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1) Product Images from "Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells"
Article Title: Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells
Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Figure Legend Snippet: Quantitative effect of rBC2LCN-PE38 on the cell viability of human induced pluripotent stem cells (hiPSCs). 201B7 hiPSCs, 253G1 hiPSCs, human dermal fibroblasts (hFibs), and human adipose-derived mesenchymal stem cells (hMSCs) were cultured with varying concentrations of rBC2LCN-PE38 or rBC2LCN-PE23. After 24 h, the medium was replaced with fresh medium supplemented with 20 μL of Cell Counting Kit (CCK)-8 solution, and the absorbance at 450 nm was measured after a further 4 h incubation. Viability (%) was calculated from the absorbance at 450 nm of treated cells relative to untreated cells. Data are shown as mean ± SD (for triplicate measurements). Median lethal dose (LC50) was calculated by GraphPad Prism7 software.
Techniques Used: Derivative Assay, Cell Culture, Cell Counting, CCK-8 Assay, Incubation, Software