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Hitachi Ltd h 8100 transmission electron microscope
Characterization of oligomeric Aβ42 preparation. (A) Transmission electron micrograph (magnified×32,000) of 100 µM oAβ42. 10 µL of samples were spotted on a glow-discharged, carbon-coated Formavar grid and incubated for 2 min, washed with distilled water, stained with a 1% (w/v) aqueous uranyl acetate solution, and examined using an <t>H-8100</t> TEM. Scale bar is 100 nm. (B) ThT fluorescence assay of aggregates of Aβ42. a.u., arbitrary unit.
H 8100 Transmission Electron Microscope, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h 8100 transmission electron microscope/product/Hitachi Ltd
Average 89 stars, based on 30 article reviews
Price from $9.99 to $1999.99
h 8100 transmission electron microscope - by Bioz Stars, 2020-08
89/100 stars

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1) Product Images from "Mechanisms of U87 Astrocytoma Cell Uptake and Trafficking of Monomeric versus Protofibril Alzheimer's Disease Amyloid-? Proteins"

Article Title: Mechanisms of U87 Astrocytoma Cell Uptake and Trafficking of Monomeric versus Protofibril Alzheimer's Disease Amyloid-? Proteins

Journal: PLoS ONE

doi: 10.1371/journal.pone.0099939

Characterization of oligomeric Aβ42 preparation. (A) Transmission electron micrograph (magnified×32,000) of 100 µM oAβ42. 10 µL of samples were spotted on a glow-discharged, carbon-coated Formavar grid and incubated for 2 min, washed with distilled water, stained with a 1% (w/v) aqueous uranyl acetate solution, and examined using an H-8100 TEM. Scale bar is 100 nm. (B) ThT fluorescence assay of aggregates of Aβ42. a.u., arbitrary unit.
Figure Legend Snippet: Characterization of oligomeric Aβ42 preparation. (A) Transmission electron micrograph (magnified×32,000) of 100 µM oAβ42. 10 µL of samples were spotted on a glow-discharged, carbon-coated Formavar grid and incubated for 2 min, washed with distilled water, stained with a 1% (w/v) aqueous uranyl acetate solution, and examined using an H-8100 TEM. Scale bar is 100 nm. (B) ThT fluorescence assay of aggregates of Aβ42. a.u., arbitrary unit.

Techniques Used: Transmission Assay, Incubation, Staining, Transmission Electron Microscopy, Fluorescence

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Microscopy:

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Spectrophotometry:

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Transmission Assay:

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Staining:

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    Hitachi Ltd h 8100 transmission electron microscope
    Characterization of oligomeric Aβ42 preparation. (A) Transmission electron micrograph (magnified×32,000) of 100 µM oAβ42. 10 µL of samples were spotted on a glow-discharged, carbon-coated Formavar grid and incubated for 2 min, washed with distilled water, stained with a 1% (w/v) aqueous uranyl acetate solution, and examined using an <t>H-8100</t> TEM. Scale bar is 100 nm. (B) ThT fluorescence assay of aggregates of Aβ42. a.u., arbitrary unit.
    H 8100 Transmission Electron Microscope, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h 8100 transmission electron microscope/product/Hitachi Ltd
    Average 89 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    h 8100 transmission electron microscope - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

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    Characterization of oligomeric Aβ42 preparation. (A) Transmission electron micrograph (magnified×32,000) of 100 µM oAβ42. 10 µL of samples were spotted on a glow-discharged, carbon-coated Formavar grid and incubated for 2 min, washed with distilled water, stained with a 1% (w/v) aqueous uranyl acetate solution, and examined using an H-8100 TEM. Scale bar is 100 nm. (B) ThT fluorescence assay of aggregates of Aβ42. a.u., arbitrary unit.

    Journal: PLoS ONE

    Article Title: Mechanisms of U87 Astrocytoma Cell Uptake and Trafficking of Monomeric versus Protofibril Alzheimer's Disease Amyloid-? Proteins

    doi: 10.1371/journal.pone.0099939

    Figure Lengend Snippet: Characterization of oligomeric Aβ42 preparation. (A) Transmission electron micrograph (magnified×32,000) of 100 µM oAβ42. 10 µL of samples were spotted on a glow-discharged, carbon-coated Formavar grid and incubated for 2 min, washed with distilled water, stained with a 1% (w/v) aqueous uranyl acetate solution, and examined using an H-8100 TEM. Scale bar is 100 nm. (B) ThT fluorescence assay of aggregates of Aβ42. a.u., arbitrary unit.

    Article Snippet: The samples were then stained with 1% uranyl acetate for 1 min, excess fluid was filtered off and the grids were examined with H-8100 transmission electron microscope (Hitachi, Tokyo, Japan) operated at 150 KV and 32,000× magnification.

    Techniques: Transmission Assay, Incubation, Staining, Transmission Electron Microscopy, Fluorescence