gtp levels  (Santa Cruz Biotechnology)

 
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    Name:
    Guanosine 5 Triphosphate Disodium Salt
    Description:
    Guanosine 5 Triphosphate Disodium Salt is a phosphoryl donor in protein synthesis and in signal transduction
    Catalog Number:
    SC-295030
    Price:
    None
    Category:
    Chemicals Other Chemicals Nucleic Acids Nucleotides and Nucleosides Guanosine 5 Triphosphate Disodium Salt
    Applications:
    Guanosine 5′-Triphosphate, Disodium Salt is a phosphoryl donor in protein synthesis and signal transduction
    Molecular Weight:
    567.14
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    Structured Review

    Santa Cruz Biotechnology gtp levels
    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. <t>Rap•GTP</t> was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.
    Guanosine 5 Triphosphate Disodium Salt is a phosphoryl donor in protein synthesis and in signal transduction
    https://www.bioz.com/result/gtp levels/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gtp levels - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells"

    Article Title: A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells

    Journal: Cells

    doi: 10.3390/cells8121589

    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. Rap•GTP was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.
    Figure Legend Snippet: Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. Rap•GTP was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.

    Techniques Used: Activation Assay, Stable Transfection, Expressing, Electrophoretic Mobility Shift Assay

    Related Articles

    Modification:

    Article Title: DLGAP1 directs megakaryocytic growth and differentiation in an MPL dependent manner in hematopoietic cells
    Article Snippet: .. When indicated, culture medium was supplemented with one of the following reagents: 10 pg/ml AP20187 - a dimerizer of proteins containing an F36 V domain, which is a modified FKBP-binding domain [ ] (ARIAD Pharmaceuticals, Cambridge, MA), 1 μM Imatinib (Novartis, Basel, Switzerland), 10 pg/ml GMCSF, 50 ng/ml PEG-rhMGDF, 400 mg/ml Geneticin (Invitrogen, Carlsbad, CA), 50 μM AG490 (Cayman Chemical Co., Ann Arbor, MI [Cayman]), 2.5 μM SU6656 (Cayman), 20 μM U0126 (Cell Signaling Technology, Boston, MA), 100 ng/ml Nocodazole (SCBT), 40 nM PMA (SCBT), 200 μM GTP (SCBT), 50 μM Olomoucine (SCBT), 50 μM iso-Olomoucine (SCBT) and 4.5 μM or 9 μM RO3306 (SCBT). ..

    Western Blot:

    Article Title: A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells
    Article Snippet: .. In brief, after 20 min of stimulation, cells were lysed and Rap•GTP levels determined by precipitating GTP-bound Rap and Western blot analysis with a α-Rap antibody (Santa Cruz Biotechnology, USA). ..

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  • 92
    Santa Cruz Biotechnology gtp levels
    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. <t>Rap•GTP</t> was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.
    Gtp Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gtp levels/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gtp levels - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

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    Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. Rap•GTP was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.

    Journal: Cells

    Article Title: A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells

    doi: 10.3390/cells8121589

    Figure Lengend Snippet: Rap and PKA activation in living cells. U2OS cells stably expressing Epac1 or Epac2 were stimulated as indicated. The activation of PKA was monitored by a phosphorylation-induced band shift of VASP. Rap•GTP was precipitated from cell lysates and compared to the total Rap levels. control, mock-stimulated; Fsk/IBMX, 15 μM forskolin and 200 μM IBMX to elevate intracellular cAMP levels.

    Article Snippet: In brief, after 20 min of stimulation, cells were lysed and Rap•GTP levels determined by precipitating GTP-bound Rap and Western blot analysis with a α-Rap antibody (Santa Cruz Biotechnology, USA).

    Techniques: Activation Assay, Stable Transfection, Expressing, Electrophoretic Mobility Shift Assay