guinea pig anti vgat  (Synaptic Systems)


Bioz Manufacturer Symbol Synaptic Systems manufactures this product  
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    Synaptic Systems guinea pig anti vgat
    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. <t>a-f</t> <t>CB1/VGLUT1/DAPI</t> immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l <t>CB1/VGAT/DAPI</t> immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Guinea Pig Anti Vgat, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti vgat/product/Synaptic Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti vgat - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice"

    Article Title: Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05029-7

    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Figure Legend Snippet: Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Techniques Used: Immunolabeling, Comparison

    guinea pig anti vgat  (Synaptic Systems)


    Bioz Manufacturer Symbol Synaptic Systems manufactures this product  
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    Structured Review

    Synaptic Systems guinea pig anti vgat
    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. <t>a-f</t> <t>CB1/VGLUT1/DAPI</t> immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l <t>CB1/VGAT/DAPI</t> immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Guinea Pig Anti Vgat, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti vgat/product/Synaptic Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti vgat - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice"

    Article Title: Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05029-7

    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Figure Legend Snippet: Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Techniques Used: Immunolabeling, Comparison

    guinea pig anti vglut1  (Synaptic Systems)


    Bioz Manufacturer Symbol Synaptic Systems manufactures this product  
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    Structured Review

    Synaptic Systems guinea pig anti vglut1
    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f <t>CB1/VGLUT1/DAPI</t> immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Guinea Pig Anti Vglut1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti vglut1/product/Synaptic Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti vglut1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice"

    Article Title: Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05029-7

    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Figure Legend Snippet: Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Techniques Used: Immunolabeling, Comparison

    guinea pig anti vglut1  (Synaptic Systems)


    Bioz Manufacturer Symbol Synaptic Systems manufactures this product  
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    Structured Review

    Synaptic Systems guinea pig anti vglut1
    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f <t>CB1/VGLUT1/DAPI</t> immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Guinea Pig Anti Vglut1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti vglut1/product/Synaptic Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti vglut1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice"

    Article Title: Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05029-7

    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
    Figure Legend Snippet: Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Techniques Used: Immunolabeling, Comparison

    alexa fluor 488 goat anti guinea pig igg fluorescein isothiocyanate isomer i fitc  (Santa Cruz Biotechnology)

     
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    Structured Review

    Santa Cruz Biotechnology alexa fluor 488 goat anti guinea pig igg fluorescein isothiocyanate isomer i fitc
    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.
    Alexa Fluor 488 Goat Anti Guinea Pig Igg Fluorescein Isothiocyanate Isomer I Fitc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 goat anti guinea pig igg fluorescein isothiocyanate isomer i fitc/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 goat anti guinea pig igg fluorescein isothiocyanate isomer i fitc - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke"

    Article Title: High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.389303

    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.
    Figure Legend Snippet: Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.

    Techniques Used: Suspension, Staining, CCK-8 Assay, Comparison, Cell Counting

    Effect of rTMS on NSC proliferation in the peri-infarct area. (A, B) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 5). (C, D) BrdU (red, Alexa Fluor® 555) and SOX-2 (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 3). Compared with the TMS group, rats in the Sham group exhibited less NSC proliferation. Proliferation of NSCs was significantly higher in the TMS (+) group compared with the TMS (–) group; shown by numbers of Nestin + /Ki67 + and SOX-2 + /BrdU + cells. Scale bars: 50 μm. Data are shown as mean ± SD. ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). BrdU: 5-Bromodeoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group.
    Figure Legend Snippet: Effect of rTMS on NSC proliferation in the peri-infarct area. (A, B) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 5). (C, D) BrdU (red, Alexa Fluor® 555) and SOX-2 (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 3). Compared with the TMS group, rats in the Sham group exhibited less NSC proliferation. Proliferation of NSCs was significantly higher in the TMS (+) group compared with the TMS (–) group; shown by numbers of Nestin + /Ki67 + and SOX-2 + /BrdU + cells. Scale bars: 50 μm. Data are shown as mean ± SD. ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). BrdU: 5-Bromodeoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group.

    Techniques Used: Comparison

    Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.
    Figure Legend Snippet: Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Comparison

    guinea pig anti mei s332  (Roche)


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    Roche guinea pig anti mei s332
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    alexa fluor 488 goat anti guinea pig igg fluorescein isothiocyanate isomer i fitc  (Santa Cruz Biotechnology)

     
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    Synaptic Systems guinea pig anti vgat
    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. <t>a-f</t> <t>CB1/VGLUT1/DAPI</t> immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l <t>CB1/VGAT/DAPI</t> immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
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    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f <t>CB1/VGLUT1/DAPI</t> immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests
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    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.
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    Roche guinea pig anti mei s332
    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.
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    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.
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    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.
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    Millipore guinea pig anti rabbit secondary
    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.
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    Image Search Results


    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice

    doi: 10.1007/s00018-023-05029-7

    Figure Lengend Snippet: Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Article Snippet: The following primary antibodies were used: guinea pig anti-VGluT1 (1:200; SYSY, cat. no. 135 304); rabbit anti-CB1 receptor antibody (anti-C-terminus 461–472, Abcam, ab23703, 1:300), guinea pig anti-VGAT (1:200, SYSY, cat. no. 131 004), goat anti-CB1 receptor antibody (anti-C-terminus 461–472, Abcam; ab40860; 1:300), rabbit anti-DGAL antibody (1:500) and goat anti-MAGL antibody (1:200; Abcam, cat. no. ab77398).

    Techniques: Immunolabeling, Comparison

    Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice

    doi: 10.1007/s00018-023-05029-7

    Figure Lengend Snippet: Distribution of CB1R immunoreactivity in glutamatergic and GABAergic inputs to the GCs in the DG of young and adult SynII KO mice. a-f CB1/VGLUT1/DAPI immunolabeling of the DG showing the increment of CB1/VGLUT1 colocalization in 1/2-month-old SynII KO in comparison to age-matched WT mice; this difference between the two genotypes is lost in 5/8-month-old mice. g–l CB1/VGAT/DAPI immunolabeling of the DG showing the reduction of CB1/VGAT colocalization in 5/8-month-old mice in comparison to age-matched mice of both genotypes. b1–i1 High-power fluorescent micrographs of orthogonal stacks are shown for each respective (b − i) areas; dotted lines and crosshairs are used to show 3D coordinates and define the area of interest. m Bar graph showing the percentage of overlap in the CB1R/VGLUT1 immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections per group in three mice; WT1/2 = 62.13 ± 1.30%, SynII KO1/2 = 77.43 ± 1.10%, WT5/8 = 71.33 ± 1.90%, SynII KO5/8 = 69.97 ± 1.7%. ***p < 0.001, two-way ANOVA (F1,116 = 19.65)/Tukey’s tests. n Bar graph showing the percentage of overlap in the CB1R/VGAT immunoreactivity areas in 1/2-month-old and 5/8-month-old mice. n = 30 sections in three mice; WT1/2 = 45.07 ± 1.4%, SynII KO1/2 = 47.47 ± 1.90%, WT5/8 = 23.27 ± 1.60%, SynII KO5/8 = 25.20 ± 1.60%., ****p < 0.0001, two-way ANOVA (F1.116 = 1.661)/Tukey’s tests

    Article Snippet: The following primary antibodies were used: guinea pig anti-VGluT1 (1:200; SYSY, cat. no. 135 304); rabbit anti-CB1 receptor antibody (anti-C-terminus 461–472, Abcam, ab23703, 1:300), guinea pig anti-VGAT (1:200, SYSY, cat. no. 131 004), goat anti-CB1 receptor antibody (anti-C-terminus 461–472, Abcam; ab40860; 1:300), rabbit anti-DGAL antibody (1:500) and goat anti-MAGL antibody (1:200; Abcam, cat. no. ab77398).

    Techniques: Immunolabeling, Comparison

    Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.

    Journal: Neural Regeneration Research

    Article Title: High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

    doi: 10.4103/1673-5374.389303

    Figure Lengend Snippet: Effect of different frequencies of rTMS on NSC proliferation. (A) Schematic diagram of the strategy to stimulate NSC proliferation. (B, C) Identification of NSCs. (B) NSCs under suspension (upper) and adherent (lower) culture were identified by SOX-2 (green, Alexa Fluor® 488), and Nestin (red, Alexa Fluor® 555) staining. Scale bars: 25 μm (upper) and 50 μm (lower). (C) NSCs were capable of differentiating into astrocytes (GFAP + , green, Alexa Fluor® 488), oligodendrocytes (CNPase + , red, Alexa Fluor® 555), and neurons (NeuN + , red, Alexa Fluor® 555). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue, DAPI). Scale bars: 50 μm. (D, E) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in CTRL, OGD, TMS (H), and TMS (L) groups. The percentage of Ki67 and Nestin double-positive cells in the sham and rTMS (H and L) groups were higher compared with the OGD group. Scale bars: 25 μm. (F) Cell viability by CCK-8 was increased in the TMS (H) group. Data are shown as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). CCK-8: Cell counting kit-8; CNpase: 2′,3′-cyclic nucleotide 3′ phosphodiesterase; CTRL: NSCs with no OGD group; GFAP: glial fibrillary acidic protein; ns: no significant; NSC: neural stem cell; OGD: oxygen-glucose deprivation; TMS: transcranial magnetic stimulation; TMS (–): NSCs with no TMS group; TMS (H): NSCs with 10 Hz high-frequency TMS group; TMS (L): NSCs with 1 Hz low-frequency TMS group.

    Article Snippet: Following primary antibody incubation, the cells were washed twice with PBS and then treated with secondary antibodies: Alexa Fluor 555/488 mouse anti-rabbit IgG (1:1000, Abcam, Cat# 4417S/4416S, RRID: AB_10895269/AB_10894182), Alexa Fluor 488 goat anti-guinea pig IgG-fluorescein isothiocyanate isomer I (FITC) (1:1000, Santa Cruz, Dallas, TX, USA, Cat# 374194, RRID: AB_10764638), Alexa Fluor 488/555 rabbit anti-mouse IgG (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 4408S/4409S, RRID: AB_142495/AB_1500655), or Alexa Fluor 555/488 rabbit anti-rat IgG (1:1000, Cell Signaling Technology, Cat# 4416S/4417S, RRID: AB_10894907/AB_10893616) for 1 hour at room temperature.

    Techniques: Suspension, Staining, CCK-8 Assay, Comparison, Cell Counting

    Effect of rTMS on NSC proliferation in the peri-infarct area. (A, B) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 5). (C, D) BrdU (red, Alexa Fluor® 555) and SOX-2 (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 3). Compared with the TMS group, rats in the Sham group exhibited less NSC proliferation. Proliferation of NSCs was significantly higher in the TMS (+) group compared with the TMS (–) group; shown by numbers of Nestin + /Ki67 + and SOX-2 + /BrdU + cells. Scale bars: 50 μm. Data are shown as mean ± SD. ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). BrdU: 5-Bromodeoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group.

    Journal: Neural Regeneration Research

    Article Title: High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

    doi: 10.4103/1673-5374.389303

    Figure Lengend Snippet: Effect of rTMS on NSC proliferation in the peri-infarct area. (A, B) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 5). (C, D) BrdU (red, Alexa Fluor® 555) and SOX-2 (green, Alexa Fluor® 488)-positive NSCs in Sham, TMS (–), and TMS (+) groups ( n = 3). Compared with the TMS group, rats in the Sham group exhibited less NSC proliferation. Proliferation of NSCs was significantly higher in the TMS (+) group compared with the TMS (–) group; shown by numbers of Nestin + /Ki67 + and SOX-2 + /BrdU + cells. Scale bars: 50 μm. Data are shown as mean ± SD. ** P < 0.01 (Brown-Forsythe and Welch analysis of variance tests followed by Dunnett's T3 multiple comparison test). BrdU: 5-Bromodeoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group.

    Article Snippet: Following primary antibody incubation, the cells were washed twice with PBS and then treated with secondary antibodies: Alexa Fluor 555/488 mouse anti-rabbit IgG (1:1000, Abcam, Cat# 4417S/4416S, RRID: AB_10895269/AB_10894182), Alexa Fluor 488 goat anti-guinea pig IgG-fluorescein isothiocyanate isomer I (FITC) (1:1000, Santa Cruz, Dallas, TX, USA, Cat# 374194, RRID: AB_10764638), Alexa Fluor 488/555 rabbit anti-mouse IgG (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 4408S/4409S, RRID: AB_142495/AB_1500655), or Alexa Fluor 555/488 rabbit anti-rat IgG (1:1000, Cell Signaling Technology, Cat# 4416S/4417S, RRID: AB_10894907/AB_10893616) for 1 hour at room temperature.

    Techniques: Comparison

    Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.

    Journal: Neural Regeneration Research

    Article Title: High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

    doi: 10.4103/1673-5374.389303

    Figure Lengend Snippet: Expression of phosphorylated-AKT (p-AKT), AKT, phosphorylated-GSK3β (p-GSK3β), GSK3β, and β-catenin in the nucleus of the ipsilateral hemisphere (excluding the infarct area). (A) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. Protein expression levels were normalized to GAPDH (total protein) or H3 (nuclear protein) ( n = 3). Expression of nuclear β-catenin, p-AKT, and p-GSK3β were higher in rats of the TMS (+) group compared with the TMS (–) group at 3, 7, 14, and 28 days. (B) Immunofluorescence of nuclear β-catenin (red, Alexa Fluor® 555) and Nestin (green)-positive cells in the TMS (+), TMS (–), and Sham groups ( n = 5). Nuclear β-catenin was upregulated in the TMS (+) group (analysis of variance [ANOVA]). Scale bars: 25 μm. (C) Ki67 (red, Alexa Fluor® 555) and Nestin (green, Alexa Fluor® 488)-positive cells in the TMS (–), TMS (H), and TMS + LY groups ( n = 5). More Ki67 + /Nestin + cells were detected in the TMS (H) group. Scale bars: 25 μm. (D) CCK8 analysis. Cell viability was decreased in the TMS (H) + LY group ( n = 5). (E) Western blot analysis of p-AKT, p-GSK3β, and β-catenin in the nucleus. GAPDH (total protein) or H3 (nuclear protein) were used as the internal control. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01 (A: Student's t -test; B, D, E: Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparison test). D3/7/14/28 TMS (–): rats with no TMS at 3/7/14/28 days group; D3/7/14/28 TMS (+): rats with 10 Hz TMS at 3/7/14/28 days group; GAPDH: glyceric acid phosphated hydrogenase; ns: no significant; NSC: neural stem cell; SHAM: sham-operation group; TMS: transcranial magnetic stimulation; TMS (–): no TMS group; TMS (+): high-frequency TMS with 10 Hz group; TMS(H): NSCs with 10 Hz high-frequency TMS group; TMS(H) + LY: NSCs with 10 Hz high-frequency TMS and LY294002 group.

    Article Snippet: Following primary antibody incubation, the cells were washed twice with PBS and then treated with secondary antibodies: Alexa Fluor 555/488 mouse anti-rabbit IgG (1:1000, Abcam, Cat# 4417S/4416S, RRID: AB_10895269/AB_10894182), Alexa Fluor 488 goat anti-guinea pig IgG-fluorescein isothiocyanate isomer I (FITC) (1:1000, Santa Cruz, Dallas, TX, USA, Cat# 374194, RRID: AB_10764638), Alexa Fluor 488/555 rabbit anti-mouse IgG (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 4408S/4409S, RRID: AB_142495/AB_1500655), or Alexa Fluor 555/488 rabbit anti-rat IgG (1:1000, Cell Signaling Technology, Cat# 4416S/4417S, RRID: AB_10894907/AB_10893616) for 1 hour at room temperature.

    Techniques: Expressing, Western Blot, Immunofluorescence, Comparison