gsl i b 4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gsl i b 4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotin conjugated griffonia simplicifolia lectin gsl i isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotin conjugated griffonia simplicifolia lectin gsl i isolectin b 4
    Biotin Conjugated Griffonia Simplicifolia Lectin Gsl I Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bsi b 4 conjugated to dylight 594  (Vector Laboratories)


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    Vector Laboratories bsi b 4 conjugated to dylight 594
    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." width="250" height="auto" />
    Bsi B 4 Conjugated To Dylight 594, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria"

    Article Title: A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.958952

    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." title="No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only.

    Techniques Used: Binding Assay, Cell Culture, Staining, Dot Blot, Positive Control, Derivative Assay, SYBR Green Assay

    Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See <xref ref-type= Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version." title="Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version.

    Techniques Used: Dot Blot, Staining, Positive Control, Purification, Cell Culture, Knock-Out, Immunofluorescence, Microscopy, Fluorescence, Flow Cytometry, Expressing

    fluorescein isothiocyanate fict conjugated isolectin b 4  (Vector Laboratories)


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    Vector Laboratories fluorescein isothiocyanate fict conjugated isolectin b 4
    Effects of MMF application in a specific time window in NMDA damaged OHSC fixed at 9 div. (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and <t>IB</t> <t>4</t> (microglial and vessels, green) in overview and in higher magnification of the labeled area. Control (CTL, n = 5) OHSC showed a well preservation of the hippocampal formation with almost no PI positive pyknotic nuclei and only a few ramified IB 4 positive microglia. OHSC treated with NMDA ( n = 13) (50 μM) for 4 h had massive accumulation of PI positive degenerating neurons and amoeboid IB 4 positive microglia. Treatment with MMF (100 μg/ml) in defined time windows resulted in a reduction of PI positive neurons between 12 and 36 h ( n = 6) and 8 and 36 h ( n = 43) but not between 8 and 24 h ( n = 12) after NMDA damage. In all time windows, there was a reduction in the number of microglia. Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.
    Fluorescein Isothiocyanate Fict Conjugated Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Microglia-Dependent and Independent Brain Cytoprotective Effects of Mycophenolate Mofetil During Neuronal Damage"

    Article Title: Microglia-Dependent and Independent Brain Cytoprotective Effects of Mycophenolate Mofetil During Neuronal Damage

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2022.863598

    Effects of MMF application in a specific time window in NMDA damaged OHSC fixed at 9 div. (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial and vessels, green) in overview and in higher magnification of the labeled area. Control (CTL, n = 5) OHSC showed a well preservation of the hippocampal formation with almost no PI positive pyknotic nuclei and only a few ramified IB 4 positive microglia. OHSC treated with NMDA ( n = 13) (50 μM) for 4 h had massive accumulation of PI positive degenerating neurons and amoeboid IB 4 positive microglia. Treatment with MMF (100 μg/ml) in defined time windows resulted in a reduction of PI positive neurons between 12 and 36 h ( n = 6) and 8 and 36 h ( n = 43) but not between 8 and 24 h ( n = 12) after NMDA damage. In all time windows, there was a reduction in the number of microglia. Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.
    Figure Legend Snippet: Effects of MMF application in a specific time window in NMDA damaged OHSC fixed at 9 div. (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial and vessels, green) in overview and in higher magnification of the labeled area. Control (CTL, n = 5) OHSC showed a well preservation of the hippocampal formation with almost no PI positive pyknotic nuclei and only a few ramified IB 4 positive microglia. OHSC treated with NMDA ( n = 13) (50 μM) for 4 h had massive accumulation of PI positive degenerating neurons and amoeboid IB 4 positive microglia. Treatment with MMF (100 μg/ml) in defined time windows resulted in a reduction of PI positive neurons between 12 and 36 h ( n = 6) and 8 and 36 h ( n = 43) but not between 8 and 24 h ( n = 12) after NMDA damage. In all time windows, there was a reduction in the number of microglia. Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.

    Techniques Used: Staining, Labeling, Preserving

    Effects of continuous MMF treatment for OHSC fixed at 72 h after injury with and without microglia (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial cells vascular vessels, green) in overview and in higher magnification of the labeled area. In comparison to control (CTL, n = 9), treatment with NMDA for 4 h (NMDA, n = 6) led increase in number of PI positive degenerating neurons. Treatment with MMF (NMDA + MMF, n = 7) in a period between 4 and 72 h after the injury resulted in a reduction of PI positive degenerated neurons. Incubation with 100 μg/ml Clo for 6 days resulted in successful depletion of microglia from OHSC in the respective groups (Clo, n = 4; NMDA + Clo, n = 16; NMDA + Clo + MMF, n = 19). Additional application of MMF in this period led to reduction of PI positive degenerated neurons in microglia depleted OHSC (NMDA + Clo + MMF). Depletion of microglia led to an increase in number of damaged cells (NMDA + Clo, NMDA + Clo + MMF). Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA or NMDA + Clo) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.
    Figure Legend Snippet: Effects of continuous MMF treatment for OHSC fixed at 72 h after injury with and without microglia (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial cells vascular vessels, green) in overview and in higher magnification of the labeled area. In comparison to control (CTL, n = 9), treatment with NMDA for 4 h (NMDA, n = 6) led increase in number of PI positive degenerating neurons. Treatment with MMF (NMDA + MMF, n = 7) in a period between 4 and 72 h after the injury resulted in a reduction of PI positive degenerated neurons. Incubation with 100 μg/ml Clo for 6 days resulted in successful depletion of microglia from OHSC in the respective groups (Clo, n = 4; NMDA + Clo, n = 16; NMDA + Clo + MMF, n = 19). Additional application of MMF in this period led to reduction of PI positive degenerated neurons in microglia depleted OHSC (NMDA + Clo + MMF). Depletion of microglia led to an increase in number of damaged cells (NMDA + Clo, NMDA + Clo + MMF). Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA or NMDA + Clo) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.

    Techniques Used: Staining, Labeling, Incubation

    dylight 594 conjugated griffonia simplificolia isolectin b 4  (Vector Laboratories)


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    Vector Laboratories dylight 594 conjugated griffonia simplificolia isolectin b 4
    Qualitative images highlighting a few of the key anatomical differences between the fast twitch extensor digitorum longus (EDL) and slow twitch soleus muscles (A). From left to right: (Left) Vessel density images are z-projections of <t>Dylight</t> <t>594</t> conjugated lectin; (Middle) BODIPY images are z-projections of BODIPY positive lipid droplets. Red signal in BODIPY images are lectin stained blood vessels. Blue signal in BODIPY images are myonuclei. Arrows indicate BODIPY positive lipid droplets in zoomed image inlays; (Right) Mitochondrial NAD(P)H images are optical sections of reduced pyridine nucleotide autofluorescence in live isolated skeletal muscle. NAD(P)H fluorescence intensity is mapped onto the image (highest intensity in white). Scale bars are 25um.
    Dylight 594 Conjugated Griffonia Simplificolia Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Effects of fasting induced carbohydrate depletion on murine ischemic skeletal muscle function"

    Article Title: Effects of fasting induced carbohydrate depletion on murine ischemic skeletal muscle function

    Journal: bioRxiv

    doi: 10.1101/846774

    Qualitative images highlighting a few of the key anatomical differences between the fast twitch extensor digitorum longus (EDL) and slow twitch soleus muscles (A). From left to right: (Left) Vessel density images are z-projections of Dylight 594 conjugated lectin; (Middle) BODIPY images are z-projections of BODIPY positive lipid droplets. Red signal in BODIPY images are lectin stained blood vessels. Blue signal in BODIPY images are myonuclei. Arrows indicate BODIPY positive lipid droplets in zoomed image inlays; (Right) Mitochondrial NAD(P)H images are optical sections of reduced pyridine nucleotide autofluorescence in live isolated skeletal muscle. NAD(P)H fluorescence intensity is mapped onto the image (highest intensity in white). Scale bars are 25um.
    Figure Legend Snippet: Qualitative images highlighting a few of the key anatomical differences between the fast twitch extensor digitorum longus (EDL) and slow twitch soleus muscles (A). From left to right: (Left) Vessel density images are z-projections of Dylight 594 conjugated lectin; (Middle) BODIPY images are z-projections of BODIPY positive lipid droplets. Red signal in BODIPY images are lectin stained blood vessels. Blue signal in BODIPY images are myonuclei. Arrows indicate BODIPY positive lipid droplets in zoomed image inlays; (Right) Mitochondrial NAD(P)H images are optical sections of reduced pyridine nucleotide autofluorescence in live isolated skeletal muscle. NAD(P)H fluorescence intensity is mapped onto the image (highest intensity in white). Scale bars are 25um.

    Techniques Used: Staining, Isolation, Fluorescence

    griffonia simplicolia isolectin b 4 dylight594 conjugate  (Vector Laboratories)


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    Vector Laboratories griffonia simplicolia isolectin b 4 dylight594 conjugate
    Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected <t>Dylight594–conjugated</t> Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).
    Griffonia Simplicolia Isolectin B 4 Dylight594 Conjugate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/griffonia simplicolia isolectin b 4 dylight594 conjugate/product/Vector Laboratories
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    Images

    1) Product Images from "Strain-Dependent Variation in Acute Ischemic Muscle Injury"

    Article Title: Strain-Dependent Variation in Acute Ischemic Muscle Injury

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2018.01.008

    Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected Dylight594–conjugated Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).
    Figure Legend Snippet: Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected Dylight594–conjugated Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).

    Techniques Used: Immunofluorescence, Injection

    griffonia simplicolia isolectin b 4 dylight594 conjugate  (Vector Laboratories)


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    Vector Laboratories griffonia simplicolia isolectin b 4 dylight594 conjugate
    Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected <t>Dylight594–conjugated</t> Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).
    Griffonia Simplicolia Isolectin B 4 Dylight594 Conjugate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/griffonia simplicolia isolectin b 4 dylight594 conjugate/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    griffonia simplicolia isolectin b 4 dylight594 conjugate - by Bioz Stars, 2024-06
    94/100 stars

    Images

    1) Product Images from "Strain-Dependent Variation in Acute Ischemic Muscle Injury"

    Article Title: Strain-Dependent Variation in Acute Ischemic Muscle Injury

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2018.01.008

    Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected Dylight594–conjugated Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).
    Figure Legend Snippet: Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected Dylight594–conjugated Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).

    Techniques Used: Immunofluorescence, Injection

    biotin conjugated griffonia simplicifolia lectin i isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotin conjugated griffonia simplicifolia lectin i isolectin b 4
    Biotin Conjugated Griffonia Simplicifolia Lectin I Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories bsi b 4 conjugated to dylight 594
    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." width="250" height="auto" />
    Bsi B 4 Conjugated To Dylight 594, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories fluorescein isothiocyanate fict conjugated isolectin b 4
    Effects of MMF application in a specific time window in NMDA damaged OHSC fixed at 9 div. (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and <t>IB</t> <t>4</t> (microglial and vessels, green) in overview and in higher magnification of the labeled area. Control (CTL, n = 5) OHSC showed a well preservation of the hippocampal formation with almost no PI positive pyknotic nuclei and only a few ramified IB 4 positive microglia. OHSC treated with NMDA ( n = 13) (50 μM) for 4 h had massive accumulation of PI positive degenerating neurons and amoeboid IB 4 positive microglia. Treatment with MMF (100 μg/ml) in defined time windows resulted in a reduction of PI positive neurons between 12 and 36 h ( n = 6) and 8 and 36 h ( n = 43) but not between 8 and 24 h ( n = 12) after NMDA damage. In all time windows, there was a reduction in the number of microglia. Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.
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    Vector Laboratories dylight 594 conjugated griffonia simplificolia isolectin b 4
    Qualitative images highlighting a few of the key anatomical differences between the fast twitch extensor digitorum longus (EDL) and slow twitch soleus muscles (A). From left to right: (Left) Vessel density images are z-projections of <t>Dylight</t> <t>594</t> conjugated lectin; (Middle) BODIPY images are z-projections of BODIPY positive lipid droplets. Red signal in BODIPY images are lectin stained blood vessels. Blue signal in BODIPY images are myonuclei. Arrows indicate BODIPY positive lipid droplets in zoomed image inlays; (Right) Mitochondrial NAD(P)H images are optical sections of reduced pyridine nucleotide autofluorescence in live isolated skeletal muscle. NAD(P)H fluorescence intensity is mapped onto the image (highest intensity in white). Scale bars are 25um.
    Dylight 594 Conjugated Griffonia Simplificolia Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories griffonia simplicolia isolectin b 4 dylight594 conjugate
    Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected <t>Dylight594–conjugated</t> Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).
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    Vector Laboratories biotin conjugated griffonia simplicifolia lectin i isolectin b 4
    Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected <t>Dylight594–conjugated</t> Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).
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    Image Search Results


    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria

    doi: 10.3389/fimmu.2022.958952

    Figure Lengend Snippet: No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only.

    Article Snippet: 5x10 5 cells were seeded and stained with either 27H8 purified antibody or IgG1κ isotype control (clone: B3102E8, Southern Biotech) at 1 µg/ml, a 1:10 dilution of M86 supernatant, a 1:10 dilution (40 µg/ml) of mouse IgM isotype control (clone: MOPC 104E, Sigma) or a 1:100 dilution of BSI-B 4 conjugated to DyLight ® 594 ( Griffonia simplicifolia isolectin B4, Vector Laboratories, Burlingame, CA, USA) in BSA-buffer.

    Techniques: Binding Assay, Cell Culture, Staining, Dot Blot, Positive Control, Derivative Assay, SYBR Green Assay

    Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See <xref ref-type= Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version." width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria

    doi: 10.3389/fimmu.2022.958952

    Figure Lengend Snippet: Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version.

    Article Snippet: 5x10 5 cells were seeded and stained with either 27H8 purified antibody or IgG1κ isotype control (clone: B3102E8, Southern Biotech) at 1 µg/ml, a 1:10 dilution of M86 supernatant, a 1:10 dilution (40 µg/ml) of mouse IgM isotype control (clone: MOPC 104E, Sigma) or a 1:100 dilution of BSI-B 4 conjugated to DyLight ® 594 ( Griffonia simplicifolia isolectin B4, Vector Laboratories, Burlingame, CA, USA) in BSA-buffer.

    Techniques: Dot Blot, Staining, Positive Control, Purification, Cell Culture, Knock-Out, Immunofluorescence, Microscopy, Fluorescence, Flow Cytometry, Expressing

    Effects of MMF application in a specific time window in NMDA damaged OHSC fixed at 9 div. (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial and vessels, green) in overview and in higher magnification of the labeled area. Control (CTL, n = 5) OHSC showed a well preservation of the hippocampal formation with almost no PI positive pyknotic nuclei and only a few ramified IB 4 positive microglia. OHSC treated with NMDA ( n = 13) (50 μM) for 4 h had massive accumulation of PI positive degenerating neurons and amoeboid IB 4 positive microglia. Treatment with MMF (100 μg/ml) in defined time windows resulted in a reduction of PI positive neurons between 12 and 36 h ( n = 6) and 8 and 36 h ( n = 43) but not between 8 and 24 h ( n = 12) after NMDA damage. In all time windows, there was a reduction in the number of microglia. Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.

    Journal: Frontiers in Aging Neuroscience

    Article Title: Microglia-Dependent and Independent Brain Cytoprotective Effects of Mycophenolate Mofetil During Neuronal Damage

    doi: 10.3389/fnagi.2022.863598

    Figure Lengend Snippet: Effects of MMF application in a specific time window in NMDA damaged OHSC fixed at 9 div. (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial and vessels, green) in overview and in higher magnification of the labeled area. Control (CTL, n = 5) OHSC showed a well preservation of the hippocampal formation with almost no PI positive pyknotic nuclei and only a few ramified IB 4 positive microglia. OHSC treated with NMDA ( n = 13) (50 μM) for 4 h had massive accumulation of PI positive degenerating neurons and amoeboid IB 4 positive microglia. Treatment with MMF (100 μg/ml) in defined time windows resulted in a reduction of PI positive neurons between 12 and 36 h ( n = 6) and 8 and 36 h ( n = 43) but not between 8 and 24 h ( n = 12) after NMDA damage. In all time windows, there was a reduction in the number of microglia. Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.

    Article Snippet: Microglia were labeled with fluorescein isothiocyanate (FICT)-conjugated isolectin B 4 (IB 4 , Vector Laboratories, Burlingame, CA, United States) diluted 1:50 in PBS/Triton for 24 h. Before staining, the OHSC were incubated in normal goat serum (NGS, Sigma Aldrich, 1:20) in PB (0.2 M) for 30 min.

    Techniques: Staining, Labeling, Preserving

    Effects of continuous MMF treatment for OHSC fixed at 72 h after injury with and without microglia (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial cells vascular vessels, green) in overview and in higher magnification of the labeled area. In comparison to control (CTL, n = 9), treatment with NMDA for 4 h (NMDA, n = 6) led increase in number of PI positive degenerating neurons. Treatment with MMF (NMDA + MMF, n = 7) in a period between 4 and 72 h after the injury resulted in a reduction of PI positive degenerated neurons. Incubation with 100 μg/ml Clo for 6 days resulted in successful depletion of microglia from OHSC in the respective groups (Clo, n = 4; NMDA + Clo, n = 16; NMDA + Clo + MMF, n = 19). Additional application of MMF in this period led to reduction of PI positive degenerated neurons in microglia depleted OHSC (NMDA + Clo + MMF). Depletion of microglia led to an increase in number of damaged cells (NMDA + Clo, NMDA + Clo + MMF). Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA or NMDA + Clo) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.

    Journal: Frontiers in Aging Neuroscience

    Article Title: Microglia-Dependent and Independent Brain Cytoprotective Effects of Mycophenolate Mofetil During Neuronal Damage

    doi: 10.3389/fnagi.2022.863598

    Figure Lengend Snippet: Effects of continuous MMF treatment for OHSC fixed at 72 h after injury with and without microglia (A) Treatment protocol. (B) CLSM images stained with PI (degenerating neurons, red) and IB 4 (microglial cells vascular vessels, green) in overview and in higher magnification of the labeled area. In comparison to control (CTL, n = 9), treatment with NMDA for 4 h (NMDA, n = 6) led increase in number of PI positive degenerating neurons. Treatment with MMF (NMDA + MMF, n = 7) in a period between 4 and 72 h after the injury resulted in a reduction of PI positive degenerated neurons. Incubation with 100 μg/ml Clo for 6 days resulted in successful depletion of microglia from OHSC in the respective groups (Clo, n = 4; NMDA + Clo, n = 16; NMDA + Clo + MMF, n = 19). Additional application of MMF in this period led to reduction of PI positive degenerated neurons in microglia depleted OHSC (NMDA + Clo + MMF). Depletion of microglia led to an increase in number of damaged cells (NMDA + Clo, NMDA + Clo + MMF). Quantitative analyses of the mean numbers of panel (C) PI positive degenerating neurons (* p < 0.05 vs. NMDA or NMDA + Clo) and (D) IB 4 positive microglia (* p < 0.05 vs. NMDA). The asterisk denotes significant results regarding the respective measurement indicated with the bar. The values are served as a mean with standard error of the mean. Scale bars = 50 μm.

    Article Snippet: Microglia were labeled with fluorescein isothiocyanate (FICT)-conjugated isolectin B 4 (IB 4 , Vector Laboratories, Burlingame, CA, United States) diluted 1:50 in PBS/Triton for 24 h. Before staining, the OHSC were incubated in normal goat serum (NGS, Sigma Aldrich, 1:20) in PB (0.2 M) for 30 min.

    Techniques: Staining, Labeling, Incubation

    Qualitative images highlighting a few of the key anatomical differences between the fast twitch extensor digitorum longus (EDL) and slow twitch soleus muscles (A). From left to right: (Left) Vessel density images are z-projections of Dylight 594 conjugated lectin; (Middle) BODIPY images are z-projections of BODIPY positive lipid droplets. Red signal in BODIPY images are lectin stained blood vessels. Blue signal in BODIPY images are myonuclei. Arrows indicate BODIPY positive lipid droplets in zoomed image inlays; (Right) Mitochondrial NAD(P)H images are optical sections of reduced pyridine nucleotide autofluorescence in live isolated skeletal muscle. NAD(P)H fluorescence intensity is mapped onto the image (highest intensity in white). Scale bars are 25um.

    Journal: bioRxiv

    Article Title: Effects of fasting induced carbohydrate depletion on murine ischemic skeletal muscle function

    doi: 10.1101/846774

    Figure Lengend Snippet: Qualitative images highlighting a few of the key anatomical differences between the fast twitch extensor digitorum longus (EDL) and slow twitch soleus muscles (A). From left to right: (Left) Vessel density images are z-projections of Dylight 594 conjugated lectin; (Middle) BODIPY images are z-projections of BODIPY positive lipid droplets. Red signal in BODIPY images are lectin stained blood vessels. Blue signal in BODIPY images are myonuclei. Arrows indicate BODIPY positive lipid droplets in zoomed image inlays; (Right) Mitochondrial NAD(P)H images are optical sections of reduced pyridine nucleotide autofluorescence in live isolated skeletal muscle. NAD(P)H fluorescence intensity is mapped onto the image (highest intensity in white). Scale bars are 25um.

    Article Snippet: For microvascular imaging, Dylight 594 conjugated Griffonia simplificolia isolectin B 4 (GS-IB 4 ) (Vector Labs, Burlingame, CA) was injected retro-orbitally one hour prior to sacrifice.

    Techniques: Staining, Isolation, Fluorescence

    Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected Dylight594–conjugated Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).

    Journal: The American Journal of Pathology

    Article Title: Strain-Dependent Variation in Acute Ischemic Muscle Injury

    doi: 10.1016/j.ajpath.2018.01.008

    Figure Lengend Snippet: Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected Dylight594–conjugated Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).

    Article Snippet: Perfused Vessel Labeling Immediately after HLI surgery, 50 μL of 1 mg/mL Griffonia simplicolia isolectin-B 4 Dylight594 conjugate (Vector Labs, Burlingame, CA) was injected into the left retro-orbital sinus using a 31-gauge needle.

    Techniques: Immunofluorescence, Injection