griffonia simplicifolia lectin gsl i isolectin b4 conjugated  (Vector Laboratories)


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    Vector Laboratories griffonia simplicifolia lectin gsl i isolectin b4 conjugated
    Griffonia Simplicifolia Lectin Gsl I Isolectin B4 Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    griffonia simplicifolia lectin i isolectin b4  (Vector Laboratories)


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    Vector Laboratories griffonia simplicifolia lectin i isolectin b4
    Griffonia Simplicifolia Lectin I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dylight 649 conjugated griffonia simplicifolia lectin i isolectin b4  (Vector Laboratories)


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    Vector Laboratories dylight 649 conjugated griffonia simplicifolia lectin i isolectin b4
    AAV-HSA-mCAT expression does not impact central and peripheral hemodynamics or hindlimb muscle capillarization following AVF surgery. ( A ) Doppler ultrasound assessment of systemic hemodynamics captured at infrarenal aorta and inferior vena cava at POD10 (N = 5–10/group/sex). Dotted lines represent vessel diameters and flow velocities in mice without an AVF from our previous study . ( B ) Blood perfusion of the ventral paw immediately post-operatively, and at POD3 and POD13 (N = 8–10/group/sex). ( C ) Representative images of muscle labeled for capillaries using Griffonia <t>Simplicifolia</t> isolectin. Images were from male mice. ( D ) Capillary density of tibialis anterior, EDL, and soleus muscles (N = 8–10/group/sex). Data in panel ( B ) were analyzed using mixed-effects analysis. All other data were analyzed using a two-way ANOVA with Šidák’s post-hoc testing. Values are mean ± SD.
    Dylight 649 Conjugated Griffonia Simplicifolia Lectin I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Mitochondrial targeted catalase improves muscle strength following arteriovenous fistula creation in mice with chronic kidney disease"

    Article Title: Mitochondrial targeted catalase improves muscle strength following arteriovenous fistula creation in mice with chronic kidney disease

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-58805-1

    AAV-HSA-mCAT expression does not impact central and peripheral hemodynamics or hindlimb muscle capillarization following AVF surgery. ( A ) Doppler ultrasound assessment of systemic hemodynamics captured at infrarenal aorta and inferior vena cava at POD10 (N = 5–10/group/sex). Dotted lines represent vessel diameters and flow velocities in mice without an AVF from our previous study . ( B ) Blood perfusion of the ventral paw immediately post-operatively, and at POD3 and POD13 (N = 8–10/group/sex). ( C ) Representative images of muscle labeled for capillaries using Griffonia Simplicifolia isolectin. Images were from male mice. ( D ) Capillary density of tibialis anterior, EDL, and soleus muscles (N = 8–10/group/sex). Data in panel ( B ) were analyzed using mixed-effects analysis. All other data were analyzed using a two-way ANOVA with Šidák’s post-hoc testing. Values are mean ± SD.
    Figure Legend Snippet: AAV-HSA-mCAT expression does not impact central and peripheral hemodynamics or hindlimb muscle capillarization following AVF surgery. ( A ) Doppler ultrasound assessment of systemic hemodynamics captured at infrarenal aorta and inferior vena cava at POD10 (N = 5–10/group/sex). Dotted lines represent vessel diameters and flow velocities in mice without an AVF from our previous study . ( B ) Blood perfusion of the ventral paw immediately post-operatively, and at POD3 and POD13 (N = 8–10/group/sex). ( C ) Representative images of muscle labeled for capillaries using Griffonia Simplicifolia isolectin. Images were from male mice. ( D ) Capillary density of tibialis anterior, EDL, and soleus muscles (N = 8–10/group/sex). Data in panel ( B ) were analyzed using mixed-effects analysis. All other data were analyzed using a two-way ANOVA with Šidák’s post-hoc testing. Values are mean ± SD.

    Techniques Used: Expressing, Labeling, Muscles

    griffonia simplicifolia lectin gsl isolectin b4  (Vector Laboratories)


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    Vector Laboratories griffonia simplicifolia lectin gsl isolectin b4
    Griffonia Simplicifolia Lectin Gsl Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dylight 649 conjugated griffonia simplicifolia lectin i isolectin b4  (Vector Laboratories)


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    Vector Laboratories dylight 649 conjugated griffonia simplicifolia lectin i isolectin b4
    Dylight 649 Conjugated Griffonia Simplicifolia Lectin I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated griffonia simplicifolia isolectin b4  (Vector Laboratories)


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    Vector Laboratories biotinylated griffonia simplicifolia isolectin b4
    SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with <t>isolectin-B4</t> (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).
    Biotinylated Griffonia Simplicifolia Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Bystander activation of microglia by Brucella abortus -infected astrocytes induces neuronal death via IL-6 trans-signaling"

    Article Title: Bystander activation of microglia by Brucella abortus -infected astrocytes induces neuronal death via IL-6 trans-signaling

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2023.1343503

    SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with isolectin-B4 (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).
    Figure Legend Snippet: SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with isolectin-B4 (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).

    Techniques Used: Infection, Labeling, Fluorescence, Microscopy

    anti griffonia simplicifolia isolectin b4  (Vector Laboratories)


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    Vector Laboratories anti griffonia simplicifolia isolectin b4
    Anti Griffonia Simplicifolia Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti griffonia simplicifolia isolectin b4  (Vector Laboratories)


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    Vector Laboratories anti griffonia simplicifolia isolectin b4
    Anti Griffonia Simplicifolia Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    griffonia simplicifolia lectin gsl isolectin b4  (Vector Laboratories)


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    Vector Laboratories griffonia simplicifolia lectin gsl isolectin b4
    Griffonia Simplicifolia Lectin Gsl Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    blood vessel marker griffonia simplicifolia biotinylated isolectin b4  (Vector Laboratories)


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    Vector Laboratories blood vessel marker griffonia simplicifolia biotinylated isolectin b4
    Blood Vessel Marker Griffonia Simplicifolia Biotinylated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories griffonia simplicifolia lectin gsl i isolectin b4 conjugated
    Griffonia Simplicifolia Lectin Gsl I Isolectin B4 Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AAV-HSA-mCAT expression does not impact central and peripheral hemodynamics or hindlimb muscle capillarization following AVF surgery. ( A ) Doppler ultrasound assessment of systemic hemodynamics captured at infrarenal aorta and inferior vena cava at POD10 (N = 5–10/group/sex). Dotted lines represent vessel diameters and flow velocities in mice without an AVF from our previous study . ( B ) Blood perfusion of the ventral paw immediately post-operatively, and at POD3 and POD13 (N = 8–10/group/sex). ( C ) Representative images of muscle labeled for capillaries using Griffonia <t>Simplicifolia</t> isolectin. Images were from male mice. ( D ) Capillary density of tibialis anterior, EDL, and soleus muscles (N = 8–10/group/sex). Data in panel ( B ) were analyzed using mixed-effects analysis. All other data were analyzed using a two-way ANOVA with Šidák’s post-hoc testing. Values are mean ± SD.
    Dylight 649 Conjugated Griffonia Simplicifolia Lectin I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AAV-HSA-mCAT expression does not impact central and peripheral hemodynamics or hindlimb muscle capillarization following AVF surgery. ( A ) Doppler ultrasound assessment of systemic hemodynamics captured at infrarenal aorta and inferior vena cava at POD10 (N = 5–10/group/sex). Dotted lines represent vessel diameters and flow velocities in mice without an AVF from our previous study . ( B ) Blood perfusion of the ventral paw immediately post-operatively, and at POD3 and POD13 (N = 8–10/group/sex). ( C ) Representative images of muscle labeled for capillaries using Griffonia <t>Simplicifolia</t> isolectin. Images were from male mice. ( D ) Capillary density of tibialis anterior, EDL, and soleus muscles (N = 8–10/group/sex). Data in panel ( B ) were analyzed using mixed-effects analysis. All other data were analyzed using a two-way ANOVA with Šidák’s post-hoc testing. Values are mean ± SD.
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    SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with <t>isolectin-B4</t> (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).
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    SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with <t>isolectin-B4</t> (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).
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    SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with <t>isolectin-B4</t> (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).
    Blood Vessel Marker Griffonia Simplicifolia Biotinylated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AAV-HSA-mCAT expression does not impact central and peripheral hemodynamics or hindlimb muscle capillarization following AVF surgery. ( A ) Doppler ultrasound assessment of systemic hemodynamics captured at infrarenal aorta and inferior vena cava at POD10 (N = 5–10/group/sex). Dotted lines represent vessel diameters and flow velocities in mice without an AVF from our previous study . ( B ) Blood perfusion of the ventral paw immediately post-operatively, and at POD3 and POD13 (N = 8–10/group/sex). ( C ) Representative images of muscle labeled for capillaries using Griffonia Simplicifolia isolectin. Images were from male mice. ( D ) Capillary density of tibialis anterior, EDL, and soleus muscles (N = 8–10/group/sex). Data in panel ( B ) were analyzed using mixed-effects analysis. All other data were analyzed using a two-way ANOVA with Šidák’s post-hoc testing. Values are mean ± SD.

    Journal: Scientific Reports

    Article Title: Mitochondrial targeted catalase improves muscle strength following arteriovenous fistula creation in mice with chronic kidney disease

    doi: 10.1038/s41598-024-58805-1

    Figure Lengend Snippet: AAV-HSA-mCAT expression does not impact central and peripheral hemodynamics or hindlimb muscle capillarization following AVF surgery. ( A ) Doppler ultrasound assessment of systemic hemodynamics captured at infrarenal aorta and inferior vena cava at POD10 (N = 5–10/group/sex). Dotted lines represent vessel diameters and flow velocities in mice without an AVF from our previous study . ( B ) Blood perfusion of the ventral paw immediately post-operatively, and at POD3 and POD13 (N = 8–10/group/sex). ( C ) Representative images of muscle labeled for capillaries using Griffonia Simplicifolia isolectin. Images were from male mice. ( D ) Capillary density of tibialis anterior, EDL, and soleus muscles (N = 8–10/group/sex). Data in panel ( B ) were analyzed using mixed-effects analysis. All other data were analyzed using a two-way ANOVA with Šidák’s post-hoc testing. Values are mean ± SD.

    Article Snippet: After multiple washes with 1× PBS, sections were stained with a secondary antibody in blocking buffer as follows: for mice treated with AAV-HSA-GFP were labeled with Alexa Fluor 488 goat anti-rabbit IgG (1:250, ThermoFisher Scientific, Cat. No. A-11008), whereas mice treated with AAV-HSA-mCAT were labeled with Alexa Fluor 555 goat anti-rabbit IgG (1:250, ThermoFisher Scientific, Cat. No. A-21428), and both were labeled with Dylight 649-conjugated Griffonia Simplicifolia Lectin I Isolectin B4 (Vector Laboratories, Cat. No. DL-1208) to visualize capillaries.

    Techniques: Expressing, Labeling, Muscles

    SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with isolectin-B4 (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).

    Journal: Frontiers in Immunology

    Article Title: Bystander activation of microglia by Brucella abortus -infected astrocytes induces neuronal death via IL-6 trans-signaling

    doi: 10.3389/fimmu.2023.1343503

    Figure Lengend Snippet: SN from B. abortus -infected astrocytes induce neuronal death in co-cultures of neurons/microglia. Neurons/microglia co-cultures were stimulated with SN from non-infected (NI) or B. abortus -infected ( Ba ) astrocytes (Astr SN) diluted in complete medium (1/2) or left untreated (control) for 24 h or 48 h (A) , or with different dilutions of SN from infected astrocytes for 48 h (B) . Representative images from neurons/microglia co-cultures showing neurons labeled with anti-β-Tubulin III antibody (red) and microglia labeled with isolectin-B4 (green). Scale bar: 50 μm (C) . The percentage (%) of neuronal density was evaluated in five independent experiments using neurons, microglia, and astrocytes SN from different animals (D) . Astrocytes SN were ultracentrifuged (OMVs-free SN) or not and used to stimulate neurons/microglia co-cultures for 48 h (E) . Cultures of neurons with microglia or neurons alone were treated with SN from non-infected or B. abortus -infected astrocytes for 48 h and neuronal density was evaluated (F) . The density of neurons was evaluated by fluorescence microscopy. The percentage (%) of viable and apoptotic neurons was calculated vs. control condition. Data are shown as mean ± SEM from a representative experiment of three performed, except where indicated. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 vs. control condition, except where indicated. Non-significant (ns).

    Article Snippet: Microglia were labeled with biotinylated Griffonia simplicifolia isolectin-B4 (1:500 dilution; Vector Laboratories) followed by Alexa Fluor 488-labeled streptavidin (1:200 dilution; BioLegend).

    Techniques: Infection, Labeling, Fluorescence, Microscopy