Journal: PLoS ONE

Article Title: Inhibition of TMEM16A Expression Suppresses Growth and Invasion in Human Colorectal Cancer Cells

doi: 10.1371/journal.pone.0115443

Figure Lengend Snippet: A, RT-PCR reveals TMEM16A mRNA expression in SW620, HCT116 and LS174T cells, but not in HCT8 and SW480 cells. B, TMEM16A protein expression in HCT8 and SW480, SW620, HCT116 and LS174T cells was detected by Western blotting. Null FRT cells acts as negative control. FRT cells transfected with human TMEM16A serve as positive control.

Article Snippet: The human colorectal carcinoma cell lines HCT8, SW480, SW620, HCT116 and LS174T cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Negative Control, Transfection, Positive Control