gram positive bacteria  (ATCC)


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    Structured Review

    ATCC gram positive bacteria
    Gram Positive Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Related Articles

    In Vitro:

    Article Title: Eco-Friendly Synthesis, Characterization and Biological Evaluation of Some Novel Pyrazolines Containing Thiazole Moiety as Potential Anticancer and Antimicrobial Agents
    Article Snippet: .. Evaluation of the Antimicrobial Activity The in vitro antimicrobial activities of the newly synthesized compounds and reference drugs were tested by inhibition zone technique [ , ] and minimum inhibitory concentration (MIC), using two fungi: Aspergillus fumigatus (RCMB 002008 (4) and Candida albicans (RCMB 05036), two gram-positive bacteria: Staphylococcus aureus (RCMB 010010), and Bacillus subtilis (RCMB 010067), two gram-negative bacteria: Escherichia coli (RCMB 010052), and Proteus vulgaris RCMB 004 (1) ATCC 13315, and the results are depicted in and . ..

    Synthesized:

    Article Title: Novel 5′-Norcarbocyclic Pyrimidine Derivatives as Antibacterial Agents
    Article Snippet: .. The antibacterial activity of the synthesized compounds was also evaluated against Gram-negative bacteria including Pseudomonas aeruginosa (P. aeruginosa ) ATCC strain 27853 and Escherichia coli (E. coli ) ATCC strain 25922, Gram-positive bacteria including Bacillus subtilis (B. subtilis) ATCC strain 6633, Staphylococcus aureus (S. aureus ) INA 00761 (MRSA) and Leuconostoc mesenteroides (L. mesenteroides ) VKPM B-4177. .. Mycobacterium smegmatis (M. smegmatis ) mc2 155 and M. smegmatis VKPM Ac 1339, were also used in the primary screening for potential antibiotic activity.

    Article Title: Eco-Friendly Synthesis, Characterization and Biological Evaluation of Some Novel Pyrazolines Containing Thiazole Moiety as Potential Anticancer and Antimicrobial Agents
    Article Snippet: .. Evaluation of the Antimicrobial Activity The in vitro antimicrobial activities of the newly synthesized compounds and reference drugs were tested by inhibition zone technique [ , ] and minimum inhibitory concentration (MIC), using two fungi: Aspergillus fumigatus (RCMB 002008 (4) and Candida albicans (RCMB 05036), two gram-positive bacteria: Staphylococcus aureus (RCMB 010010), and Bacillus subtilis (RCMB 010067), two gram-negative bacteria: Escherichia coli (RCMB 010052), and Proteus vulgaris RCMB 004 (1) ATCC 13315, and the results are depicted in and . ..

    Isolation:

    Article Title: Erubescensoic Acid, a New Polyketide and a Xanthonopyrone SPF-3059-26 from the Culture of the Marine Sponge-Associated Fungus Penicillium erubescens KUFA 0220 and Antibacterial Activity Evaluation of Some of Its Constituents
    Article Snippet: .. Gram-positive bacteria comprised Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, methicillin-resistant Staphylococcus aureus (MRSA) 66/1, isolated from public buses [ ] and vancomycin-resistant Enterococcus faecalis (VRE) B3/101, isolated from river water [ ]. ..

    Concentration Assay:

    Article Title: Eco-Friendly Synthesis, Characterization and Biological Evaluation of Some Novel Pyrazolines Containing Thiazole Moiety as Potential Anticancer and Antimicrobial Agents
    Article Snippet: .. Evaluation of the Antimicrobial Activity The in vitro antimicrobial activities of the newly synthesized compounds and reference drugs were tested by inhibition zone technique [ , ] and minimum inhibitory concentration (MIC), using two fungi: Aspergillus fumigatus (RCMB 002008 (4) and Candida albicans (RCMB 05036), two gram-positive bacteria: Staphylococcus aureus (RCMB 010010), and Bacillus subtilis (RCMB 010067), two gram-negative bacteria: Escherichia coli (RCMB 010052), and Proteus vulgaris RCMB 004 (1) ATCC 13315, and the results are depicted in and . ..

    Inhibition:

    Article Title: Eco-Friendly Synthesis, Characterization and Biological Evaluation of Some Novel Pyrazolines Containing Thiazole Moiety as Potential Anticancer and Antimicrobial Agents
    Article Snippet: .. Evaluation of the Antimicrobial Activity The in vitro antimicrobial activities of the newly synthesized compounds and reference drugs were tested by inhibition zone technique [ , ] and minimum inhibitory concentration (MIC), using two fungi: Aspergillus fumigatus (RCMB 002008 (4) and Candida albicans (RCMB 05036), two gram-positive bacteria: Staphylococcus aureus (RCMB 010010), and Bacillus subtilis (RCMB 010067), two gram-negative bacteria: Escherichia coli (RCMB 010052), and Proteus vulgaris RCMB 004 (1) ATCC 13315, and the results are depicted in and . ..

    Activity Assay:

    Article Title: An Efficient Approach to the Synthesis of Highly Congested 9,10-Dihydrophenanthrene-2,4-dicarbonitriles and Their Biological Evaluation as Antimicrobial Agents
    Article Snippet: .. The prepared compounds were tested for their antimicrobial activity against Staphylococcus aureus (ATCC 25923) as Gram positive bacteria, Escherichia coli (ATCC 25922) as Gram negative bacteria, and the antifungal activity was performed using the pathogenic yeast strains Candida albicans and Aspergillus niger . ..

    Article Title: Novel 5′-Norcarbocyclic Pyrimidine Derivatives as Antibacterial Agents
    Article Snippet: .. The antibacterial activity of the synthesized compounds was also evaluated against Gram-negative bacteria including Pseudomonas aeruginosa (P. aeruginosa ) ATCC strain 27853 and Escherichia coli (E. coli ) ATCC strain 25922, Gram-positive bacteria including Bacillus subtilis (B. subtilis) ATCC strain 6633, Staphylococcus aureus (S. aureus ) INA 00761 (MRSA) and Leuconostoc mesenteroides (L. mesenteroides ) VKPM B-4177. .. Mycobacterium smegmatis (M. smegmatis ) mc2 155 and M. smegmatis VKPM Ac 1339, were also used in the primary screening for potential antibiotic activity.

    Article Title: Eco-Friendly Synthesis, Characterization and Biological Evaluation of Some Novel Pyrazolines Containing Thiazole Moiety as Potential Anticancer and Antimicrobial Agents
    Article Snippet: .. Evaluation of the Antimicrobial Activity The in vitro antimicrobial activities of the newly synthesized compounds and reference drugs were tested by inhibition zone technique [ , ] and minimum inhibitory concentration (MIC), using two fungi: Aspergillus fumigatus (RCMB 002008 (4) and Candida albicans (RCMB 05036), two gram-positive bacteria: Staphylococcus aureus (RCMB 010010), and Bacillus subtilis (RCMB 010067), two gram-negative bacteria: Escherichia coli (RCMB 010052), and Proteus vulgaris RCMB 004 (1) ATCC 13315, and the results are depicted in and . ..

    Article Title: Antimicrobial activities of endophytic fungi of the Sri Lankan aquatic plant Nymphaea nouchali and chaetoglobosin A and C, produced by the endophytic fungus Chaetomium globosum
    Article Snippet: .. Antimicrobial MICs of chaetoglobosin A (1) and C (2) Antimicrobial activity of chaetoglobosin A and C was evaluated against three Gram-positive bacteria, B. subtilis (UBC 344), S. aureus (ATCC 43300) and methicillin-resistant S. aureus (MRSA, ATCC 33591), two Gram-negative bacteria, E. coli (UBC 8161) and P. aeruginosa (ATCC 27853), and the pathogenic fungus C. albicans (ATCC 90028). .. The minimum inhibitory concentrations (MICs) were determined using the broth micro-dilution method according to National Committee for Clinical Laboratory Standards with modification using Mueller Hinton broth as the medium (NCCLS ).

    Article Title: Marine Microalgae: Promising Source for New Bioactive Compounds
    Article Snippet: .. Antibacterial, Antifungal, and Antiviral Activity Extracts from biomass and cell free culture medium were evaluated for antimicrobial activity against two Gram-positive bacteria, Enterococcus faecalis (ATCC 29212) and Staphylococcus aureus (ATCC 25923), one Gram-negative bacterium, Escherichia coli (ATCC 25922) and a fungal strain, Candida albicans ( ATCC 90028). ..

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  • 99
    ATCC gram positive bacteria corynebacterium glutamicum
    Fluorescent images for detection of boundary between electroporated and non-electroporated bacteria. Fluorescent images ( a ) before and ( b ) after delivering a 1.8-kV exponentially decaying ( t = 1.0 ms; τ = 5.0 ms) pulse in 0.01× phosphate buffered saline (PBS) buffer (PBS diluted 100 times in DI water) and 5 μM SYTOX ® Green nucleic acid stain to C. <t>glutamicum</t> bacteria (scale bar = 200 μm).
    Gram Positive Bacteria Corynebacterium Glutamicum, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC gram positive bacteria staphylococcus aureus atcc 25923
    (A) SDS-PAGE electrophoresis of crude extract of Pseudoalteromonas sp and (B) gel developed by direct bioautography against S. aureus <t>ATCC</t> 25923 showing a single inhibitory zone. Clear zones of inhibition around the disks and strip of gel indicate antibacterial activity, (V) refers to disk impregnated with positive control vancomycin; (E) refers to disk impregnated with crude extract. The arrows show the position of molecular mass in kilodaltons.
    Gram Positive Bacteria Staphylococcus Aureus Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gram positive bacteria staphylococcus aureus atcc 25923/product/ATCC
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    Price from $9.99 to $1999.99
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    93
    ATCC gram positive bacterial species
    <t>Gram-positive</t> and gram-negative <t>bacterial</t> <t>species</t> growth in BHI/LB at different concentrations (0, 1, 2, 5, 10, 20, 40 μg/mL) of AgNPs.
    Gram Positive Bacterial Species, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gram positive bacterial species/product/ATCC
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Fluorescent images for detection of boundary between electroporated and non-electroporated bacteria. Fluorescent images ( a ) before and ( b ) after delivering a 1.8-kV exponentially decaying ( t = 1.0 ms; τ = 5.0 ms) pulse in 0.01× phosphate buffered saline (PBS) buffer (PBS diluted 100 times in DI water) and 5 μM SYTOX ® Green nucleic acid stain to C. glutamicum bacteria (scale bar = 200 μm).

    Journal: Scientific Reports

    Article Title: Microfluidic Screening of Electric Fields for Electroporation

    doi: 10.1038/srep21238

    Figure Lengend Snippet: Fluorescent images for detection of boundary between electroporated and non-electroporated bacteria. Fluorescent images ( a ) before and ( b ) after delivering a 1.8-kV exponentially decaying ( t = 1.0 ms; τ = 5.0 ms) pulse in 0.01× phosphate buffered saline (PBS) buffer (PBS diluted 100 times in DI water) and 5 μM SYTOX ® Green nucleic acid stain to C. glutamicum bacteria (scale bar = 200 μm).

    Article Snippet: As test cases, we characterize the gram-positive bacteria Corynebacterium glutamicum (ATCC 13032, Manassas, VA, USA) and Mycobacterium smegmatis (ATCC, Manassas, VA, USA), and the gram-negative strain Escherichia coli BL21 (Bioline competent cells BIO-85032, London, UK).

    Techniques: Mass Spectrometry, Staining

    Critical electric field ( E crit ) for bacterial electroporation as a function of applied voltage. Panel ( a ) shows the values obtained from individual experiments, visualizing the data shown in Table 1 ; panel ( b ) shows averages (error bars show ± Δ E crit ) for each bacterium at each applied voltage. These values indicate that (gram-negative) E. coli BL21 requires a smaller E crit than C. glutamicum and M. smegmatis (gram-positive) bacteria.

    Journal: Scientific Reports

    Article Title: Microfluidic Screening of Electric Fields for Electroporation

    doi: 10.1038/srep21238

    Figure Lengend Snippet: Critical electric field ( E crit ) for bacterial electroporation as a function of applied voltage. Panel ( a ) shows the values obtained from individual experiments, visualizing the data shown in Table 1 ; panel ( b ) shows averages (error bars show ± Δ E crit ) for each bacterium at each applied voltage. These values indicate that (gram-negative) E. coli BL21 requires a smaller E crit than C. glutamicum and M. smegmatis (gram-positive) bacteria.

    Article Snippet: As test cases, we characterize the gram-positive bacteria Corynebacterium glutamicum (ATCC 13032, Manassas, VA, USA) and Mycobacterium smegmatis (ATCC, Manassas, VA, USA), and the gram-negative strain Escherichia coli BL21 (Bioline competent cells BIO-85032, London, UK).

    Techniques: Electroporation

    ( a ) Summed intensity (a.u., arbitrary units) versus distance from the channel center. The summed intensity at each point is equal to the sum of the intensity values in the (shaded) vertical column of pixels located at the point of interest. ( b ) Result of the one-sided two-sample Kolmogorov-Smirnov (KS) test as a function of position along the channel. The value of H at a given location indicates that the null hypothesis H 0 was either accepted ( H = 0) or rejected ( H = 1) for a 51-pixel stencil centered at that location. As discussed in the main text, H = 1 for regions in which a significant portion of the post-pulse fluorescence intensity values exceed the pre-pulse values. In general, the location at which H changes from 0 to 1 is taken as the location of onset of fluorescence enhancement due to electroporation. For the case depicted above ( C. glutamicum , V app = 1.8 kV), the predicted values of the critical electric field (found by linearly interpolating the simulated electric field data visualized in Fig. 2a,b ) are 4.28 kV/cm (left) and 3.85 kV/cm (right), yielding an average E crit = 4.07 kV/cm. Note that in cases such as this, in which the entire channel is in the microscope’s field of view, it is possible to exploit the bilateral symmetry of the channel geometry and obtain two estimates of E crit – one on each end of the channel.

    Journal: Scientific Reports

    Article Title: Microfluidic Screening of Electric Fields for Electroporation

    doi: 10.1038/srep21238

    Figure Lengend Snippet: ( a ) Summed intensity (a.u., arbitrary units) versus distance from the channel center. The summed intensity at each point is equal to the sum of the intensity values in the (shaded) vertical column of pixels located at the point of interest. ( b ) Result of the one-sided two-sample Kolmogorov-Smirnov (KS) test as a function of position along the channel. The value of H at a given location indicates that the null hypothesis H 0 was either accepted ( H = 0) or rejected ( H = 1) for a 51-pixel stencil centered at that location. As discussed in the main text, H = 1 for regions in which a significant portion of the post-pulse fluorescence intensity values exceed the pre-pulse values. In general, the location at which H changes from 0 to 1 is taken as the location of onset of fluorescence enhancement due to electroporation. For the case depicted above ( C. glutamicum , V app = 1.8 kV), the predicted values of the critical electric field (found by linearly interpolating the simulated electric field data visualized in Fig. 2a,b ) are 4.28 kV/cm (left) and 3.85 kV/cm (right), yielding an average E crit = 4.07 kV/cm. Note that in cases such as this, in which the entire channel is in the microscope’s field of view, it is possible to exploit the bilateral symmetry of the channel geometry and obtain two estimates of E crit – one on each end of the channel.

    Article Snippet: As test cases, we characterize the gram-positive bacteria Corynebacterium glutamicum (ATCC 13032, Manassas, VA, USA) and Mycobacterium smegmatis (ATCC, Manassas, VA, USA), and the gram-negative strain Escherichia coli BL21 (Bioline competent cells BIO-85032, London, UK).

    Techniques: Fluorescence, Electroporation, Microscopy

    Image-processing methodology to compute the cumulative fluorescence intensity along the channel and enable rapid visual identification of the location of electroporated cells. ( a ) The cumulative intensity CI at a point (indicated by the black arrow) is defined as the sum of the red shaded pixel values. In this figure, the black “Background” curve is virtually indistinguishable from the red “Before” curve. ( b ) The adjusted cumulative intensity CI’ is the same as in ( a ) but with the background subtracted to accurately determine the location of the onset of electroporation along the channel, which is indicated with a red star in both subfigures. This figure allows visual identification of the onset of electroporation-induced fluorescence enhancement, and agrees with the quantitative findings of the KS test. Panels ( c , d ) are analogous to ( a,b ) for a case of C. glutamicum subjected to a 1-ms 2-kV voltage pulse.

    Journal: Scientific Reports

    Article Title: Microfluidic Screening of Electric Fields for Electroporation

    doi: 10.1038/srep21238

    Figure Lengend Snippet: Image-processing methodology to compute the cumulative fluorescence intensity along the channel and enable rapid visual identification of the location of electroporated cells. ( a ) The cumulative intensity CI at a point (indicated by the black arrow) is defined as the sum of the red shaded pixel values. In this figure, the black “Background” curve is virtually indistinguishable from the red “Before” curve. ( b ) The adjusted cumulative intensity CI’ is the same as in ( a ) but with the background subtracted to accurately determine the location of the onset of electroporation along the channel, which is indicated with a red star in both subfigures. This figure allows visual identification of the onset of electroporation-induced fluorescence enhancement, and agrees with the quantitative findings of the KS test. Panels ( c , d ) are analogous to ( a,b ) for a case of C. glutamicum subjected to a 1-ms 2-kV voltage pulse.

    Article Snippet: As test cases, we characterize the gram-positive bacteria Corynebacterium glutamicum (ATCC 13032, Manassas, VA, USA) and Mycobacterium smegmatis (ATCC, Manassas, VA, USA), and the gram-negative strain Escherichia coli BL21 (Bioline competent cells BIO-85032, London, UK).

    Techniques: Fluorescence, Electroporation, Mass Spectrometry

    (A) SDS-PAGE electrophoresis of crude extract of Pseudoalteromonas sp and (B) gel developed by direct bioautography against S. aureus ATCC 25923 showing a single inhibitory zone. Clear zones of inhibition around the disks and strip of gel indicate antibacterial activity, (V) refers to disk impregnated with positive control vancomycin; (E) refers to disk impregnated with crude extract. The arrows show the position of molecular mass in kilodaltons.

    Journal: Brazilian Journal of Microbiology

    Article Title: Oxygen limitation favors the production of protein with antimicrobial activity in Pseudoalteromonas sp

    doi: 10.1590/S1517-838220120003000048

    Figure Lengend Snippet: (A) SDS-PAGE electrophoresis of crude extract of Pseudoalteromonas sp and (B) gel developed by direct bioautography against S. aureus ATCC 25923 showing a single inhibitory zone. Clear zones of inhibition around the disks and strip of gel indicate antibacterial activity, (V) refers to disk impregnated with positive control vancomycin; (E) refers to disk impregnated with crude extract. The arrows show the position of molecular mass in kilodaltons.

    Article Snippet: Determination of antimicrobial activity Antibacterial activity of crude extract was tested by the standard disk diffusion (Kirby-Bauer) method ( , ), against clinical pathogens which included: Gram positive bacteria Staphylococcus aureus ATCC 25923 (a), Staphylococcus aureus (b) clinical isolates sensitive to ampicillin (AM), ceftazidime (CAZ), cefataxime (CXM), gentamicin (GM) and Staphylococcus aureus (c) MRSA clinical isolate resistant to AM, GM, CAZ, and Gram negative bacteria such as Escherichia coli ATCC 25922 (d) and Pseudomonas aeruginosa (e) clinical isolate resistant to cefataxime (CTX), amikacin (AN).

    Techniques: SDS Page, Electrophoresis, Inhibition, Stripping Membranes, Activity Assay, Positive Control

    Specific activity of the antimicrobial substance of crude extract of  Pseudoalteromonas  sp under different agitation intensities. Values are expressed as U/mg, against different test pathogens.  a Staphylococcus aureus  ATCC 25923;  b Staphylococcus aureus  clinical isolated sensible to AM, CAZ,CXM, GM;  c Staphylococcus aureus  (MRSA) clinical isolated resistant to AM, GM, CAZ;  d Escherichia coli  ATCC 25922;  e P.aeruginosa  clinical isolate resistant to cefataxime (CTX), amikacin (AN)

    Journal: Brazilian Journal of Microbiology

    Article Title: Oxygen limitation favors the production of protein with antimicrobial activity in Pseudoalteromonas sp

    doi: 10.1590/S1517-838220120003000048

    Figure Lengend Snippet: Specific activity of the antimicrobial substance of crude extract of Pseudoalteromonas sp under different agitation intensities. Values are expressed as U/mg, against different test pathogens. a Staphylococcus aureus ATCC 25923; b Staphylococcus aureus clinical isolated sensible to AM, CAZ,CXM, GM; c Staphylococcus aureus (MRSA) clinical isolated resistant to AM, GM, CAZ; d Escherichia coli ATCC 25922; e P.aeruginosa clinical isolate resistant to cefataxime (CTX), amikacin (AN)

    Article Snippet: Determination of antimicrobial activity Antibacterial activity of crude extract was tested by the standard disk diffusion (Kirby-Bauer) method ( , ), against clinical pathogens which included: Gram positive bacteria Staphylococcus aureus ATCC 25923 (a), Staphylococcus aureus (b) clinical isolates sensitive to ampicillin (AM), ceftazidime (CAZ), cefataxime (CXM), gentamicin (GM) and Staphylococcus aureus (c) MRSA clinical isolate resistant to AM, GM, CAZ, and Gram negative bacteria such as Escherichia coli ATCC 25922 (d) and Pseudomonas aeruginosa (e) clinical isolate resistant to cefataxime (CTX), amikacin (AN).

    Techniques: Activity Assay, Isolation

    Gram-positive and gram-negative bacterial species growth in BHI/LB at different concentrations (0, 1, 2, 5, 10, 20, 40 μg/mL) of AgNPs.

    Journal: Frontiers in Microbiology

    Article Title: Silver Nanoparticles at Biocompatible Dosage Synergistically Increases Bacterial Susceptibility to Antibiotics

    doi: 10.3389/fmicb.2020.01074

    Figure Lengend Snippet: Gram-positive and gram-negative bacterial species growth in BHI/LB at different concentrations (0, 1, 2, 5, 10, 20, 40 μg/mL) of AgNPs.

    Article Snippet: Gram-positive bacterial species included: Staphylococcus aureus ATCC 25923, Methicillin Resistant Staphylococcus aureus (MRSA) ATCC 4330, Streptococcus mutans ATCC 25175, Streptococcus oralis ATCC 35037, Streptococcus gordonii ATCC 49818, and Enterococcus faecalis 700802.

    Techniques: