group. (A–D) Scale bars represent 100 μm. M = mucosa, CM = circular muscle, LM = longitudinal muscle. (E–G) *P

    Journal: PLoS ONE

    Article Title: In Vivo Transplantation of Neurosphere-Like Bodies Derived from the Human Postnatal and Adult Enteric Nervous System: A Pilot Study

    doi: 10.1371/journal.pone.0093605

    Figure Lengend Snippet: Alterations of the ENS four weeks post-operation. (A, B) Low-magnification bright-field view of NADPH-diaphorase staining demonstrates a visible and relative reduction (i.e. hypoganglionosis) of neuronal cells (shown as %NOS+ of Fast Red+ myenteric plexus cells, dotted lines) as well as neuronal fibers compared to untreated gut control tissues. Note that the musculature is thickened due to hypertrophic effects of BAC as indicated by the white bar. Nuclei are stained red with Nuclear Fast Red dye. (C, D) Compared to the control, hypertrophic effects are also detectable in the ENS as shown by immunohistochemistry for PGP9.5 (arrowheads). Nuclei were stained with DAPI (blue). (E) Quantification of NOS-positive cells in the myenteric plexus, (F) mean ganglia size indicated by PGP9.5 cytoplasmatic immunostaining, and (G) muscle thickness. As seen in box-whisker plots, damage of the ENS and muscle was still quantifiable four weeks after BAC-treatment, however in this analysis, no statistical significant effects were seen by additional cell transplantation compared to the BAC-only treated group. (A–D) Scale bars represent 100 μm. M = mucosa, CM = circular muscle, LM = longitudinal muscle. (E–G) *P

    Article Snippet: Samples were counterstained with Nuclear Fast Red solution (Vector Laboratories, Burlingame, USA) for 20 min at room temperature and cover-slipped using Kaiser’s gelatin.

    Techniques: Staining, BAC Assay, Immunohistochemistry, Immunostaining, Whisker Assay, Transplantation Assay

    ( A ) Representative photomicrographs of the immunohistochemical reactions for leptin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Journal: Journal of Clinical Medicine

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    doi: 10.3390/jcm10020272

    Figure Lengend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for leptin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Article Snippet: Counterstaining was performed with Mayer’s hematoxylin (MHS32-1L; Sigma-Aldrich, St. Louis, MO, USA) or Nuclear Fast Red counterstain (H-24-2; Vector Laboratories Inc., Burlingame, CA, USA), respectively.

    Techniques: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for ghrelin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Journal: Journal of Clinical Medicine

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    doi: 10.3390/jcm10020272

    Figure Lengend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for ghrelin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Article Snippet: Counterstaining was performed with Mayer’s hematoxylin (MHS32-1L; Sigma-Aldrich, St. Louis, MO, USA) or Nuclear Fast Red counterstain (H-24-2; Vector Laboratories Inc., Burlingame, CA, USA), respectively.

    Techniques: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for marvelD3. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of marvelD3, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); * p

    Journal: Journal of Clinical Medicine

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    doi: 10.3390/jcm10020272

    Figure Lengend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for marvelD3. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of marvelD3, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); * p

    Article Snippet: Counterstaining was performed with Mayer’s hematoxylin (MHS32-1L; Sigma-Aldrich, St. Louis, MO, USA) or Nuclear Fast Red counterstain (H-24-2; Vector Laboratories Inc., Burlingame, CA, USA), respectively.

    Techniques: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for occludin. Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of occludin measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Journal: Journal of Clinical Medicine

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    doi: 10.3390/jcm10020272

    Figure Lengend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for occludin. Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of occludin measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Article Snippet: Counterstaining was performed with Mayer’s hematoxylin (MHS32-1L; Sigma-Aldrich, St. Louis, MO, USA) or Nuclear Fast Red counterstain (H-24-2; Vector Laboratories Inc., Burlingame, CA, USA), respectively.

    Techniques: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for vasoactive intestinal peptide (VIP) in Auerbach plexuses (red arrow) of the duodenum and the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of VIP, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Journal: Journal of Clinical Medicine

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    doi: 10.3390/jcm10020272

    Figure Lengend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for vasoactive intestinal peptide (VIP) in Auerbach plexuses (red arrow) of the duodenum and the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of VIP, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Article Snippet: Counterstaining was performed with Mayer’s hematoxylin (MHS32-1L; Sigma-Aldrich, St. Louis, MO, USA) or Nuclear Fast Red counterstain (H-24-2; Vector Laboratories Inc., Burlingame, CA, USA), respectively.

    Techniques: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for zonula occludens 1 (ZO-1). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ZO-1, measured by comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Journal: Journal of Clinical Medicine

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    doi: 10.3390/jcm10020272

    Figure Lengend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for zonula occludens 1 (ZO-1). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ZO-1, measured by comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Article Snippet: Counterstaining was performed with Mayer’s hematoxylin (MHS32-1L; Sigma-Aldrich, St. Louis, MO, USA) or Nuclear Fast Red counterstain (H-24-2; Vector Laboratories Inc., Burlingame, CA, USA), respectively.

    Techniques: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY