gsk3 β (Cell Signaling Technology Inc)


Structured Review

Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsk3 β/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis"
Article Title: CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis
Journal: eLife
doi: 10.7554/eLife.86689

Figure Legend Snippet: ( A ) The relative protein levels of CRB3, GSK3-β, and β-catenin in CRB3-depleted MFC10A cells (normalized to β-actin). ( B ) The relative protein levels of CRB3, GSK3-β, and β-catenin in MDA-MB-231 and HCC 1806 breast cancer cells with CRB3b overexpression (normalized to β-actin). The data are three independent experiments performed in triplicate. Bars represent means ± SD; unpaired Student’s t -test, **p<0.01, ***p<0.001.
Techniques Used: Over Expression
gsk3 α β p ser21 ser9 (Cell Signaling Technology Inc)


Structured Review
Gsk3 α β P Ser21 Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsk3 α β p ser21 ser9/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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gsk3 β (Cell Signaling Technology Inc)


Structured Review
Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsk3 β/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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pi3k akt gsk3 (Cell Signaling Technology Inc)


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Pi3k Akt Gsk3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pi3k akt gsk3/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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p glycogen synthase kinase 3 gsk3 α β (Cell Signaling Technology Inc)


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P Glycogen Synthase Kinase 3 Gsk3 α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p glycogen synthase kinase 3 gsk3 α β/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "γ-aminobutyric acid B2 receptor: A potential therapeutic target for cholangiocarcinoma in patients with diabetes mellitus"
Article Title: γ-aminobutyric acid B2 receptor: A potential therapeutic target for cholangiocarcinoma in patients with diabetes mellitus
Journal: World Journal of Gastroenterology
doi: 10.3748/wjg.v29.i28.4416

Figure Legend Snippet: Baclofen inhibits the glycogen synthase kinase 3/β-catenin and signal transducer and activator of transcription 3 pathways. A and B: Phospho-kinase arrays reveal the inhibition of phosphorylation in multiple kinases and signal transducers; C: Glycogen synthase kinase 3 (GSK3)/β-catenin and signal transducer and activator of transcription 3 (STAT3) are included for further analysis as they are key pathways in cholangiocarcinoma (CCA) progression in which β-catenin and STAT3 are common targets of GSK3. RNA expression of γ-aminobutyric acid B2 receptor and that of STAT3 are significantly correlated in clinical CCA samples from Thai patients. HG: High glucose; GABBR2: γ-aminobutyric acid B2 receptor; GSK3: Glycogen synthase kinase 3; STAT3: Signal transducer and activator of transcription 3.
Techniques Used: Inhibition, RNA Expression

Figure Legend Snippet: Baclofen suppresses the glycogen synthase kinase 3/β-catenin and signal transducer and activators of transcription 3 pathways. A and B: Phosphorylation of glycogen synthase kinase 3 (GSK3) and signal transducer and activators of transcription 3 is decreased after baclofen treatment in both cholangiocarcinoma cell lines, both cultured in normal glucose and high glucose conditions. Total β-catenin protein is also decreased consistently with the decreased phosphorylated GSK3α/β. Western blots show the representative of three biological replications with the same trends of results. Band intensities are the average of three biological replications which are normalized using the intensities of glyceraldehyde-3-phosphate dehydrogenase for each experiment. The levels of phosphorylated forms are normalized with the total forms of their corresponding proteins. NG: Normal glucose; HG: High glucose; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GSK3: Glycogen synthase kinase 3; STAT3: Signal transducer and activator of transcription 3.
Techniques Used: Cell Culture, Western Blot

Figure Legend Snippet: Schematic summary of the effects of high glucose on γ-aminobutyric acid B2 receptor expression and the effects of baclofen on cholangiocarcinoma cells. High glucose induces the expression of γ-aminobutyric acid B2 receptor (GABBR2) in cholangiocarcinoma (CCA) cells. The treatment of baclofen, a GABBR2 agonist, to CCA cells inhibits phosphorylation of glycogen synthase kinase 3, resulting in the activation of the kinase activity which further phosphorylates β-catenin. Phosphorylated β-catenin is subjected to degradation preventing its function on promoting cell proliferation via c-Myc and cyclin D1 expression. On the other hand, activated GABBR2 by baclofen also inhibits phosphorylation of signal transducer and activator of transcription 3 (STAT3). The inhibition of STAT3 phosphorylation also suppresses its functions as a transcription factor for c-Myc and cyclin D1 expression. Activating GABBR2 by baclofen, thus, suppresses the proliferation of CCA cells. GABBR2: γ-aminobutyric acid B2 receptor; GSK3: Glycogen synthase kinase 3; STAT3: Signal transducer and activator of transcription 3.
Techniques Used: Expressing, Activation Assay, Activity Assay, Inhibition
gsk3 expression levels (Cell Signaling Technology Inc)


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Gsk3 Expression Levels, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsk3 expression levels/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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sirnas against gsk3 (Cell Signaling Technology Inc)


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Sirnas Against Gsk3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirnas against gsk3/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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total t gsk3 β (Cell Signaling Technology Inc)


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Total T Gsk3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total t gsk3 β/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Toward countering muscle and bone loss with spaceflight: GSK3 as a potential target"
Article Title: Toward countering muscle and bone loss with spaceflight: GSK3 as a potential target
Journal: iScience
doi: 10.1016/j.isci.2023.107047

Figure Legend Snippet: The combined effect of spaceflight across all missions on GSK3 content, phosphorylation status and MHC isoform content in soleus muscles (A) Total GSK3β content. (B) Phosphorylated GSK3β (Ser9) relative to total GSK3β content. (C) Total GSK3α content. (D) Phosphorylated GSK3α (Ser21) relative to total GSK3β content. (E) MHC I content. (F) MHC IIa content. (G) MHC IIx content. (H) MHC IIb content. Fold-change (FC) data were calculated for each mission by dividing the Flight group by the average of the combined GC and VIV groups. The combined dataset represents the mean ± SEM and p value (Student’s t test) for all FC data (RR1, RR9, RR18, and BION-M1).
Techniques Used:

Figure Legend Snippet: Partial muscle-specific Gsk3 knockdown (GSK3 mKD ) increases soleus muscle mass, myogenic signaling, and the oxidative phenotype while preserving muscle strength after 7 days of hindlimb suspension (HLS) (A–C) DXA scan analyses showing that GSK3 mKD mice have no change in body mass but have lowered % fat mass and increased % lean mass even after 7 days of HLS. (D and E) Absolute and relative (to body mass) soleus muscle weights. (F and G) Percent reduction of absolute and relative soleus muscle weights in GSK3 mKD and GSK3 flox mice when compared to their respective mobile controls (see
Techniques Used: Preserving, Staining, Western Blot

Figure Legend Snippet: GSK3β phosphorylation and content in femur samples obtained from male RR9 mice (A and B) Bone mineral content (BMC) and bone mineral density (BMD) of the individual bones obtained from a small animal DXA scanner. (C) Representative western blot images of phosphorylated (Ser9) and total GSK3β. (D and E) Western blot analysis of phosphorylated (Ser9) and total GSK3β content normalized to ponceau. (F) GSK3 activation status measured as the ratio of phosphorylated (Ser9) GSK3β relative to total GSK3β. (G) Representative DXA scan showing region-specific analysis of the femur, tibia, and lumbar spine in mice. (H) Region-specific BMD analysis in mobile GSK3 mKD and GSK3 flox mice measured at baseline. (I) Region-specific BMD analysis in mobile GSK3 mKD and GSK3 flox mice measured after 7 days of HLS. (J) Western blot analysis of soleus muscle FNDC5 from GSK3 mKD and GSK3 flox mice measured after 7 days of HLS. (K) Proposed tissue crosstalk between muscle and bone with muscle-specific Gsk3 deletion leading to an increase in FNDC5 and tibia BMD. ∗p < 0.05, ∗∗∗p < 0.001 using a Student’s t test (n = 6–12 per group for (A–F); n = 3–5 per group for (H–J). For (A–F), GC and VIV controls were combined to increase statistical power. All values are presented as means ± SEM.
Techniques Used: Western Blot, Activation Assay
gsk 3 β (Cell Signaling Technology Inc)


Structured Review
Gsk 3 β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsk 3 β/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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gsk 3 β 27c10 (Cell Signaling Technology Inc)


Structured Review
Gsk 3 β 27c10, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsk 3 β 27c10/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99