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Miltenyi Biotec gr 1 cd11b mdsc
<t>Gr-1</t> + <t>CD11b</t> + cells coinjected with Pan02 tumor cells facilitate more rapid tumor engraftment
Gr 1 Cd11b Mdsc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gr 1 cd11b mdsc/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gr 1 cd11b mdsc - by Bioz Stars, 2021-09
97/100 stars

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1) Product Images from "Pancreatic adenocarcinoma induces bone marrow mobilization of myeloid derived suppressor cells which promote primary tumor growth"

Article Title: Pancreatic adenocarcinoma induces bone marrow mobilization of myeloid derived suppressor cells which promote primary tumor growth

Journal: Cancer immunology, immunotherapy : CII

doi: 10.1007/s00262-011-1178-0

Gr-1 + CD11b + cells coinjected with Pan02 tumor cells facilitate more rapid tumor engraftment
Figure Legend Snippet: Gr-1 + CD11b + cells coinjected with Pan02 tumor cells facilitate more rapid tumor engraftment

Techniques Used:

Related Articles

Isolation:

Article Title: The role of myeloid-derived suppressor cells in endometrial cancer displaying systemic inflammatory response: clinical and preclinical investigations
Article Snippet: .. Isolation of MDSCs MDSCs were isolated from the spleen of G-CSF-derived tumor-bearing Balb/c nude mice using a Myeloid-Derived Suppressor Cell Isolation Kit and an MS column (Miltenyi Biotec, Auburn, CA, USA). ..

Article Title: MDSC and TGF-β are required for facilitation of tumor growth in the lungs of mice exposed to carbon nanotubes
Article Snippet: .. CD11b+ Gr-1+ MDSC were isolated from the lungs of WT and TGF-β-deficient mice exposed to SWCNT or saline by magnetic cell sorting using a mouse MDSC Isolation Kit (MACS, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions. ..

Article Title: Bone marrow mesenchymal stem cells tune the differentiation of myeloid-derived suppressor cells in bleomycin-induced lung injury
Article Snippet: .. CD3+ cells were isolated from spleens of naïve C57BL/6 mice using anti-CD3 magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) and plated at 2 × 105 cells/well in 1 μg/mL of rat anti-mouse CD3 mAb (BD Pharmingen)-coated plates. ..

Article Title: SOCS3 Suppression Promoted the Recruitment of CD11b+Gr-1−F4/80−MHCII− Early-Stage Myeloid-Derived Suppressor Cells and Accelerated Interleukin-6-Related Tumor Invasion via Affecting Myeloid Differentiation in Breast Cancer
Article Snippet: .. After erythrocytolysis, CD11b+ Gr-1+ MDSCs were isolated using beads conjugated with biotin anti-mouse Gr-1 and anti-biotin microbeads (Miltenyi Biotec, Germany), and CD11b+ Gr-1− MDSCs were isolated using anti-mouse CD11b microbeads after CD11b+ Gr-1+ MDSCs were removed. ..

Article Title: Pancreatic adenocarcinoma induces bone marrow mobilization of myeloid derived suppressor cells which promote primary tumor growth
Article Snippet: .. 100,000 CFSE-labeled splenocytes obtained from OT-1 mice were co-cultured in 96-well plates with 1μm SIINFEKL peptide and varying concentrations of Gr-1+ CD11b+ MDSC isolated from mature tumors, spleen, or bone marrow after successful CD11b+ magnetic bead separation (Miltenyi Biotech), according to the manufacturer’s instructions. ..

Mouse Assay:

Article Title: The role of myeloid-derived suppressor cells in endometrial cancer displaying systemic inflammatory response: clinical and preclinical investigations
Article Snippet: .. Isolation of MDSCs MDSCs were isolated from the spleen of G-CSF-derived tumor-bearing Balb/c nude mice using a Myeloid-Derived Suppressor Cell Isolation Kit and an MS column (Miltenyi Biotec, Auburn, CA, USA). ..

Article Title: MDSC and TGF-β are required for facilitation of tumor growth in the lungs of mice exposed to carbon nanotubes
Article Snippet: .. CD11b+ Gr-1+ MDSC were isolated from the lungs of WT and TGF-β-deficient mice exposed to SWCNT or saline by magnetic cell sorting using a mouse MDSC Isolation Kit (MACS, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions. ..

Article Title: Bone marrow mesenchymal stem cells tune the differentiation of myeloid-derived suppressor cells in bleomycin-induced lung injury
Article Snippet: .. CD3+ cells were isolated from spleens of naïve C57BL/6 mice using anti-CD3 magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) and plated at 2 × 105 cells/well in 1 μg/mL of rat anti-mouse CD3 mAb (BD Pharmingen)-coated plates. ..

Article Title: Pancreatic adenocarcinoma induces bone marrow mobilization of myeloid derived suppressor cells which promote primary tumor growth
Article Snippet: .. 100,000 CFSE-labeled splenocytes obtained from OT-1 mice were co-cultured in 96-well plates with 1μm SIINFEKL peptide and varying concentrations of Gr-1+ CD11b+ MDSC isolated from mature tumors, spleen, or bone marrow after successful CD11b+ magnetic bead separation (Miltenyi Biotech), according to the manufacturer’s instructions. ..

Article Title: Tumor-induced MDSC act via remote control to inhibit L-selectin-dependent adaptive immunity in lymph nodes
Article Snippet: .. T cell suppression assays To evaluate T cell suppression during continuous exposure to MDSC, splenic CD11b+ Gr-1+ cells were purified from non-tumor bearing (NTB) and 4T1-bearing BALB/c mice (tumor volume > 2000 mm3 ) using anti-CD11b+ magnetic beads (Miltenyi Biotec, San Diego, CA; ) as described ( ). ..

Cell Isolation:

Article Title: The role of myeloid-derived suppressor cells in endometrial cancer displaying systemic inflammatory response: clinical and preclinical investigations
Article Snippet: .. Isolation of MDSCs MDSCs were isolated from the spleen of G-CSF-derived tumor-bearing Balb/c nude mice using a Myeloid-Derived Suppressor Cell Isolation Kit and an MS column (Miltenyi Biotec, Auburn, CA, USA). ..

FACS:

Article Title: MDSC and TGF-β are required for facilitation of tumor growth in the lungs of mice exposed to carbon nanotubes
Article Snippet: .. CD11b+ Gr-1+ MDSC were isolated from the lungs of WT and TGF-β-deficient mice exposed to SWCNT or saline by magnetic cell sorting using a mouse MDSC Isolation Kit (MACS, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions. ..

Magnetic Cell Separation:

Article Title: MDSC and TGF-β are required for facilitation of tumor growth in the lungs of mice exposed to carbon nanotubes
Article Snippet: .. CD11b+ Gr-1+ MDSC were isolated from the lungs of WT and TGF-β-deficient mice exposed to SWCNT or saline by magnetic cell sorting using a mouse MDSC Isolation Kit (MACS, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions. ..

Staining:

Article Title: Overexpression of Dominant Negative Peroxisome Proliferator-Activated Receptor-? (PPAR?) in Alveolar Type II Epithelial Cells Causes Inflammation and T-Cell Suppression in the Lung
Article Snippet: .. To purify CD11b+ Gr-1+ MDSCs, single cells from the bone marrow, spleen, and lung were stained with anti-Gr-1+ -biotin antibodies, followed by positive magnetic selection using anti-biotin microbeads according to the manufacturer's instructions (Miltenyi Biotec). ..

Selection:

Article Title: Overexpression of Dominant Negative Peroxisome Proliferator-Activated Receptor-? (PPAR?) in Alveolar Type II Epithelial Cells Causes Inflammation and T-Cell Suppression in the Lung
Article Snippet: .. To purify CD11b+ Gr-1+ MDSCs, single cells from the bone marrow, spleen, and lung were stained with anti-Gr-1+ -biotin antibodies, followed by positive magnetic selection using anti-biotin microbeads according to the manufacturer's instructions (Miltenyi Biotec). ..

Magnetic Beads:

Article Title: Bone marrow mesenchymal stem cells tune the differentiation of myeloid-derived suppressor cells in bleomycin-induced lung injury
Article Snippet: .. CD3+ cells were isolated from spleens of naïve C57BL/6 mice using anti-CD3 magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) and plated at 2 × 105 cells/well in 1 μg/mL of rat anti-mouse CD3 mAb (BD Pharmingen)-coated plates. ..

Article Title: Tumor-induced MDSC act via remote control to inhibit L-selectin-dependent adaptive immunity in lymph nodes
Article Snippet: .. T cell suppression assays To evaluate T cell suppression during continuous exposure to MDSC, splenic CD11b+ Gr-1+ cells were purified from non-tumor bearing (NTB) and 4T1-bearing BALB/c mice (tumor volume > 2000 mm3 ) using anti-CD11b+ magnetic beads (Miltenyi Biotec, San Diego, CA; ) as described ( ). ..

Purification:

Article Title: Tumor-induced MDSC act via remote control to inhibit L-selectin-dependent adaptive immunity in lymph nodes
Article Snippet: .. T cell suppression assays To evaluate T cell suppression during continuous exposure to MDSC, splenic CD11b+ Gr-1+ cells were purified from non-tumor bearing (NTB) and 4T1-bearing BALB/c mice (tumor volume > 2000 mm3 ) using anti-CD11b+ magnetic beads (Miltenyi Biotec, San Diego, CA; ) as described ( ). ..

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    Miltenyi Biotec gr 1 cd11b mdsc
    <t>Gr-1</t> + <t>CD11b</t> + cells coinjected with Pan02 tumor cells facilitate more rapid tumor engraftment
    Gr 1 Cd11b Mdsc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gr 1 cd11b mdsc/product/Miltenyi Biotec
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gr 1 cd11b mdsc - by Bioz Stars, 2021-09
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec cd11b gr 1 mdsc
    TGF-β deficiency does not abrogate acute accumulation of <t>MDSC</t> in the lung and spleen after a single pulmonary exposure to SWCNT Wild type (WT) and Tgfb1 tm1Doe mice received SWCNT by pharyngeal inhalation and the levels of monocytic <t>CD11b</t> + Ly6G neg Ly6C high (area 1) and granulocytic CD11b + Ly6G + Ly6C low/neg (area 2) MDSC were assessed by flow cytometry in the lymphoid tissues and lungs. The results of flow cytometry analysis of MDSC accumulation in tumor-free Tgfb1 tm1Doe mice 48h after SWCNT aspiration are shown from a representative experiment ( a ) (CD11b+ gated cells are shown). Statistical analysis of data from 3 independent experiments (5-6 mice/group in each) is shown as the mean ± SEM and demonstrates accumulation of polymorphonuclear MDSC in different tissues in tumor-free mice 48 h after SWCNT aspiration. *, p
    Cd11b Gr 1 Mdsc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd11b gr 1 mdsc/product/Miltenyi Biotec
    Average 97 stars, based on 1 article reviews
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    Miltenyi Biotec mdscs suppression assay cd11b gr 1
    The role of AMPK in the miR-10a expression of <t>MDSCs</t> induced by PGE2. ( A ) Western blotting of p-AMPK and AMPK in BM-derived MDSCs cultured with conditioned medium from 4T1 or Doxorubicin-resistant 4T1 cells (4T1/DOX) for 3 days in the presence of GM-CSF and IL-6. ( B ) BM-derived MDSCs transfected with miR-10a antagomir (miR-10a ASO) or scrambled control were cultured with metformin (10mM) or compound C (Com C, 5 μM) in the presence of or absence of PGE 2 , and the expression of miR-10a was determined by qRT-PCR. ( C ) BM-derived MDSCs transfected with miR-10a mimics or scrambled control were cultured with metformin (10 mM) or Compound C (Com C, 5 μM) in the presence of or absence of PGE 2 , MMP9 and Arg1 mRNA were determined by qRT-PCR. ( D ) MDSCs were cultured as C and activated by LPS (100 ng/ml) and IFN-γ (2 ng/ml), the secretion of IL-10 was examined by ELISA. ( E ) Representative FACS plots showing the percentage of M-MDSCs and G-MDSCs (cells were gated on <t>CD11b</t> + <t>Gr-1</t> + ) in spleen two weeks after 4T1/DOX cells were intravenously inoculated into BALB/c mice with/without Compound C treatment (25 mg/kg, one time/every three days for four times). ( F ) Quantification of the expression of miR-10 in CD11b + Gr-1 + MDSCs sorted from spleen. Data represent Mean ± SD from 3 individual experiments. ** p
    Mdscs Suppression Assay Cd11b Gr 1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mdscs suppression assay cd11b gr 1/product/Miltenyi Biotec
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    Miltenyi Biotec mdsc isolation total mdscs
    NFAT5 silencing inhibited high-salt-mediated <t>M-MDSC</t> differentiation and functional transformation. a , b Purified tumour <t>M-MDSCs</t> were transfected with NFAT5-specific siRNA and cultured in the absence or presence of an additional 40 mM NaCl for 3 days. Populations of CD11b + F4/80 + cells were tested by flow cytometry. c , d Purified tumour M-MDSCs were transfected with NFAT5-specific siRNA for 2 days and cultured in the absence or presence of an additional 40 mM NaCl for 24 h. The expression of IL-12, TNF-α and IL-10 in the supernatant was determined by ELISA. NOS2 and Arg1 mRNA expression levels were evaluated by qRT-PCR. e – g p-JNK was analysed by western blotting; quantification data of western blotting and NF-κB activity were determined by the p65 subunit DNA-binding ability. h NFAT5 expression was analysed by western blotting. Right panels: quantification data. For all panels, * p
    Mdsc Isolation Total Mdscs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Gr-1 + CD11b + cells coinjected with Pan02 tumor cells facilitate more rapid tumor engraftment

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Pancreatic adenocarcinoma induces bone marrow mobilization of myeloid derived suppressor cells which promote primary tumor growth

    doi: 10.1007/s00262-011-1178-0

    Figure Lengend Snippet: Gr-1 + CD11b + cells coinjected with Pan02 tumor cells facilitate more rapid tumor engraftment

    Article Snippet: 100,000 CFSE-labeled splenocytes obtained from OT-1 mice were co-cultured in 96-well plates with 1μm SIINFEKL peptide and varying concentrations of Gr-1+ CD11b+ MDSC isolated from mature tumors, spleen, or bone marrow after successful CD11b+ magnetic bead separation (Miltenyi Biotech), according to the manufacturer’s instructions.

    Techniques:

    TGF-β deficiency does not abrogate acute accumulation of MDSC in the lung and spleen after a single pulmonary exposure to SWCNT Wild type (WT) and Tgfb1 tm1Doe mice received SWCNT by pharyngeal inhalation and the levels of monocytic CD11b + Ly6G neg Ly6C high (area 1) and granulocytic CD11b + Ly6G + Ly6C low/neg (area 2) MDSC were assessed by flow cytometry in the lymphoid tissues and lungs. The results of flow cytometry analysis of MDSC accumulation in tumor-free Tgfb1 tm1Doe mice 48h after SWCNT aspiration are shown from a representative experiment ( a ) (CD11b+ gated cells are shown). Statistical analysis of data from 3 independent experiments (5-6 mice/group in each) is shown as the mean ± SEM and demonstrates accumulation of polymorphonuclear MDSC in different tissues in tumor-free mice 48 h after SWCNT aspiration. *, p

    Journal: Cancer research

    Article Title: MDSC and TGF-β are required for facilitation of tumor growth in the lungs of mice exposed to carbon nanotubes

    doi: 10.1158/0008-5472.CAN-14-2376

    Figure Lengend Snippet: TGF-β deficiency does not abrogate acute accumulation of MDSC in the lung and spleen after a single pulmonary exposure to SWCNT Wild type (WT) and Tgfb1 tm1Doe mice received SWCNT by pharyngeal inhalation and the levels of monocytic CD11b + Ly6G neg Ly6C high (area 1) and granulocytic CD11b + Ly6G + Ly6C low/neg (area 2) MDSC were assessed by flow cytometry in the lymphoid tissues and lungs. The results of flow cytometry analysis of MDSC accumulation in tumor-free Tgfb1 tm1Doe mice 48h after SWCNT aspiration are shown from a representative experiment ( a ) (CD11b+ gated cells are shown). Statistical analysis of data from 3 independent experiments (5-6 mice/group in each) is shown as the mean ± SEM and demonstrates accumulation of polymorphonuclear MDSC in different tissues in tumor-free mice 48 h after SWCNT aspiration. *, p

    Article Snippet: CD11b+ Gr-1+ MDSC were isolated from the lungs of WT and TGF-β-deficient mice exposed to SWCNT or saline by magnetic cell sorting using a mouse MDSC Isolation Kit (MACS, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry

    The role of AMPK in the miR-10a expression of MDSCs induced by PGE2. ( A ) Western blotting of p-AMPK and AMPK in BM-derived MDSCs cultured with conditioned medium from 4T1 or Doxorubicin-resistant 4T1 cells (4T1/DOX) for 3 days in the presence of GM-CSF and IL-6. ( B ) BM-derived MDSCs transfected with miR-10a antagomir (miR-10a ASO) or scrambled control were cultured with metformin (10mM) or compound C (Com C, 5 μM) in the presence of or absence of PGE 2 , and the expression of miR-10a was determined by qRT-PCR. ( C ) BM-derived MDSCs transfected with miR-10a mimics or scrambled control were cultured with metformin (10 mM) or Compound C (Com C, 5 μM) in the presence of or absence of PGE 2 , MMP9 and Arg1 mRNA were determined by qRT-PCR. ( D ) MDSCs were cultured as C and activated by LPS (100 ng/ml) and IFN-γ (2 ng/ml), the secretion of IL-10 was examined by ELISA. ( E ) Representative FACS plots showing the percentage of M-MDSCs and G-MDSCs (cells were gated on CD11b + Gr-1 + ) in spleen two weeks after 4T1/DOX cells were intravenously inoculated into BALB/c mice with/without Compound C treatment (25 mg/kg, one time/every three days for four times). ( F ) Quantification of the expression of miR-10 in CD11b + Gr-1 + MDSCs sorted from spleen. Data represent Mean ± SD from 3 individual experiments. ** p

    Journal: Scientific Reports

    Article Title: Doxorubicin resistant cancer cells activate myeloid-derived suppressor cells by releasing PGE2

    doi: 10.1038/srep23824

    Figure Lengend Snippet: The role of AMPK in the miR-10a expression of MDSCs induced by PGE2. ( A ) Western blotting of p-AMPK and AMPK in BM-derived MDSCs cultured with conditioned medium from 4T1 or Doxorubicin-resistant 4T1 cells (4T1/DOX) for 3 days in the presence of GM-CSF and IL-6. ( B ) BM-derived MDSCs transfected with miR-10a antagomir (miR-10a ASO) or scrambled control were cultured with metformin (10mM) or compound C (Com C, 5 μM) in the presence of or absence of PGE 2 , and the expression of miR-10a was determined by qRT-PCR. ( C ) BM-derived MDSCs transfected with miR-10a mimics or scrambled control were cultured with metformin (10 mM) or Compound C (Com C, 5 μM) in the presence of or absence of PGE 2 , MMP9 and Arg1 mRNA were determined by qRT-PCR. ( D ) MDSCs were cultured as C and activated by LPS (100 ng/ml) and IFN-γ (2 ng/ml), the secretion of IL-10 was examined by ELISA. ( E ) Representative FACS plots showing the percentage of M-MDSCs and G-MDSCs (cells were gated on CD11b + Gr-1 + ) in spleen two weeks after 4T1/DOX cells were intravenously inoculated into BALB/c mice with/without Compound C treatment (25 mg/kg, one time/every three days for four times). ( F ) Quantification of the expression of miR-10 in CD11b + Gr-1 + MDSCs sorted from spleen. Data represent Mean ± SD from 3 individual experiments. ** p

    Article Snippet: MDSCs suppression assay CD11b+ Gr-1+ MDSCs were purified using magnetic microbeads (Miltenyi Biotec) or from FACS sorting.

    Techniques: Expressing, Western Blot, Derivative Assay, Cell Culture, Transfection, Allele-specific Oligonucleotide, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, FACS, Mouse Assay

    PGE 2 promotes the expansion and M2 polarization of MDSCs via miR-10a. BM cells were treated with PGE 2 in the presence of GM-CSF and IL-6. MiR-10a antagomir (miR-10a ASO) and scrambled oligonucleotides were transfected on the second day. Gr-1 + CD11b + MDSCs were evaluated by flowcytometry after 4 days ( A ). BM cells in ( A ) were cultured with ( B ) or without ( C ) EP4 antagonist (1 μM ONO-AE3-208), rhe relative levels of TNF-α, NOS2, Arg1, MMP9, and TGF-β mRNA in BM-derived MDSCs were detected by qRT-PCR after 3 days of transfection; ( D ) In vitro suppressive ability of MDSCs that transfected with miR-10a antagomir (miR-10a ASO) or scrambled control on naïve CD4 + CD25 − T cells proliferation were analyzed by flowcytometry. CFSE labeled CD4 + CD25 − naïve T cells were incubated with APC (CD4 + T cell depleted splenocytes) and MDSCs, and stimulated with anti-CD3 with/without PGE 2 for 3–5 days. Cell proliferation was measured as a function of CFSE dilution. ( E ) The level of IFN-γ in the supernatant of cocultured cells in D was determined by ELISA. Data represent Mean ± SD from 3–5 individual experiments. *p

    Journal: Scientific Reports

    Article Title: Doxorubicin resistant cancer cells activate myeloid-derived suppressor cells by releasing PGE2

    doi: 10.1038/srep23824

    Figure Lengend Snippet: PGE 2 promotes the expansion and M2 polarization of MDSCs via miR-10a. BM cells were treated with PGE 2 in the presence of GM-CSF and IL-6. MiR-10a antagomir (miR-10a ASO) and scrambled oligonucleotides were transfected on the second day. Gr-1 + CD11b + MDSCs were evaluated by flowcytometry after 4 days ( A ). BM cells in ( A ) were cultured with ( B ) or without ( C ) EP4 antagonist (1 μM ONO-AE3-208), rhe relative levels of TNF-α, NOS2, Arg1, MMP9, and TGF-β mRNA in BM-derived MDSCs were detected by qRT-PCR after 3 days of transfection; ( D ) In vitro suppressive ability of MDSCs that transfected with miR-10a antagomir (miR-10a ASO) or scrambled control on naïve CD4 + CD25 − T cells proliferation were analyzed by flowcytometry. CFSE labeled CD4 + CD25 − naïve T cells were incubated with APC (CD4 + T cell depleted splenocytes) and MDSCs, and stimulated with anti-CD3 with/without PGE 2 for 3–5 days. Cell proliferation was measured as a function of CFSE dilution. ( E ) The level of IFN-γ in the supernatant of cocultured cells in D was determined by ELISA. Data represent Mean ± SD from 3–5 individual experiments. *p

    Article Snippet: MDSCs suppression assay CD11b+ Gr-1+ MDSCs were purified using magnetic microbeads (Miltenyi Biotec) or from FACS sorting.

    Techniques: Allele-specific Oligonucleotide, Transfection, Cell Culture, Derivative Assay, Quantitative RT-PCR, In Vitro, Labeling, Incubation, Enzyme-linked Immunosorbent Assay

    NFAT5 silencing inhibited high-salt-mediated M-MDSC differentiation and functional transformation. a , b Purified tumour M-MDSCs were transfected with NFAT5-specific siRNA and cultured in the absence or presence of an additional 40 mM NaCl for 3 days. Populations of CD11b + F4/80 + cells were tested by flow cytometry. c , d Purified tumour M-MDSCs were transfected with NFAT5-specific siRNA for 2 days and cultured in the absence or presence of an additional 40 mM NaCl for 24 h. The expression of IL-12, TNF-α and IL-10 in the supernatant was determined by ELISA. NOS2 and Arg1 mRNA expression levels were evaluated by qRT-PCR. e – g p-JNK was analysed by western blotting; quantification data of western blotting and NF-κB activity were determined by the p65 subunit DNA-binding ability. h NFAT5 expression was analysed by western blotting. Right panels: quantification data. For all panels, * p

    Journal: Nature Communications

    Article Title: High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation

    doi: 10.1038/s41467-020-15524-1

    Figure Lengend Snippet: NFAT5 silencing inhibited high-salt-mediated M-MDSC differentiation and functional transformation. a , b Purified tumour M-MDSCs were transfected with NFAT5-specific siRNA and cultured in the absence or presence of an additional 40 mM NaCl for 3 days. Populations of CD11b + F4/80 + cells were tested by flow cytometry. c , d Purified tumour M-MDSCs were transfected with NFAT5-specific siRNA for 2 days and cultured in the absence or presence of an additional 40 mM NaCl for 24 h. The expression of IL-12, TNF-α and IL-10 in the supernatant was determined by ELISA. NOS2 and Arg1 mRNA expression levels were evaluated by qRT-PCR. e – g p-JNK was analysed by western blotting; quantification data of western blotting and NF-κB activity were determined by the p65 subunit DNA-binding ability. h NFAT5 expression was analysed by western blotting. Right panels: quantification data. For all panels, * p

    Article Snippet: MDSC isolation Total MDSCs (T-MDSCs, CD11b+ Gr-1+ ), M-MDSCs (CD11b+ Ly6-C+ ) and PMN-MDSCs (CD11b+ Ly6-G+ ) were isolated from the tumour tissues by the Myeloid-Derived Suppressor Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany), with all steps performed at 4 °C.

    Techniques: Functional Assay, Transformation Assay, Purification, Transfection, Cell Culture, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Activity Assay, Binding Assay

    HSD promoted MDSC differentiation and functional transformation. a , b M-MDSCs purified in tumour tissues from the NSD or HSD group were cultured in RPMI-1640 medium containing 10% FBS and 10 ng ml −1 GM-CSF for 2, 3 or 4 days. Population of CD11b + F4/80 + cells was evaluated by flow cytometry. One-way ANOVA with post hoc Bonferroni correction; n = 10 mice per group; * p

    Journal: Nature Communications

    Article Title: High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation

    doi: 10.1038/s41467-020-15524-1

    Figure Lengend Snippet: HSD promoted MDSC differentiation and functional transformation. a , b M-MDSCs purified in tumour tissues from the NSD or HSD group were cultured in RPMI-1640 medium containing 10% FBS and 10 ng ml −1 GM-CSF for 2, 3 or 4 days. Population of CD11b + F4/80 + cells was evaluated by flow cytometry. One-way ANOVA with post hoc Bonferroni correction; n = 10 mice per group; * p

    Article Snippet: MDSC isolation Total MDSCs (T-MDSCs, CD11b+ Gr-1+ ), M-MDSCs (CD11b+ Ly6-C+ ) and PMN-MDSCs (CD11b+ Ly6-G+ ) were isolated from the tumour tissues by the Myeloid-Derived Suppressor Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany), with all steps performed at 4 °C.

    Techniques: Functional Assay, Transformation Assay, Purification, Cell Culture, Flow Cytometry, Mouse Assay