gsk 126  (TargetMol)

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Chemical structure, molecular formulae and average molecular weights of abemaciclib, GSK126, tazemetostat and the internal standard palbociclib.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques:

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Linear binary gradient for the analysis of abemaciclib, GSK126 and tazemetostat (eluent A: water with 0.1% formic acid; eluent B: acetonitrile with 0.1% formic acid).

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques:

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Mass spectrometric parameters for the detection of tazemetostat, abemaciclib, GSK126 and palbociclib.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques:

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Calibration range and quality control level concentrations of abemaciclib, GSK126 and tazemetostat.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques: Control, Concentration Assay

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Representative chromatograms of cell suspensions in 20 mM Tris buffer, pH 7.4, spiked with blank methanol ( A ), only palbociclib (IS) ( B ) or palbociclib (IS) and the three analytes (abemaciclib, GSK126, tazemetostat) at lower limit of quantification (LLOQ, ( C )) or upper limit of quantification (ULOQ, ( D )). The mass transition that applies to the respective line of chromatograms is listed on the left.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques:

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Representative calibration curves of abemaciclib, GSK126 and tazemetostat.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques:

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Within-run and between-run accuracy and precision.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques: Control

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Stability of abemaciclib, GSK126 and tazemetostat.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques: Control

Journal: Pharmaceutics

Article Title: Validation of an LC-MS/MS Method for the Simultaneous Intracellular Quantification of the CDK4/6 Inhibitor Abemaciclib and the EZH2 Inhibitors GSK126 and Tazemetostat

doi: 10.3390/pharmaceutics17040433

Figure Lengend Snippet: Intracellular concentrations of abemaciclib (A), GSK126 (G) and tazemetostat (T) in GBM06 and GBM15 cells. Cells were incubated with either 1 µM abemaciclib, 10 µM GSK126, 10 µM tazemetostat or a combination of 1 µM abemaciclib plus 10 µM GSK126 or 10 µM tazemetostat for 72 h, followed by lysis and LC-MS/MS analysis of intracellular drug concentrations. Data are mean + SD of n = 4 independent experiments.

Article Snippet: Abemaciclib and GSK126 were ordered from LKT Laboratories Inc. (St. Paul, MN, USA).

Techniques: Incubation, Lysis, Liquid Chromatography with Mass Spectroscopy