guinea pig anti bdnf antibody  (Alomone Labs)

Journal: Scientific Reports

Article Title: Telomerase increasing compound protects hippocampal neurons from amyloid beta toxicity by enhancing the expression of neurotrophins and plasticity related genes

doi: 10.1038/s41598-019-54741-7

Figure Lengend Snippet: AGS increases BDNF but not NGF gene expression in both Aβ treated and untreated cultures. Cultures were treated for 36 hr. with AGS-499 (200 nM) alone, Aβ (5 µM) alone, and Aβ (5 µM) in combination with AGS-499 (200 nM) or with its vehicle DMSO. AGS or DMSO treatments were renewed after 24 hr. Expression levels were quantified using RT-PCR and normalized to the untreated control (Control). ( a ) Relative BDNF gene expression in cultures (Mean ± SEM, n = 5 independent experiments), ANOVA test ***p

Article Snippet: The brain slices (5 μΜ) derived from vehicle (DMSO) and AGS-499 treated mice were subjected to immunofluorescence analysis using as first antibody: Guinea pig anti BDNF-antibody (1:100, # AGP-021, alomono labs, Israel) or anti-beta catenin antibody (1:100, β-Catenin (6B3) Rabbit mAb #9582, Cell Signaling technology, Danvers, MA, USA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal:

Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

doi: 10.1111/j.1471-4159.2009.05990.x

Figure Lengend Snippet: Time course of Smad 1/5/8 phosphorylation following addition of BMP7 ± BDNF

Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

Techniques:

Journal:

Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

doi: 10.1111/j.1471-4159.2009.05990.x

Figure Lengend Snippet: Hypoglycemia increases the enhancement of nuclear and cytoplasmic P-Smad levels produced by both BMP7 alone and a BMP7 + BDNF combination

Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

Techniques: Produced

Journal:

Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

doi: 10.1111/j.1471-4159.2009.05990.x

Figure Lengend Snippet: BDNF-induced increases in phosphorylated Akt and Erk are not modified by BMP7 or a CK2 inhibitor, TBB

Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

Techniques: Modification

Journal:

Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

doi: 10.1111/j.1471-4159.2009.05990.x

Figure Lengend Snippet: Localization of catalytic subunits of CK2 in septal neurons and activation of CK2 by BDNF and BMP7

Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

Techniques: Activation Assay

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Circadian clock genes and respiratory neuroplasticity genes oscillate in the phrenic motor system

doi: 10.1152/ajpregu.00010.2020

Figure Lengend Snippet: Immunofluorescence ( A ) and semiquantification ( B ) of BDNF protein expression within phrenic motor neurons retrogradely labeled with cholera toxin beta (CtB) in the ventral C3–C5 spinal cord from rats harvested at the end of the rest phase (ZT11; 5 PM) compared with the end of the active phase (ZT23; 5 AM). BDNF, green; CtB, magenta; a.u., arbitrary units. Scale bar = 100 µm. Values represent means ± SD of n = 11 rats/group). * P

Article Snippet: Sections were incubated overnight at 4°C with the following primary antibodies: BMAL1 (Cell Signaling) or BDNF (Alomone) and CtB (Millipore).

Techniques: Immunofluorescence, Expressing, Labeling, CtB Assay

Journal: bioRxiv

Article Title: p75 Neurotrophin Receptor Regulates the Timing of the Maturation of Cortical Parvalbumin Cell Connectivity and Promotes Ocular Dominance Plasticity in Adult Visual Cortex

doi: 10.1101/392159

Figure Lengend Snippet: proBNDF-mediated p75NTR activation in cortical PV cells reduces their perisomatic boutons and restores ocular dominance plasticity in adult visual cortex in vivo . (a) Experimental approach. (b) The number of immunolabeled PV-positive puncta (green) surrounding NeuN-positive neuronal somata (red) is reduced in the binocular visual cortex ipsilateral to the minipump releasing mut-proBDNF (Ipsi) compared to the contralateral cortex (Contra) in the same animal. On the other hand, the number of PV-positive puncta per NeuN-positive profile in the ipsilateral cortex of PV-CRE; p75 flx/flx mice is similar to that observed in the contralateral, untreated cortex. (c) Low (c1) and high (c2) magnification of PNN (red, WFA staining) enwrapping PV cells (green) show a dramatic reduction in both PNN density and intensity in the visual cortex infused with mut-proBFNF. This effects is abolished in PV-CRE; p75 flx/flx mice. Scale bar, c1: 100µm; b, c2: 10µm. (d) Quantification of the mean number of PV-positive puncta per NeuN-positive profile in ipsilateral compared to contralateral cortex. Ipsi/Contra ratio is obtained for each animal, and then averaged between different animals. Mean Ipsi/Contra ratio is significantly reduced in Mut-proBDNF infused p75 Ctrl but not in PV-CRE; p75 flx/flx mice (t-test, p

Article Snippet: In addition, we tested the following anti-proBDNF antibodies: chicken anti-proBDNF (Millipore, AB9042), rabbit-anti-proBDNF (Alomone Labs, ANT-006) and guinea-pig-anti-proBDNF (Alomone Lab, AGP-032).

Techniques: Activation Assay, In Vivo, Immunolabeling, Mouse Assay, Staining, T-Test

Journal: bioRxiv

Article Title: p75 Neurotrophin Receptor Regulates the Timing of the Maturation of Cortical Parvalbumin Cell Connectivity and Promotes Ocular Dominance Plasticity in Adult Visual Cortex

doi: 10.1101/392159

Figure Lengend Snippet: mut-proBDNF can destabilize PV cell innervation even after it has reached maturity. (a) Control PV cell (a1, Ctrl, green) at EP32 with exuberant innervation field characterized by extensive branching contacting the majority of potential targets, dense boutons along axons (a2), and terminal branches with prominent and clustered boutons (a3; arrowheads) around pyramidal cell somata (NeuN immunostaining, blue). (b) PV cell treated with wt-proBDNF from EP26-32 shows overall similar axon size (b1), percentage of potentially targeted neurons (B2) and perisomatic innervations (b3) as control, untreated PV cells. (c) PV cell treated with mut-proBDNF from EP26-32 shows a drastic reduction both in percentage of innervated cells (c2) and perisomatic innervation (c3). Stars indicate pyramidal cells somata that are not innervated. Scale bar, a1-c1: 50µm; a2-c2: 10µm; a3-c3: 5µm. (d) Perisomatic bouton density (e) terminal branching and (f) percentage of innervated cells of the three experimental groups. One-way Anova, post hoc Tukey test, p

Article Snippet: In addition, we tested the following anti-proBDNF antibodies: chicken anti-proBDNF (Millipore, AB9042), rabbit-anti-proBDNF (Alomone Labs, ANT-006) and guinea-pig-anti-proBDNF (Alomone Lab, AGP-032).

Techniques: Immunostaining