Structured Review

Agilent technologies guinea pig antiinsulin
p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig <t>antiinsulin</t> (Ins; red ) or rabbit
Guinea Pig Antiinsulin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/guinea pig antiinsulin/product/Agilent technologies
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
guinea pig antiinsulin - by Bioz Stars, 2021-04
86/100 stars

Images

1) Product Images from "Diminished Pancreatic ?-Cell Mass in Securin-Null Mice Is Caused by ?-Cell Apoptosis and Senescence"

Article Title: Diminished Pancreatic ?-Cell Mass in Securin-Null Mice Is Caused by ?-Cell Apoptosis and Senescence

Journal: Endocrinology

doi: 10.1210/en.2008-0972

p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig antiinsulin (Ins; red ) or rabbit
Figure Legend Snippet: p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig antiinsulin (Ins; red ) or rabbit

Techniques Used: Mouse Assay, Staining

2) Product Images from "Diminished Pancreatic ?-Cell Mass in Securin-Null Mice Is Caused by ?-Cell Apoptosis and Senescence"

Article Title: Diminished Pancreatic ?-Cell Mass in Securin-Null Mice Is Caused by ?-Cell Apoptosis and Senescence

Journal: Endocrinology

doi: 10.1210/en.2008-0972

p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig antiinsulin (Ins; red ) or rabbit
Figure Legend Snippet: p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig antiinsulin (Ins; red ) or rabbit

Techniques Used: Mouse Assay, Staining

Related Articles

Staining:

Article Title: GLP-1 derivative liraglutide in rats with β-cell deficiencies: influence of metabolic state on β-cell mass dynamics
Article Snippet: The peroxidase activity was developed 5 min with 0.066% diaminobenzidine (DAB)+0.01% H2 O2 +2.5% NiSO4 to render BrdU containing nuclei black. .. Insulin was stained with guinea-pig antiinsulin (#651041, ICN) 1 : 400 in 7% rabbit serum 3% rat serum in TBS-T, peroxidase-coupled rabbit anti-guinea-pig Ig (P141, DAKO) 1 : 100, and developed with DAB for brown cytoplasm or with Vector Nova Red (Vector) according to the manufacturer, to stain the β -cell cytoplasm reddish brown. ..

Article Title: Transgenic Overexpression of the Transcription Factor Nkx6.1 in ?-Cells of Mice Does Not Increase ?-Cell Proliferation, ?-Cell Mass, or Improve Glucose Clearance
Article Snippet: Briefly, the entire pancreas was fixed overnight in 4% paraformaldehyde in PBS at 4 C and embedded in Tissue Tek O.C.T. embedding medium (Sakura Finetek, Tokyo, Japan) or paraffin for BrdU detection. .. Each pancreas was subsequently sectioned, and six representative sections were stained with the following antibodies: guinea pig antiinsulin (Dako, Carpinteria, CA) at 1:5000, rabbit antisomatostatin (Dako) at 1:2000, rabbit anti-Ki67 (Lab Vision, Fremont, CA) at 1:200, mouse anti-Nkx6.1 (Beta Cell Biology Consortium; clone 2023, National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases) at 1:500, rat anti-GFP (gift from C. Kioussi, Oregon State University, Corvallis, OR) at 1:1000, or mouse anti-BrdU (Chemicon, Temecula, CA) at 1:500. ..

Plasmid Preparation:

Article Title: GLP-1 derivative liraglutide in rats with β-cell deficiencies: influence of metabolic state on β-cell mass dynamics
Article Snippet: The peroxidase activity was developed 5 min with 0.066% diaminobenzidine (DAB)+0.01% H2 O2 +2.5% NiSO4 to render BrdU containing nuclei black. .. Insulin was stained with guinea-pig antiinsulin (#651041, ICN) 1 : 400 in 7% rabbit serum 3% rat serum in TBS-T, peroxidase-coupled rabbit anti-guinea-pig Ig (P141, DAKO) 1 : 100, and developed with DAB for brown cytoplasm or with Vector Nova Red (Vector) according to the manufacturer, to stain the β -cell cytoplasm reddish brown. ..

Blocking Assay:

Article Title: Synergy Between Gαz Deficiency and GLP-1 Analog Treatment in Preserving Functional β-Cell Mass in Experimental Diabetes
Article Snippet: Mouse antiactin (8H10D10), goat antirabbit horseradish peroxidase (HRP)-conjugated secondary antibody (7074), and horse antimouse HRP-conjugated secondary antibody (7076) were from Cell Signaling Technology. .. Guinea pig antiinsulin, rabbit anti-Ki67, antibody diluent with background reduction and serum-free blocking agent were from Dako. .. Citrate based antigen retrieval solution (H-3300), Cy3-coupled antiguinea pig secondary antibody, FITC-coupled antirabbit secondary antibody, and Vectashield Mounting Medium with DAPI were from Vector Laboratories.

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    Agilent technologies guinea pig antiinsulin
    p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig <t>antiinsulin</t> (Ins; red ) or rabbit
    Guinea Pig Antiinsulin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig antiinsulin/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig antiinsulin - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Agilent technologies guinea pig antiinsulin antibody
    Localization of VAMP.Aq. (A) Schematic map of VAMP.Aq. VAMP2 and aequorin cDNAs were fused via an HA1 epitope tag linker (Materials and methods) in order to localize mutated aequorin to the secretory vesicle lumen. (B) Confocal immunolocalization of VAMP.Aq. MIN6 cells were transfected with VAMP.Aq and stained with (a) mouse anti-HA1 monoclonal antibody (1:200) and (b) guinea pig <t>antiinsulin</t> antibody (1:150). (c) Extent of colocalization. (C) Immunoelectron microscopic localization of insulin (15-nm gold) or VAMP.Aq (anti-HA tag, 10-nm gold). Morphometric analysis of separate sections from 10 singly labeled cells revealed the following distribution of anti-HA gold particles: dense core vesicles, 36; ER, 2; Golgi apparatus, 0; plasma membrane, 16; endosomes, 19.
    Guinea Pig Antiinsulin Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig antiinsulin antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig antiinsulin antibody - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig antiinsulin (Ins; red ) or rabbit

    Journal: Endocrinology

    Article Title: Diminished Pancreatic ?-Cell Mass in Securin-Null Mice Is Caused by ?-Cell Apoptosis and Senescence

    doi: 10.1210/en.2008-0972

    Figure Lengend Snippet: p21 up-regulation in PTTG −/− mice. Paraffin sections of pancreas from WT or PTTG −/− mouse embryo at embryonic d 17 (ED17), newborn (NB), and 1- to 8-wk-old mice were stained with guinea pig antiinsulin (Ins; red ) or rabbit

    Article Snippet: For staining of islet hormones, 5-μm paraffin pancreatic sections were deparaffinized, rehydrated, and incubated with guinea pig antiinsulin at 1:200 to 1:2000 dilution, rabbit antiglucagon, antisomatostatin, or antipancreatic polypeptide (Dako, Carpinteria, CA) at 1:200 dilution, followed by rhodamine-labeled antiguinea pig or fluorescein isothiocyanate (FITC)-labeled antirabbit antiserum.

    Techniques: Mouse Assay, Staining

    Localization of VAMP.Aq. (A) Schematic map of VAMP.Aq. VAMP2 and aequorin cDNAs were fused via an HA1 epitope tag linker (Materials and methods) in order to localize mutated aequorin to the secretory vesicle lumen. (B) Confocal immunolocalization of VAMP.Aq. MIN6 cells were transfected with VAMP.Aq and stained with (a) mouse anti-HA1 monoclonal antibody (1:200) and (b) guinea pig antiinsulin antibody (1:150). (c) Extent of colocalization. (C) Immunoelectron microscopic localization of insulin (15-nm gold) or VAMP.Aq (anti-HA tag, 10-nm gold). Morphometric analysis of separate sections from 10 singly labeled cells revealed the following distribution of anti-HA gold particles: dense core vesicles, 36; ER, 2; Golgi apparatus, 0; plasma membrane, 16; endosomes, 19.

    Journal: The Journal of Cell Biology

    Article Title: Dense core secretory vesicles revealed as a dynamic Ca2+ store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera

    doi: 10.1083/jcb.200103145

    Figure Lengend Snippet: Localization of VAMP.Aq. (A) Schematic map of VAMP.Aq. VAMP2 and aequorin cDNAs were fused via an HA1 epitope tag linker (Materials and methods) in order to localize mutated aequorin to the secretory vesicle lumen. (B) Confocal immunolocalization of VAMP.Aq. MIN6 cells were transfected with VAMP.Aq and stained with (a) mouse anti-HA1 monoclonal antibody (1:200) and (b) guinea pig antiinsulin antibody (1:150). (c) Extent of colocalization. (C) Immunoelectron microscopic localization of insulin (15-nm gold) or VAMP.Aq (anti-HA tag, 10-nm gold). Morphometric analysis of separate sections from 10 singly labeled cells revealed the following distribution of anti-HA gold particles: dense core vesicles, 36; ER, 2; Golgi apparatus, 0; plasma membrane, 16; endosomes, 19.

    Article Snippet: For double labeling, anti-HA–labeled sections were incubated with 1% glutaraldehyde in 0.1 M Na2 PO4 , pH 7.0, followed by incubation with a guinea pig antiinsulin antibody (Dako) and 15-nm protein A-gold.

    Techniques: Transfection, Staining, Labeling