Journal: Cell metabolism

Article Title: Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Nonalcoholic Steatohepatitis

doi: 10.1016/j.cmet.2020.03.010

Figure Lengend Snippet: (A) RhoA activity (left graph, G-LISA® assay; right graph, GTP-RhoA precipitation using GSTRhotekin-RBD) of AML12 cells incubated for 2 h with vehicle or liposomes, or for 24 h with liposomes and then 2 h with Lipo-Chol. For the G-LISA® assay, n = 4 biological replicates; values shown are means ± SEM; *p < 0.05.

Article Snippet: Immunoprecipitation, LATS2 Kinase Assay, and GTP-RhoA Assay AML12 cells from 100-mm culture dishes were collected into 0.4 ml 1x ice-cold cell lysis buffer (#9803S, Cell Signaling) and incubated on ice for 5 min. Then cell lysates were centrifuged at 10,000 x g for 10 min at 4°C.

Techniques: Activity Assay, Incubation

Journal: Cell metabolism

Article Title: Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Nonalcoholic Steatohepatitis

doi: 10.1016/j.cmet.2020.03.010

Figure Lengend Snippet: (A) Images and mean fluorescence intensity (MFI) per cell of AML12 cells transduced with cyto-GCaMP6f or ER-GCaMP6f and then incubated for 30 min with vehicle or liposomes (n = 4 biological replicates; means ± SEM; *p < 0.05). Scale bar, 100 μm.

Article Snippet: Immunoprecipitation, LATS2 Kinase Assay, and GTP-RhoA Assay AML12 cells from 100-mm culture dishes were collected into 0.4 ml 1x ice-cold cell lysis buffer (#9803S, Cell Signaling) and incubated on ice for 5 min. Then cell lysates were centrifuged at 10,000 x g for 10 min at 4°C.

Techniques: Fluorescence, Transduction, Incubation

Journal: Cell metabolism

Article Title: Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Nonalcoholic Steatohepatitis

doi: 10.1016/j.cmet.2020.03.010

Figure Lengend Snippet: (A) TAZ immunoblot of siScr- or siGnas-transfected AML12 cells that were incubated for 16 h with liposomes and then 8 h with Lipo-Chol).

Article Snippet: Immunoprecipitation, LATS2 Kinase Assay, and GTP-RhoA Assay AML12 cells from 100-mm culture dishes were collected into 0.4 ml 1x ice-cold cell lysis buffer (#9803S, Cell Signaling) and incubated on ice for 5 min. Then cell lysates were centrifuged at 10,000 x g for 10 min at 4°C.

Techniques: Western Blot, Transfection, Incubation

Journal: Cell metabolism

Article Title: Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Nonalcoholic Steatohepatitis

doi: 10.1016/j.cmet.2020.03.010

Figure Lengend Snippet: (A) RhoA activity of AML12 cells that were incubated for 24 h with vehicle or liposomes or for 2 h with liposomes and then 3 h with Lipo-Chol ± 10 μM ALOD4 (n = 6 biological replicates; means ± SEM; ***p < 0.001 vs. both Veh groups).

Article Snippet: Immunoprecipitation, LATS2 Kinase Assay, and GTP-RhoA Assay AML12 cells from 100-mm culture dishes were collected into 0.4 ml 1x ice-cold cell lysis buffer (#9803S, Cell Signaling) and incubated on ice for 5 min. Then cell lysates were centrifuged at 10,000 x g for 10 min at 4°C.

Techniques: Activity Assay, Incubation