gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    griffonia simplicifolia lectin gsl i isolectin b4 conjugated  (Vector Laboratories)


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    Vector Laboratories griffonia simplicifolia lectin gsl i isolectin b4 conjugated
    Griffonia Simplicifolia Lectin Gsl I Isolectin B4 Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
    A. Left: Heatmap shows RNA-seq transcript levels of AP-2, EGFR, and EGF family members in P4 skin and the epidermis of 3-week-old, 6-week-old, and 22-month-old WT mice. Middle: Pie charts show relative contributions of AP-2 family members to overall AP-2 transcript levels in epidermis of 3-week-old, 6-week-old, and 22-month-old WT mice. Immunolabeling of adult mouse back skin shows that AP–2α and AP–2β are located in the nuclei of basal and suprabasal keratinocytes. <t>Isolectin</t> <t>B4-DyLight649</t> (white). Scale bars, 50 mm. Right: Effects of inactivation of AP–2α/AP–2β in keratinocytes on transcript levels of AP-2, EGFR, and EGF family members in 3-week-old and 6-month-old epidermis. B. Left: Effects of inactivation of AP–2α/AP–2β in keratinocytes on transcript levels of AP-2, EGFR, KCTD1 and KCTD15 (regulators of AP-2 activity), and EGF family members in 3-week-old and 6-month-old epidermis. Right: Values shown for 3-week-old epidermis lacking AP–2α or AP–2β in keratinocytes. Log2FC and adjusted p-values (padj) are shown. C. Immunolabeling for AP–2α and AP–2β shows overlapping expression in adult keratinocytes. Back skin of adult WT mice shown. Yellow arrows: interfollicular epidermis. the dermal papilla is not targeted by K14Cre and AP–2α is maintained in the dermal papilla of K14Cre + Tfap2a fl/fl mice [white arrow] that lack AP–2α immunolabeling in keratinocytes [red arrow]). Inactivation of Kctd1 and Kctd15 function in keratinocytes does not affect nuclear localization of AP–2α and AP–2β. Scale bars, 50 mm. D. Transepidermal water loss (TEWL in g/m 2 h) measurements of back skin in newborn (P0) K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice and control littermates. Mean ± SEM; p-value shown for two-sided t-test. E. Body weight (BW) of male K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice and control littermates at P28-P30. Mean ± SEM; p-value shown for two-sided t-test. F. Gender- and age-matched 1-month-old K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl Tfap2b fl/WT mice, and control littermates shown. Hairs show irregular morphology with kinks, bends, and abnormal hair shaft thickness in P29 K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice (green arrow). Typical zigzag hairs, seen in WT controls (black arrow), are not observed in K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice. G. Top: Immunolabeling for keratin 5 (K5; green) and loricrin (red) in back skin of 1-month-old K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl mice, K14Cre + Tfap2b fl/fl mice, and control mice. Bottom: Immunolabeling for Ki67 (red) and labeling with isolectin B4-FITC (green) in the same specimens. Arrows indicate Ki67 + cells. Scale bars, 50 mm. H. Representative images from 2-month-old ear skin of K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl mice, and control mice. Arrows indicate epidermis. Scale bar, 250 mm. I. P7 and P13 K14Cre + Tfap2a fl/fl mice and control littermates are shown. AP–2α inactivation in keratinocytes results in a postnatal growth retardation, delayed hair growth and abnormal morphology of hairs and whiskers.
    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "AP-2α/AP-2β transcription factors are key regulators of epidermal homeostasis"

    Article Title: AP-2α/AP-2β transcription factors are key regulators of epidermal homeostasis

    Journal: bioRxiv

    doi: 10.1101/2023.12.03.569763

    A. Left: Heatmap shows RNA-seq transcript levels of AP-2, EGFR, and EGF family members in P4 skin and the epidermis of 3-week-old, 6-week-old, and 22-month-old WT mice. Middle: Pie charts show relative contributions of AP-2 family members to overall AP-2 transcript levels in epidermis of 3-week-old, 6-week-old, and 22-month-old WT mice. Immunolabeling of adult mouse back skin shows that AP–2α and AP–2β are located in the nuclei of basal and suprabasal keratinocytes. Isolectin B4-DyLight649 (white). Scale bars, 50 mm. Right: Effects of inactivation of AP–2α/AP–2β in keratinocytes on transcript levels of AP-2, EGFR, and EGF family members in 3-week-old and 6-month-old epidermis. B. Left: Effects of inactivation of AP–2α/AP–2β in keratinocytes on transcript levels of AP-2, EGFR, KCTD1 and KCTD15 (regulators of AP-2 activity), and EGF family members in 3-week-old and 6-month-old epidermis. Right: Values shown for 3-week-old epidermis lacking AP–2α or AP–2β in keratinocytes. Log2FC and adjusted p-values (padj) are shown. C. Immunolabeling for AP–2α and AP–2β shows overlapping expression in adult keratinocytes. Back skin of adult WT mice shown. Yellow arrows: interfollicular epidermis. the dermal papilla is not targeted by K14Cre and AP–2α is maintained in the dermal papilla of K14Cre + Tfap2a fl/fl mice [white arrow] that lack AP–2α immunolabeling in keratinocytes [red arrow]). Inactivation of Kctd1 and Kctd15 function in keratinocytes does not affect nuclear localization of AP–2α and AP–2β. Scale bars, 50 mm. D. Transepidermal water loss (TEWL in g/m 2 h) measurements of back skin in newborn (P0) K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice and control littermates. Mean ± SEM; p-value shown for two-sided t-test. E. Body weight (BW) of male K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice and control littermates at P28-P30. Mean ± SEM; p-value shown for two-sided t-test. F. Gender- and age-matched 1-month-old K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl Tfap2b fl/WT mice, and control littermates shown. Hairs show irregular morphology with kinks, bends, and abnormal hair shaft thickness in P29 K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice (green arrow). Typical zigzag hairs, seen in WT controls (black arrow), are not observed in K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice. G. Top: Immunolabeling for keratin 5 (K5; green) and loricrin (red) in back skin of 1-month-old K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl mice, K14Cre + Tfap2b fl/fl mice, and control mice. Bottom: Immunolabeling for Ki67 (red) and labeling with isolectin B4-FITC (green) in the same specimens. Arrows indicate Ki67 + cells. Scale bars, 50 mm. H. Representative images from 2-month-old ear skin of K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl mice, and control mice. Arrows indicate epidermis. Scale bar, 250 mm. I. P7 and P13 K14Cre + Tfap2a fl/fl mice and control littermates are shown. AP–2α inactivation in keratinocytes results in a postnatal growth retardation, delayed hair growth and abnormal morphology of hairs and whiskers.
    Figure Legend Snippet: A. Left: Heatmap shows RNA-seq transcript levels of AP-2, EGFR, and EGF family members in P4 skin and the epidermis of 3-week-old, 6-week-old, and 22-month-old WT mice. Middle: Pie charts show relative contributions of AP-2 family members to overall AP-2 transcript levels in epidermis of 3-week-old, 6-week-old, and 22-month-old WT mice. Immunolabeling of adult mouse back skin shows that AP–2α and AP–2β are located in the nuclei of basal and suprabasal keratinocytes. Isolectin B4-DyLight649 (white). Scale bars, 50 mm. Right: Effects of inactivation of AP–2α/AP–2β in keratinocytes on transcript levels of AP-2, EGFR, and EGF family members in 3-week-old and 6-month-old epidermis. B. Left: Effects of inactivation of AP–2α/AP–2β in keratinocytes on transcript levels of AP-2, EGFR, KCTD1 and KCTD15 (regulators of AP-2 activity), and EGF family members in 3-week-old and 6-month-old epidermis. Right: Values shown for 3-week-old epidermis lacking AP–2α or AP–2β in keratinocytes. Log2FC and adjusted p-values (padj) are shown. C. Immunolabeling for AP–2α and AP–2β shows overlapping expression in adult keratinocytes. Back skin of adult WT mice shown. Yellow arrows: interfollicular epidermis. the dermal papilla is not targeted by K14Cre and AP–2α is maintained in the dermal papilla of K14Cre + Tfap2a fl/fl mice [white arrow] that lack AP–2α immunolabeling in keratinocytes [red arrow]). Inactivation of Kctd1 and Kctd15 function in keratinocytes does not affect nuclear localization of AP–2α and AP–2β. Scale bars, 50 mm. D. Transepidermal water loss (TEWL in g/m 2 h) measurements of back skin in newborn (P0) K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice and control littermates. Mean ± SEM; p-value shown for two-sided t-test. E. Body weight (BW) of male K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice and control littermates at P28-P30. Mean ± SEM; p-value shown for two-sided t-test. F. Gender- and age-matched 1-month-old K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl Tfap2b fl/WT mice, and control littermates shown. Hairs show irregular morphology with kinks, bends, and abnormal hair shaft thickness in P29 K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice (green arrow). Typical zigzag hairs, seen in WT controls (black arrow), are not observed in K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice. G. Top: Immunolabeling for keratin 5 (K5; green) and loricrin (red) in back skin of 1-month-old K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl mice, K14Cre + Tfap2b fl/fl mice, and control mice. Bottom: Immunolabeling for Ki67 (red) and labeling with isolectin B4-FITC (green) in the same specimens. Arrows indicate Ki67 + cells. Scale bars, 50 mm. H. Representative images from 2-month-old ear skin of K14Cre + Tfap2a fl/fl Tfap2b fl/fl mice, K14Cre + Tfap2a fl/fl mice, and control mice. Arrows indicate epidermis. Scale bar, 250 mm. I. P7 and P13 K14Cre + Tfap2a fl/fl mice and control littermates are shown. AP–2α inactivation in keratinocytes results in a postnatal growth retardation, delayed hair growth and abnormal morphology of hairs and whiskers.

    Techniques Used: RNA Sequencing Assay, Immunolabeling, Activity Assay, Expressing, Labeling

    gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
    A) Violin plots showing Tm4sf1 expression mainly in arterial and venous ECs, pericytes and mesothelial cells, with the aAECs exhibiting the highest expression. Immunofluorescent staining for TM4SF1 with co-staining for ECs <t>(isolectin</t> <t>B4),</t> pericytes (3G5), or smooth muscle cells (SMA) in (B) healthy lungs (control) or (C) at 5-weeks in the SU/CH model. D) Immunofluorescent staining of lung sections from control subjects and PAH patients for TM4SF1, co-stained for ECs (CD31), demonstrating abundant TM4SF1 expression in PAH associated remodeled arteries relative to controls. Scale bar represents 20μm (B, C) and 50μm (D).
    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Emergence of disease specific endothelial and stromal cell populations involved in arterial remodeling during development of pulmonary arterial hypertension"

    Article Title: Emergence of disease specific endothelial and stromal cell populations involved in arterial remodeling during development of pulmonary arterial hypertension

    Journal: bioRxiv

    doi: 10.1101/2023.09.06.555321

    A) Violin plots showing Tm4sf1 expression mainly in arterial and venous ECs, pericytes and mesothelial cells, with the aAECs exhibiting the highest expression. Immunofluorescent staining for TM4SF1 with co-staining for ECs (isolectin B4), pericytes (3G5), or smooth muscle cells (SMA) in (B) healthy lungs (control) or (C) at 5-weeks in the SU/CH model. D) Immunofluorescent staining of lung sections from control subjects and PAH patients for TM4SF1, co-stained for ECs (CD31), demonstrating abundant TM4SF1 expression in PAH associated remodeled arteries relative to controls. Scale bar represents 20μm (B, C) and 50μm (D).
    Figure Legend Snippet: A) Violin plots showing Tm4sf1 expression mainly in arterial and venous ECs, pericytes and mesothelial cells, with the aAECs exhibiting the highest expression. Immunofluorescent staining for TM4SF1 with co-staining for ECs (isolectin B4), pericytes (3G5), or smooth muscle cells (SMA) in (B) healthy lungs (control) or (C) at 5-weeks in the SU/CH model. D) Immunofluorescent staining of lung sections from control subjects and PAH patients for TM4SF1, co-stained for ECs (CD31), demonstrating abundant TM4SF1 expression in PAH associated remodeled arteries relative to controls. Scale bar represents 20μm (B, C) and 50μm (D).

    Techniques Used: Expressing, Staining

    gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dylight594 conjugated gsl ib4  (Vector Laboratories)


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    Vector Laboratories dylight594 conjugated gsl ib4
    Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the <t>GSL-IB4-positive</t> area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.
    Dylight594 Conjugated Gsl Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Spatiotemporal changes of tissue glycans depending on localization in cardiac aging"

    Article Title: Spatiotemporal changes of tissue glycans depending on localization in cardiac aging

    Journal: Regenerative Therapy

    doi: 10.1016/j.reth.2022.12.009

    Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the GSL-IB4-positive area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.
    Figure Legend Snippet: Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the GSL-IB4-positive area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.

    Techniques Used: Staining

    gsl i b4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b4 isolectin conjugated
    Gsl I B4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gsl i b 4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated isolectin b4  (Vector Laboratories)


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    Vector Laboratories biotinylated isolectin b4
    Biotinylated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories griffonia simplicifolia lectin gsl i isolectin b4 conjugated
    Griffonia Simplicifolia Lectin Gsl I Isolectin B4 Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories dylight594 conjugated gsl ib4
    Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the <t>GSL-IB4-positive</t> area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.
    Dylight594 Conjugated Gsl Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories gsl i b4 isolectin conjugated
    Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the <t>GSL-IB4-positive</t> area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.
    Gsl I B4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories gsl i b 4 isolectin conjugated
    Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the <t>GSL-IB4-positive</t> area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsl i b 4 isolectin conjugated/product/Vector Laboratories
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    Vector Laboratories biotinylated isolectin b4
    Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the <t>GSL-IB4-positive</t> area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.
    Biotinylated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the GSL-IB4-positive area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.

    Journal: Regenerative Therapy

    Article Title: Spatiotemporal changes of tissue glycans depending on localization in cardiac aging

    doi: 10.1016/j.reth.2022.12.009

    Figure Lengend Snippet: Decrease in the number of cardiac microvessels with aging (a) Papillary muscles were stained for Griffonia simplicifolia lectin I (GSL-I) B4 (red) and combined with anti-alpha smooth muscle actin (SMA) antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively) (60× magnification). Analysis was performed in the area without α-SMA staining. The values of the GSL-IB4-positive area of three female mice with seven to ten spots in every age group are shown in the box plot. (b) The left ventricular walls were stained for GSL-IB4 (red) and combined with anti-α-SMA antibody (green) and DAPI (blue) signals in samples from mice aged 2, 6, 12–14, and 23–25 months (upper and lower, respectively). The ratios of the GSL-IB4-positive area to the total area of three female mice with six to nine spots in every age group are shown in the box plot. Analysis was performed in the area without α-SMA staining. P < 0.05. N.S. = no significant difference. Scale bars are 50 μm.

    Article Snippet: Following a wash step with PBS, the sections were incubated with 10 μg/mL of Dylight594-conjugated GSL-IB4 (#DL-1207; Vector Laboratories Inc.) in combination with anti-α-SMA antibody at 4 °C overnight in PBS.

    Techniques: Staining