gr p 20 antibodies  (Santa Cruz Biotechnology)

 
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    Name:
    TAF II p20 Antibody
    Description:
    Anti TAF II p20 Antibody B 6 is a mouse monoclonal IgG1 kappa light chain TAF II p20 antibody provided at 200 µg ml raised against amino acids 1 161 representing full length TAF II p20 of human origin Anti TAF II p20 Antibody B 6 is recommended for detection of TAF II p20 of mouse rat and human origin by WB IP IF and ELISA Anti TAF II p20 Antibody B 6 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of TAF II p20 B 6 sc 514619
    Catalog Number:
    SC-514619
    Price:
    None
    Category:
    Antibodies Primary Antibodies and ImmunoCruz Conjugates Transcription Regulators TAF II p20 Antibodies TAF II p20 Antibody B 6
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    Structured Review

    Santa Cruz Biotechnology gr p 20 antibodies
    Anti TAF II p20 Antibody B 6 is a mouse monoclonal IgG1 kappa light chain TAF II p20 antibody provided at 200 µg ml raised against amino acids 1 161 representing full length TAF II p20 of human origin Anti TAF II p20 Antibody B 6 is recommended for detection of TAF II p20 of mouse rat and human origin by WB IP IF and ELISA Anti TAF II p20 Antibody B 6 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of TAF II p20 B 6 sc 514619
    https://www.bioz.com/result/gr p 20 antibodies/product/Santa Cruz Biotechnology
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gr p 20 antibodies - by Bioz Stars, 2021-09
    88/100 stars

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    Article Title: Arabidopsis KANADI1 Acts as a Transcriptional Repressor by Interacting with a Specific cis-Element and Regulates Auxin Biosynthesis, Transport, and Signaling in Opposition to HD-ZIPIII Factors [W]
    Article Snippet: After chromatin shearing, 10 μL of anti-GR P-20 (Santa Cruz Biotechnology) was added to each sample to immunoprecipitate KAN-GR proteins.

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  • 93
    Santa Cruz Biotechnology grα
    Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The <t>GRα</t> and GRβ cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors αβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.
    Grα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grα/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    grα - by Bioz Stars, 2021-09
    93/100 stars
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    95
    Santa Cruz Biotechnology anti gr polyclonal antibodies
    Glucocorticoid receptor (GR) binding to p53 and p73 . A and B , Saos-2 cells (p53-null) were transfected overnight with 200 ng GR DNA and 100 ng DNA encoding either p53 or p73β. Where indicated, transfected cells were treated with dexamethasone (Dex, 100 nM) for 17 hrs and then incubated in the presence or absence of MG132 (30 μM) for an additional 6 hrs. GR, p53, and p73 levels were monitored by immunblotting. C , Saos-2 cells were transfected with DNAs encoding GR, HA-p53, or HA-p73β (1 μg each) as indicated. Transfected cells were either untreated (no tr), treated with dexamethasone (+Dex, 100 nM) for 24 hrs, treated with MG132 (30 μM) for 6 hrs, or treated with dexamethasone for 17 hrs followed by incubation in dexamethasone plus MG132 for an additional 6 hrs. Cell lysates were immunoprecipitated with GR <t>polyclonal</t> antibody, followed by immunoblotting with a HA monoclonal antibody. The position of HA-p53 ( left ) and HA-p73β ( right ) that co-immunoprecipitated with GR is indicated. The asterisk indicates detection of the antibody heavy chain used in the immunoprecipitation. Levels of GR, HA-p53, and HA-p73 in transfected cell lysates determined by immunoblotting without prior immunoprecipitation are shown in the lower panels.
    Anti Gr Polyclonal Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gr polyclonal antibodies/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gr polyclonal antibodies - by Bioz Stars, 2021-09
    95/100 stars
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    96
    Santa Cruz Biotechnology polyclonal rabbit anti grα antibodies
    Glucocorticoid receptor (GR) binding to p53 and p73 . A and B , Saos-2 cells (p53-null) were transfected overnight with 200 ng GR DNA and 100 ng DNA encoding either p53 or p73β. Where indicated, transfected cells were treated with dexamethasone (Dex, 100 nM) for 17 hrs and then incubated in the presence or absence of MG132 (30 μM) for an additional 6 hrs. GR, p53, and p73 levels were monitored by immunblotting. C , Saos-2 cells were transfected with DNAs encoding GR, HA-p53, or HA-p73β (1 μg each) as indicated. Transfected cells were either untreated (no tr), treated with dexamethasone (+Dex, 100 nM) for 24 hrs, treated with MG132 (30 μM) for 6 hrs, or treated with dexamethasone for 17 hrs followed by incubation in dexamethasone plus MG132 for an additional 6 hrs. Cell lysates were immunoprecipitated with GR <t>polyclonal</t> antibody, followed by immunoblotting with a HA monoclonal antibody. The position of HA-p53 ( left ) and HA-p73β ( right ) that co-immunoprecipitated with GR is indicated. The asterisk indicates detection of the antibody heavy chain used in the immunoprecipitation. Levels of GR, HA-p53, and HA-p73 in transfected cell lysates determined by immunoblotting without prior immunoprecipitation are shown in the lower panels.
    Polyclonal Rabbit Anti Grα Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti grα antibodies/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti grα antibodies - by Bioz Stars, 2021-09
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    88
    Santa Cruz Biotechnology gr p 20 antibodies
    Glucocorticoid receptor (GR) binding to p53 and p73 . A and B , Saos-2 cells (p53-null) were transfected overnight with 200 ng GR DNA and 100 ng DNA encoding either p53 or p73β. Where indicated, transfected cells were treated with dexamethasone (Dex, 100 nM) for 17 hrs and then incubated in the presence or absence of MG132 (30 μM) for an additional 6 hrs. GR, p53, and p73 levels were monitored by immunblotting. C , Saos-2 cells were transfected with DNAs encoding GR, HA-p53, or HA-p73β (1 μg each) as indicated. Transfected cells were either untreated (no tr), treated with dexamethasone (+Dex, 100 nM) for 24 hrs, treated with MG132 (30 μM) for 6 hrs, or treated with dexamethasone for 17 hrs followed by incubation in dexamethasone plus MG132 for an additional 6 hrs. Cell lysates were immunoprecipitated with GR <t>polyclonal</t> antibody, followed by immunoblotting with a HA monoclonal antibody. The position of HA-p53 ( left ) and HA-p73β ( right ) that co-immunoprecipitated with GR is indicated. The asterisk indicates detection of the antibody heavy chain used in the immunoprecipitation. Levels of GR, HA-p53, and HA-p73 in transfected cell lysates determined by immunoblotting without prior immunoprecipitation are shown in the lower panels.
    Gr P 20 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gr p 20 antibodies/product/Santa Cruz Biotechnology
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The GRα and GRβ cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors αβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The GRα and GRβ cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors αβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Western Blot, Expressing

    Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients . GRα and GRβ cell expression were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on ICU admission (first day in the ICU) and on the day of hospital discharge, which was considered as the control situation (free of sepsis). The quantification of the western blot analysis of (A) GRα and (B) GRβ is shown. The values were normalized to β actin expression and are expressed as percentage values of the control (GR expression on the day of hospital discharge). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). (C) Representative western blot analysis of GRαGRβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Western blot analysis of glucocorticoid receptor α and β isoforms of peripheral blood mononuclear cells from septic patients . GRα and GRβ cell expression were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on ICU admission (first day in the ICU) and on the day of hospital discharge, which was considered as the control situation (free of sepsis). The quantification of the western blot analysis of (A) GRα and (B) GRβ is shown. The values were normalized to β actin expression and are expressed as percentage values of the control (GR expression on the day of hospital discharge). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). (C) Representative western blot analysis of GRαGRβ and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Western Blot, Expressing

    Glucocorticoid receptor isoform expression on the first and third day in the intensive care unit for the survivors and non-survivors of sepsis . GRα and β cell expression were evaluated on the first and third days in the ICU by western blotting of peripheral blood mononuclear cells from septic patients who survived or did not survive the septic event. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in the ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GRα, β and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Glucocorticoid receptor isoform expression on the first and third day in the intensive care unit for the survivors and non-survivors of sepsis . GRα and β cell expression were evaluated on the first and third days in the ICU by western blotting of peripheral blood mononuclear cells from septic patients who survived or did not survive the septic event. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown. The values were normalized to β actin expression and are expressed as percentage values of the GR expression on the first day in the ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GRα, β and β actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Expressing, Western Blot

    Effect of human septic serum on glucocorticoid receptor isoform expression in different cell lines . CEM, Raji and K562 cell lines were cultured in the presence of the patients' septic serum and serum obtained from the control group at 10% final concentration in the culture medium. After 48 h in culture, the GR protein expression was analyzed by western blotting with specific antibodies for GRα and β isoforms. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown ( n = 9). The values were normalized to β actin expression and are expressed as percentage values of the control (GR cell expression cultured in the presence of control serum). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). Representative western blot analysis of GRα, β and β actin are shown for each cell line. CS, control serum; GR, glucocorticoid receptor; SS, septic serum.

    Journal: Critical Care

    Article Title: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

    doi: 10.1186/cc12774

    Figure Lengend Snippet: Effect of human septic serum on glucocorticoid receptor isoform expression in different cell lines . CEM, Raji and K562 cell lines were cultured in the presence of the patients' septic serum and serum obtained from the control group at 10% final concentration in the culture medium. After 48 h in culture, the GR protein expression was analyzed by western blotting with specific antibodies for GRα and β isoforms. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown ( n = 9). The values were normalized to β actin expression and are expressed as percentage values of the control (GR cell expression cultured in the presence of control serum). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). Representative western blot analysis of GRα, β and β actin are shown for each cell line. CS, control serum; GR, glucocorticoid receptor; SS, septic serum.

    Article Snippet: Western blot The expression of the cells' GR isoforms was evaluated by western blotting using specific antibodies for GRα (GR P-20: sc1002; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and GRβ (PA3-514; Affinity BioReagents, Golden, CO, USA), as described previously [ ].

    Techniques: Expressing, Cell Culture, Concentration Assay, Western Blot

    Glucocorticoid receptor (GR) binding to p53 and p73 . A and B , Saos-2 cells (p53-null) were transfected overnight with 200 ng GR DNA and 100 ng DNA encoding either p53 or p73β. Where indicated, transfected cells were treated with dexamethasone (Dex, 100 nM) for 17 hrs and then incubated in the presence or absence of MG132 (30 μM) for an additional 6 hrs. GR, p53, and p73 levels were monitored by immunblotting. C , Saos-2 cells were transfected with DNAs encoding GR, HA-p53, or HA-p73β (1 μg each) as indicated. Transfected cells were either untreated (no tr), treated with dexamethasone (+Dex, 100 nM) for 24 hrs, treated with MG132 (30 μM) for 6 hrs, or treated with dexamethasone for 17 hrs followed by incubation in dexamethasone plus MG132 for an additional 6 hrs. Cell lysates were immunoprecipitated with GR polyclonal antibody, followed by immunoblotting with a HA monoclonal antibody. The position of HA-p53 ( left ) and HA-p73β ( right ) that co-immunoprecipitated with GR is indicated. The asterisk indicates detection of the antibody heavy chain used in the immunoprecipitation. Levels of GR, HA-p53, and HA-p73 in transfected cell lysates determined by immunoblotting without prior immunoprecipitation are shown in the lower panels.

    Journal: Molecular Cancer

    Article Title: P53 and p73 differ in their ability to inhibit glucocorticoid receptor (GR) transcriptional activity

    doi: 10.1186/1476-4598-5-68

    Figure Lengend Snippet: Glucocorticoid receptor (GR) binding to p53 and p73 . A and B , Saos-2 cells (p53-null) were transfected overnight with 200 ng GR DNA and 100 ng DNA encoding either p53 or p73β. Where indicated, transfected cells were treated with dexamethasone (Dex, 100 nM) for 17 hrs and then incubated in the presence or absence of MG132 (30 μM) for an additional 6 hrs. GR, p53, and p73 levels were monitored by immunblotting. C , Saos-2 cells were transfected with DNAs encoding GR, HA-p53, or HA-p73β (1 μg each) as indicated. Transfected cells were either untreated (no tr), treated with dexamethasone (+Dex, 100 nM) for 24 hrs, treated with MG132 (30 μM) for 6 hrs, or treated with dexamethasone for 17 hrs followed by incubation in dexamethasone plus MG132 for an additional 6 hrs. Cell lysates were immunoprecipitated with GR polyclonal antibody, followed by immunoblotting with a HA monoclonal antibody. The position of HA-p53 ( left ) and HA-p73β ( right ) that co-immunoprecipitated with GR is indicated. The asterisk indicates detection of the antibody heavy chain used in the immunoprecipitation. Levels of GR, HA-p53, and HA-p73 in transfected cell lysates determined by immunoblotting without prior immunoprecipitation are shown in the lower panels.

    Article Snippet: Antibodies used in immunoblotting included anti-HA monoclonal antibody (HA.11, Covance), anti-Flag monoclonal antibody Ab-5 (Sigma-Aldrich), anti-GR polyclonal antibodies (E-20 and P-20, Santa Cruz Biotechnology), anti-MDM2 monoclonal antibody SMP-14 (Santa Cruz Biotechnology).

    Techniques: Binding Assay, Transfection, Incubation, Immunoprecipitation