anti glua1 antibody produced in guinea pig  (Alomone Labs)


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    Alomone Labs anti glua1 antibody produced in guinea pig
    List of primary antibodies used
    Anti Glua1 Antibody Produced In Guinea Pig, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glua1 antibody produced in guinea pig/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glua1 antibody produced in guinea pig - by Bioz Stars, 2023-02
    93/100 stars

    Images

    1) Product Images from "Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain"

    Article Title: Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain

    Journal: Journal of Neuroscience Research

    doi: 10.1002/jnr.24967

    List of primary antibodies used
    Figure Legend Snippet: List of primary antibodies used

    Techniques Used: Concentration Assay, Produced, Recombinant, Purification, Derivative Assay, Transduction

    Antibodies used and amino acid conservation between species
    Figure Legend Snippet: Antibodies used and amino acid conservation between species

    Techniques Used:

    Molecular heterogeneity of astrocytes in the marmoset cerebellum. (A) Lower magnification images showing labeling for astrocytic proteins with respect to the molecular layer (ML), Purkinje cell layer (PCL), granule cell layer (GCL), and white matter (WM). Calbindin is used as a Purkinje cell marker. DAPI staining (blue) shows the nuclear staining of interneurons in the ML and granule cells in GCL. Scale bar: 100 μm. (B) Detailed images of ML and GCL stained with specific markers for Bergmann glia (BG) such as GluA1, Kirk4.1, and velate astrocytes (VA) such as AQP4. EAAT2 was expressed in both BGs and VAs with higher expression in VAs. Purkinje cell bodies are indicated with a white asterisk. Scale bar: 20 μm. bv, blood vessel
    Figure Legend Snippet: Molecular heterogeneity of astrocytes in the marmoset cerebellum. (A) Lower magnification images showing labeling for astrocytic proteins with respect to the molecular layer (ML), Purkinje cell layer (PCL), granule cell layer (GCL), and white matter (WM). Calbindin is used as a Purkinje cell marker. DAPI staining (blue) shows the nuclear staining of interneurons in the ML and granule cells in GCL. Scale bar: 100 μm. (B) Detailed images of ML and GCL stained with specific markers for Bergmann glia (BG) such as GluA1, Kirk4.1, and velate astrocytes (VA) such as AQP4. EAAT2 was expressed in both BGs and VAs with higher expression in VAs. Purkinje cell bodies are indicated with a white asterisk. Scale bar: 20 μm. bv, blood vessel

    Techniques Used: Labeling, Marker, Staining, Expressing

    anti glua1 antibody produced in guinea pig  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
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  • 93

    Structured Review

    Alomone Labs anti glua1 antibody produced in guinea pig
    List of primary antibodies used
    Anti Glua1 Antibody Produced In Guinea Pig, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glua1 antibody produced in guinea pig/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glua1 antibody produced in guinea pig - by Bioz Stars, 2023-02
    93/100 stars

    Images

    1) Product Images from "Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain"

    Article Title: Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain

    Journal: Journal of Neuroscience Research

    doi: 10.1002/jnr.24967

    List of primary antibodies used
    Figure Legend Snippet: List of primary antibodies used

    Techniques Used: Concentration Assay, Produced, Recombinant, Purification, Derivative Assay, Transduction

    Antibodies used and amino acid conservation between species
    Figure Legend Snippet: Antibodies used and amino acid conservation between species

    Techniques Used:

    Molecular heterogeneity of astrocytes in the marmoset cerebellum. (A) Lower magnification images showing labeling for astrocytic proteins with respect to the molecular layer (ML), Purkinje cell layer (PCL), granule cell layer (GCL), and white matter (WM). Calbindin is used as a Purkinje cell marker. DAPI staining (blue) shows the nuclear staining of interneurons in the ML and granule cells in GCL. Scale bar: 100 μm. (B) Detailed images of ML and GCL stained with specific markers for Bergmann glia (BG) such as GluA1, Kirk4.1, and velate astrocytes (VA) such as AQP4. EAAT2 was expressed in both BGs and VAs with higher expression in VAs. Purkinje cell bodies are indicated with a white asterisk. Scale bar: 20 μm. bv, blood vessel
    Figure Legend Snippet: Molecular heterogeneity of astrocytes in the marmoset cerebellum. (A) Lower magnification images showing labeling for astrocytic proteins with respect to the molecular layer (ML), Purkinje cell layer (PCL), granule cell layer (GCL), and white matter (WM). Calbindin is used as a Purkinje cell marker. DAPI staining (blue) shows the nuclear staining of interneurons in the ML and granule cells in GCL. Scale bar: 100 μm. (B) Detailed images of ML and GCL stained with specific markers for Bergmann glia (BG) such as GluA1, Kirk4.1, and velate astrocytes (VA) such as AQP4. EAAT2 was expressed in both BGs and VAs with higher expression in VAs. Purkinje cell bodies are indicated with a white asterisk. Scale bar: 20 μm. bv, blood vessel

    Techniques Used: Labeling, Marker, Staining, Expressing

    guinea pig anti glua1  (Alomone Labs)


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    Alomone Labs guinea pig anti glua1
    SYNPLA detects synapse formation and labels synapses. (A) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. (B) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. (C) PLA rolonies (white dots, Right) formed between postsynaptic cultured mouse hippocampal neurons expressing HA-NLGN + mCherry (red; Middle) and cocultured presynaptic neurons expressing myc-NRXN + GFP (green; Left). (Scale bar, 10 μm.) (D) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). ***P < 0.001, two-way ANOVA (see Methods); *P < 0.05, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) PLA reaction between endogenous <t>GluA1</t> and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP; see Fig. 2) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). (Scale bars, 10 μm, Left; 5 μm, Right.) Arrows represent dendritic spines labeled by presynaptic myc-NRXN, PSD-95, and PLA puncta. (F) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from E, Right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if nonzero pixels exist within 0.14 μm in both thresholded channels. For the indicated threshold, ∼95% of PLA but only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and postsynaptic markers. See SI Appendix, Fig. S3 and Materials and Methods for details.
    Guinea Pig Anti Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti glua1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti glua1 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "SYNPLA, a method to identify synapses displaying plasticity after learning"

    Article Title: SYNPLA, a method to identify synapses displaying plasticity after learning

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1919911117

    SYNPLA detects synapse formation and labels synapses. (A) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. (B) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. (C) PLA rolonies (white dots, Right) formed between postsynaptic cultured mouse hippocampal neurons expressing HA-NLGN + mCherry (red; Middle) and cocultured presynaptic neurons expressing myc-NRXN + GFP (green; Left). (Scale bar, 10 μm.) (D) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). ***P < 0.001, two-way ANOVA (see Methods); *P < 0.05, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) PLA reaction between endogenous GluA1 and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP; see Fig. 2) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). (Scale bars, 10 μm, Left; 5 μm, Right.) Arrows represent dendritic spines labeled by presynaptic myc-NRXN, PSD-95, and PLA puncta. (F) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from E, Right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if nonzero pixels exist within 0.14 μm in both thresholded channels. For the indicated threshold, ∼95% of PLA but only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and postsynaptic markers. See SI Appendix, Fig. S3 and Materials and Methods for details.
    Figure Legend Snippet: SYNPLA detects synapse formation and labels synapses. (A) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. (B) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. (C) PLA rolonies (white dots, Right) formed between postsynaptic cultured mouse hippocampal neurons expressing HA-NLGN + mCherry (red; Middle) and cocultured presynaptic neurons expressing myc-NRXN + GFP (green; Left). (Scale bar, 10 μm.) (D) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). ***P < 0.001, two-way ANOVA (see Methods); *P < 0.05, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) PLA reaction between endogenous GluA1 and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP; see Fig. 2) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). (Scale bars, 10 μm, Left; 5 μm, Right.) Arrows represent dendritic spines labeled by presynaptic myc-NRXN, PSD-95, and PLA puncta. (F) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from E, Right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if nonzero pixels exist within 0.14 μm in both thresholded channels. For the indicated threshold, ∼95% of PLA but only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and postsynaptic markers. See SI Appendix, Fig. S3 and Materials and Methods for details.

    Techniques Used: Binding Assay, Ligation, Amplification, Recombinant, Cell Culture, Expressing, In Vitro, Labeling

    SYNPLA detects synaptic potentiation in hippocampal primary cultures and organotypic slices. (A) Diagram of SYNPLA targeting presynaptic myc-NRXN and endogenous GluA1. cLTP induces mobilization of GluA1 from an extrasynaptic pool and insertion into the postsynaptic density, decreasing the distance between the targeted proteins and permitting PLA. (B) Representative images of SYNPLA reactions performed on myc-NRXN-expressing cultured rat hippocampal neurons at 14 d in vitro for the indicated conditions; PLA is shown in gray. (Scale bar, 10 μm.) (C) Quantification of a single SYNPLA experiment (as in F). The number of SYNPLA puncta detected in each field of view (circles) and the average (squares) ± SEM across all fields of view (12 per condition) are shown. ***P < 0.001, one-way ANOVA followed by the Tukey–Kramer post hoc test. (D) Quantification of six independent experiments (indicated by different colors); the average number of SYNPLA puncta across 10 to 12 fields of view for each experiment (circles, normalized to control [CTRL]); for each experiment, a significant difference was measured between CTRL and cLTP conditions (P values between 0.00004 and 0.02), and the average PLA signal across experiments (squares) ± SEM is shown. **P < 0.01, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) Diagram of SYNPLA in rat organotypic hippocampal slices. Sindbis virus expressing myc-NRXN is injected in the presynaptic CA3 region. SYNPLA between myc-NRXN and endogenous GluA1 is measured in the postsynaptic CA1 region. (F) Representative images of SYNPLA under the indicated conditions in region CA1 of organotypic slice cultures. (Scale bar, 10 μm.) (G) Quantification of SYNPLA puncta in organotypic slices from three independent experiments (indicated with different colors, four slices per condition [one circle per slice]); squares indicate average ± SEM across experiments. ***P < 0.001, paired t test.
    Figure Legend Snippet: SYNPLA detects synaptic potentiation in hippocampal primary cultures and organotypic slices. (A) Diagram of SYNPLA targeting presynaptic myc-NRXN and endogenous GluA1. cLTP induces mobilization of GluA1 from an extrasynaptic pool and insertion into the postsynaptic density, decreasing the distance between the targeted proteins and permitting PLA. (B) Representative images of SYNPLA reactions performed on myc-NRXN-expressing cultured rat hippocampal neurons at 14 d in vitro for the indicated conditions; PLA is shown in gray. (Scale bar, 10 μm.) (C) Quantification of a single SYNPLA experiment (as in F). The number of SYNPLA puncta detected in each field of view (circles) and the average (squares) ± SEM across all fields of view (12 per condition) are shown. ***P < 0.001, one-way ANOVA followed by the Tukey–Kramer post hoc test. (D) Quantification of six independent experiments (indicated by different colors); the average number of SYNPLA puncta across 10 to 12 fields of view for each experiment (circles, normalized to control [CTRL]); for each experiment, a significant difference was measured between CTRL and cLTP conditions (P values between 0.00004 and 0.02), and the average PLA signal across experiments (squares) ± SEM is shown. **P < 0.01, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) Diagram of SYNPLA in rat organotypic hippocampal slices. Sindbis virus expressing myc-NRXN is injected in the presynaptic CA3 region. SYNPLA between myc-NRXN and endogenous GluA1 is measured in the postsynaptic CA1 region. (F) Representative images of SYNPLA under the indicated conditions in region CA1 of organotypic slice cultures. (Scale bar, 10 μm.) (G) Quantification of SYNPLA puncta in organotypic slices from three independent experiments (indicated with different colors, four slices per condition [one circle per slice]); squares indicate average ± SEM across experiments. ***P < 0.001, paired t test.

    Techniques Used: Expressing, Cell Culture, In Vitro, Injection

    polyclonal guinea pig antibody against glua1  (Alomone Labs)


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    Alomone Labs polyclonal guinea pig antibody against glua1
    Patient-derived LG1 IgG causes a decrease of total cell surface and synaptic AMPAR clusters in the hippocampus. (A) 3D projection and analysis of the density of total clusters of <t>GluA1</t> AMPAR and PSD95, and synaptic clusters of AMPAR (defined as AMPAR clusters that co-localized with PSD95) in one of the CA3 subregions indicated in Fig. 3A, ‘Analysis’. Merged images (A, top and bottom left; GluA1 green, and PSD95 red) were post-processed and used to calculate the density of the indicated clusters (density = spots/μm3). Scale bar = 2 μm. (B–D) Quantification of the density of total (B) and synaptic (C) GluA1 AMPAR clusters, and PSD95 clusters (D) in a pooled analysis of hippocampal subregions (CA1, CA3, dentate gyrus) in animals treated with patient-derived LGI1 IgG (red) or control IgG (black) on the indicated days. Mean density of clusters in control IgG treated animals was defined as 100%. Data are presented as mean ± SEM. For each time point five animals infused with patient-derived IgG and five with control IgG were examined. Significance of treatment effect was assessed by two-way ANOVA with an alpha-error of 0.05 (asterisks) and post hoc testing with Sidak-Holm adjustment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
    Polyclonal Guinea Pig Antibody Against Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal guinea pig antibody against glua1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal guinea pig antibody against glua1 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "LGI1 antibodies alter K v 1.1 and AMPA receptors changing synaptic excitability, plasticity and memory"

    Article Title: LGI1 antibodies alter K v 1.1 and AMPA receptors changing synaptic excitability, plasticity and memory

    Journal: Brain

    doi: 10.1093/brain/awy253

    Patient-derived LG1 IgG causes a decrease of total cell surface and synaptic AMPAR clusters in the hippocampus. (A) 3D projection and analysis of the density of total clusters of GluA1 AMPAR and PSD95, and synaptic clusters of AMPAR (defined as AMPAR clusters that co-localized with PSD95) in one of the CA3 subregions indicated in Fig. 3A, ‘Analysis’. Merged images (A, top and bottom left; GluA1 green, and PSD95 red) were post-processed and used to calculate the density of the indicated clusters (density = spots/μm3). Scale bar = 2 μm. (B–D) Quantification of the density of total (B) and synaptic (C) GluA1 AMPAR clusters, and PSD95 clusters (D) in a pooled analysis of hippocampal subregions (CA1, CA3, dentate gyrus) in animals treated with patient-derived LGI1 IgG (red) or control IgG (black) on the indicated days. Mean density of clusters in control IgG treated animals was defined as 100%. Data are presented as mean ± SEM. For each time point five animals infused with patient-derived IgG and five with control IgG were examined. Significance of treatment effect was assessed by two-way ANOVA with an alpha-error of 0.05 (asterisks) and post hoc testing with Sidak-Holm adjustment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
    Figure Legend Snippet: Patient-derived LG1 IgG causes a decrease of total cell surface and synaptic AMPAR clusters in the hippocampus. (A) 3D projection and analysis of the density of total clusters of GluA1 AMPAR and PSD95, and synaptic clusters of AMPAR (defined as AMPAR clusters that co-localized with PSD95) in one of the CA3 subregions indicated in Fig. 3A, ‘Analysis’. Merged images (A, top and bottom left; GluA1 green, and PSD95 red) were post-processed and used to calculate the density of the indicated clusters (density = spots/μm3). Scale bar = 2 μm. (B–D) Quantification of the density of total (B) and synaptic (C) GluA1 AMPAR clusters, and PSD95 clusters (D) in a pooled analysis of hippocampal subregions (CA1, CA3, dentate gyrus) in animals treated with patient-derived LGI1 IgG (red) or control IgG (black) on the indicated days. Mean density of clusters in control IgG treated animals was defined as 100%. Data are presented as mean ± SEM. For each time point five animals infused with patient-derived IgG and five with control IgG were examined. Significance of treatment effect was assessed by two-way ANOVA with an alpha-error of 0.05 (asterisks) and post hoc testing with Sidak-Holm adjustment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Techniques Used: Derivative Assay

    guinea pig anti glua1  (Alomone Labs)


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    Alomone Labs guinea pig anti glua1
    Distribution of AMPA receptor subunits in the olfactory nerve layer (ONL). (A) Laminar organization of the olfactory bulb. Nuclei were stained with Hoechst (5 μM). (B) The ONL, glomerular layer (GL), external plexiform layer (EPL) and granule cell layer (GCL) are clearly distinguishable. Scale bar: 200 μm. (C) Distribution of PLP-Cre-dependent tdTomato expression in the ONL and GL. Olfactory ensheathing cells (OECs) are located in the ONL, whereas tdTomato-expressing oligodendrocytes are located in the GL. Nuclei are stained with Dapi (5 μM; blue). The assumed border between the ONL and GL is indicated by the dotted line. Glomeruli are marked by asterisks. Scale bar: 150 μm. (D) Magnified view from (C). OECs are located in the ONL (arrowhead) and oligodendrocytes (arrow) in the GL visualized by PLP-Cre-dependent tdTomato expression (red). Scale bar: 50 μm. (E) Distribution of <t>GluA1</t> immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (F) Magnified view from (E). GluA1 immunostaining (green) colocalize with tdTomato-expressing OECs (red) in the ONL (arrowheads). Scale bar: 25 μm. (G) Distribution of GluA2 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). GluA2 is widely distributed in the ONL, as well as in the GL. Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (H) Magnified view from (G). GluA2 immunoreactivity colocalizes with tdTomato-expressing OECs (red) in the ONL (arrowheads). Scale bar: 25 μm. (I) Distribution of GluA4 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (J) Magnified view from (I). GluA4 immunoreactivity (green) is sparsely colocalized with tdTomato-positive OEC somata (red), but is localized adjacent to OEC somata (arrowheads). Scale bar: 25 μm.
    Guinea Pig Anti Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti glua1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti glua1 - by Bioz Stars, 2023-02
    93/100 stars

    Images

    1) Product Images from "AMPA Receptor-Mediated Ca 2+ Transients in Mouse Olfactory Ensheathing Cells"

    Article Title: AMPA Receptor-Mediated Ca 2+ Transients in Mouse Olfactory Ensheathing Cells

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2019.00451

    Distribution of AMPA receptor subunits in the olfactory nerve layer (ONL). (A) Laminar organization of the olfactory bulb. Nuclei were stained with Hoechst (5 μM). (B) The ONL, glomerular layer (GL), external plexiform layer (EPL) and granule cell layer (GCL) are clearly distinguishable. Scale bar: 200 μm. (C) Distribution of PLP-Cre-dependent tdTomato expression in the ONL and GL. Olfactory ensheathing cells (OECs) are located in the ONL, whereas tdTomato-expressing oligodendrocytes are located in the GL. Nuclei are stained with Dapi (5 μM; blue). The assumed border between the ONL and GL is indicated by the dotted line. Glomeruli are marked by asterisks. Scale bar: 150 μm. (D) Magnified view from (C). OECs are located in the ONL (arrowhead) and oligodendrocytes (arrow) in the GL visualized by PLP-Cre-dependent tdTomato expression (red). Scale bar: 50 μm. (E) Distribution of GluA1 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (F) Magnified view from (E). GluA1 immunostaining (green) colocalize with tdTomato-expressing OECs (red) in the ONL (arrowheads). Scale bar: 25 μm. (G) Distribution of GluA2 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). GluA2 is widely distributed in the ONL, as well as in the GL. Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (H) Magnified view from (G). GluA2 immunoreactivity colocalizes with tdTomato-expressing OECs (red) in the ONL (arrowheads). Scale bar: 25 μm. (I) Distribution of GluA4 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (J) Magnified view from (I). GluA4 immunoreactivity (green) is sparsely colocalized with tdTomato-positive OEC somata (red), but is localized adjacent to OEC somata (arrowheads). Scale bar: 25 μm.
    Figure Legend Snippet: Distribution of AMPA receptor subunits in the olfactory nerve layer (ONL). (A) Laminar organization of the olfactory bulb. Nuclei were stained with Hoechst (5 μM). (B) The ONL, glomerular layer (GL), external plexiform layer (EPL) and granule cell layer (GCL) are clearly distinguishable. Scale bar: 200 μm. (C) Distribution of PLP-Cre-dependent tdTomato expression in the ONL and GL. Olfactory ensheathing cells (OECs) are located in the ONL, whereas tdTomato-expressing oligodendrocytes are located in the GL. Nuclei are stained with Dapi (5 μM; blue). The assumed border between the ONL and GL is indicated by the dotted line. Glomeruli are marked by asterisks. Scale bar: 150 μm. (D) Magnified view from (C). OECs are located in the ONL (arrowhead) and oligodendrocytes (arrow) in the GL visualized by PLP-Cre-dependent tdTomato expression (red). Scale bar: 50 μm. (E) Distribution of GluA1 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (F) Magnified view from (E). GluA1 immunostaining (green) colocalize with tdTomato-expressing OECs (red) in the ONL (arrowheads). Scale bar: 25 μm. (G) Distribution of GluA2 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). GluA2 is widely distributed in the ONL, as well as in the GL. Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (H) Magnified view from (G). GluA2 immunoreactivity colocalizes with tdTomato-expressing OECs (red) in the ONL (arrowheads). Scale bar: 25 μm. (I) Distribution of GluA4 immunoreactivity (green) in the ONL of PLP-Cre ERT2 × tdTomato fl/fl mice (red). Nuclei are stained with Dapi (blue). Glomeruli are marked by asterisks. Scale bar: 50 μm. (J) Magnified view from (I). GluA4 immunoreactivity (green) is sparsely colocalized with tdTomato-positive OEC somata (red), but is localized adjacent to OEC somata (arrowheads). Scale bar: 25 μm.

    Techniques Used: Staining, Expressing, Immunostaining

    guinea pig anti glua1  (Alomone Labs)


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    Structured Review

    Alomone Labs guinea pig anti glua1
    a ) Diagram of SYNPLA targeting presynaptic myc-NRXN and. cLTP induces mobilization of <t>GluA1</t> from an extrasynaptic pool and insertion into the post-synaptic density, decreasing the distance between the targeted proteins and permitting PLA. b ) Representative images of SYNPLA reactions performed on myc-NRXN expressing cultured rat hippocampal neurons at 14 days in vitro for the indicated conditions; PLA shown in gray. Scale bar: 10 μm. c ) Quantification of a single SYNPLA experiment (as in f ). The number of SYNPLA puncta detected in each field of view (round) and the average (square) ± SEM across all fields of view are shown; *** p<0.001, 1-way ANOVA followed by Tukey-Kramer post-hoc test. d ) Quantification of six independent experiments (indicated by different colors); the average number of SYNPLA puncta across 10-12 fields of view for each experiment (circles, normalized to CTRL) and the average PLA signal across experiments (square) ± SEM are shown; ** p<0.01, 1-way ANOVA (with Tukey-Kramer post-hoc test). e ) Diagram of SYNPLA in rat organotypic hippocampal slices. Sindbis virus expressing myc-NRXN is injected in the presynaptic CA3 region SYNPLA between myc-NRXN and endogenous GluA1 is measured in the postsynaptic CA1 region. f ) Representative images of SYNPLA under the indicated conditions in region CA1 of organotypic slice cultures. Scale bar: 10 μm. g ) Quantification of SYNPLA puncta in organotypic slices, from three independent experiments (indicated with different colors, 4 slices per condition (one circle per slice)); squares indicate average ± SEM across experiments; *** p<0.001, paired t-test.
    Guinea Pig Anti Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "SYNPLA: A synapse-specific method for identifying learning-induced synaptic plasticity loci"

    Article Title: SYNPLA: A synapse-specific method for identifying learning-induced synaptic plasticity loci

    Journal: bioRxiv

    doi: 10.1101/473314

    a ) Diagram of SYNPLA targeting presynaptic myc-NRXN and. cLTP induces mobilization of GluA1 from an extrasynaptic pool and insertion into the post-synaptic density, decreasing the distance between the targeted proteins and permitting PLA. b ) Representative images of SYNPLA reactions performed on myc-NRXN expressing cultured rat hippocampal neurons at 14 days in vitro for the indicated conditions; PLA shown in gray. Scale bar: 10 μm. c ) Quantification of a single SYNPLA experiment (as in f ). The number of SYNPLA puncta detected in each field of view (round) and the average (square) ± SEM across all fields of view are shown; *** p<0.001, 1-way ANOVA followed by Tukey-Kramer post-hoc test. d ) Quantification of six independent experiments (indicated by different colors); the average number of SYNPLA puncta across 10-12 fields of view for each experiment (circles, normalized to CTRL) and the average PLA signal across experiments (square) ± SEM are shown; ** p<0.01, 1-way ANOVA (with Tukey-Kramer post-hoc test). e ) Diagram of SYNPLA in rat organotypic hippocampal slices. Sindbis virus expressing myc-NRXN is injected in the presynaptic CA3 region SYNPLA between myc-NRXN and endogenous GluA1 is measured in the postsynaptic CA1 region. f ) Representative images of SYNPLA under the indicated conditions in region CA1 of organotypic slice cultures. Scale bar: 10 μm. g ) Quantification of SYNPLA puncta in organotypic slices, from three independent experiments (indicated with different colors, 4 slices per condition (one circle per slice)); squares indicate average ± SEM across experiments; *** p<0.001, paired t-test.
    Figure Legend Snippet: a ) Diagram of SYNPLA targeting presynaptic myc-NRXN and. cLTP induces mobilization of GluA1 from an extrasynaptic pool and insertion into the post-synaptic density, decreasing the distance between the targeted proteins and permitting PLA. b ) Representative images of SYNPLA reactions performed on myc-NRXN expressing cultured rat hippocampal neurons at 14 days in vitro for the indicated conditions; PLA shown in gray. Scale bar: 10 μm. c ) Quantification of a single SYNPLA experiment (as in f ). The number of SYNPLA puncta detected in each field of view (round) and the average (square) ± SEM across all fields of view are shown; *** p<0.001, 1-way ANOVA followed by Tukey-Kramer post-hoc test. d ) Quantification of six independent experiments (indicated by different colors); the average number of SYNPLA puncta across 10-12 fields of view for each experiment (circles, normalized to CTRL) and the average PLA signal across experiments (square) ± SEM are shown; ** p<0.01, 1-way ANOVA (with Tukey-Kramer post-hoc test). e ) Diagram of SYNPLA in rat organotypic hippocampal slices. Sindbis virus expressing myc-NRXN is injected in the presynaptic CA3 region SYNPLA between myc-NRXN and endogenous GluA1 is measured in the postsynaptic CA1 region. f ) Representative images of SYNPLA under the indicated conditions in region CA1 of organotypic slice cultures. Scale bar: 10 μm. g ) Quantification of SYNPLA puncta in organotypic slices, from three independent experiments (indicated with different colors, 4 slices per condition (one circle per slice)); squares indicate average ± SEM across experiments; *** p<0.001, paired t-test.

    Techniques Used: Expressing, Cell Culture, In Vitro, Injection

    a ) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. b ) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. c ) PLA rolonies (white dots, right) formed between postsynaptic cultured mouse hippocampal neuron expressing HA-NLGN + mCherry (red; middle) and co-cultured presynaptic neurons expressing myc-NRXN + GFP (green; left). Scale bar: 10 μm. d ) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). *** p<0.001, 2-way ANOVA (see Methods); * p<0.05, 1-way ANOVA (with Tukey-Kramer post-hoc test). e ) PLA reaction between endogenous GluA1 and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP, see ) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). Scale bars: 10 μm (left); 5 μm (right). f ) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from e , right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if non-zero pixels exist within 0.14 μm in both thresholded channels. For indicated threshold, ∼95% of PLA, while only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and post-synaptic markers. See Supplementary Fig. 3 and Methods for details.
    Figure Legend Snippet: a ) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. b ) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. c ) PLA rolonies (white dots, right) formed between postsynaptic cultured mouse hippocampal neuron expressing HA-NLGN + mCherry (red; middle) and co-cultured presynaptic neurons expressing myc-NRXN + GFP (green; left). Scale bar: 10 μm. d ) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). *** p<0.001, 2-way ANOVA (see Methods); * p<0.05, 1-way ANOVA (with Tukey-Kramer post-hoc test). e ) PLA reaction between endogenous GluA1 and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP, see ) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). Scale bars: 10 μm (left); 5 μm (right). f ) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from e , right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if non-zero pixels exist within 0.14 μm in both thresholded channels. For indicated threshold, ∼95% of PLA, while only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and post-synaptic markers. See Supplementary Fig. 3 and Methods for details.

    Techniques Used: Binding Assay, Ligation, Amplification, Recombinant, Cell Culture, Expressing, In Vitro

    guinea pig anti glua1  (Alomone Labs)


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    Alomone Labs guinea pig anti glua1
    (A) Cell surface <t>GluA1</t> immunostaining of wild type and Sema3F−/− cortical cultures (14 DIV) treated for 48 hrs with TTX, bicuculline or control media.
    Guinea Pig Anti Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Neuropilin-2/PlexinA3 Receptors Associate with GluA1 and Mediate Sema3F-dependent Homeostatic Scaling in Cortical Neurons"

    Article Title: Neuropilin-2/PlexinA3 Receptors Associate with GluA1 and Mediate Sema3F-dependent Homeostatic Scaling in Cortical Neurons

    Journal: Neuron

    doi: 10.1016/j.neuron.2017.10.029

    (A) Cell surface GluA1 immunostaining of wild type and Sema3F−/− cortical cultures (14 DIV) treated for 48 hrs with TTX, bicuculline or control media.
    Figure Legend Snippet: (A) Cell surface GluA1 immunostaining of wild type and Sema3F−/− cortical cultures (14 DIV) treated for 48 hrs with TTX, bicuculline or control media.

    Techniques Used: Immunostaining

    (A) Regulation of the interaction between HA-GluA1 and FLAG-Npn-2 in HEK293T cells by Sema3F. HEK293T cells transfected with HA-GluA1, FLAG-Npn-2, and Myc-PlexA3 constructs were treated with AP-Sema3F (5 nM) for the indicated times. Npn-2 was co-immunoprecipitated with GluA1 from the transfected cell lysates using an HA antibody.
    Figure Legend Snippet: (A) Regulation of the interaction between HA-GluA1 and FLAG-Npn-2 in HEK293T cells by Sema3F. HEK293T cells transfected with HA-GluA1, FLAG-Npn-2, and Myc-PlexA3 constructs were treated with AP-Sema3F (5 nM) for the indicated times. Npn-2 was co-immunoprecipitated with GluA1 from the transfected cell lysates using an HA antibody.

    Techniques Used: Transfection, Construct, Immunoprecipitation

    (A) HEK293T cells were transfected with constructs expressing FLAG-Npn-2 and HA-GluA1, with or without PlexA3, and then treated with Sema3F for 30 min. HA-GluA1 was immunoprecipitated with an HA antibody and coimmunoprecipitated FLAG-Npn-2 was detected using a FLAG antibody.
    Figure Legend Snippet: (A) HEK293T cells were transfected with constructs expressing FLAG-Npn-2 and HA-GluA1, with or without PlexA3, and then treated with Sema3F for 30 min. HA-GluA1 was immunoprecipitated with an HA antibody and coimmunoprecipitated FLAG-Npn-2 was detected using a FLAG antibody.

    Techniques Used: Transfection, Construct, Expressing, Immunoprecipitation

    guinea pig anti glua1  (Alomone Labs)


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    Alomone Labs guinea pig anti glua1
    (A) Cell surface <t>GluA1</t> immunostaining of wild type and Sema3F−/− cortical cultures (14 DIV) treated for 48 hrs with TTX, bicuculline or control media.
    Guinea Pig Anti Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Neuropilin-2/PlexinA3 Receptors Associate with GluA1 and Mediate Sema3F-dependent Homeostatic Scaling in Cortical Neurons"

    Article Title: Neuropilin-2/PlexinA3 Receptors Associate with GluA1 and Mediate Sema3F-dependent Homeostatic Scaling in Cortical Neurons

    Journal: Neuron

    doi: 10.1016/j.neuron.2017.10.029

    (A) Cell surface GluA1 immunostaining of wild type and Sema3F−/− cortical cultures (14 DIV) treated for 48 hrs with TTX, bicuculline or control media.
    Figure Legend Snippet: (A) Cell surface GluA1 immunostaining of wild type and Sema3F−/− cortical cultures (14 DIV) treated for 48 hrs with TTX, bicuculline or control media.

    Techniques Used: Immunostaining

    (A) Regulation of the interaction between HA-GluA1 and FLAG-Npn-2 in HEK293T cells by Sema3F. HEK293T cells transfected with HA-GluA1, FLAG-Npn-2, and Myc-PlexA3 constructs were treated with AP-Sema3F (5 nM) for the indicated times. Npn-2 was co-immunoprecipitated with GluA1 from the transfected cell lysates using an HA antibody.
    Figure Legend Snippet: (A) Regulation of the interaction between HA-GluA1 and FLAG-Npn-2 in HEK293T cells by Sema3F. HEK293T cells transfected with HA-GluA1, FLAG-Npn-2, and Myc-PlexA3 constructs were treated with AP-Sema3F (5 nM) for the indicated times. Npn-2 was co-immunoprecipitated with GluA1 from the transfected cell lysates using an HA antibody.

    Techniques Used: Transfection, Construct, Immunoprecipitation

    (A) HEK293T cells were transfected with constructs expressing FLAG-Npn-2 and HA-GluA1, with or without PlexA3, and then treated with Sema3F for 30 min. HA-GluA1 was immunoprecipitated with an HA antibody and coimmunoprecipitated FLAG-Npn-2 was detected using a FLAG antibody.
    Figure Legend Snippet: (A) HEK293T cells were transfected with constructs expressing FLAG-Npn-2 and HA-GluA1, with or without PlexA3, and then treated with Sema3F for 30 min. HA-GluA1 was immunoprecipitated with an HA antibody and coimmunoprecipitated FLAG-Npn-2 was detected using a FLAG antibody.

    Techniques Used: Transfection, Construct, Expressing, Immunoprecipitation

    antibodies anti glua1  (Alomone Labs)


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    Alomone Labs antibodies anti glua1
    ( a ) GluA2 immunoreactivity (green) was detected in the external plexiform layer (EPL) and in cell bodies surrounding glomeruli. Glomeruli are indicated by asterisks. Moderate GluA2 immunoreactivity was also found in astrocytes highlighted by GFAP immunoreactivity (red), as indicated by yellow pixels in the merged image. Arrows point to astrocyte structures that were colabeled with GluA immunoreactivity. Nuclei were stained with Hoechst 33342 (blue). ( b ) <t>GluA1</t> and GFAP colocalization. ( c ) GluA4 and GFAP colocalization. Scale bars: 20 μm.
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    Images

    1) Product Images from "Ca 2+ -permeable AMPA receptors in mouse olfactory bulb astrocytes"

    Article Title: Ca 2+ -permeable AMPA receptors in mouse olfactory bulb astrocytes

    Journal: Scientific Reports

    doi: 10.1038/srep44817

    ( a ) GluA2 immunoreactivity (green) was detected in the external plexiform layer (EPL) and in cell bodies surrounding glomeruli. Glomeruli are indicated by asterisks. Moderate GluA2 immunoreactivity was also found in astrocytes highlighted by GFAP immunoreactivity (red), as indicated by yellow pixels in the merged image. Arrows point to astrocyte structures that were colabeled with GluA immunoreactivity. Nuclei were stained with Hoechst 33342 (blue). ( b ) GluA1 and GFAP colocalization. ( c ) GluA4 and GFAP colocalization. Scale bars: 20 μm.
    Figure Legend Snippet: ( a ) GluA2 immunoreactivity (green) was detected in the external plexiform layer (EPL) and in cell bodies surrounding glomeruli. Glomeruli are indicated by asterisks. Moderate GluA2 immunoreactivity was also found in astrocytes highlighted by GFAP immunoreactivity (red), as indicated by yellow pixels in the merged image. Arrows point to astrocyte structures that were colabeled with GluA immunoreactivity. Nuclei were stained with Hoechst 33342 (blue). ( b ) GluA1 and GFAP colocalization. ( c ) GluA4 and GFAP colocalization. Scale bars: 20 μm.

    Techniques Used: Staining

    guinea pig glua1 antibody  (Alomone Labs)


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    Alomone Labs guinea pig glua1 antibody
    Guinea Pig Glua1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti glua1 antibody produced in guinea pig
    List of primary antibodies used
    Anti Glua1 Antibody Produced In Guinea Pig, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs guinea pig anti glua1
    SYNPLA detects synapse formation and labels synapses. (A) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. (B) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. (C) PLA rolonies (white dots, Right) formed between postsynaptic cultured mouse hippocampal neurons expressing HA-NLGN + mCherry (red; Middle) and cocultured presynaptic neurons expressing myc-NRXN + GFP (green; Left). (Scale bar, 10 μm.) (D) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). ***P < 0.001, two-way ANOVA (see Methods); *P < 0.05, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) PLA reaction between endogenous <t>GluA1</t> and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP; see Fig. 2) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). (Scale bars, 10 μm, Left; 5 μm, Right.) Arrows represent dendritic spines labeled by presynaptic myc-NRXN, PSD-95, and PLA puncta. (F) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from E, Right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if nonzero pixels exist within 0.14 μm in both thresholded channels. For the indicated threshold, ∼95% of PLA but only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and postsynaptic markers. See SI Appendix, Fig. S3 and Materials and Methods for details.
    Guinea Pig Anti Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs polyclonal guinea pig antibody against glua1
    Patient-derived LG1 IgG causes a decrease of total cell surface and synaptic AMPAR clusters in the hippocampus. (A) 3D projection and analysis of the density of total clusters of <t>GluA1</t> AMPAR and PSD95, and synaptic clusters of AMPAR (defined as AMPAR clusters that co-localized with PSD95) in one of the CA3 subregions indicated in Fig. 3A, ‘Analysis’. Merged images (A, top and bottom left; GluA1 green, and PSD95 red) were post-processed and used to calculate the density of the indicated clusters (density = spots/μm3). Scale bar = 2 μm. (B–D) Quantification of the density of total (B) and synaptic (C) GluA1 AMPAR clusters, and PSD95 clusters (D) in a pooled analysis of hippocampal subregions (CA1, CA3, dentate gyrus) in animals treated with patient-derived LGI1 IgG (red) or control IgG (black) on the indicated days. Mean density of clusters in control IgG treated animals was defined as 100%. Data are presented as mean ± SEM. For each time point five animals infused with patient-derived IgG and five with control IgG were examined. Significance of treatment effect was assessed by two-way ANOVA with an alpha-error of 0.05 (asterisks) and post hoc testing with Sidak-Holm adjustment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
    Polyclonal Guinea Pig Antibody Against Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs antibodies anti glua1
    ( a ) GluA2 immunoreactivity (green) was detected in the external plexiform layer (EPL) and in cell bodies surrounding glomeruli. Glomeruli are indicated by asterisks. Moderate GluA2 immunoreactivity was also found in astrocytes highlighted by GFAP immunoreactivity (red), as indicated by yellow pixels in the merged image. Arrows point to astrocyte structures that were colabeled with GluA immunoreactivity. Nuclei were stained with Hoechst 33342 (blue). ( b ) <t>GluA1</t> and GFAP colocalization. ( c ) GluA4 and GFAP colocalization. Scale bars: 20 μm.
    Antibodies Anti Glua1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs guinea pig glua1 antibody
    ( a ) GluA2 immunoreactivity (green) was detected in the external plexiform layer (EPL) and in cell bodies surrounding glomeruli. Glomeruli are indicated by asterisks. Moderate GluA2 immunoreactivity was also found in astrocytes highlighted by GFAP immunoreactivity (red), as indicated by yellow pixels in the merged image. Arrows point to astrocyte structures that were colabeled with GluA immunoreactivity. Nuclei were stained with Hoechst 33342 (blue). ( b ) <t>GluA1</t> and GFAP colocalization. ( c ) GluA4 and GFAP colocalization. Scale bars: 20 μm.
    Guinea Pig Glua1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig glua1 antibody/product/Alomone Labs
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    Image Search Results


    List of primary antibodies used

    Journal: Journal of Neuroscience Research

    Article Title: Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain

    doi: 10.1002/jnr.24967

    Figure Lengend Snippet: List of primary antibodies used

    Article Snippet: Anti‐GluA1 antibody produced in guinea pig , Peptide corresponding to AA 271‐285 of rat GluR1 , Alomone lab, # AGP‐009, Polyclonal antibody, RRID:AB_2340961 , 3.2 μg/ml/1:250.

    Techniques: Concentration Assay, Produced, Recombinant, Purification, Derivative Assay, Transduction

    Antibodies used and amino acid conservation between species

    Journal: Journal of Neuroscience Research

    Article Title: Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain

    doi: 10.1002/jnr.24967

    Figure Lengend Snippet: Antibodies used and amino acid conservation between species

    Article Snippet: Anti‐GluA1 antibody produced in guinea pig , Peptide corresponding to AA 271‐285 of rat GluR1 , Alomone lab, # AGP‐009, Polyclonal antibody, RRID:AB_2340961 , 3.2 μg/ml/1:250.

    Techniques:

    Molecular heterogeneity of astrocytes in the marmoset cerebellum. (A) Lower magnification images showing labeling for astrocytic proteins with respect to the molecular layer (ML), Purkinje cell layer (PCL), granule cell layer (GCL), and white matter (WM). Calbindin is used as a Purkinje cell marker. DAPI staining (blue) shows the nuclear staining of interneurons in the ML and granule cells in GCL. Scale bar: 100 μm. (B) Detailed images of ML and GCL stained with specific markers for Bergmann glia (BG) such as GluA1, Kirk4.1, and velate astrocytes (VA) such as AQP4. EAAT2 was expressed in both BGs and VAs with higher expression in VAs. Purkinje cell bodies are indicated with a white asterisk. Scale bar: 20 μm. bv, blood vessel

    Journal: Journal of Neuroscience Research

    Article Title: Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain

    doi: 10.1002/jnr.24967

    Figure Lengend Snippet: Molecular heterogeneity of astrocytes in the marmoset cerebellum. (A) Lower magnification images showing labeling for astrocytic proteins with respect to the molecular layer (ML), Purkinje cell layer (PCL), granule cell layer (GCL), and white matter (WM). Calbindin is used as a Purkinje cell marker. DAPI staining (blue) shows the nuclear staining of interneurons in the ML and granule cells in GCL. Scale bar: 100 μm. (B) Detailed images of ML and GCL stained with specific markers for Bergmann glia (BG) such as GluA1, Kirk4.1, and velate astrocytes (VA) such as AQP4. EAAT2 was expressed in both BGs and VAs with higher expression in VAs. Purkinje cell bodies are indicated with a white asterisk. Scale bar: 20 μm. bv, blood vessel

    Article Snippet: Anti‐GluA1 antibody produced in guinea pig , Peptide corresponding to AA 271‐285 of rat GluR1 , Alomone lab, # AGP‐009, Polyclonal antibody, RRID:AB_2340961 , 3.2 μg/ml/1:250.

    Techniques: Labeling, Marker, Staining, Expressing

    SYNPLA detects synapse formation and labels synapses. (A) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. (B) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. (C) PLA rolonies (white dots, Right) formed between postsynaptic cultured mouse hippocampal neurons expressing HA-NLGN + mCherry (red; Middle) and cocultured presynaptic neurons expressing myc-NRXN + GFP (green; Left). (Scale bar, 10 μm.) (D) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). ***P < 0.001, two-way ANOVA (see Methods); *P < 0.05, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) PLA reaction between endogenous GluA1 and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP; see Fig. 2) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). (Scale bars, 10 μm, Left; 5 μm, Right.) Arrows represent dendritic spines labeled by presynaptic myc-NRXN, PSD-95, and PLA puncta. (F) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from E, Right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if nonzero pixels exist within 0.14 μm in both thresholded channels. For the indicated threshold, ∼95% of PLA but only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and postsynaptic markers. See SI Appendix, Fig. S3 and Materials and Methods for details.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: SYNPLA, a method to identify synapses displaying plasticity after learning

    doi: 10.1073/pnas.1919911117

    Figure Lengend Snippet: SYNPLA detects synapse formation and labels synapses. (A) Diagram of a general PLA reaction. Note that A and B must be sufficiently close to permit complementary binding and ligation of ABo1 and ABo2 and subsequent amplification to make a rolony. (B) Diagram of PLA targeting recombinant presynaptic myc-NRXN and postsynaptic HA-NLGN. (C) PLA rolonies (white dots, Right) formed between postsynaptic cultured mouse hippocampal neurons expressing HA-NLGN + mCherry (red; Middle) and cocultured presynaptic neurons expressing myc-NRXN + GFP (green; Left). (Scale bar, 10 μm.) (D) PLA puncta in each sample (circles) and average (squares) ± SEM for indicated expression and days in vitro (color indicates data acquired on the same day). ***P < 0.001, two-way ANOVA (see Methods); *P < 0.05, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) PLA reaction between endogenous GluA1 and recombinant myc in cultured rat hippocampal neurons (exposed to cLTP; see Fig. 2) expressing myc-NRXN and cytosolic fluorescent protein (green), subsequently immunostained for endogenous PSD-95 (red). (Scale bars, 10 μm, Left; 5 μm, Right.) Arrows represent dendritic spines labeled by presynaptic myc-NRXN, PSD-95, and PLA puncta. (F) PLA puncta localize to synapses. In PSD-95 and myc-NRXN channels (from E, Right), pixels were set to zero (masked) for values below a progressively increasing threshold (x axis). Puncta (PLA or randomly placed) display colocalization if nonzero pixels exist within 0.14 μm in both thresholded channels. For the indicated threshold, ∼95% of PLA but only ∼50% of randomly placed (mean ± SEM of 30 placements) puncta colocalized with pre- and postsynaptic markers. See SI Appendix, Fig. S3 and Materials and Methods for details.

    Article Snippet: For SI Appendix , Fig. S2 different primary antibodies were used as negative controls: guinea pig anti-GluA1 (AGP-009, Alomone Labs), rabbit anti-cFos (Cell Signaling Technology, #2250); all were at 1:100 dilution.

    Techniques: Binding Assay, Ligation, Amplification, Recombinant, Cell Culture, Expressing, In Vitro, Labeling

    SYNPLA detects synaptic potentiation in hippocampal primary cultures and organotypic slices. (A) Diagram of SYNPLA targeting presynaptic myc-NRXN and endogenous GluA1. cLTP induces mobilization of GluA1 from an extrasynaptic pool and insertion into the postsynaptic density, decreasing the distance between the targeted proteins and permitting PLA. (B) Representative images of SYNPLA reactions performed on myc-NRXN-expressing cultured rat hippocampal neurons at 14 d in vitro for the indicated conditions; PLA is shown in gray. (Scale bar, 10 μm.) (C) Quantification of a single SYNPLA experiment (as in F). The number of SYNPLA puncta detected in each field of view (circles) and the average (squares) ± SEM across all fields of view (12 per condition) are shown. ***P < 0.001, one-way ANOVA followed by the Tukey–Kramer post hoc test. (D) Quantification of six independent experiments (indicated by different colors); the average number of SYNPLA puncta across 10 to 12 fields of view for each experiment (circles, normalized to control [CTRL]); for each experiment, a significant difference was measured between CTRL and cLTP conditions (P values between 0.00004 and 0.02), and the average PLA signal across experiments (squares) ± SEM is shown. **P < 0.01, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) Diagram of SYNPLA in rat organotypic hippocampal slices. Sindbis virus expressing myc-NRXN is injected in the presynaptic CA3 region. SYNPLA between myc-NRXN and endogenous GluA1 is measured in the postsynaptic CA1 region. (F) Representative images of SYNPLA under the indicated conditions in region CA1 of organotypic slice cultures. (Scale bar, 10 μm.) (G) Quantification of SYNPLA puncta in organotypic slices from three independent experiments (indicated with different colors, four slices per condition [one circle per slice]); squares indicate average ± SEM across experiments. ***P < 0.001, paired t test.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: SYNPLA, a method to identify synapses displaying plasticity after learning

    doi: 10.1073/pnas.1919911117

    Figure Lengend Snippet: SYNPLA detects synaptic potentiation in hippocampal primary cultures and organotypic slices. (A) Diagram of SYNPLA targeting presynaptic myc-NRXN and endogenous GluA1. cLTP induces mobilization of GluA1 from an extrasynaptic pool and insertion into the postsynaptic density, decreasing the distance between the targeted proteins and permitting PLA. (B) Representative images of SYNPLA reactions performed on myc-NRXN-expressing cultured rat hippocampal neurons at 14 d in vitro for the indicated conditions; PLA is shown in gray. (Scale bar, 10 μm.) (C) Quantification of a single SYNPLA experiment (as in F). The number of SYNPLA puncta detected in each field of view (circles) and the average (squares) ± SEM across all fields of view (12 per condition) are shown. ***P < 0.001, one-way ANOVA followed by the Tukey–Kramer post hoc test. (D) Quantification of six independent experiments (indicated by different colors); the average number of SYNPLA puncta across 10 to 12 fields of view for each experiment (circles, normalized to control [CTRL]); for each experiment, a significant difference was measured between CTRL and cLTP conditions (P values between 0.00004 and 0.02), and the average PLA signal across experiments (squares) ± SEM is shown. **P < 0.01, one-way ANOVA (with the Tukey–Kramer post hoc test). (E) Diagram of SYNPLA in rat organotypic hippocampal slices. Sindbis virus expressing myc-NRXN is injected in the presynaptic CA3 region. SYNPLA between myc-NRXN and endogenous GluA1 is measured in the postsynaptic CA1 region. (F) Representative images of SYNPLA under the indicated conditions in region CA1 of organotypic slice cultures. (Scale bar, 10 μm.) (G) Quantification of SYNPLA puncta in organotypic slices from three independent experiments (indicated with different colors, four slices per condition [one circle per slice]); squares indicate average ± SEM across experiments. ***P < 0.001, paired t test.

    Article Snippet: For SI Appendix , Fig. S2 different primary antibodies were used as negative controls: guinea pig anti-GluA1 (AGP-009, Alomone Labs), rabbit anti-cFos (Cell Signaling Technology, #2250); all were at 1:100 dilution.

    Techniques: Expressing, Cell Culture, In Vitro, Injection

    Patient-derived LG1 IgG causes a decrease of total cell surface and synaptic AMPAR clusters in the hippocampus. (A) 3D projection and analysis of the density of total clusters of GluA1 AMPAR and PSD95, and synaptic clusters of AMPAR (defined as AMPAR clusters that co-localized with PSD95) in one of the CA3 subregions indicated in Fig. 3A, ‘Analysis’. Merged images (A, top and bottom left; GluA1 green, and PSD95 red) were post-processed and used to calculate the density of the indicated clusters (density = spots/μm3). Scale bar = 2 μm. (B–D) Quantification of the density of total (B) and synaptic (C) GluA1 AMPAR clusters, and PSD95 clusters (D) in a pooled analysis of hippocampal subregions (CA1, CA3, dentate gyrus) in animals treated with patient-derived LGI1 IgG (red) or control IgG (black) on the indicated days. Mean density of clusters in control IgG treated animals was defined as 100%. Data are presented as mean ± SEM. For each time point five animals infused with patient-derived IgG and five with control IgG were examined. Significance of treatment effect was assessed by two-way ANOVA with an alpha-error of 0.05 (asterisks) and post hoc testing with Sidak-Holm adjustment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Journal: Brain

    Article Title: LGI1 antibodies alter K v 1.1 and AMPA receptors changing synaptic excitability, plasticity and memory

    doi: 10.1093/brain/awy253

    Figure Lengend Snippet: Patient-derived LG1 IgG causes a decrease of total cell surface and synaptic AMPAR clusters in the hippocampus. (A) 3D projection and analysis of the density of total clusters of GluA1 AMPAR and PSD95, and synaptic clusters of AMPAR (defined as AMPAR clusters that co-localized with PSD95) in one of the CA3 subregions indicated in Fig. 3A, ‘Analysis’. Merged images (A, top and bottom left; GluA1 green, and PSD95 red) were post-processed and used to calculate the density of the indicated clusters (density = spots/μm3). Scale bar = 2 μm. (B–D) Quantification of the density of total (B) and synaptic (C) GluA1 AMPAR clusters, and PSD95 clusters (D) in a pooled analysis of hippocampal subregions (CA1, CA3, dentate gyrus) in animals treated with patient-derived LGI1 IgG (red) or control IgG (black) on the indicated days. Mean density of clusters in control IgG treated animals was defined as 100%. Data are presented as mean ± SEM. For each time point five animals infused with patient-derived IgG and five with control IgG were examined. Significance of treatment effect was assessed by two-way ANOVA with an alpha-error of 0.05 (asterisks) and post hoc testing with Sidak-Holm adjustment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Article Snippet: To determine whether the postsynaptic levels of AMPAR were affected, brain sections were incubated with a polyclonal guinea pig antibody against GluA1 (1:200, #AGP009, Alomone) for 2 h at room temperature, washed in PBS containing 0.3% Triton, and incubated with a rabbit polyclonal antibody against the postsynaptic marker PSD95 (1:250, #18258, Abcam) overnight at 4°C.

    Techniques: Derivative Assay

    ( a ) GluA2 immunoreactivity (green) was detected in the external plexiform layer (EPL) and in cell bodies surrounding glomeruli. Glomeruli are indicated by asterisks. Moderate GluA2 immunoreactivity was also found in astrocytes highlighted by GFAP immunoreactivity (red), as indicated by yellow pixels in the merged image. Arrows point to astrocyte structures that were colabeled with GluA immunoreactivity. Nuclei were stained with Hoechst 33342 (blue). ( b ) GluA1 and GFAP colocalization. ( c ) GluA4 and GFAP colocalization. Scale bars: 20 μm.

    Journal: Scientific Reports

    Article Title: Ca 2+ -permeable AMPA receptors in mouse olfactory bulb astrocytes

    doi: 10.1038/srep44817

    Figure Lengend Snippet: ( a ) GluA2 immunoreactivity (green) was detected in the external plexiform layer (EPL) and in cell bodies surrounding glomeruli. Glomeruli are indicated by asterisks. Moderate GluA2 immunoreactivity was also found in astrocytes highlighted by GFAP immunoreactivity (red), as indicated by yellow pixels in the merged image. Arrows point to astrocyte structures that were colabeled with GluA immunoreactivity. Nuclei were stained with Hoechst 33342 (blue). ( b ) GluA1 and GFAP colocalization. ( c ) GluA4 and GFAP colocalization. Scale bars: 20 μm.

    Article Snippet: Slices were cut with a vibratome (VT1000, Leica) and incubated with the primary antibodies anti-GluA1 (guinea pig; 1:200; Alomone Labs), anti-GluA2 (rabbit; 1:200; Millipore, Darmstadt, Germany), anti-GluA4 (rabbit; 1:200; Millipore), anti-GFAP (rabbit, 1:000, Dako, Hamburg, Germany), anti-GFAP (chicken; 1:500; Abcam) and anti-GFP (chicken, 1:500, SySy, Göttingen, Germany).

    Techniques: Staining