Structured Review

Graph Pad Software Inc graphpad prism7 software
Quantitative effect of rBC2LCN-PE38 on the cell viability of human induced pluripotent stem cells (hiPSCs). 201B7 hiPSCs, 253G1 hiPSCs, human dermal fibroblasts (hFibs), and human adipose-derived mesenchymal stem cells (hMSCs) were cultured with varying concentrations of rBC2LCN-PE38 or rBC2LCN-PE23. After 24 h, the medium was replaced with fresh medium supplemented with 20 μL of Cell Counting Kit (CCK)-8 solution, and the absorbance at 450 nm was measured after a further 4 h incubation. Viability (%) was calculated from the absorbance at 450 nm of treated cells relative to untreated cells. Data are shown as mean ± SD (for triplicate measurements). Median lethal dose (LC50) was calculated by <t>GraphPad</t> <t>Prism7</t> software.
Graphpad Prism7 Software, supplied by Graph Pad Software Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/graphpad prism7 software/product/Graph Pad Software Inc
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
graphpad prism7 software - by Bioz Stars, 2022-01
99/100 stars

Images

1) Product Images from "Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells"

Article Title: Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules22071151

Quantitative effect of rBC2LCN-PE38 on the cell viability of human induced pluripotent stem cells (hiPSCs). 201B7 hiPSCs, 253G1 hiPSCs, human dermal fibroblasts (hFibs), and human adipose-derived mesenchymal stem cells (hMSCs) were cultured with varying concentrations of rBC2LCN-PE38 or rBC2LCN-PE23. After 24 h, the medium was replaced with fresh medium supplemented with 20 μL of Cell Counting Kit (CCK)-8 solution, and the absorbance at 450 nm was measured after a further 4 h incubation. Viability (%) was calculated from the absorbance at 450 nm of treated cells relative to untreated cells. Data are shown as mean ± SD (for triplicate measurements). Median lethal dose (LC50) was calculated by GraphPad Prism7 software.
Figure Legend Snippet: Quantitative effect of rBC2LCN-PE38 on the cell viability of human induced pluripotent stem cells (hiPSCs). 201B7 hiPSCs, 253G1 hiPSCs, human dermal fibroblasts (hFibs), and human adipose-derived mesenchymal stem cells (hMSCs) were cultured with varying concentrations of rBC2LCN-PE38 or rBC2LCN-PE23. After 24 h, the medium was replaced with fresh medium supplemented with 20 μL of Cell Counting Kit (CCK)-8 solution, and the absorbance at 450 nm was measured after a further 4 h incubation. Viability (%) was calculated from the absorbance at 450 nm of treated cells relative to untreated cells. Data are shown as mean ± SD (for triplicate measurements). Median lethal dose (LC50) was calculated by GraphPad Prism7 software.

Techniques Used: Derivative Assay, Cell Culture, Cell Counting, CCK-8 Assay, Incubation, Software

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Graph Pad Software Inc prism version 7 0
    Inhibition studies of five potential hPepT1 substrates on the uptake of GlySar. (A) 10 µM of  3 H-GlySar was incubated with a series concentration of inhibitors at room temperature for 2 min, the percent uptake calculated (relative to control), and the results then fit to a dose-dependent inhibition model using Prism version 7.0 (mean ± SE,  n  = 3). (B) Experimentally confirmed ligands with (a) compound referring to the name of the compound; (b) 2D Sketch representing the two-dimensional structure of the compound; (c) IC 50  referring to the experimental IC 50 .
    Prism Version 7 0, supplied by Graph Pad Software Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prism version 7 0/product/Graph Pad Software Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prism version 7 0 - by Bioz Stars, 2022-01
    99/100 stars
      Buy from Supplier

    99
    Graph Pad Software Inc graphpad prism software
    Survival curves of N. lugens 1–7 d after injection with dsRNA of Nan , Iav or GFP , when fed on rice (A and B). (A) A log-rank (Mantel-Cox) test using <t>GraphPad</t> Prism software showed a significant difference (*) in survival rate between ds GFP and ds Nan treatments ( P
    Graphpad Prism Software, supplied by Graph Pad Software Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/graphpad prism software/product/Graph Pad Software Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    graphpad prism software - by Bioz Stars, 2022-01
    99/100 stars
      Buy from Supplier

    86
    Graph Pad Software Inc one phase exponential decay equation
    Association versus time plots for specific binding of [ 177 Lu]Lu-OPS201 ( A ) and [ 177 Lu]Lu-DOTA-TATE ( C ) to HEK-SST 2 cell membranes (10 µg/well). Membranes were incubated with the indicated concentrations of each radioligand for up to 60 min. Data were fitted using the <t>one-phase</t> <t>exponential</t> association <t>equation.</t> Saturation binding profile for [ 177 Lu]Lu-OPS201 ( B ) and [ 177 Lu]Lu-DOTA-TATE ( D ). Presented binding values refer to the average of the 20–60 min “equilibrium data” for each radioligand concentration.
    One Phase Exponential Decay Equation, supplied by Graph Pad Software Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one phase exponential decay equation/product/Graph Pad Software Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    one phase exponential decay equation - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition studies of five potential hPepT1 substrates on the uptake of GlySar. (A) 10 µM of  3 H-GlySar was incubated with a series concentration of inhibitors at room temperature for 2 min, the percent uptake calculated (relative to control), and the results then fit to a dose-dependent inhibition model using Prism version 7.0 (mean ± SE,  n  = 3). (B) Experimentally confirmed ligands with (a) compound referring to the name of the compound; (b) 2D Sketch representing the two-dimensional structure of the compound; (c) IC 50  referring to the experimental IC 50 .

    Journal: Molecular pharmaceutics

    Article Title: Chemical Modulation of the Human Oligopeptide Transporter 1, hPepT1

    doi: 10.1021/acs.molpharmaceut.7b00775

    Figure Lengend Snippet: Inhibition studies of five potential hPepT1 substrates on the uptake of GlySar. (A) 10 µM of 3 H-GlySar was incubated with a series concentration of inhibitors at room temperature for 2 min, the percent uptake calculated (relative to control), and the results then fit to a dose-dependent inhibition model using Prism version 7.0 (mean ± SE, n = 3). (B) Experimentally confirmed ligands with (a) compound referring to the name of the compound; (b) 2D Sketch representing the two-dimensional structure of the compound; (c) IC 50 referring to the experimental IC 50 .

    Article Snippet: The unknown parameters (IC50 and s ) were estimated by nonlinear regression (weighting of unity) using Prism version 7.0 (GraphPad Software, Inc., La Jolla, CA).

    Techniques: Inhibition, Incubation, Concentration Assay

    Mucosal colonization and lesion scores in piglets. (A) Colonization scores; (B) lesion scores. Mucosal colonization was scored from 0 to 5 (0 = no infection detected; 1 = 1%–20% epithelial surface infected; 2 = 21%–40% surface infected; 3 = 41%–60% surface infected; 4 = 61%–80% surface infected; 5 = 81%–100% surface infected) in 3 fields from each anatomic site: duodenum, jejunum, ileum, cecum, and spiral colon and then averaged. For each piglet, the final score is a sum of the averages (A). Mucosal lesion scores were generated for each site for changes in villi, epithelial cells, and inflammation. The following scores were used: normal = 0, equivocal = 2.5, mild = 5, moderate = 10, marked = 15. The final lesion score was a sum of scores from duodenum, jejunum, ileum, cecum, and colon for each piglet. The scatter plot (A and B) represents mean ± standard error of the mean. A Mann-Whitney test was conducted using GraphPad Prism 7.03: *, P

    Journal: The Journal of Infectious Diseases

    Article Title: Piperazine-Derivative MMV665917: An Effective Drug in the Diarrheic Piglet Model of Cryptosporidium hominis

    doi: 10.1093/infdis/jiz105

    Figure Lengend Snippet: Mucosal colonization and lesion scores in piglets. (A) Colonization scores; (B) lesion scores. Mucosal colonization was scored from 0 to 5 (0 = no infection detected; 1 = 1%–20% epithelial surface infected; 2 = 21%–40% surface infected; 3 = 41%–60% surface infected; 4 = 61%–80% surface infected; 5 = 81%–100% surface infected) in 3 fields from each anatomic site: duodenum, jejunum, ileum, cecum, and spiral colon and then averaged. For each piglet, the final score is a sum of the averages (A). Mucosal lesion scores were generated for each site for changes in villi, epithelial cells, and inflammation. The following scores were used: normal = 0, equivocal = 2.5, mild = 5, moderate = 10, marked = 15. The final lesion score was a sum of scores from duodenum, jejunum, ileum, cecum, and colon for each piglet. The scatter plot (A and B) represents mean ± standard error of the mean. A Mann-Whitney test was conducted using GraphPad Prism 7.03: *, P

    Article Snippet: We used GraphPad Prism 7.03 software (GraphPad Software, San Diego, CA) to perform graphing and statistical analyses; *, P < .05, **, P < .01, ***, P < .001, and ****, P< .0001 indicated levels of significance, as shown in the figures.

    Techniques: Infection, Generated, MANN-WHITNEY

    Alteration of serum biochemical and hematological data in piglets after MMV665917 treatment. Serum biochemical and hematological parameters were analyzed from blood samples of euthanized piglets. The scatter plots represent mean ± standard error of the mean. Tukey’s multiple comparisons test was conducted among 3 groups using GraphPad Prism 7.03. *, P

    Journal: The Journal of Infectious Diseases

    Article Title: Piperazine-Derivative MMV665917: An Effective Drug in the Diarrheic Piglet Model of Cryptosporidium hominis

    doi: 10.1093/infdis/jiz105

    Figure Lengend Snippet: Alteration of serum biochemical and hematological data in piglets after MMV665917 treatment. Serum biochemical and hematological parameters were analyzed from blood samples of euthanized piglets. The scatter plots represent mean ± standard error of the mean. Tukey’s multiple comparisons test was conducted among 3 groups using GraphPad Prism 7.03. *, P

    Article Snippet: We used GraphPad Prism 7.03 software (GraphPad Software, San Diego, CA) to perform graphing and statistical analyses; *, P < .05, **, P < .01, ***, P < .001, and ****, P< .0001 indicated levels of significance, as shown in the figures.

    Techniques:

    MMV665917 concentration in plasma and gut contents: (A) MMV665917 concentration in plasma after dosing with MMV665917 20 mg/kg or 10 mg/kg dose and (B) MMV665917 concentration in gut contents. Piglets were inoculated orally with Cryptosporidium hominis oocysts 2 days after birth and dosing with MMV665917 began 3 days postchallenge. Plasma samples were collected at 0 hour (before 1st dose), 2 hours (post 1st dose), 72 hours (12 hours post 6th dose), 74 hours (2 hours post 7th dose), 144 hours (12 hours post 12th dose), 146 hours (2 hours post 13th dose), and 216 hours (60 hours post 14th dose). Gut contents were also collected at 216 hours (60 hours post 14th dose). Unpaired t test was conducted using GraphPad Prism 7.03. EC 90 = 5.0 (2.5 to 13.3) μM.

    Journal: The Journal of Infectious Diseases

    Article Title: Piperazine-Derivative MMV665917: An Effective Drug in the Diarrheic Piglet Model of Cryptosporidium hominis

    doi: 10.1093/infdis/jiz105

    Figure Lengend Snippet: MMV665917 concentration in plasma and gut contents: (A) MMV665917 concentration in plasma after dosing with MMV665917 20 mg/kg or 10 mg/kg dose and (B) MMV665917 concentration in gut contents. Piglets were inoculated orally with Cryptosporidium hominis oocysts 2 days after birth and dosing with MMV665917 began 3 days postchallenge. Plasma samples were collected at 0 hour (before 1st dose), 2 hours (post 1st dose), 72 hours (12 hours post 6th dose), 74 hours (2 hours post 7th dose), 144 hours (12 hours post 12th dose), 146 hours (2 hours post 13th dose), and 216 hours (60 hours post 14th dose). Gut contents were also collected at 216 hours (60 hours post 14th dose). Unpaired t test was conducted using GraphPad Prism 7.03. EC 90 = 5.0 (2.5 to 13.3) μM.

    Article Snippet: We used GraphPad Prism 7.03 software (GraphPad Software, San Diego, CA) to perform graphing and statistical analyses; *, P < .05, **, P < .01, ***, P < .001, and ****, P< .0001 indicated levels of significance, as shown in the figures.

    Techniques: Concentration Assay

    Survival curves of N. lugens 1–7 d after injection with dsRNA of Nan , Iav or GFP , when fed on rice (A and B). (A) A log-rank (Mantel-Cox) test using GraphPad Prism software showed a significant difference (*) in survival rate between ds GFP and ds Nan treatments ( P

    Journal: Journal of Insect Science

    Article Title: Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata lugens (Hemiptera: Delphacidae)

    doi: 10.1093/jisesa/ieaa002

    Figure Lengend Snippet: Survival curves of N. lugens 1–7 d after injection with dsRNA of Nan , Iav or GFP , when fed on rice (A and B). (A) A log-rank (Mantel-Cox) test using GraphPad Prism software showed a significant difference (*) in survival rate between ds GFP and ds Nan treatments ( P

    Article Snippet: Insect survival curves were made with the Kaplan–Meier method and comparatively analyzed with a log-rank (Mantel-Cox) test using GraphPad Prism software (version 7.0; GraphPad Inc.).

    Techniques: Injection, Software

    Association versus time plots for specific binding of [ 177 Lu]Lu-OPS201 ( A ) and [ 177 Lu]Lu-DOTA-TATE ( C ) to HEK-SST 2 cell membranes (10 µg/well). Membranes were incubated with the indicated concentrations of each radioligand for up to 60 min. Data were fitted using the one-phase exponential association equation. Saturation binding profile for [ 177 Lu]Lu-OPS201 ( B ) and [ 177 Lu]Lu-DOTA-TATE ( D ). Presented binding values refer to the average of the 20–60 min “equilibrium data” for each radioligand concentration.

    Journal: Pharmaceuticals

    Article Title: Distinct In Vitro Binding Profile of the Somatostatin Receptor Subtype 2 Antagonist [177Lu]Lu-OPS201 Compared to the Agonist [177Lu]Lu-DOTA-TATE

    doi: 10.3390/ph14121265

    Figure Lengend Snippet: Association versus time plots for specific binding of [ 177 Lu]Lu-OPS201 ( A ) and [ 177 Lu]Lu-DOTA-TATE ( C ) to HEK-SST 2 cell membranes (10 µg/well). Membranes were incubated with the indicated concentrations of each radioligand for up to 60 min. Data were fitted using the one-phase exponential association equation. Saturation binding profile for [ 177 Lu]Lu-OPS201 ( B ) and [ 177 Lu]Lu-DOTA-TATE ( D ). Presented binding values refer to the average of the 20–60 min “equilibrium data” for each radioligand concentration.

    Article Snippet: All data were fitted according to the one-phase exponential decay equation (GraphPad Software Inc., Prism 7).

    Techniques: Binding Assay, Incubation, Concentration Assay