Journal: BMC Genomics
Article Title: Reorganization of metastamiRs in the evolution of metastatic aggressive neuroblastoma cells
Figure Lengend Snippet: MetastamiRs dependent regulation of functional protein targets. a Operetta high-content confocal imaging showing immunofluorescence of ADAMTS-1, ASK-1, CREB, FOSB, GRB10, kRAS, MMP2, MMP3/10, p38, AKT, PTPN3, STAT3, TNFα and VEGFa in MSDACs. b Histograms of mean cell–Alexa Fluor intensity obtained from Columbus automated batch analysis showing alterations in the expression ( i ) GRB10, MMP2, p38, STAT3, TNFα and VEGF in cells with hsa-miR-125b mimic, ( ii ) EGFR FOSB, kRAS, p38, PTPN3 and VEGF in hsa-miR-27b mimic transfected cells, ( iii ) ASK1, CREB, MMP2, MMP3/10, PTPN3, STAT3and VEGF in MSDACs with hsa-miR-20a mimic and, ( iv ) MMP2, MMP3/10, PTPN3 and STAT3 with hsa-miR-93 mimic in MSDACs. c Histograms of mean cell–Alexa Fluor intensity showing alterations in the expression of ( i ) ADAMTS-1 and CREB with hsa-miR-1224-3p inhibition and, ( ii ) ADAMTS-1, ASK1, FOSB and AKT-1 with hsa-miR-1260 inhibition. Group-wise comparisons were performed with ANOVA with Tukey’s post-hoc correction
Article Snippet: Core-specific metadata for the TMA was exported to excel and the group-wise comparisons were performed with two-way ANOVA with Tukey’s post-hoc correction (GraphPad Prism).
Techniques: Functional Assay, Imaging, Immunofluorescence, Expressing, Transfection, Inhibition