marker isolectin b4 (Vector Laboratories)

Vector Laboratories is a verified supplier

Vector Laboratories manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Vector Laboratories marker isolectin b4
Marker Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gsl/Biotinylated+Griffonia+Simplicifolia+Lectin+I+(GSL+I)+isolectin+B4/pmc13133793-44-48-54
Average 95 stars, based on 667 article reviews

marker isolectin b4 - by Bioz Stars, 2026-09

95/100 stars

Images

Incubation:

Article Title: Migratory songbirds exhibit seasonal modulation of the oxygen cascade
Article Snippet: After rinsing, slides were then incubated in blocking solution (Carbo-Free Blocking Solution, SP-5040, Vector Laboratories) for 30 min at room temperature. .. Slides were then incubated overnight at room temperature in a humidified chamber in a solution containing fluorescein-labelled GSL (20 μg ml −1 ; FL-11015, Vector Laboratories) and primary antibodies against laminin (to identify muscle bundle boundaries, 1:200 dilution; L-9393; Millipore, Billerica, MA, USA) in HEPES buffer with N -acetylgalactosamine (GalNac; 10 mmol l −1 HEPES, 150 mmol l −1 NaCl, 0.1 mmol l −1 CaCl 2 , 1 mmol l −1 GalNac, pH 7.5). .. The following morning, slides were rinsed well in PBS and then incubated in TPBS containing secondary antibodies against the laminin primary antibodies (Alexa Fluor 594, goat anti-rabbit IgG; A11037, Life Technologies, Mississauga, ON, Canada) for 1 h. Sections were then rinsed thoroughly in PBS and mounted with Vectashield (Vector Laboratories).

Article Title: Cell-Penetrating Chaperone Nuc1 for Small- and Large-Molecule Delivery Into Retinal Cells and Tissues
Article Snippet: .. The RPE/choroid tissue was incubated with α-SMA antibody at 4°C overnight, followed with Cy3-labeled secondary antibody along with fluorescein–Griffonia Simplicifolia Lectin I (GSL I; 10 mg/mL in PBS; Vector Laboratories) for 2 hours at room temperature. .. Fluorescein–GSL I–stained choroidal neovascularization (CNV) lesions or Cy3-stained fibrotic spots were imaged on the RPE/choroid using a fluorescence microscope and the area of neovascularization and fibrosis were quantified using ImageJ software ( http://rsbweb.nih.gov/ij/ ).

Article Title: Migratory songbirds exhibit seasonal modulation of the oxygen cascade.
Article Snippet: After rinsing, slides were then incubated in blocking solution (Carbo-Free Blocking Solution, SP-5040, Vector Laboratories) for 30 min at room temperature. .. Slides were then incubated overnight at room temperature in a humidified chamber in a solution containing fluorescein-labelled GSL (20 μg ml−1; FL-11015, Vector Laboratories) and primary antibodies against laminin (to identify muscle bundle boundaries, 1:200 dilution; L-9393; Millipore, Billerica, MA, USA) in HEPES buffer with N-acetylgalactosamine (GalNac; 10 mmol l−1 HEPES, 150 mmol l−1 NaCl, 0.1 mmol l−1 CaCl2, 1 mmol l −1 GalNac, pH 7.5). .. The following morning, slides were rinsed well in PBS and then incubated in TPBS containing secondary antibodies against the laminin primary antibodies (Alexa Fluor 594, goat anti-rabbit IgG; A11037, Life Technologies, Mississauga, ON, Canada) for 1 h. Sections were then rinsed thoroughly in PBS and mounted with Vectashield (Vector Laboratories).

Article Title: Hybrid endometrial-derived hydrogel and human endometrial organoids synergize for uterine regeneration in an immunocompetent murine model
Article Snippet: .. To detect new blood vessels, the sections were incubated overnight at 4 °C with fluorescein-labeled Griffonia Simplicifolia Lectin I (GSL I, 1:200, FL-1101; Vector Laboratories, Burlingame, CA, USA). .. On the following day, sections were washed twice with PBS and incubated with alpha-smooth muscle actin (α-SMA, 1:200, C6198; Sigma-Aldrich) (30 min, RT).

Article Title: Hybrid endometrial-derived hydrogel and human endometrial organoids synergize for uterine regeneration in an immunocompetent murine model.
Article Snippet: .. To detect new blood vessels, the sections were incubated 363 overnight at 4°C with fluorescein-labeled Griffonia Simplicifolia Lectin 364 I (GSL I, 1:200, FL-1101; Vector Laboratories, Burlingame, CA, USA). .. 365 On the following day, sections were washed twice with PBS and 366 incubated with alpha-smooth muscle actin (α-SMA, 1:200, C6198; 367 Sigma-Aldrich) (30 min, RT).

Histopathology:

Article Title: Evidence of Silencing of Intestinal Inflammatory and Immune Transcripts Following Induction of Joint Inflammation
Article Snippet: Alcian Blue (Vector Labs, Alcian Blue (pH 2.5) Stain Kit, H-3501) was completed on consecutive sections for identification of goblet cells and mucus layers. .. Histopathology for three biotinylated mucin O-linked specific lectins: Griffonia (Bandeiraea) Simplidifolia Lectin I (GSL I, Vector Labs, B-1105-2); Maackia Amurensis Lectin II (MAL II, Vector Labs, B-1265-1) and Ulex Europaeus Agglutinin I (UEA I, Vector Labs, B-1065-2) were also completed on adjacent sections. ..

Staining:

Article Title: Evidence of an allostatic response by intestinal tissues following induction of joint inflammation
Article Snippet: Sagittal sections from knee joints were stained with Hematoxylin/Eosin, anti-CD68 (Abcam, Ab283654 ; 1:100) or anti-CD4 Abcam, Ab237722 1:2000). .. Sections of intestinal Swiss rolls were stained using the Alcian Blue Kit (Vector Labs, H-3501), anti-CD68 (1:100), anti-CD4 (1:2000), anti-CD8 (Abcam, Ab237709 , 1:500) or anti-Ki67 (Invitrogen, MA5–14520; 1:100), Invitrogen, MA5–14520) or the following biotinylated lectins (all from Vector Labs), Griffonia Bandeiraea Simplidifolia Lectin I, (GSL I, B-1105–2), Maackia Amurensis Lectin II (MAL II, B-1265–1) and Ulex Europaeus Agglutinin I (UEA, B-1065–2). .. Prior to histochemical staining, antigen retrieval was performed using Tris/EDTA (Abcam, ab93684), and sections were blocked with normal goat serum (Vector Laboratories, S-1000).

Review

marker isolectin b4 (Vector Laboratories)

Structured Review

Images

Related Articles

Incubation:

Histopathology:

Staining:

Similar Products

Image Search Results