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    Name:
    Guanosine 5 Triphosphate Disodium Salt
    Description:
    Guanosine 5 Triphosphate Disodium Salt is a phosphoryl donor in protein synthesis and in signal transduction
    Catalog Number:
    SC-295030
    Price:
    None
    Category:
    Chemicals Other Chemicals Nucleic Acids Nucleotides and Nucleosides Guanosine 5 Triphosphate Disodium Salt
    Applications:
    Guanosine 5′-Triphosphate, Disodium Salt is a phosphoryl donor in protein synthesis and signal transduction
    Molecular Weight:
    567.14
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    Structured Review

    Santa Cruz Biotechnology gtp
    Guanosine 5 Triphosphate Disodium Salt is a phosphoryl donor in protein synthesis and in signal transduction
    https://www.bioz.com/result/gtp/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gtp - by Bioz Stars, 2021-07
    92/100 stars

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    Related Articles

    Modification:

    Article Title: DLGAP1 directs megakaryocytic growth and differentiation in an MPL dependent manner in hematopoietic cells
    Article Snippet: .. When indicated, culture medium was supplemented with one of the following reagents: 10 pg/ml AP20187 - a dimerizer of proteins containing an F36 V domain, which is a modified FKBP-binding domain [ ] (ARIAD Pharmaceuticals, Cambridge, MA), 1 μM Imatinib (Novartis, Basel, Switzerland), 10 pg/ml GMCSF, 50 ng/ml PEG-rhMGDF, 400 mg/ml Geneticin (Invitrogen, Carlsbad, CA), 50 μM AG490 (Cayman Chemical Co., Ann Arbor, MI [Cayman]), 2.5 μM SU6656 (Cayman), 20 μM U0126 (Cell Signaling Technology, Boston, MA), 100 ng/ml Nocodazole (SCBT), 40 nM PMA (SCBT), 200 μM GTP (SCBT), 50 μM Olomoucine (SCBT), 50 μM iso-Olomoucine (SCBT) and 4.5 μM or 9 μM RO3306 (SCBT). ..

    Western Blot:

    Article Title: A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells
    Article Snippet: U2OS Cell Model System The generation and characterisation of U2OS cells stably expressing Epac1 and Epac2 and the protocols to monitor Epac and PKA activities have been described previously [ ]. .. In brief, after 20 min of stimulation, cells were lysed and Rap•GTP levels determined by precipitating GTP-bound Rap and Western blot analysis with a α-Rap antibody (Santa Cruz Biotechnology, USA). .. To determine PKA activity, total cell lysates were subjected to Western blotting with a monoclonal α-VASP antibody (BD Transduction Laboratories, USA).

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  • 93
    Santa Cruz Biotechnology grα
    Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The <t>GRα</t> and GRβ cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors αβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.
    Grα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grα/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Santa Cruz Biotechnology gtp levels
    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. <t>Rap•GTP</t> was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.
    Gtp Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gtp levels/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
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    96
    Santa Cruz Biotechnology polyclonal rabbit anti grα antibodies
    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. <t>Rap•GTP</t> was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.
    Polyclonal Rabbit Anti Grα Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology gr p 20 antibodies
    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. <t>Rap•GTP</t> was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.
    Gr P 20 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The GRα and GRβ cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors αβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The GRα and GRβ cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors αβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Western Blot, Expressing

    Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients . GRα and GRβ cell expression were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on ICU admission (first day in the ICU) and on the day of hospital discharge, which was considered as the control situation (free of sepsis). The quantification of the western blot analysis of (A) GRα and (B) GRβ is shown. The values were normalized to β actin expression and are expressed as percentage values of the control (GR expression on the day of hospital discharge). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). (C) Representative western blot analysis of GRαGRβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients . GRα and GRβ cell expression were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on ICU admission (first day in the ICU) and on the day of hospital discharge, which was considered as the control situation (free of sepsis). The quantification of the western blot analysis of (A) GRα and (B) GRβ is shown. The values were normalized to β actin expression and are expressed as percentage values of the control (GR expression on the day of hospital discharge). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). (C) Representative western blot analysis of GRαGRβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Western Blot, Expressing

    Glucocorticoid receptor isoform expression on the first and third day in the intensive care unit for the survivors and non-survivors of sepsis . GRα and β cell expression were evaluated on the first and third days in the ICU by western blotting of peripheral blood mononuclear cells from septic patients who survived or did not survive the septic event. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in the ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GRα, β and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Glucocorticoid receptor isoform expression on the first and third day in the intensive care unit for the survivors and non-survivors of sepsis . GRα and β cell expression were evaluated on the first and third days in the ICU by western blotting of peripheral blood mononuclear cells from septic patients who survived or did not survive the septic event. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in the ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GRα, β and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Expressing, Western Blot

    Effect of human septic serum on glucocorticoid receptor isoform expression in different cell lines . CEM, Raji and K562 cell lines were cultured in the presence of the patients' septic serum and serum obtained from the control group at 10% final concentration in the culture medium. After 48 h in culture, the GR protein expression was analyzed by western blotting with specific antibodies for GRα and β isoforms. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown ( n = 9). The values were normalized to β actin expression and are expressed as percentage values of the control (GR cell expression cultured in the presence of control serum). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). Representative western blot analysis of GRα, β and β actin are shown for each cell line. CS, control serum; GR, glucocorticoid receptor; SS, septic serum.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Effect of human septic serum on glucocorticoid receptor isoform expression in different cell lines . CEM, Raji and K562 cell lines were cultured in the presence of the patients' septic serum and serum obtained from the control group at 10% final concentration in the culture medium. After 48 h in culture, the GR protein expression was analyzed by western blotting with specific antibodies for GRα and β isoforms. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown ( n = 9). The values were normalized to β actin expression and are expressed as percentage values of the control (GR cell expression cultured in the presence of control serum). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). Representative western blot analysis of GRα, β and β actin are shown for each cell line. CS, control serum; GR, glucocorticoid receptor; SS, septic serum.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Expressing, Cell Culture, Concentration Assay, Western Blot

    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. Rap•GTP was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.

    Journal: Cells

    Article Title: A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells

    doi: 10.3390/cells8121589

    Figure Lengend Snippet: Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. Rap•GTP was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.

    Article Snippet: In brief, after 20 min of stimulation, cells were lysed and Rap•GTP levels determined by precipitating GTP-bound Rap and Western blot analysis with a α-Rap antibody (Santa Cruz Biotechnology, USA).

    Techniques: Activation Assay, Stable Transfection, Expressing, Electrophoretic Mobility Shift Assay