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    Name:
    GTP 14564
    Description:
    GTP 14564 is a cell permeable tricyclic benzofurano indazolo compound which functions as a specific inhibitor of class III receptor tyrosine kinases Studies show that GTP 14564 can be used to track the signaling pathways activated by FLT3 and ITD FLT3 in Ba F3 cells Furthermore GTP 14564 can suppress the FLT3 internal tandem duplication ITD cells to the same extent as FLT3 siRNA Studies show that if combined with 17 AAG GTP 14564 can reduce the levels of p FLT3 and p STAT5 and can amplify apoptosis and G0 G1 arrest in FLT3 ITD cells GTP 14564 is an inhibitor of c Fms CSF 1R c Kit Flt 3 Flk 2 and PDGFR beta
    Catalog Number:
    SC-203062
    Price:
    None
    Category:
    Chemicals Protein Interacting Inhibitors Activators Substrates Protein Inhibitors c Fms CSF 1R Inhibitors GTP 14564
    Applications:
    GTP 14564 is a specific inhibitor of class III receptor tyrosine kinases
    Purity:
    >98%
    Molecular Weight:
    234.26
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    Structured Review

    Santa Cruz Biotechnology gtp
    MoImd4 is required for the purine metabolic pathway in Magnaporthe oryzae and exogenous xanthosine monophosphate <t>(XMP)</t> suppresses the defects of the S345AC347A mutant in vegetative growth and virulence. (A) The de novo <t>GTP/ATP</t> biosynthesis pathway of M. oryzae . (B, C) Intracellular levels of XMP/GTP in mycelia of the indicated strains by high‐performance liquid chromatography (HPLC). Experiments were repeated three times with similar results. The error bars indicate the standard deviations of three replicates. Different letters indicate statistically significant differences (Duncan’s new multiple range test, P
    GTP 14564 is a cell permeable tricyclic benzofurano indazolo compound which functions as a specific inhibitor of class III receptor tyrosine kinases Studies show that GTP 14564 can be used to track the signaling pathways activated by FLT3 and ITD FLT3 in Ba F3 cells Furthermore GTP 14564 can suppress the FLT3 internal tandem duplication ITD cells to the same extent as FLT3 siRNA Studies show that if combined with 17 AAG GTP 14564 can reduce the levels of p FLT3 and p STAT5 and can amplify apoptosis and G0 G1 arrest in FLT3 ITD cells GTP 14564 is an inhibitor of c Fms CSF 1R c Kit Flt 3 Flk 2 and PDGFR beta
    https://www.bioz.com/result/gtp/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gtp - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "MoImd4 mediates crosstalk between MoPdeH‐cAMP signalling and purine metabolism to govern growth and pathogenicity in Magnaporthe oryzae"

    Article Title: MoImd4 mediates crosstalk between MoPdeH‐cAMP signalling and purine metabolism to govern growth and pathogenicity in Magnaporthe oryzae

    Journal: Molecular Plant Pathology

    doi: 10.1111/mpp.12770

    MoImd4 is required for the purine metabolic pathway in Magnaporthe oryzae and exogenous xanthosine monophosphate (XMP) suppresses the defects of the S345AC347A mutant in vegetative growth and virulence. (A) The de novo GTP/ATP biosynthesis pathway of M. oryzae . (B, C) Intracellular levels of XMP/GTP in mycelia of the indicated strains by high‐performance liquid chromatography (HPLC). Experiments were repeated three times with similar results. The error bars indicate the standard deviations of three replicates. Different letters indicate statistically significant differences (Duncan’s new multiple range test, P
    Figure Legend Snippet: MoImd4 is required for the purine metabolic pathway in Magnaporthe oryzae and exogenous xanthosine monophosphate (XMP) suppresses the defects of the S345AC347A mutant in vegetative growth and virulence. (A) The de novo GTP/ATP biosynthesis pathway of M. oryzae . (B, C) Intracellular levels of XMP/GTP in mycelia of the indicated strains by high‐performance liquid chromatography (HPLC). Experiments were repeated three times with similar results. The error bars indicate the standard deviations of three replicates. Different letters indicate statistically significant differences (Duncan’s new multiple range test, P

    Techniques Used: Mutagenesis, High Performance Liquid Chromatography

    Related Articles

    Flow Cytometry:

    Article Title: MoImd4 mediates crosstalk between MoPdeH‐cAMP signalling and purine metabolism to govern growth and pathogenicity in Magnaporthe oryzae
    Article Snippet: .. The peak flow of the standard of XMP (Alading, X113495) was at ~6.4 min, of the standard of GTP (Santa Cruz, sc‐203062, USA) was at ~2.6 min, and of the standard of cAMP (Meilune, MB3159) was at ~13.8 min. ..

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    Santa Cruz Biotechnology gtp cyclohydrolase
    A : mRNA levels for inducible nitric oxide (NO) synthase (iNOS) and <t>GTP</t> <t>cyclohydrolase</t> (GTPCH) in cytokine-stimulated cardiac myocytes for 12 h. Similar findings were observed at 18 h (not shown). Ctrl, control. B : Western blots for protein expression
    Gtp Cyclohydrolase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gtp cyclohydrolase/product/Santa Cruz Biotechnology
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    86
    Santa Cruz Biotechnology rhoa gtp
    Inhibition of <t>RhoA</t> activity by C3 toxin expression strongly affects DNA damage response, including global and specific DNA repair mechanisms in HeLa cells, following γ -radiation. (a) Dendritic morphology of HeLa cells (HeLa + C3 images) associated with decreased <t>RhoA-GTP</t> levels (on the right), 24 h after transfection with a plasmid for C3 toxin expression. Images on the right are insets from those on the left, 200x. (b) Estimates of DNA damage and repair efficiency (by olive tail moment, or OTM, measurements from comet assays) in HeLa cell expressing the C3 toxin, following γ -radiation. (c) Immunoblotting analysis of the effects of γ -radiation (15 Gy) on phosphorylated Chk1/Chk2 and histone H2AX levels in HeLa cells expressing the C3 toxin (using α -Tubulin as a loading control). (d) and (e) Assays for GFP-based detection of homologous recombination (HR, using HeLa-DR-GFP) or nonhomologous end joining (NHEJ, using HeLa-EJ5-GFP) after DNA damage induced by I- Sce I restriction enzyme expression. (d) Phase contrast (left) and green fluorescence (right) images of cells transfected with a plasmid for I- Sce I expression (I- Sce I), or with an empty vector (EV), showing the appearance of GFP-positive cells indicative of HR (HeLa-EJ5-GFP) or NHEJ (HeLa-DR-GFP), 72 h after transfection. (e) Quantification of HR and NHEJ assays, with (EV + C3 and I- Sce I + C3 groups) or without (EV and I- Sce I groups) concomitant C3 toxin expression. Graphs (with mean ± SD values) and immunoblots are representative of three independent experiments. ∗ P
    Rhoa Gtp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A : mRNA levels for inducible nitric oxide (NO) synthase (iNOS) and GTP cyclohydrolase (GTPCH) in cytokine-stimulated cardiac myocytes for 12 h. Similar findings were observed at 18 h (not shown). Ctrl, control. B : Western blots for protein expression

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes

    doi: 10.1152/ajpheart.00748.2008

    Figure Lengend Snippet: A : mRNA levels for inducible nitric oxide (NO) synthase (iNOS) and GTP cyclohydrolase (GTPCH) in cytokine-stimulated cardiac myocytes for 12 h. Similar findings were observed at 18 h (not shown). Ctrl, control. B : Western blots for protein expression

    Article Snippet: Maita N, Hatakeyama K, Okada K, Hakoshima T. Structural basis of biopterin-induced inhibition of GTP cyclohydrolase I by GFRP, its feeback regulatory protein.

    Techniques: Western Blot, Expressing

    GTP cyclohydrolase expression is comparable in male and female SHR GTP cyclohydrolase 1 protein was measured by Western blot analysis in renal IM of vehicle control male and female SHR ( n =8). Data are shown normalized to actin.

    Journal: Bioscience Reports

    Article Title: Oxidative stress induces BH4 deficiency in male, but not female, SHR

    doi: 10.1042/BSR20180111

    Figure Lengend Snippet: GTP cyclohydrolase expression is comparable in male and female SHR GTP cyclohydrolase 1 protein was measured by Western blot analysis in renal IM of vehicle control male and female SHR ( n =8). Data are shown normalized to actin.

    Article Snippet: Briefly, protein expression was determined using two-color immunoblots using primary antibodies to GTP cyclohydrolase-1 (GTPCH1; 1:250, Santa Cruz Biotechnology, Santa Cruz, CA, 100 μg protein/well) and β-actin (A1978, 1:10000; Sigma, St. Louis, MO).

    Techniques: Expressing, Western Blot

    Inhibition of RhoA activity by C3 toxin expression strongly affects DNA damage response, including global and specific DNA repair mechanisms in HeLa cells, following γ -radiation. (a) Dendritic morphology of HeLa cells (HeLa + C3 images) associated with decreased RhoA-GTP levels (on the right), 24 h after transfection with a plasmid for C3 toxin expression. Images on the right are insets from those on the left, 200x. (b) Estimates of DNA damage and repair efficiency (by olive tail moment, or OTM, measurements from comet assays) in HeLa cell expressing the C3 toxin, following γ -radiation. (c) Immunoblotting analysis of the effects of γ -radiation (15 Gy) on phosphorylated Chk1/Chk2 and histone H2AX levels in HeLa cells expressing the C3 toxin (using α -Tubulin as a loading control). (d) and (e) Assays for GFP-based detection of homologous recombination (HR, using HeLa-DR-GFP) or nonhomologous end joining (NHEJ, using HeLa-EJ5-GFP) after DNA damage induced by I- Sce I restriction enzyme expression. (d) Phase contrast (left) and green fluorescence (right) images of cells transfected with a plasmid for I- Sce I expression (I- Sce I), or with an empty vector (EV), showing the appearance of GFP-positive cells indicative of HR (HeLa-EJ5-GFP) or NHEJ (HeLa-DR-GFP), 72 h after transfection. (e) Quantification of HR and NHEJ assays, with (EV + C3 and I- Sce I + C3 groups) or without (EV and I- Sce I groups) concomitant C3 toxin expression. Graphs (with mean ± SD values) and immunoblots are representative of three independent experiments. ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Modulation of RhoA GTPase Activity Sensitizes Human Cervix Carcinoma Cells to γ-Radiation by Attenuating DNA Repair Pathways

    doi: 10.1155/2016/6012642

    Figure Lengend Snippet: Inhibition of RhoA activity by C3 toxin expression strongly affects DNA damage response, including global and specific DNA repair mechanisms in HeLa cells, following γ -radiation. (a) Dendritic morphology of HeLa cells (HeLa + C3 images) associated with decreased RhoA-GTP levels (on the right), 24 h after transfection with a plasmid for C3 toxin expression. Images on the right are insets from those on the left, 200x. (b) Estimates of DNA damage and repair efficiency (by olive tail moment, or OTM, measurements from comet assays) in HeLa cell expressing the C3 toxin, following γ -radiation. (c) Immunoblotting analysis of the effects of γ -radiation (15 Gy) on phosphorylated Chk1/Chk2 and histone H2AX levels in HeLa cells expressing the C3 toxin (using α -Tubulin as a loading control). (d) and (e) Assays for GFP-based detection of homologous recombination (HR, using HeLa-DR-GFP) or nonhomologous end joining (NHEJ, using HeLa-EJ5-GFP) after DNA damage induced by I- Sce I restriction enzyme expression. (d) Phase contrast (left) and green fluorescence (right) images of cells transfected with a plasmid for I- Sce I expression (I- Sce I), or with an empty vector (EV), showing the appearance of GFP-positive cells indicative of HR (HeLa-EJ5-GFP) or NHEJ (HeLa-DR-GFP), 72 h after transfection. (e) Quantification of HR and NHEJ assays, with (EV + C3 and I- Sce I + C3 groups) or without (EV and I- Sce I groups) concomitant C3 toxin expression. Graphs (with mean ± SD values) and immunoblots are representative of three independent experiments. ∗ P

    Article Snippet: RhoA-GTP bound to RBD-GST-Sepharose beads was resolved on 13% SDS-PAGE gels, transferred to nitrocellulose membranes and analyzed using a monoclonal anti-RhoA antibody (26C4, from Santa Cruz Biotechnology, Santa Cruz, CA, USA), as described below (see ).

    Techniques: Inhibition, Activity Assay, Expressing, Transfection, Plasmid Preparation, Homologous Recombination, Non-Homologous End Joining, Fluorescence, Western Blot

    Morphological Rho activity and proliferation analyses of parental and clonal HeLa cell lines expressing RhoA-N19 or RhoA-V14 mutants after γ -radiation. (a) Morphology of parental and derived HeLa cell lines. (b) Immunoblotting of pull-down assays for active RhoA (RhoA-GTP) in different cell lines. (c) and (d) Growth curves (c) and clonogenic assays (d) in cell lines under positive or negative modulation of RhoA activity. (e) Cell cycle profiles by flow cytometry analysis (using PI staining) of HeLa cell lines after exposure to different doses of γ -radiation. Graphs display mean ± SD of at least three independent experiments. ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Modulation of RhoA GTPase Activity Sensitizes Human Cervix Carcinoma Cells to γ-Radiation by Attenuating DNA Repair Pathways

    doi: 10.1155/2016/6012642

    Figure Lengend Snippet: Morphological Rho activity and proliferation analyses of parental and clonal HeLa cell lines expressing RhoA-N19 or RhoA-V14 mutants after γ -radiation. (a) Morphology of parental and derived HeLa cell lines. (b) Immunoblotting of pull-down assays for active RhoA (RhoA-GTP) in different cell lines. (c) and (d) Growth curves (c) and clonogenic assays (d) in cell lines under positive or negative modulation of RhoA activity. (e) Cell cycle profiles by flow cytometry analysis (using PI staining) of HeLa cell lines after exposure to different doses of γ -radiation. Graphs display mean ± SD of at least three independent experiments. ∗ P

    Article Snippet: RhoA-GTP bound to RBD-GST-Sepharose beads was resolved on 13% SDS-PAGE gels, transferred to nitrocellulose membranes and analyzed using a monoclonal anti-RhoA antibody (26C4, from Santa Cruz Biotechnology, Santa Cruz, CA, USA), as described below (see ).

    Techniques: Activity Assay, Expressing, Derivative Assay, Flow Cytometry, Cytometry, Staining